Enrichment, solubilization, and partial characterization of digitonin-solubilized muscarinic receptors derived from canine ventricular myocardium.

abstract：RATIONALE:One of the physiological mechanisms by which the heart adapts to a rise in blood pressure is by augmenting myocyte stretch-mediated intracellular calcium, with a subsequent increase in contractility. This slow force response was first described over a century ago and has long been considered compensatory, but...

journal_title：Circulation research

authors： Cingolani OH,Kirk JA,Seo K,Koitabashi N,Lee DI,Ramirez-Correa G,Bedja D,Barth AS,Moens AL,Kass DA

更新日期：2011-12-09 00:00:00

abstract：:To investigate the contributions of humoral and hemodynamic factors to cardiac adaptations associated with chronic exercise, female Fischer 344 rats were subjected to chronic swimming, infrarenal cardiac transplantation, or both. Swimming resulted in hypertrophy (11-12%) of the in situ hearts in both the unoperated an...

journal_title：Circulation research

authors： Advani SV,Geenen D,Malhotra A,Factor SM,Scheuer J

更新日期：1990-09-01 00:00:00

abstract：:Vascular calcification is a common finding in atherosclerosis and a serious problem in diabetic and uremic patients. Because of the correlation of hyperphosphatemia and vascular calcification, the ability of extracellular inorganic phosphate levels to regulate human aortic smooth muscle cell (HSMC) culture mineralizat...

journal_title：Circulation research

authors： Jono S,McKee MD,Murry CE,Shioi A,Nishizawa Y,Mori K,Morii H,Giachelli CM

更新日期：2000-09-29 00:00:00

abstract：RATIONALE:Circumstantial evidence links the development of heart failure to posttranslational modifications of mitochondrial proteins, including lysine acetylation (Kac). Nonetheless, direct evidence that Kac compromises mitochondrial performance remains sparse. OBJECTIVE:This study sought to explore the premise that ...

journal_title：Circulation research

authors： Davidson MT,Grimsrud PA,Lai L,Draper JA,Fisher-Wellman KH,Narowski TM,Abraham DM,Koves TR,Kelly DP,Muoio DM

更新日期：2020-09-25 00:00:00

abstract：:Understanding molecular mechanisms regulating angiogenesis may lead to novel therapies for ischemic disorders. Hypoxia-inducible factor 1 (HIF-1) activates vascular endothelial growth factor (VEGF) gene expression in hypoxic/ischemic tissue. In this study we demonstrate that exposure of primary cultures of cardiac and...

journal_title：Circulation research

authors： Kelly BD,Hackett SF,Hirota K,Oshima Y,Cai Z,Berg-Dixon S,Rowan A,Yan Z,Campochiaro PA,Semenza GL

更新日期：2003-11-28 00:00:00

abstract：RATIONALE:The low-density lipoprotein (LDL) receptor (LDLR) is a central determinant of circulating LDL-cholesterol and as such subject to tight regulation. Recent studies and genetic evidence implicate the inducible degrader of the LDLR (IDOL) as a regulator of LDLR abundance and of circulating levels of LDL-cholester...

journal_title：Circulation research

authors： Nelson JK,Sorrentino V,Avagliano Trezza R,Heride C,Urbe S,Distel B,Zelcer N

更新日期：2016-02-05 00:00:00

abstract：:Activity was recorded from physiologically identified baroreceptor or chemoreceptor fibers in carotid sinus nerves of urethane-anesthetized spontaneously breathing rabbits. A carotid sinus area was vascularly isolated so that carotid sinus pressure and perfusion medium (Locke's solution or rabbit blood) could be contr...

journal_title：Circulation research

authors： Qu L,Stuesse SL

更新日期：1991-12-01 00:00:00

abstract：:Although human atherosclerosis is associated with aging, direct evidence of cellular senescence and the mechanism of senescence in vascular smooth muscle cells (VSMCs) in atherosclerotic plaques is lacking. We examined normal vessels and plaques by histochemistry, Southern blotting, and fluorescence in situ hybridizat...

journal_title：Circulation research

authors： Matthews C,Gorenne I,Scott S,Figg N,Kirkpatrick P,Ritchie A,Goddard M,Bennett M

更新日期：2006-07-21 00:00:00

abstract：:To isolate specific markers of both differentiated and proliferating vascular smooth muscle cells (VSMCs), we used the technique of differential cDNA screening using RNA from cultured rat aortic VSMCs. The tissue specificity of expression of all of the cDNAs isolated was determined by Northern analysis. We isolated se...

journal_title：Circulation research

authors： Shanahan CM,Weissberg PL,Metcalfe JC

更新日期：1993-07-01 00:00:00

abstract：:During cardiac development, the epicardium is the source of multipotent mesenchymal cells, which give rise to endothelial and smooth muscle cells in coronary vessels and also, possibly, to cardiomyocytes. The aim of the present study was to determine whether stem cells are retained in the adult human and murine epicar...

journal_title：Circulation research

authors： Limana F,Zacheo A,Mocini D,Mangoni A,Borsellino G,Diamantini A,De Mori R,Battistini L,Vigna E,Santini M,Loiaconi V,Pompilio G,Germani A,Capogrossi MC

更新日期：2007-12-07 00:00:00

abstract：:This article examines the role of innervation in regulating expression of acetylcholinesterase (AchE), butyrylcholinesterase (BuchE), and the muscarinic acetylcholine receptor (mAchR) in avian heart. Two distinct approaches are taken. The first approach examines the relation between the onsets of parasympathetic and s...

journal_title：Circulation research

authors： Jo SA,Higgins DM,Berman HA

更新日期：1992-04-01 00:00:00

abstract：:Hypertrophied cardiac myocytes exhibit prolonged action potentials and decreased transient outward potassium current (I(to)). Because Kv4.3 is a major contributor to I:(to), we studied regulation of its expression in neonatal rat cardiac myocytes in response to the known stimulators of cardiac myocyte hypertrophy, ang...

journal_title：Circulation research

authors： Zhang TT,Takimoto K,Stewart AF,Zhu C,Levitan ES

更新日期：2001-03-16 00:00:00

abstract：:The goals of this article were (1) to identify cell surface proteins whose expression was regulated by diabetes and (2) to assess their contribution to diabetic complications. We purified heat shock protein 90alpha (Hsp90alpha) from the membrane fraction of high glucose-treated endothelial cells (ECs) as a binding par...

journal_title：Circulation research

authors： Lei H,Romeo G,Kazlauskas A

更新日期：2004-04-16 00:00:00

abstract：:Hypercholesterolemia induces atheroma formation, and the concentration of sphingomyelin is increased compared with that in the normal aortic intima-media. The aortic intima-media sphingomyelin concentration appears to increase exponentially with time on the cholesterol diet. Moreover, the entry rate of serum sphingomy...

journal_title：Circulation research

authors： Seth SK,Newman AI

更新日期：1975-02-01 00:00:00

abstract：:Left ventricular (LV) remodeling after experimental myocardial infarction (MI) is associated with hypertrophy of noninfarcted myocardium and electrophysiological alterations. We have recently shown that post-MI hypertrophied LV myocytes have prolonged action potential duration (APD) and generate triggered activity fro...

journal_title：Circulation research

authors： Gidh-Jain M,Huang B,Jain P,el-Sherif N

更新日期：1996-10-01 00:00:00

abstract：:This study was performed to examine, first, the protective effects and responses of collateral vessels of the hind limb in normal and atherosclerotic monkeys and, second, the effects of chronic arterial occlusion on the development of atherosclerosis. The iliac artery was ligated on one side in cynomolgus monkeys. Six...

journal_title：Circulation research

authors： Lopez JA,Armstrong ML,Harrison DG,Piegors DJ,Heistad DD

更新日期：1988-12-01 00:00:00

abstract：:Our previous studies suggested that enhanced fibronectin mRNA translation in ductus arteriosus compared with aortic smooth muscle cells is related to increased expression of light chain 3 (LC3) of microtubule-associated protein 1, which binds an AU-rich element in the 3' untranslated region of fibronectin mRNA. We the...

journal_title：Circulation research

authors： Zhou B,Rabinovitch M

更新日期：1998-09-07 00:00:00

abstract：:The relationship between cell swelling and plasma membrane disruption has been evaluated in thin myocardial slices incubated in oxygenated or anoxic Krebs-Ringer phosphate media. Electron microscopy and measurements of inulin-diffusible space were used to monitor plasma membrane integrity. Inulin is excluded from the ...

journal_title：Circulation research

authors： Steenbergen C,Hill ML,Jennings RB

更新日期：1985-12-01 00:00:00

abstract：:The relations between atrial natriuretic peptide (ANP) binding sites in the renal medulla, plasma ANP concentration, and ventricular dysfunction have been studied in rats 4 weeks after myocardial infarction induced by left coronary artery ligation. Plasma ANP concentration was measured by radioimmunoassay, and quantit...

journal_title：Circulation research

authors： Tsunoda K,Mendelsohn FA,Sexton PM,Chai SY,Hodsman GP,Johnston CI

更新日期：1988-01-01 00:00:00

abstract：:L-type Ca(2+) channel (LTCC) blockers, represented by amlodipine and verapamil, are widely used antihypertensive drugs that also have antiinflammatory activities. Plasminogen (Plg) is an important mediator of macrophage recruitment, and this role depends on its interaction with Plg receptors (Plg-Rs). Plg-Rs include h...

journal_title：Circulation research

authors： Das R,Burke T,Van Wagoner DR,Plow EF

更新日期：2009-07-17 00:00:00

abstract：:Nuclear factor kappa B (NF-kappaB) participates in hypertension-induced vascular and target-organ damage. We tested whether or not endothelial cell-specific NF-kappaB suppression would be ameliorative. We generated Cre/lox transgenic mice with endothelial cell-restricted NF-kappaB super-repressor IkappaBalphaDeltaN (T...

journal_title：Circulation research

authors： Henke N,Schmidt-Ullrich R,Dechend R,Park JK,Qadri F,Wellner M,Obst M,Gross V,Dietz R,Luft FC,Scheidereit C,Muller DN

更新日期：2007-08-03 00:00:00

abstract：:Excitation-contraction coupling in the cardiac myocyte is mediated by a number of highly integrated mechanisms of intracellular Ca²(+) transport. The complexity and integrative nature of heart cell electrophysiology and Ca²(+) cycling has led to an evolution of computational models that have played a crucial role in s...

journal_title：Circulation research

authors： Greenstein JL,Winslow RL

更新日期：2011-01-07 00:00:00

abstract：:We developed a mouse myocardial preparation to study cellular dysfunction in acute coxsackievirus myocarditis. Thin right ventricular papillary muscles from normal mice (n = 8) were compared with muscles from mice 7 days after coxsackievirus infection (n = 7). Sarcomere shortening was studied with laser diffraction (H...

journal_title：Circulation research

authors： Hamrell BB,Huber SA,Leslie KO

更新日期：1994-09-01 00:00:00

abstract：:Arteries from deoxycorticosterone acetate (DOCA)-salt and N(omega)-nitro-L-arginine (L-NNA) hypertensive but not normotensive rats develop spontaneous tone. LY294002 and wortmannin, phosphoinositide 3-kinase (PI3-kinase) inhibitors, eliminate spontaneous tone. We hypothesized that PI3-kinase protein and/or activity wa...

journal_title：Circulation research

authors： Northcott CA,Poy MN,Najjar SM,Watts SW

更新日期：2002-08-23 00:00:00

abstract：:The focal pattern of atherosclerotic lesions in arterial vessels suggests that local blood flow patterns are important factors in atherosclerosis. Although disturbed flows in the branches and curved regions are proatherogenic, laminar flows in the straight parts are atheroprotective. Results from in vitro studies on c...

journal_title：Circulation research

authors： Shyy JY,Chien S

更新日期：2002-11-01 00:00:00

abstract：:Mutations in the human gene encoding the nucleotide-binding region in the gamma-subunit of AMP-activated protein kinase (AMPK) cause cardiomyopathy with preexcitation syndrome. Mutant AMPK showed reduced binding affinity to nucleotides in vitro raising the possibility that altered regulation of AMPK activity by AMP/AT...

journal_title：Circulation research

authors： Zou L,Shen M,Arad M,He H,Løfgren B,Ingwall JS,Seidman CE,Seidman JG,Tian R

更新日期：2005-08-19 00:00:00

abstract：:Increased glomerular capillary hydrostatic pressure (PGC) is an important hemodynamic determinant of glomerular injury, but the molecular events responsible for this association are poorly understood. PGC is normal in spontaneously hypertensive rats (SHR), but uninephrectomy leads to an increase in PGC and accelerated...

journal_title：Circulation research

authors： Shankland SJ,Ly H,Thai K,Scholey JW

更新日期：1994-11-01 00:00:00

abstract：:We examined the role of endogenous NO in the autonomic regulation of atrioventricular (AV) nodal function by studying spontaneous action potentials (SAPs) and L-type Ca2+ current (ICa-L) in isolated single AV nodal cells from adult rabbit hearts. Both the perforated and the membrane-ruptured patch-clamp techniques in ...

journal_title：Circulation research

authors： Han X,Kobzik L,Balligand JL,Kelly RA,Smith TW

更新日期：1996-06-01 00:00:00

abstract：:To see how serotonin affects filtration from lung vessels, we measured vascular pressures, lung lymph flow, lung lymph and blood plasma protein concentrations, arterial blood gases, cardiac output, and lung water content in unanesthetized sheep before and during intravenous serotonin infusions and compared serotonin e...

journal_title：Circulation research

authors： Brigham KL,Owen PJ

更新日期：1975-06-01 00:00:00

abstract：:The role of the Na+-Ca2+ exchanger as a major determinant of cell Ca2+ is well defined in cardiac tissue, and there has been much effort to develop specific inhibitors of the exchanger. We use a novel system to test the specificity of two putative specific inhibitors, KB-R7943 and SEA0400. The drugs are applied to ele...

journal_title：Circulation research

authors： Reuter H,Henderson SA,Han T,Matsuda T,Baba A,Ross RS,Goldhaber JI,Philipson KD

更新日期：2002-07-26 00:00:00