Abstract：
:This review paper discusses the success of columns packed with superficially porous particles (SPP) in liquid chromatography for the analysis of peptides and proteins. First, it summarizes the history of SPP, including the development of different SPP generations from particles of 50 μm to sub-2 μm. It also critically discusses the improved kinetic performance of SPP particles in comparison to fully porous particles. The current trends and applications of columns packed with SPPs for the analysis of peptides and proteins (including mAbs and ADC at the intact and sub-unit levels) are shown, as well. Finally, some of the potential perspectives for this technology are also described, including the radially oriented mesopores or the applicability of the technology for chiral separations.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Bobály B,Veuthey JL,Guillarme D,Fekete Sdoi
10.1016/j.jpba.2018.06.006subject
Has Abstractpub_date
2018-09-05 00:00:00pages
225-235eissn
0731-7085issn
1873-264Xpii
S0731-7085(18)31081-1journal_volume
158pub_type
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