Abstract:
:The relationship between cell swelling and plasma membrane disruption has been evaluated in thin myocardial slices incubated in oxygenated or anoxic Krebs-Ringer phosphate media. Electron microscopy and measurements of inulin-diffusible space were used to monitor plasma membrane integrity. Inulin is excluded from the intracellular space of intact cells; therefore, an increase in tissue inulin content is an excellent marker of loss of plasma membrane integrity. Cell volume was increased during exposure of aerobic slices to hypotonic media, but the inulin-diffusible space was not increased and electron micrographs showed no detectable plasma membrane alterations. Likewise, during prolonged anoxic isotonic incubation, no evidence of plasma membrane damage was observed. Incubation in anoxic hypotonic media for 60 minutes resulted in a larger increase in cell volume than under aerobic conditions, but plasma membrane integrity was maintained. Extended anoxic hypotonic incubation (300 minutes) produced no further change in tissue water, but the inulin-diffusible space was increased and electron micrographs revealed breaks in the plasma membranes primarily in association with large subsarcolemmal blebs. Likewise, myocardial slices incubated in isotonic anoxic media for 240 minutes and hypotonic anoxic media for 60 minutes had an increased inulin-diffusible space and the ultrastructural appearance was similar. This ultrastructural appearance is indistinguishable from that observed in myocytes lethally injured by ischemia. Measurements of tissue osmolarity during total ischemia showed that osmotically induced cell swelling could occur in ischemic myocardium prior to the onset of plasma membrane disruption. Our results indicate that cell swelling per se is incapable of rupturing plasma membranes; however, after prolonged periods of energy deficiency, the plasma membrane or its cytoskeletal scaffold become injured, which allows the membrane to rupture if the cell is swollen, as might occur during ischemia or reperfusion.
journal_name
Circ Resjournal_title
Circulation researchauthors
Steenbergen C,Hill ML,Jennings RBdoi
10.1161/01.res.57.6.864subject
Has Abstractpub_date
1985-12-01 00:00:00pages
864-75issue
6eissn
0009-7330issn
1524-4571journal_volume
57pub_type
杂志文章abstract::Available data demonstrate that oxygen free radicals and derived reactive species of oxygen are produced during myocardial ischemia as well as upon reperfusion of the ischemic tissue. The present study was designed to determine if polyethylene glycol-conjugated superoxide dismutase (PEG-SOD), with its extended plasma ...
journal_title:Circulation research
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2013-07-19 00:00:00
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更新日期:2004-06-11 00:00:00
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pub_type: 杂志文章,收录出版
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更新日期:1981-02-01 00:00:00
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更新日期:1995-10-01 00:00:00
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更新日期:2005-07-08 00:00:00
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pub_type: 杂志文章
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更新日期:2008-05-23 00:00:00
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更新日期:1997-09-01 00:00:00
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更新日期:2005-09-02 00:00:00
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doi:10.1161/01.res.84.1.113
更新日期:1999-01-08 00:00:00
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pub_type: 杂志文章,评审
doi:10.1161/CIRCRESAHA.115.306883
更新日期:2016-05-27 00:00:00
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更新日期:1997-11-01 00:00:00
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更新日期:2002-11-15 00:00:00
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更新日期:2004-04-30 00:00:00
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更新日期:2016-02-05 00:00:00
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更新日期:1986-09-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2000-11-10 00:00:00
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更新日期:2012-09-28 00:00:00
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更新日期:1996-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1987-06-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:1975-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1991-10-01 00:00:00