Abstract：
:A sensitive, rapid and selective liquid chromatography-tandem mass spectrometry (LC-MS-MS) method was developed and validated for the simultaneous determination of baicalin, baicalein, wogonin, berberine, palmatine and jatrorrhizine in scutellaria-coptis herb couple in rat plasma. After protein precipitation with acetonitrile and 0.1% NaH(2)PO(4), chromatography was performed using a C(18) column, with gradient elution with 0.1% formic acid and acetonitrile at 0.25 ml/min. All analytes including internal standards were monitored under positive ionization conditions by selected reaction monitoring with an electrospray ionization source. The lower limit of quantification was 10 ng/ml for baicalin, baicalein and wogonin, and 0.6 ng/ml for berberine, palmatine and jatrorrhizine. The validated method was applied in pharmacokinetic studies after oral administration of Yiqing Capsule and Gegen-Qinlian Tablet to rats.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Feng J,Xu W,Tao X,Wei H,Cai F,Jiang B,Chen Wdoi
10.1016/j.jpba.2010.04.002subject
Has Abstractpub_date
2010-11-02 00:00:00pages
591-8issue
3eissn
0731-7085issn
1873-264Xpii
S0731-7085(10)00228-1journal_volume
53pub_type
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