Abstract：
:Exosomes are small membrane vesicles that are secreted from a variety of cell types into various body fluids including the blood and urine. These vesicles are thought to play a role in cell-cell interactions. CD24 is a small but extensively glycosylated protein linked to the cell surface by means of a glycosyl-phosphatidylinositol anchor. In this study we found that CD24 is present in membrane vesicles characterized as exosomes that were isolated from the urine of normal individuals. CD24 was expressed by both tubule cells and podocytes and treatment of the latter with a cholesterol-extracting agent, but not with a calcium ionophore, caused the release of CD24-containing exosomes. Using CD24 as a marker, we found exosomes in the urine of newborn infants and in the amniotic fluid of pregnant women with similar findings made in mice. Interestingly, studies with CD24 knockout mice showed that the exosomes are released from the fetus but not from the mother; however, exosome release was similar from both the knockout and the wild-type mice. This indicates that CD24 is not essential for exosome formation or release but may be a convenient exosome marker. Our studies suggest that exosomal secretion from the embryonic kidney could play a biological role at the fetal-maternal interphase.
journal_name
Kidney Intjournal_title
Kidney internationalauthors
Keller S,Rupp C,Stoeck A,Runz S,Fogel M,Lugert S,Hager HD,Abdel-Bakky MS,Gutwein P,Altevogt Pdoi
10.1038/sj.ki.5002486subject
Has Abstractpub_date
2007-11-01 00:00:00pages
1095-102issue
9eissn
0085-2538issn
1523-1755pii
S0085-2538(15)52799-3journal_volume
72pub_type
杂志文章abstract：:Glomerular podocytes are terminally differentiated cells, and podocyte loss is a common feature of progressive renal pathologies. In this issue, Romoli et al. focus on podocyte progenitors that were proposed to reside in the Bowman's capsule. They demonstrate that suppression of CXCL12/CXCR4 signaling activates pariet...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1016/j.kint.2018.09.011
更新日期：2018-12-01 00:00:00
abstract：:The binding of albumin to the glomerular capillary wall was studied using albumin-gold in perfused kidneys, the interaction of [3H]albumin with isolated glomeruli at 37 degrees C and 4 degrees C and the interaction at [3H]albumin with purified basement membrane. The albumin-gold was found to bind predominantly to the ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1995.149
更新日期：1995-04-01 00:00:00
abstract：BACKGROUND:Wegener's granulomatosis (WG) is characterized by systemic vasculitis with crescentic glomerulonephritis (CGN) and circulating autoantibodies directed against neutrophil cytoplasmic antigens (ANCA). Proteinase 3 (PR-3), a neutral serine proteinase in neutrophils implicated in the growth control of myeloid ce...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2000.00100.x
更新日期：2000-06-01 00:00:00
abstract：:Hemodialysis adequacy can be quantified using ultraviolet absorbance of the spent dialysate, or by analysis of dialysate conductivity at the dialyzer inlet and outlet in response to changes in dialysate electrolyte concentration. These measurements can be made at every dialysis, including initial and acute treatments ...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1038/ki.2010.218
更新日期：2010-11-01 00:00:00
abstract：BACKGROUND:Angiotensin II (Ang II) can up-regulate nicotinamide adenine dinucleotide phosphate [NAD(P)H] oxidase, whose product superoxide anion (O2-) can interact with nitric oxide (NO) to form peroxynitrite (ONOO-). We tested the hypothesis that Ang II subtype 1 (AT1) receptor activation enhances oxidative stress and...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2002.00123.x
更新日期：2002-01-01 00:00:00
abstract：:Macrophages are inflammatory cells with important roles in the propagation of renal injury. More recently they have also been shown to be important in the resolution of inflammation. Wang et al. show that macrophages can be modulated ex vivo by cytokine stimulation, localize to renal tissue, and slow progression of ex...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1038/sj.ki.5002332
更新日期：2007-08-01 00:00:00
abstract：:We evaluated the effects of the bisphosphonate pamidronate on bone histomorphometry, structure and strength in male rats with uninephrectomy or with chronic renal disease induced by 5/6 nephrectomy. In rats with chronic renal disease the plasma urea, phosphate and parathyroid hormone levels were significantly increase...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2008.180
更新日期：2008-08-01 00:00:00
abstract：:Medial arterial calcification involves chondrogenic/osteogenic transdifferentiation of vascular smooth muscle cells (VSMCs). Zhao et al. report that phosphate activates the mammalian target of rapamycin (mTOR) cascade in VSMCs, leading to downregulation of Klotho. Furthermore, rapamycin was shown to halt medial calcif...
journal_title：Kidney international
pub_type： 评论,杂志文章
doi：10.1038/ki.2015.223
更新日期：2015-10-01 00:00:00
abstract：:These studies were undertaken to examine effects of elevated glucose levels on glycolysis, sorbitol pathway activity, and the cytosolic redox state of NADH/NAD+ in isolated glomeruli. Blood-free glomeruli were isolated from kidneys of male, Sprague-Dawley rats using standard sieving techniques, then incubated for one ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1992.121
更新日期：1992-04-01 00:00:00
abstract：:The monitoring of the levels of alloantibodies following transplantation might facilitate early diagnosis of chronic rejection (CR), the leading cause of renal allograft failure. Here, we used serial alloantibody surveillance to monitor patients with preoperative positive flow cytometric crossmatch (FCXM). Sixty-nine ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2010.556
更新日期：2011-05-01 00:00:00
abstract：BACKGROUND:Tacrolimus has a narrow therapeutic window, and bioavailability is known to vary considerably between renal transplant recipients. Most centers still rely on measurement of trough levels, but there are conflicting reports on the correlation between tacrolimus trough levels and systemic exposure, as measured ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1111/j.1523-1755.2005.00352.x
更新日期：2005-06-01 00:00:00
abstract：:The renal proximal tubule actively transports charged, potentially toxic xenobiotics from blood to lumen. Basolateral uptake of organic anions is indirectly coupled to the sodium gradient through Na-dicarboxylate cotransport and dicarboxylate-organic anion exchange. Upon entry, a significant fraction of intracellular ...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1038/ki.1996.240
更新日期：1996-06-01 00:00:00
abstract：BACKGROUND:Both high glucose (HG) and angiotensin II (Ang II) causes glomerular mesangial cell (GMC) growth and increased synthesis of matrix proteins like collagen IV contributing to diabetic nephropathy. We have recently found that exposure of vascular smooth muscle cells to HG augments the Ang II activation of the g...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2002.00311.x
更新日期：2002-05-01 00:00:00
abstract：:The treatment of hyperphosphatemia remains challenging in patients receiving hemodialysis. This Phase 1b study assessed safety and efficacy of EOS789, a novel pan-inhibitor of phosphate transport (NaPi-2b, PiT-1, PiT-2) on intestinal phosphate absorption in patients receiving intermittent hemodialysis therapy. Two cro...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1016/j.kint.2020.09.035
更新日期：2020-10-30 00:00:00
abstract：:The molecular mechanism of Na+ and phosphate transport by the intestinal brush border membrane Na+/phosphate cotransporter was examined using anion and cation exchange columns interfaced to a light-activated microsecond timer. The nature of ion:cotransporter interaction was examined for sensitivity to itself versus no...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1996.141
更新日期：1996-04-01 00:00:00
abstract：:Meprins are zinc-dependent metalloproteinases that are highly expressed in the brush-border membranes of both the kidney and the intestines. Meprins are capable of proteolytically degrading extracellular matrix proteins, proteolytically processing bioactive proteins, and play a role in inflammatory processes. In this ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/sj.ki.5002189
更新日期：2007-05-01 00:00:00
abstract：:Studies were undertaken to examine the mechanism whereby changes in intraluminal flow rates after reabsorption in the isolated perfused proximal tubule of the rabbit. All protocols employed the technique of in vitro perfusion of isolated segments of the proximal convoluted tubule. Stepwise elimination of d-glucose and...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1977.3
更新日期：1977-01-01 00:00:00
abstract：:Undecalcified sections of doubly tetracycline-labeled transiliac bone biopsy specimens obtained from ten hemodialyzed patients before and 10 to 16 months after parathyroidectomy (PTX) were analyzed. Before parathyroidectomy (total PTX with autotransplant in six patients and subtotal PTX in four patients), all the pati...
journal_title：Kidney international
pub_type： 临床试验,杂志文章
doi：10.1038/ki.1985.27
更新日期：1985-02-01 00:00:00
abstract：BACKGROUND:Strict glycemic control and antihypertensive treatment may decrease but not eliminate the risk of progressive nephropathy in diabetic patients. C-peptide has been shown to exert beneficial effects on complications, including incipient nephropathy, in type 1 diabetes. METHODS:Renal effects of 14 days of intr...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2001.00964.x
更新日期：2001-10-01 00:00:00
abstract：:Insulin resistance has been associated with kidney disease even in the absence of diabetes; however, pathways linking insulin resistance to kidney disease are unclear. The purpose of this study was to determine if transforming growth factor (TGF)-beta1, a key cytokine associated with kidney disease, responds to circul...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2010.109
更新日期：2010-08-01 00:00:00
abstract：BACKGROUND:Mast cells are growth factor-rich, bone marrow-derived cells that infiltrate injured tissue where they have been implicated in the pathogenesis of progressive fibrosis. METHODS:Mast cell infiltration and the expression of related chemoattractants was examined following 5/6 nephrectomy, a model of progressiv...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2003.00183.x
更新日期：2003-09-01 00:00:00
abstract：:Administration of antibody, directed against glomerular basement membrane (GBM) heparan sulfate-proteoglycan, into a presensitized rat results in the induction of membranous nephropathy with subepithelial immune-complex deposits. In this investigation, we examined the mechanisms responsible for the formation of subepi...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1988.166
更新日期：1988-08-01 00:00:00
abstract：BACKGROUND:Renal failure and toxic acute tubular necrosis (ATN) may be seen following exposure to a variety of therapeutic agents. Zoledronate (Zometa) is a new, highly potent bisphosphonate used in the treatment of hypercalcemia of malignancy. We report the first clinical-pathologic study of nephrotoxicity associated ...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2003.00071.x
更新日期：2003-07-01 00:00:00
abstract：INTRODUCTION:Transforming growth factor-beta (TGF-beta) is known to play a pivotal role in the regulation of extracellular matrix (ECM) accumulation. Since diabetic nephropathy (DMN) is characterized by basement membrane thickening and mesangial expansion, control of ECM deposition is believed to be important in the pa...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.2003.00919.x
更新日期：2003-05-01 00:00:00
abstract：:In uremic patients resistance to the action of insulin has been documented, but it is not known at what stage of renal disease it appears. We therefore examined 29 patients with IgA glomerulonephritis (IgAGN) and 21 patients with adult polycystic kidney disease (ADPKD) in different stages of renal failure, and in addi...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1046/j.1523-1755.1998.00898.x
更新日期：1998-05-01 00:00:00
abstract：:We reviewed the diagnostic features and clinical course of 140 patients with idiopathic membranous nephropathy who had their index renal biopsies performed at the Mayo Clinic between 1972 and 1984. There were 93 males and 47 females (average age, 50.8 +/- 17 years); 116 patients (83%) had the nephrotic syndrome and 42...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1988.56
更新日期：1988-03-01 00:00:00
abstract：:Polyomavirus-associated nephropathy (PVAN) is a significant complication after kidney transplantation, often leading to premature graft loss. In order to identify antiviral responses of the renal tubular epithelium, we studied activation of the viral DNA and the double-stranded RNA (dsRNA) sensors Toll-like receptor 3...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.2011.311
更新日期：2012-01-01 00:00:00
abstract：:Since the kidney medulla was reported to generate platelet activating factor (PAF), we investigated a possible effect of this agent on the reabsorptive function of in vitro microperfused medullary thick ascending limbs from mouse kidney (mTAL). PAF, 10(-7) M in the bath, significantly decreased the net chloride flux (...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1992.38
更新日期：1992-02-01 00:00:00
abstract：:Space medicine and new technology such as magnetic resonance imaging of tissue sodium stores (23NaMRI) have changed our understanding of human sodium homeostasis and pathophysiology. It has become evident that body sodium comprises 3 main components. Two compartments have been traditionally recognized, namely one that...
journal_title：Kidney international
pub_type： 杂志文章,评审
doi：10.1016/j.kint.2018.09.024
更新日期：2019-02-01 00:00:00
abstract：:The present experiments were performed to examine the effect of variation in protein intake on renal function and morphology in the non-clipped kidneys of Goldblatt hypertensive rats. After renal artery clipping, rats were put on diets containing 5 (LP), 17.5 (NP), or 51% (HP) protein. After 4 to 5 weeks, all rats had...
journal_title：Kidney international
pub_type： 杂志文章
doi：10.1038/ki.1986.30
更新日期：1986-02-01 00:00:00
