Electron microscopic study of corneal epithelial-endothelial interactions in organ culture.

Abstract:

:The interactions between rabbit corneal epithelium and endothelium were investigated in an organ culture model using scanning electron microscopy and correlative light microscopy. Following mechanical removal of corneal endothelium, corneal epithelium was observed to migrate across the cut surface of the corneal stroma and onto the denuded Descemet's membrane after 48 hours in organ culture. By 72 hours, a distinct line of contact between the migrated epithelium and endothelium was established. Following epithelial-endothelial contact, no cellular migration occurred. Corneal endothelial inhibition of epithelial migration may be a major factor in the prevention of surface epithelial invasion and proliferation following surgery and trauma.

journal_name

Ophthalmology

journal_title

Ophthalmology

authors

Sassani JW,John T,Cameron JD,Yanoff M,Eagle RC Jr

doi

10.1016/s0161-6420(84)34250-6

subject

Has Abstract

pub_date

1984-06-01 00:00:00

pages

553-7

issue

6

eissn

0161-6420

issn

1549-4713

pii

S0161-6420(84)34250-6

journal_volume

91

pub_type

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