Abstract:
:Lung development progresses through a process reliant on mechanical cell stretch. However, this process is not well defined at the molecular level. Our goal was to globally analyze the transcriptome of fetal mouse lungs following short periods of tracheal occlusion (TO) to identify cellular processes that are rapidly modulated in response to intraluminal stretch increase. Serial analysis of gene expression (SAGE) was used to examine the global transcriptomic response of mouse prealveolar stage lungs to in vivo TO at 1 and 3 h. SAGE results were extended by histo- and immunochemical examination. Based on the 97 TO-modulated transcripts identified, our results further point out that continuous stretch in developing lungs leads directly to rapid and highly specific phenotypic modifications in a significant proportion of pulmonary cells. We conclude that intraluminal stretch increase during prealveolar stage of lung development induces a critical transition of pulmonary cells phenotype in which there is an increase in alpha-smooth muscle actin (alpha-SMA)-containing cells along with a relative decrease in lipid-containing cells.
journal_name
Pediatr Resjournal_title
Pediatric researchauthors
Seaborn T,St-Amand J,Cloutier M,Tremblay MG,Maltais F,Dinel S,Moulin V,Khan PA,Piedboeuf Bdoi
10.1203/PDR.0b013e31815eba47subject
Has Abstractpub_date
2008-02-01 00:00:00pages
124-30issue
2eissn
0031-3998issn
1530-0447journal_volume
63pub_type
杂志文章abstract::Defective arousal mechanisms are viewed as contributory to sleep hypopnea disorders and sudden infant death syndrome. Sighs (i.e. augmented breaths) as well as startles have not traditionally been viewed as arousal-related phenomena in infants. We hypothesized that, if sighs and startles are the initial event in a seq...
journal_title:Pediatric research
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journal_title:Pediatric research
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journal_title:Pediatric research
pub_type: 杂志文章,评审
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journal_title:Pediatric research
pub_type: 杂志文章
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journal_title:Pediatric research
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journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199802000-00003
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journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-198208000-00008
更新日期:1982-08-01 00:00:00
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journal_title:Pediatric research
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doi:10.1203/00006450-198002000-00019
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doi:10.1203/00006450-200010000-00012
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journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199804000-00006
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journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199211000-00016
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journal_title:Pediatric research
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journal_title:Pediatric research
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doi:10.1203/00006450-198106000-00005
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journal_title:Pediatric research
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更新日期:2006-05-01 00:00:00
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journal_title:Pediatric research
pub_type: 杂志文章
doi:10.1203/00006450-199709000-00010
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