Abstract:
:The goals of this article were (1) to identify cell surface proteins whose expression was regulated by diabetes and (2) to assess their contribution to diabetic complications. We purified heat shock protein 90alpha (Hsp90alpha) from the membrane fraction of high glucose-treated endothelial cells (ECs) as a binding partner for a diabetes-specific phage. Further investigation revealed that high glucose elevated cell surface Hsp90alpha in cultured cells, and that diabetes increased the amount of Hsp90alpha on the luminal surface of the aorta. We also found that high glucose or diabetes promoted the association of Hsp90alpha with annexin II and increased the expression of annexin II on the surface of aortic ECs. Finally, plasmin activity was increased by high glucose or diabetes, and this change was partially reversed with an annexin II antibody. These findings reveal a novel glucose-regulated interaction between Hsp90alpha and annexin II, and raise the possibility that increased expression of annexin II, which promotes the generation of plasmin, is linked to clotting abnormalities associated with the diabetic state.
journal_name
Circ Resjournal_title
Circulation researchauthors
Lei H,Romeo G,Kazlauskas Adoi
10.1161/01.RES.0000124979.46214.E3subject
Has Abstractpub_date
2004-04-16 00:00:00pages
902-9issue
7eissn
0009-7330issn
1524-4571pii
01.RES.0000124979.46214.E3journal_volume
94pub_type
杂志文章abstract::The past 2 decades have witnessed a >40% improvement in mortality for patients with heart failure and left ventricular systolic dysfunction. 1 This success has coincided with the stepwise availability of drugs that target neurohormonal activation: β-adrenergic receptor blockers (β-blockers), ACE (angiotensin-convertin...
journal_title:Circulation research
pub_type: 杂志文章,评审
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abstract::Hemodynamics play an important role in cardiovascular development, and changes in blood flow can cause congenital heart malformations. The endothelium and endocardium are subjected to mechanical forces, of which fluid shear stress is correlated to blood flow velocity. The shear stress responsive genes lung Krüppel-lik...
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.74.3.416
更新日期:1994-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-02-01 00:00:00
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更新日期:1996-07-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.81.2.282
更新日期:1997-08-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.49.2.442
更新日期:1981-08-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:2012-03-16 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.39.3.400
更新日期:1976-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-01-07 00:00:00
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pub_type: 杂志文章
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更新日期:2007-03-16 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:1994-02-01 00:00:00
abstract::By use of gene targeting and/or transgenesis, it is now possible to make defined changes in genes whose functions underlie mammalian cardiovascular function. Because of technical and economic considerations, these experiments are largely confined to the mouse. Genetic modification of the loci responsible for aspects o...
journal_title:Circulation research
pub_type: 杂志文章,评审
doi:10.1161/01.res.82.4.407
更新日期:1998-03-09 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.70.2.438
更新日期:1992-02-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.74.4.641
更新日期:1994-04-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.0000019580.64013.31
更新日期:2002-05-31 00:00:00
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pub_type: 杂志文章
doi:10.1161/01.res.76.4.600
更新日期:1995-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1976-06-01 00:00:00
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更新日期:1995-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1987-08-01 00:00:00
abstract::Increasing evidence suggests that endogenous NO inhibits sympathetic outflow in anesthetized animals. However, in a recent study from this laboratory, we were unable to find any evidence of increased renal sympathetic nerve activity (RSNA) in response to blockade of NO synthesis in conscious rabbits. Because angiotens...
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更新日期:1998-03-09 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.36.6.761
更新日期:1975-06-01 00:00:00
abstract::Disorders of L-type Ca2+ channels can cause severe cardiac arrhythmias. A subclass of small GTP-binding proteins, the RGK family, regulates L-type Ca2+ current (I(Ca,L)) in heterologous expression systems. Among these proteins, Rad (Ras associated with diabetes) is highly expressed in the heart, although its role in t...
journal_title:Circulation research
pub_type: 杂志文章
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更新日期:2007-07-06 00:00:00
abstract::The red cell membrane is a permeability barrier that limits the equilibration of a variety of solutes between red cell and plasma water. We utilized the multiple indicator dilution technique to investigate the effect of this barrier on the exchange in the liver of a group of tracer substances that are not removed in n...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.36.2.328
更新日期:1975-02-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:
更新日期:2000-06-23 00:00:00
abstract::Mast cells in rat hearts were studied quantitatively during normal postnatal growth and in two types of cardiac hypertrophy. Normally, cardiac mast cell density in 11-12-day-old animals is very low, but increases markedly in the following 2-3 weeks to its highest values, with a subsequent decline toward adult values. ...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.66.2.511
更新日期:1990-02-01 00:00:00
abstract:RATIONALE:Mitochondria are key integrators of convergent intracellular signaling pathways. Two important second messengers modulated by mitochondria are calcium and reactive oxygen species. To date, coherent mechanisms describing mitochondrial integration of calcium and oxidative signaling in arterial smooth muscle are...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.115.306996
更新日期:2015-12-04 00:00:00