Simultaneous occlusion of Na+ and phosphate by the intestinal brush border membrane Na+/phosphate cotransporter.

Abstract:

:The molecular mechanism of Na+ and phosphate transport by the intestinal brush border membrane Na+/phosphate cotransporter was examined using anion and cation exchange columns interfaced to a light-activated microsecond timer. The nature of ion:cotransporter interaction was examined for sensitivity to itself versus non-substrates, and sensitivity to amino acid specific reagents. Two possible ion:cotransporter interactions were examined. (a) ion bound, where ion exchanges rapidly with medium ion but not non-substrates, and (b) ion occluded, where ion exchanges slowly with medium ion. The Na+/phosphate cotransporter was found to occlude Na+ or Na+ and H2PO4, but not H2PO4 alone or HPO4. Phosphate occlusion had an absolute requirement for Na+ with K+ or Cs+ unable to substitute. Phosphate occlusion distinguished between phosphate and sulfate. Deocclusion from the fully loaded cotransporter was consistent with the release of 2 Na+'s prior to phosphate. These results predict two ternary cotransporter conformations differing in the Na+ sensitivity of their phosphate affinity.

journal_name

Kidney Int

journal_title

Kidney international

authors

Peerce BE

doi

10.1038/ki.1996.141

subject

Has Abstract

pub_date

1996-04-01 00:00:00

pages

988-91

issue

4

eissn

0085-2538

issn

1523-1755

pii

S0085-2538(15)59435-0

journal_volume

49

pub_type

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