Abstract：
:In the microenvironment of solid growing tumors, pronounced hypoxia or extracellular acidosis is commonly found. The aim of this study was the analysis of the cytotoxic effect of different chemotherapeutic agents (cisplatin, daunorubicin, docetaxel) under these conditions in vitro. Prostate carcinoma cells (R3327-AT1) were exposed to hypoxia (pO2 < 0.5 mmHg) or extracellular acidosis (pH = 6.6) for 6h. After 3h, cytotoxic drugs were added. The cytotoxic effect was assessed by measuring caspase 3-activity (apoptosis), LDH release (necrosis) and repopulation of the cells after chemotherapy (cell death). Compared to aerobic control conditions, severe hypoxia over 6 h per se led to a slight increase in apoptosis, necrosis and cell death. With all three chemotherapeutic agents, hypoxia led to a reduced (by approx. 25%) caspase 3-activity and a marked increase in necrosis. However, the overall cytotoxicity of the drug was not affected by O2-deficiency. By contrast, during extracellular acidosis, the cytotoxic effect of daunorubicin was reduced by 40%, preferentially due to a marked reduction in apoptosis. With cisplatin and docetaxel no change in overall cell death was detected. However, for daunorubicin the tumor-pH seems to have a strong impact on cytotoxicity. With this chemotherapeutic drug the therapeutic efficacy is markedly reduced in an acidotic environment.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Thews O,Gassner B,Kelleher DK,Schwerdt G,Gekle Mdoi
10.1007/978-0-387-71764-7_21subject
Has Abstractpub_date
2007-01-01 00:00:00pages
155-61eissn
0065-2598issn
2214-8019journal_volume
599pub_type
杂志文章abstract：:The availability of numerical data grows from 1 day to another in a remarkable way. New technologies of high-throughput Next-Generation Sequencing (NGS) are producing DNA sequences. Next-Generation Sequencing describes a DNA sequencing technology which has revolutionized genomic research. In this paper, we perform som...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-3-030-32622-7_42
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abstract：:In order to clarify if "idiopathic" RPGN still exists as a distinct entity we reviewed 41 patients with histological picture of diffuse crescentic GN (60% of crescents) and no clinical evidence of systemic disease. According to the presence or absence of intraglomerular necrotizing lesions we subdivided the patients i...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4757-9182-2_76
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abstract：:It is clear that the combination of 31P NMR studies of muscle and of other organs with biochemical and physiological measurements is an extremely powerful method for studying cellular energetics. We have presented examples of our on-going experiments. Well defined and stable in vitro preparations of mammalian fast-twi...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4684-7790-0_27
更新日期：1983-01-01 00:00:00
abstract：:Terminal Transferase (TdT), Adenosine Deaminase (ADA), immunological membrane markers, cytochemical reactivity and cytogenetics were analyzed in 226 patients with ALL, AUL and AML, in 70 patients with CML and in 3 cases of Ph' positive acute leukemia presenting as ALL. TdT was tested in peripheral blood and bone marro...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4684-8929-3_24
更新日期：1982-01-01 00:00:00
abstract：:Extensive use of antibiotics in both public health and animal husbandry has resulted in rapid emergence of antibiotic resistance in almost all human pathogens, including biothreat pathogens. Antibiotic resistance has thus become a major concern for both public health and national security. We developed multiplexed ass...
journal_title：Advances in experimental medicine and biology
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doi：10.1007/978-1-4419-5913-3_51
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abstract：:The influence of bovine lactoferrin (bLf) on colon carcinogenesis was investigated in male F344 rats treated with azoxymethane (AOM). In experiment I, 2% and 0.2% bLf, and Bifidobacterium longum (B. longum) as a positive control at 3% were given in the diet for 4 weeks, along with two s.c. 15 mg/kg injections of AOM o...
journal_title：Advances in experimental medicine and biology
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abstract：:Immunization of strain 2 guinea pigs with 10(7) syngeneic Ia+ L2C leukemia cells in adjuvant leads to L2C tumor protection. After subsequent challenges with L2C tumor cells, the sera and spleens of the protected guinea pigs had detectable anti-L2C reactivity. The L2C antibody reactivity was preferentially associated w...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4757-1410-4_7
更新日期：1983-01-01 00:00:00
abstract：:Embryo implantation requires a reciprocal interaction between the blastocyst and endometrium and is associated with complex regulatory mechanisms. Since their discovery, microRNAs became prominent candidates providing missing links for many biological pathways. In recent years, microRNAs were implicated as one of the ...
journal_title：Advances in experimental medicine and biology
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doi：10.1007/978-3-319-22380-3_8
更新日期：2015-01-01 00:00:00
abstract：:Bronchitis is a common health problem in children. Frequent bronchitis in infancy increases the risk of developing chronic respiratory diseases. The aim of the study was to assess the level of growth and the nutritional status in children and youths with special regard to the level of body fatness assessed by measurin...
journal_title：Advances in experimental medicine and biology
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doi：10.1007/5584_2015_192
更新日期：2016-01-01 00:00:00
abstract：:Feedback from muscle afferents is essential for locomotion to be functional under changing external conditions. In this article, we review the role of afferent feedback in adapting locomotor activity to transient and more sustained changes in sensory input in reduced and walking cat preparations. Much of the work on m...
journal_title：Advances in experimental medicine and biology
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doi：10.1007/978-1-4615-0713-0_40
更新日期：2002-01-01 00:00:00
abstract：:The rates of ethanol oxidation in various species are linked to the rates of general metabolism, and more specifically that of O2 utilization by the liver. After chronic ethanol administration, increased oxidation of ethanol is accompanied by increased total hepatic O2 utilization, as studied in the whole animal, the ...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4899-5181-6_22
更新日期：1977-01-01 00:00:00
abstract：:Renewal of rod photoreceptor outer segments in the mammalian eye involves synchronized diurnal shedding after light onset of spent distal outer segment fragments (POS) linked to swift clearance of shed POS from the subretinal space by the adjacent retinal pigment epithelium (RPE). Engulfed POS phagosomes in RPE cells ...
journal_title：Advances in experimental medicine and biology
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更新日期：2016-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 临床试验,杂志文章
doi：10.1007/978-0-387-85998-9_39
更新日期：2009-01-01 00:00:00
abstract：:Mutations in the gene Crumbs homolog 1 (CRB1) are responsible for several retinopathies that are diverse in severity and phenotype. Thus, there is considerable incentive to determine how disruption of this gene causes disease. Progress on this front will aid in developing molecular diagnostics that can predict disease...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-030-27378-1_41
更新日期：2019-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-319-75402-4_10
更新日期：2018-01-01 00:00:00
abstract：:The microbiome comprises all the genetic material within a microbiota, that represents tenfold higher than that of our cells. The microbiota it includes a wide variety of microorganisms such as bacteria, viruses, protozoans, fungi, and archaea, and this ecosystem is personalized in any body space of every individual. ...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-030-24100-1_10
更新日期：2019-01-01 00:00:00
abstract：:The Saccharomyces cerevisiae branchpoint binding protein (BBP) is a 53 kDa pre-mRNA processing factor with characteristic STAR/GSG protein organization. This includes a central RNA binding site composed of an extended Type I KH domain with an adjacent QUA2 motif. Downstream of KH-QUA2 are two CCHC-type zinc knuckles a...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：
更新日期：2010-01-01 00:00:00
abstract：:To assess whether the dipeptide N-epsilon-(gamma-L-glutamyl)-L-lysine (glutamyl-lysine) can serve as a nutritional source of lysine, we compared the growth of mice fed (a) an amino acid diet in which lysine was replaced by six dietary levels of glutamyl-lysine; (b) wheat gluten diets fortified with lysine; (c) a wheat...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4899-2626-5_30
更新日期：1991-01-01 00:00:00
abstract：:These investigations were undertaken to elucidate whether the difference between the time courses of the isometric tension and the velocity of shortening upon releases to a given isotonic load various times during a twitch is due to a change in the kinetics of the individual cross-bridges, i.e. in the rate constants g...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：:Cytochrome P450 enzymes (P450s or CYPs) catalyze an enormous variety of oxidative reactions in organisms from all major domains of life. Their monooxygenase activity relies on the reductive scission of molecular oxygen (O2) bound to P450 heme iron, and thus on the delivery of two electrons to the heme iron at discrete...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-319-16009-2_11
更新日期：2015-01-01 00:00:00
abstract：:Biomembranes separate a live cell from its environment and keep it in an off-equilibrium, steady state. They contain both phospholipids and nonphospholipids, depending on whether there are phosphate groups in the headgroup regions. Cholesterol (CHOL) is one type of nonphospholipids, and one of the most abundant lipid ...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-030-04278-3_8
更新日期：2019-01-01 00:00:00
abstract：:Cancer is considered the disease of the century, which can be easily understood considering its increasing incidence worldwide. Over the last years, nanotechnology has been presenting promising theranostic approaches to tackle cancer, as the development of nanoparticle-based therapies. But, regardless of the promising...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-030-36588-2_10
更新日期：2020-01-01 00:00:00
abstract：:Cyanobacteria are autotrophic prokaryotes that can perform oxygenic photosynthesis. The conversion of light and carbon dioxide into green fuels and chemicals has drawn considerable interest, and several dozen products have been successfully synthesized in genetically engineered cyanobacteria. However, during cultivati...
journal_title：Advances in experimental medicine and biology
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更新日期：2018-01-01 00:00:00
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更新日期：2018-01-01 00:00:00
abstract：:The Langmuir trough approach to the study of the physical properties of an in vitro system representing the natural tear lipid-aqueous interface gives useful information on the effect produced by changes of composition of both phases. We found that variations of the composition of the lipid mixture affect more strongl...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4615-0717-8_58
更新日期：2002-01-01 00:00:00
abstract：:Macromolecules have been prepared containing native insulin carried by a modified insulin skeleton made by partially thiolating the insulin hexamer and forming intermolecular cross-links through disulphide bridges. Oxidation of partially thiolated insulin (0.5-0.7 SH group/mole), formed by reacting insulin with AHTL, ...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4684-3282-4_15
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abstract：:Asthma is a heterogeneous inflammatory disease. Most patients respond to current standard of care, i.e., bronchodilators, inhaled glucocorticosteroids and other anti-inflammatory drugs, but in some adequate asthma control cannot be achieved with standard treatments. These difficult-to-treat patients would be the targe...
journal_title：Advances in experimental medicine and biology
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更新日期：2016-01-01 00:00:00
abstract：:Clostridium difficile infection (CDI) is one of the most common healthcare-associated infections in the world and is a leading cause of morbidity and mortality in hospitalized patients.Although several antibiotics effectively treat CDI, some individuals do not respond to these drugs and may be cured by transplanting s...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-319-72799-8_11
更新日期：2018-01-01 00:00:00
abstract：:An integrated left ventricular (LV) model is used to solve, simultaneously and interactively, the LV mechanics, the coronary blood flow and capillary and interstitial fluid and mass transport, and to analyze the LV behavior under normal as well as pathological conditions. Accounting for the interstitial fluid mass bal...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4615-2946-0_21
更新日期：1993-01-01 00:00:00
abstract：:The human Usher syndrome (USH) is the most frequent cause of combined hereditary deaf-blindness. USH is genetically and clinically heterogeneous: 15 chromosomal loci assigned to 3 clinical types, USH1-3. All USH1 and 2 proteins are organized into protein networks by the scaffold proteins harmonin (USH1C), whirlin (USH...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4614-3209-8_67
更新日期：2014-01-01 00:00:00
