Abstract:
BACKGROUND:Epidermal growth factor receptor inhibitor therapy is now approved for treatment of metastatic colorectal carcinomas (CRC) in patients with tumors lacking KRAS mutations. Several procedures to detect KRAS mutations have been developed. However, the analytical sensitivity and specificity of these assays on routine clinical samples are not yet fully characterised. METHODS:The practical aspects and clinical applicability of a KRAS-assay based on Pyrosequencing were evaluated in a series of 314 consecutive CRC cases submitted for diagnostic KRAS analysis. The performance of Pyrosequencing compared to allele-specific, real-time PCR was then explored by a direct comparison of CE-IVD-marked versions of Pyrosequencing and TheraScreen (DxS) KRAS assays for a consecutive subset (n = 100) of the 314 clinical CRC samples. RESULTS:Using Pyrosequencing, 39% of the 314 CRC samples were found KRAS-mutated and several of the mutations (8%) were located in codon 61. To explore the analytical sensitivity of the Pyrosequencing assay, mutated patient DNA was serially diluted with wild-type patient DNA. Dilutions corresponding to 1.25-2.5% tumor cells still revealed detectable mutation signals. In clinical practice, our algorithm for KRAS analysis includes a reanalysis of samples with low tumor cell content (< 10%, n = 56) using an independent assay (allele-specific PCR, DxS). All mutations identified by Pyrosequencing were then confirmed and, in addition, one more mutated sample was identified in this subset of 56 samples. Finally, a direct comparison of the two technologies was done by re-analysis of a subset (n = 100) of the clinical samples using CE-IVD-marked versions of Pyrosequencing and TheraScreen KRAS assays in a single blinded fashion. The number of samples for which the KRAS codon 12/13 mutation status could be defined using the Pyrosequencing or the TheraScreen assay was 94 and 91, respectively, and both assays detected the same number of codon 12 and 13 mutations. CONCLUSIONS:KRAS mutation detection using Pyrosequencing was evaluated on a consecutive set of clinical CRC samples. Pyrosequencing provided sufficient analytical sensitivity and specificity to assess the mutation status in routine formalin-fixed CRC samples, even in tissues with a low tumor cell content.
journal_name
BMC Cancerjournal_title
BMC cancerauthors
Sundström M,Edlund K,Lindell M,Glimelius B,Birgisson H,Micke P,Botling Jdoi
10.1186/1471-2407-10-660subject
Has Abstractpub_date
2010-12-01 00:00:00pages
660issn
1471-2407pii
1471-2407-10-660journal_volume
10pub_type
杂志文章相关文献
BMC CANCER文献大全abstract:BACKGROUND:Homeobox B4 (HOXB4) is correlated with poor prognosis of various cancer types. However, how HOXB4 promotes ovarian cancer (OV) progression remains unclear. METHODS:The Cancer Genome Atlas (TCGA) database indicated that a high level of HOXB4 in OV was correlated with poor prognosis. The biological functions ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-020-06725-4
更新日期:2020-03-16 00:00:00
abstract:BACKGROUND:An imbalance between proliferation and apoptosis is one of the main features of carcinogenesis. TRAIL (TNF-related apoptosis-inducing ligand) induces apoptosis upon binding to the TRAIL death receptors, TRAIL receptor 1 (TRAIL-R1) and TRAIL-R2, whereas binding to TRAIL-R3 and TRAIL-R4 might promote cell surv...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-13-532
更新日期:2013-11-09 00:00:00
abstract:BACKGROUND:Oncolytic viruses, including vaccinia virus (VACV), are a promising alternative to classical mono-cancer treatment methods such as surgery, chemo- or radiotherapy. However, combined therapeutic modalities may be more effective than mono-therapies. In this study, we enhanced the effectiveness of oncolytic vir...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-12-366
更新日期:2012-08-23 00:00:00
abstract:BACKGROUND:Pancreatic ductal adenocarcinoma (PDAC) is considered as one of the most aggressive cancers lacking efficient early detection biomarkers. Circulating miRNAs are now being considered to have potency to be used as diagnostic and prognostic biomarkers in different diseases as well as cancers. In case of cancer,...
journal_title:BMC cancer
pub_type: 杂志文章,meta分析
doi:10.1186/s12885-019-6380-z
更新日期:2019-12-03 00:00:00
abstract:BACKGROUND:Increasing evidence suggests that alterations in mitochondrial DNA (mtDNA) content may be implicated in the tumorigenesis of several malignancies. However, the association between mtDNA content in peripheral blood lymphocytes (PBLs) and glioma risk has not been investigated. METHODS:Real-time PCR was used t...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-14-680
更新日期:2014-09-19 00:00:00
abstract:BACKGROUND:Prospective trials have demonstrated the advantage of dose-escalated radiotherapy for the biochemical and clinical control of intermediate risk prostate cancer. Dose escalation improves outcomes but increases risks of urinary and bowel toxicity. Recently the contribution of "spacers" positioned in the septum...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-018-4492-5
更新日期:2018-05-16 00:00:00
abstract:BACKGROUND:A recent genome wide case-control association study identified NuMA region on 11q13 as a candidate locus for breast cancer susceptibility. Specifically, the variant Ala794Gly was suggested to be associated with increased risk of breast cancer. METHODS:In order to evaluate the NuMa gene for breast cancer sus...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-8-71
更新日期:2008-03-10 00:00:00
abstract:BACKGROUND:For gastric cancer patients, surgical resection with en-bloc lymphadenectomy is the cornerstone of curative treatment. Open gastrectomy has long been the preferred surgical approach worldwide. However, this procedure is associated with considerable morbidity. Several meta-analyses have shown an advantage in ...
journal_title:BMC cancer
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1186/s12885-015-1551-z
更新日期:2015-07-29 00:00:00
abstract:BACKGROUND:The purpose of the PROLUCA study is to investigate the efficacy of preoperative and early postoperative rehabilitation in a non-hospital setting in patients with operable lung cancer with special focus on exercise. METHODS:Using a 2 x 2 factorial design with continuous effect endpoint (Maximal Oxygen Uptake...
journal_title:BMC cancer
pub_type: 杂志文章,随机对照试验
doi:10.1186/1471-2407-14-404
更新日期:2014-06-04 00:00:00
abstract:BACKGROUND:Hypoxia is an important factor that contributes to tumour aggressiveness and correlates with poor prognosis and resistance to conventional therapy. Therefore, identifying hypoxic environments within tumours is extremely useful for understanding cancer biology and developing novel therapeutic strategies. Seve...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-019-5727-9
更新日期:2019-05-29 00:00:00
abstract:BACKGROUND:Metastatic breast cancer carries a poor prognosis despite the success of newly targeted therapies. Treatment options remain especially limited for the subtype of triple negative breast cancer (TNBC). Several signaling pathways, including NF-κB, are altered in TNBC, and the complexity of this disease implies ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-018-4507-2
更新日期:2018-05-25 00:00:00
abstract:BACKGROUND:Cigarette smoke is associated with the majority of lung cancers: however, 25% of lung cancer patients are non-smokers, and half of all newly diagnosed lung cancer patients are former smokers. Lung tumors exhibit distinct epidemiological, clinical, pathological, and molecular features depending on smoking sta...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-14-778
更新日期:2014-10-24 00:00:00
abstract:BACKGROUND:Colorectal cancer (CRC) is a heterogeneous disease with a complex biology and a wide number of altered genes such as BRAF, KRAS and PIK3CA. Advances with new-targeted therapies have been achieved and available treating options have prolonged patient's survival. However, BRAF-mutated CRC patients remain unres...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-020-6586-0
更新日期:2020-02-17 00:00:00
abstract:BACKGROUND:The epithelial cell adhesion molecule (EpCAM) has been shown to be overexpressed in breast cancer and stem cells and has emerged as an attractive target for immunotherapy of breast cancer patients. This study analyzes the effects of EpCAM on breast cancer cell lines with epithelial or mesenchymal phenotype. ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-12-501
更新日期:2012-10-30 00:00:00
abstract:BACKGROUND:Human Papillomavirus (HPV) genotyping has an increasingly important role in cervical cancer screening and vaccination monitoring, however, without an internationally agreed standard reference assay. The test results from the most widely used genotyping assays are read manually and hence prone to inter-observ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-015-1223-z
更新日期:2015-04-02 00:00:00
abstract:BACKGROUND:Patients with metastatic renal cell cancer (mRCC) are commonly treated with the tyrosine kinase inhibitor sunitinib, which blocks signalling from vascular endothelial growth factor (VEGF) - and platelet-derived growth factor-receptors, inhibiting development of new blood vessels. There are currently no predi...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-020-06770-z
更新日期:2020-04-22 00:00:00
abstract:BACKGROUND:Studies comparing the effect of antiangiogenic agents targeting different angiogenic pathways are sparse. The purpose of this study was to compare the effect of properdistatin and sunitinib treatment in a preclinical model of malignant melanoma. Properdistatin is a small peptide derived from the thrombospond...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-017-3404-4
更新日期:2017-06-12 00:00:00
abstract:BACKGROUND:We report an extremely rare case of vaginal clear cell carcinoma, which originated from the malignant transformation of vaginal adenosis without prenatal diethylstilbestrol (DES) exposure. CASE PRESENTATION:In this case, the patient was a Chinese woman with a history of two decades of intermittent vaginal p...
journal_title:BMC cancer
pub_type: 杂志文章,评审
doi:10.1186/s12885-019-6026-1
更新日期:2019-08-13 00:00:00
abstract:BACKGROUND:Distress in patients with cancer influences their quality of life. Worldwide, screening on distress with the Distress Thermometer (DT) in patients with cancer is recommended. However, the effects of the use of the DT on the psychosocial wellbeing of the patient are unknown. A study to assess the psychosocial...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-016-2565-x
更新日期:2016-07-25 00:00:00
abstract:BACKGROUND:Suppressor of cytokine signaling (SOCS) proteins comprise a protein family, which has initially been described as STAT induced inhibitors of the Jak/Stat pathway. Recent in vivo and in vitro studies suggest that SOCS proteins are also implicated in cancer. The STAT5 induced IGF-I acts as an endocrine and par...
journal_title:BMC cancer
pub_type: 杂志文章,多中心研究
doi:10.1186/1471-2407-7-136
更新日期:2007-07-25 00:00:00
abstract:BACKGROUND:Hemostatic alterations occur during the development of cancer. Plasma D-dimer is a hypercoagulability and fibrinolytic system marker that is increased in patients with various solid tumours. The aim of this study was to evaluate the hemostatic status of nasopharyngeal carcinoma (NPC) patients by assessing pl...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-14-583
更新日期:2014-08-10 00:00:00
abstract:BACKGROUND:In a phase I study of angiotensin-(1-7) [Ang-(1-7)], clinical benefit was associated with reduction in plasma placental growth factor (PlGF) concentrations. The current study examines Ang-(1-7) induced changes in biomarkers according to cancer type and investigates mechanisms of action engaged in vitro. MET...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-12-404
更新日期:2012-09-11 00:00:00
abstract:BACKGROUND:Tobacco smoke exposure may be associated with increased breast cancer risk, although the evidence supporting the association is inconclusive. We conducted a case-control study in Delaware, incorporating detailed exposure assessment for active and secondhand smoke at home and in the workplace. METHODS:Primar...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-8-157
更新日期:2008-06-02 00:00:00
abstract:BACKGROUND:The aim of this study was to determine the safety and efficacy of fractionated stereotactic radiotherapy (SRT) in combination with systemic therapies (ST) for brain metastases (BM). METHODS:Ninety-nine patients (171 BM) received SRT and concurrent ST (group 1) and 95 patients (131 BM) received SRT alone wit...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-020-07491-z
更新日期:2020-10-13 00:00:00
abstract:BACKGROUND:Afaitnib has shown anti-tumor activity against metastatic EGFR-mutated NSCLC after prior failure to first generation EGFR-TKI and chemotherapy. We prospectively evaluated the efficacy and safety of afatinib in Chinese patients who previously failed first-generation TKI and chemotherapy under a compassionate ...
journal_title:BMC cancer
pub_type: 临床试验,杂志文章
doi:10.1186/s12885-016-2201-9
更新日期:2016-02-24 00:00:00
abstract:BACKGROUND:Genomic alterations of the proto-oncogene c-erbB-2 (HER-2/neu) are associated with aggressive behavior and poor prognosis in patients with breast cancer. The variable clinical outcomes seen in patients with similar HER2 status, given similar treatments, suggests that the effects of amplification of HER2 can ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-8-297
更新日期:2008-10-14 00:00:00
abstract:BACKGROUND:The EGFR-T790M mutation is clinically detected using re-biopsy in approximately 50% of patients with acquired resistance to epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) in advanced non-small cell lung cancer (NSCLC) who harbor EGFR mutations. However, little is known about the popu...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-018-5153-4
更新日期:2018-12-11 00:00:00
abstract:BACKGROUND:Some historic breakthroughs have been made in immunotherapy of advanced cancer. However, there is still little research on immunotherapy in prostate cancer. We explored the relationship between immune cell infiltration and prostate cancer recurrence and tried to provide new ideas for the treatment of prostat...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-019-6391-9
更新日期:2019-12-03 00:00:00
abstract:BACKGROUND:Patients with BRAF mutation-positive advanced melanoma respond well to matched therapy with BRAF or MEK inhibitors, but often quickly develop resistance. METHODS:Tumor tissue from ten patients with advanced BRAF mutation-positive melanoma who achieved partial response (PR) or complete response (CR) on BRAF ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-015-1029-z
更新日期:2015-02-18 00:00:00
abstract:BACKGROUND:Tumor invasion through a basement membrane is one of the earliest steps in metastasis, and growth factors, such as Epidermal Growth Factor (EGF) and Hepatocyte Growth Factor (HGF), stimulate this process in a majority of solid tumors. Basement membrane breakdown is one of the hallmarks of invasion; therefore...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-017-3660-3
更新日期:2017-10-04 00:00:00