当前位置： SCI文献检索 > JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS期刊下所有文献 > Assay of artemether, methylparaben and propylparaben in a formulated paediatric antimalarial dry suspension.

Assay of artemether, methylparaben and propylparaben in a formulated paediatric antimalarial dry suspension.

Abstract：

:Two HPLC-UV methods are described for the separate determination of artemether (AM) and the combined preservatives, methylparaben and propylparaben in a pharmaceutical dosage form. These analytes are contained in a dry suspension with a high amount of non-soluble excipients, some of which can interfere with the analysis. This makes their separation and analysis of the actives complex. Moreover, due to the wide difference in concentrations, the three analytes could not be quantitated simultaneously. Artemether was analysed using a reversed-phase Nucleosil C(18) column [5 microm, 125 mm x 4 mm (i.d.)] with a mixture of acetonitrile: potassium phosphate buffer pH 5.0 (0.05 M): water [48:32:10 (v/v/v)] as mobile phase. Due to the low solubility of the hydroxy benzoic acid esters in water, their sodium salts were used in the formulation. Complete separation of these preservatives was achieved on the same type of column as artemether using as eluent acetonitrile: potassium phosphate buffer pH 5.0 (0.05 M) (30:70, v/v). Quantitation was achieved with UV detection at 215 nm for artemether and 254 nm for the parabens, respectively. And in both methods, pump flow rate was 1.0 ml/min, sample injection volume 20 microl, ambient temperature maintained and no prior sample extraction methods were necessary throughout the experiments. Calibration curves were linear at concentration ranges of 4-16 microg/ml, 1-4 microg/ml and 1-10 mg/ml for methylparaben, propylparaben and artemether respectively. The excipient powder interference could be eliminated by diluting the sample and the analytes eluted at relatively short times using these systems. Both methods were further validated in terms of specificity, linearity, precision and accuracy. The procedures prescribed here are simple, selective and can be used for routine quality control and stability indicating tests involving the analysed compounds formulated in complex matrices.

journal_name

J Pharm Biomed Anal

journal_title

Journal of pharmaceutical and biomedical analysis

authors

Atemnkeng MA,Marchand E,Plaizier-Vercammen J

doi

10.1016/j.jpba.2006.07.053

subject

Has Abstract

pub_date

2007-01-17 00:00:00

pages

727-32

issue

eissn

0731-7085

issn

1873-264X

pii

S0731-7085(06)00647-9

journal_volume

pub_type

杂志文章

在线工具

Assay of artemether, methylparaben and propylparaben in a formulated paediatric antimalarial dry suspension.