Abstract:
:To study the effects of podocyte injury on glomerular maturation and underlying mechanisms of such effects, puromycin aminonucleoside (PAN) was given to neonatal mice at 1 d post partum (1 dpp). Mice with PAN injection had smaller kidney weight (KW) and body weight (BW) at all times and smaller KW/BW at 4, 8, and 12 dpp versus normal saline (NS) controls. Electron microscopy (EM) revealed nearly complete podocyte foot process effacement and segmental microvillous transformation as early as 2 dpp, preceding proteinuria. PAN-injected kidneys showed significantly fewer glomerular capillary loops and decreased glomerular maturation index, as well as less CD31+ endothelium in cortical glomeruli at 12 dpp versus NS controls. Glomerular mesangial injury and glomerulosclerosis along with proteinuria were noted in PAN-injected kidneys starting from 30 dpp. Systolic blood pressure was increased significantly by 60 dpp in PAN mice. PAN mice also had significantly decreased Flk-1 and Tie2 mRNA expression and increased angiopoitein-1 (Ang-1) expression, without change in vascular endothelial growth factor (VEGF) at 2 dpp versus NS. Our study shows that podocyte injury in neonatal mice kidneys alters the expression of key capillary growth modulators in glomeruli, leading to abnormal development of glomerular capillaries, with subsequent development of proteinuria, hypertension, and glomerulosclerosis.
journal_name
Pediatr Resjournal_title
Pediatric researchauthors
Ma J,Rossini M,Yang HC,Zuo Y,Fogo AB,Ichikawa Idoi
10.1203/PDR.0b013e31813cbed1subject
Has Abstractpub_date
2007-10-01 00:00:00pages
417-21issue
4eissn
0031-3998issn
1530-0447pii
00006450-200710000-00007journal_volume
62pub_type
杂志文章abstract::Evidence is increasing for a role of polymorphisms in maternal or fetal innate immune response genes in preterm birth. Toll-like receptors (TLRs) are important receptors in the innate immunity. The genotype distribution of two TLR2 single nucleotide polymorphisms (SNPs) and one TLR4 SNP were determined among 524 neona...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e31813c9401
更新日期:2007-10-01 00:00:00
abstract::Soft bedding has been shown in epidemiologic studies to increase the risk for sudden, unexpected death in prone-sleeping infants. We compared the physical properties of conventional bedding to bedding from two sources: 1) bedding that covered the airways of victims of sudden infant death syndrome (SIDS) lying prone an...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199407001-00002
更新日期:1994-07-01 00:00:00
abstract::Near-infrared spectroscopy is a noninvasive method for monitoring brain oxygenation. The aim of the study was to investigate differences between cerebral oxygenation in different brain regions in newborns. In a prospective study, we monitored simultaneously left and right frontoparietal and temporo-occipital regional ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e31822a36db
更新日期:2011-10-01 00:00:00
abstract::In vitro experiments were performed to examine the contractile responsiveness of guinea pig gallbladder smooth muscle as a function of developmental age. Gallbladder muscle strips from preterm (day 50-55 gestation), newborn (days 1-3 post partum), and 1-month-old animals were stimulated with agonists that initiate the...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198603000-00003
更新日期:1986-03-01 00:00:00
abstract::Placental steroid sulfatase deficiency is a genetic disorder only recently reported in the medical literature. Most documented cases of placental sulfatase deficiency have been marked by delay in onset of labor, lack of cervical dilatation, and relative refractoriness of oxytocic agents and amniotomy. We have studied ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-197708000-00008
更新日期:1977-08-01 00:00:00
abstract::Long-term parenteral nutrition (TPN) in children is associated with sustained hyperinsulinemia due to a high nutriment infusion flow 12 h/24 h, with plausible lipotoxicity secondary to repeated lipid infusions and with changes in incretin hormone release. The aim of this study was to test whether long-term TPN can lea...
journal_title:Pediatric research
pub_type: 临床试验,杂志文章
doi:10.1203/PDR.0b013e3181559d5c
更新日期:2007-11-01 00:00:00
abstract:BACKGROUND:Heterozygous ATP-binding-cassette subfamily A member 3 (ABCA3) mutations are associated with neonatal respiratory complications. In an adult murine model, we investigated whether Abca3 haploinsufficiency is a predisposing factor for lung injury induced by hyperoxia or mechanical ventilation. METHODS:Abca3 h...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2013.127
更新日期:2013-10-01 00:00:00
abstract::Acute hemolytic anemia associated with red blood cell (RBC) glucose-6-phosphate dehydrogenase (G6PD) deficiency is commonly encountered in the Mediterranean basin. Nevertheless, concomitant clinical evidence of white blood cell G6PD deficiency is extremely rare in Israel. This study sought to assess simultaneously lev...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.PDR.0000120680.47846.47
更新日期:2004-05-01 00:00:00
abstract::In artificial tubes as well as in blood vessels with diameters less than 500 microns, blood viscosity decreases with decreasing diameter (Fahraeus-Lindqvist effect). Our study measured viscosity of red blood cells (RBC) from 10 preterm infants, 10 term neonates, and 10 adults by means of a capillary viscometer. RBC we...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198906000-00009
更新日期:1989-06-01 00:00:00
abstract:BACKGROUND:The pathogenesis of BPD includes inflammation and oxidative stress in the immature lung. Corticosteroids improve respiratory status and outcome, but the optimal treatment regimen for benefit with low systemic effects is uncertain. METHODS:In a pilot dose escalation trial, we administered ≤5 daily doses of b...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/s41390-020-01343-z
更新日期:2021-01-19 00:00:00
abstract::Young adult macrosomic offspring of streptozotocin-induced mildly hyperglycemic rats exhibit accelerated growth through the first 10 wk of age. At 10 wk, oral glucose loading resulted in elevated plasma insulin and glucose concentrations compared to controls. To assess the mechanism of the abnormal glucose tolerance i...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199107000-00008
更新日期:1991-07-01 00:00:00
abstract::To elucidate the impact of nutrition in cystic fibrosis (CF), we compared the phenotypic traits of Cftr -/- mice fed either a lipid-enriched liquid diet (Peptamen) or a standard chow combined with polyethylenglycol osmotic laxative (PEG), two strategies commonly used to prevent intestinal obstruction in CF mice. Survi...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e318155a61d
更新日期:2007-11-01 00:00:00
abstract::Leptin, a hormone secreted by adipocytes, is elevated in blood of obese adults. It is unknown whether the concentration is affected by gender, ethnicity, age, or stage of sexual maturation in children. We measured serum leptin levels in 183 children and 27 young adults using a double-antibody ELISA assay. Body fat mas...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199710000-00010
更新日期:1997-10-01 00:00:00
abstract::Bilirubin-IX alpha photooxidation products were detected by high performance liquid chromatography in the urine of neonates undergoing phototherapy for hyperbilirubinemia. The in vivo photoproducts were identified by chromatographic comparison with authentic synthetic standards using two complementary methods. Bilirub...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198408000-00003
更新日期:1984-08-01 00:00:00
abstract::Delivery of M. nemestrina at 80% of normal gestation provides a population of neonates at high risk for hyaline membrane disease (HMD). The diagnosis of HMD was made by the presence of reticulogranular densities and air bronchograms on chest radiographs. Patchy atelectasis was seen in the lungs of animals assigned by ...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-197905000-00017
更新日期:1979-05-01 00:00:00
abstract::The microbiome, of which the bacterial component alone (microbiota), is estimated to include 10 times more cells than human cells of the body, blooms immediately after birth and evolves in composition and complexity throughout childhood. The gut microbiome has a profound impact on gastrointestinal tract development, m...
journal_title:Pediatric research
pub_type: 杂志文章,评审
doi:10.1038/pr.2014.162
更新日期:2015-01-01 00:00:00
abstract::Although cisapride is widely used to treat gastrointestinal motility disorders, it has been associated with QT prolongation, torsades de pointes, and cardiac arrest. Only in children, however, has atrioventricular (AV) block after cisapride been reported. This study used Langendorff perfusion to determine the direct e...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.PDR.0000050122.67091.1D
更新日期:2003-03-01 00:00:00
abstract::A double-label, double-substrate incubation technique has been developed and used to study the conversion of progesterone to testosterone in testes extracts from incompletely virilized males. The procedure involves separation of the microsomes from a testicular homogenate, incubating the microsomes with 1 microM [7-3H...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198408000-00018
更新日期:1984-08-01 00:00:00
abstract::Cerebral concentrations of phenylalanine (PHE) were measured by means of quantitative in vivo 1H MR spectroscopy in 8 adult patients treated early for phenylketonuria type I. A 1.5-Tesla routine magnetic resonance scanner, localization sequence with short echo time (20 ms), and a fully automated data processing scheme...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199511000-00005
更新日期:1995-11-01 00:00:00
abstract::Birth is characterized by swift and complex transitions in hemodynamic and respiratory variables. Unrecognized pathologies or incidents may quickly become fatal or cause permanent damage. This article introduces an essential component of an acute perinatal care simulator, namely a model for educational simulation of n...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e3181c2def3
更新日期:2010-02-01 00:00:00
abstract::Recent studies have shown 1,25(OH)2D3 receptor-mediated modulation of leukocyte proliferation, differentiation, and function. We examined the phagocytosis and killing of microorganisms by neutrophils and monocytes from five patients of three families with hereditary resistance to 1,25(OH)2D3. Phagocytosis of microorga...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198903000-00012
更新日期:1989-03-01 00:00:00
abstract::Nitric oxide (NO) serves many vasoprotective roles, but the massive release of NO causes arterial wall degeneration. We investigated whether enhanced nitric oxide synthase (iNOS) expression in peripheral blood leukocytes and circulating endothelial cells mirrors the progression of coronary arterial lesions in 55 child...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/01.PDR.0000113464.93042.A4
更新日期:2004-04-01 00:00:00
abstract::Long chain polyunsaturated fatty acids (LCPUFA)seem to be the most trophic macronutrients in inducing intestinal adaptation in adult short bowel syndrome (SBS), although their effects on intestinal adaptation in infants with SBS remain unknown.It is hypothesized that a high fat diet enriched with n-3 LCPUFA derived fr...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/PDR.0b013e3181eb2ee5
更新日期:2010-09-01 00:00:00
abstract::A 10-year longitudinal study was conducted on 16 prepubescent youngsters who had undergone successful weight reduction. Their ages ranged from 2 to 10 years when the study began. In all subjects, weight reduction proceeded only by a decrease in adipose cell size (from 0.62 +/- 0.02 to 0.46 +/- 0.02 micrograms lipid pe...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198110000-00016
更新日期:1981-10-01 00:00:00
abstract::The present study aimed to test whether an acute period of asphyxia during birth in the rat results in long-term alterations in CNS function. Morphologic studies have indicated that the hippocampus is particularly vulnerable to perinatal anoxia. Thus, the present study tested adult rats, which had undergone acute birt...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199504000-00018
更新日期:1995-04-01 00:00:00
abstract:BACKGROUND:Necrotizing enterocolitis is characterized by intestinal inflammation and epithelial barrier dysfunction. Mitogen-activated protein kinase (MAPK) phosphatase (MKP)-1 plays a pivotal role in the feedback control of MAPK signaling, which regulates inflammation and apoptosis. We hypothesized that MKP-1 prevents...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1038/pr.2015.88
更新日期:2015-08-01 00:00:00
abstract::Cerebral magnetic resonance imaging (MRI) has revealed white matter changes in patients with phenylketonuria (PKU), an inborn error of metabolism with increased plasma phenylalanine level. Because the significance of these lesions is unknown, this study was undertaken to determine whether glucose metabolism was depres...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199607000-00004
更新日期:1996-07-01 00:00:00
abstract::We measured the changes in tidal volume, duration of the various phases of the respiratory cycle, and peak nasal pressure during elastic and resistive loading in preterm infants. Values were calculated during the first loaded breath, when chemical drive was unchanged. Tidal volume decreased by equivalent percentages w...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-197704000-00002
更新日期:1977-04-01 00:00:00
abstract::Prenatal maternal therapy with glucocorticoid reduces the incidence of respiratory distress syndrome (RDS) in premature infants. To investigate the effects of this treatment on the fetal endocrine system, we determined serum concentrations of betamethasone, cortisol, dehydroepiandrosterone sulfate, growth hormone, and...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198002000-00011
更新日期:1980-02-01 00:00:00
abstract::We studied properties of saliva and of dental plaque which affect the caries process in an effort to understand the low prevalence of caries in patients with chronic renal failure. Plaque pH, before and following carbohydrate exposure, saliva pH, and saliva composition were evaluated in children and adolescents with c...
journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198508000-00003
更新日期:1985-08-01 00:00:00