Abstract:
:A rapid analytical procedure for routine identification and quantification of amoxicillin in premixes by high performance liquid chromatography was developed and tested. The ground premix samples were extracted for 10 min using 100ml extraction mixture water-methanol (800:200, v/v). The extract was analyzed by reversed-phase on Agilent Zorbax SB-C18 column (4.6 mm x 150 mm, i.d., 5 microm particle size) with water-methanol-phosphoric acid-triethylamine (842:150:4:4) containing 10 mM hexane-1-sulfonic acid sodium salt (pH 3.5) as mobile phase. UV detection was carried out at 230 nm. The method was validated for specificity, linearity, solution stability, accuracy, precision, limit of detection, and limit of determination. The detector response for amoxicillin was linear over the selected concentration range from 2.0 to 40.0 mg ml(-1) with a correlation coefficient 0.9999. The mean accuracy was 100.1% with a standard deviation of 0.6%. The limit of detection and the limit of determination are 0.1 and 0.3 mg ml(-1), respectively, which corresponds to 10 and 30 mg kg(-1), respectively, in real premix sample. The sample and standard solutions were stable for 4 h. The method is selective and can be used in routine analysis.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Dousa M,Hosmanová Rdoi
10.1016/j.jpba.2004.10.010subject
Has Abstractpub_date
2005-02-23 00:00:00pages
373-7issue
2eissn
0731-7085issn
1873-264Xpii
S0731-7085(04)00499-6journal_volume
37pub_type
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journal_title:Journal of pharmaceutical and biomedical analysis
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更新日期:2007-11-05 00:00:00
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journal_title:Journal of pharmaceutical and biomedical analysis
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pub_type: 杂志文章
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journal_title:Journal of pharmaceutical and biomedical analysis
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更新日期:2016-05-10 00:00:00
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章,评审
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journal_title:Journal of pharmaceutical and biomedical analysis
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journal_title:Journal of pharmaceutical and biomedical analysis
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journal_title:Journal of pharmaceutical and biomedical analysis
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abstract::Fentanyl, a potent analgesic drug, has traditionally been used intravenously in surgical or diagnostic operations. Formulations with fentanyl in oral transmucosal delivery system and in transdermal depot-patch have also been developed against breakthrough pain in cancer patients. In this report, LC-MS/MS methods to de...
journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
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journal_title:Journal of pharmaceutical and biomedical analysis
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journal_title:Journal of pharmaceutical and biomedical analysis
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journal_title:Journal of pharmaceutical and biomedical analysis
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journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
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journal_title:Journal of pharmaceutical and biomedical analysis
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abstract::A quick and sensitive method has been developed and validated for the determination of tungsten in bulk drug substance and intermediates using either Inductively Coupled Plasma Atomic Emission Spectrometry (ICP-AES) or Inductively Coupled Plasma Mass Spectrometry (ICP-MS). Sample preparation is by direct dissolution w...
journal_title:Journal of pharmaceutical and biomedical analysis
pub_type: 杂志文章
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