Abstract：
:These investigations were undertaken to elucidate whether the difference between the time courses of the isometric tension and the velocity of shortening upon releases to a given isotonic load various times during a twitch is due to a change in the kinetics of the individual cross-bridges, i.e. in the rate constants governing the transitions between the various strongly bound cross-bridge states. Single skeletal muscle fibres from the frog (Rana temporaria, sarcomere length 2.3 microns, temperature 4-6 degrees C) were stimulated to produce isometric twitches and released after various delays to shorten isotonically against finite loads (P). Force, stiffness (K, measured by longitudinal oscillation 0.3-0.8 nm per half-sarcomere peak-to-peak, 2 kHz), and change in length were measured simultaneously. The velocity of shortening (V) was estimated as the quasi steady-state value, i.e. the value attained after the initial oscillatory transient response to the change in length preceding the isotonic shortening. A reduction in P led to a reduction in K, however, the relative reduction in K was not as great as the relative reduction in P. Thus, when extrapolated to P = O, K was about one third of its peak isometric value. Under the assumption that K is to the first order of approximation proportional to the number of strongly bound cross-bridges, P was divided by K to give an estimate of the average load per cross-bridge yo = P/K.V showed an almost linear dependence on yo (correlation coefficients 0.95-0.998). The slope and intercept of this relation (regression analysis) showed no dependence on the delay after stimulation of the release to the isotonic load. The results can be explained if the kinetic properties of the individual strongly bound cross-bridges are unaffected by the level of activation which changes during the course of the contraction.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Haugen Psubject
Has Abstractpub_date
1988-01-01 00:00:00pages
461-71eissn
0065-2598issn
2214-8019journal_volume
226pub_type
杂志文章abstract：:Although controversial, animal and tissue studies indicate that carotid bodies are sensitive to changes in glucose as well as in oxygen, thereby functioning as metabolic sensors. This study was designed to test the hypothesis that carotid bodies in humans participate in the counter-regulatory response to insulin-induc...
journal_title：Advances in experimental medicine and biology
pub_type： 临床试验,杂志文章
doi：10.1007/978-90-481-2259-2_31
更新日期：2009-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
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pub_type： 杂志文章,评审
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更新日期：2018-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
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更新日期：1981-01-01 00:00:00
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pub_type： 杂志文章
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
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更新日期：1975-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
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更新日期：2016-01-01 00:00:00
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更新日期：2012-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
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更新日期：2019-01-01 00:00:00
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更新日期：2016-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
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更新日期：2019-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-0-387-09794-7_3
更新日期：2008-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4419-0296-2_4
更新日期：2009-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4615-9376-8_20
更新日期：1984-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：
更新日期：1979-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4613-1927-6_15
更新日期：1987-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4684-3291-6_17
更新日期：1985-01-01 00:00:00
abstract：:The cytoskeleton consists of filamentous protein polymers that form organized structures, contributing to a multitude of cell life aspects. It includes three types of polymers: the actin microfilaments, the microtubules and the intermediate filaments. Decades of research have implicated the cytoskeleton in processes t...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-030-25650-0_12
更新日期：2019-01-01 00:00:00
