Abstract:
:To elucidate the mechanisms responsible for the increase in alpha 1-adrenergic receptors during ischemia in vivo, we developed a procedure for measuring alpha 1-adrenergic receptors in isolated, calcium-tolerant adult canine myocytes. Specific [3H]prazosin binding was rapid, saturable, reversible, and demonstrated the expected order of potency and stereospecificity for the alpha 1-adrenergic receptor. Myocytes exposed to 30 minutes of hypoxia at 25 degrees C or only 10 minutes at 37 degrees C exhibited a twofold to threefold increase in the number of alpha 1-adrenergic receptors with no significant change in receptor affinity. This hypoxia-induced increase in receptor number was reversible by 10 minutes of reoxygenation at 37 degrees C. In contrast, more prolonged hypoxia of 80 minutes or hypotonic shock actually decreased receptor number below normoxic, control values. The concentration of long-chain acylcarnitines in myocytes also increased threefold on exposure to 30 minutes of hypoxia. Sodium 2-[5-(4-chlorophenyl)-pentyl]-oxirane-2-carboxylate (POCA, 10 microM), a potent inhibitor of carnitine acyltransferase I, not only abolished the accumulation of long-chain acylcarnitines but also the increase in alpha 1-adrenergic receptor number induced by 30 minutes of hypoxia. Likewise, incubation of normoxic cells with exogenous palmitoyl carnitine (1 microM) for 10 minutes also increased alpha 1-adrenergic receptor number in the presence or absence of POCA. Thus, hypoxia results in an increase in alpha 1-adrenergic receptors associated with an increase in endogenous long-chain acylcarnitines. Furthermore, inhibition of carnitine acyltransferase I prevents not only the sarcolemmal accumulation of long-chain acylcarnitines but also the exposure of the alpha 1-adrenergic receptor, indicating that accumulation of endogenous long-chain acylcarnitines is critical to the hypoxia-induced increase in alpha 1-adrenergic receptors on adult myocytes.
journal_name
Circ Resjournal_title
Circulation researchauthors
Heathers GP,Yamada KA,Kanter EM,Corr PBdoi
10.1161/01.res.61.5.735subject
Has Abstractpub_date
1987-11-01 00:00:00pages
735-46issue
5eissn
0009-7330issn
1524-4571journal_volume
61pub_type
杂志文章abstract::Structure/function analysis shows that the carboxyl terminal (CT) domain of connexin43 (Cx43) is essential for the chemical regulation of cell-cell communication. Of particular interest is the region between amino acids 260 and 300. Structural preservation of this region is essential for acidification-induced uncoupli...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.82.9.929
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pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.111.252734
更新日期:2012-03-16 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.72.5.1082
更新日期:1993-05-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.71.2.376
更新日期:1992-08-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2015-04-10 00:00:00
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pub_type: 历史文章,杂志文章,评审
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更新日期:2013-08-02 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.63.1.267
更新日期:1988-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.107.158329
更新日期:2007-08-31 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.41.6.787
更新日期:1977-12-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.69.3.757
更新日期:1991-09-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.71.4.870
更新日期:1992-10-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.RES.0000224120.52792.10
更新日期:2006-06-09 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.36.6.771
更新日期:1975-06-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.73.4.671
更新日期:1993-10-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.112.270132
更新日期:2012-09-28 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.63.6.1020
更新日期:1988-12-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.119.315833
更新日期:2019-12-06 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.62.5.975
更新日期:1988-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-11-21 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.39.6.860
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更新日期:1998-03-09 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:1998-06-15 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.83.1.73
更新日期:1998-07-13 00:00:00
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pub_type: 杂志文章
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更新日期:2018-05-25 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.37.4.481
更新日期:1975-10-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.RES.0000107196.21335.2B
更新日期:2004-01-09 00:00:00
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pub_type: 杂志文章
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更新日期:2003-03-21 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.50.2.294
更新日期:1982-02-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.111.240655
更新日期:2011-04-01 00:00:00