Abstract:
:Human neutrophils isolated from the blood were incubated in vitro for 30 min with 25 ng/ml of phorbol 12-myristate 13-acetate (PMA), a protein kinase C activator, to study its effect on the fine structure of granulocytes and on the subcellular distribution of lactoferrin (Lf). Flow cytometry analysis of the human neutrophils showed that PMA induced a decrease in size and granularity of the cells. By electron microscopy, the PMA-treated cells showed numerous empty vesicles, smoothing of the cell surface, and a marked decrease in the compactness of the cytoplasmic ground substance. High-resolution immunogold ultracytochemistry showed that neutrophils lost most of their content in Lf after the PMA incubation. In thin sections, the cytoplasmic compartment of PMA-treated neutrophils contained an average of 1.03 +/- 0.3 Lf-positive vesicles per microns 2, whereas control granulocytes showed an average of 5.72 +/- 1.49 Lf vesicles per microns 2 of cytoplasm. Most of PMA-treated neutrophils kept some Lf-positive vesicles; a significant minority (10-15%) of the granulocytes showed cytological features of cell death. We conclude that the PMA-induced neutrophil activation is associated with microanatomical changes that include i) exocytosis of most, but not all, of the Lf-bearing vesicles; ii) rounding up of the cell outline; and iii) decrease in the compactness of the cytoplasmic ground substance.
journal_name
J Leukoc Bioljournal_title
Journal of leukocyte biologyauthors
Esaguy N,Aguas AP,Vilanova M,Silva MTdoi
10.1002/jlb.50.5.444subject
Has Abstractpub_date
1991-11-01 00:00:00pages
444-52issue
5eissn
0741-5400issn
1938-3673journal_volume
50pub_type
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