Abstract：
:K(+)-p-nitrophenylphosphatase (K(+)pNPPase) is the enzyme, which is considered to be involved in K(+)-dependent hydrolysis of the phosphoenzyme in the reaction cycle of Na(+), K(+)ATPase. The aim of our present study was to characterize some features of K(+)pNPPase in homogenates of the rat brain and liver. We determined p-nitrophenylphosphatase (pNPPase) activity in the presence of various ion combinations (Mg(2+)+ K(+), Mg(2+), K(+)). We found a higher total pNPPase activity in the brain (0.8+/-0.079 nkat/mg protein) than in the liver (0.08+/-0.01 nkat/mg protein). Contrary to the liver, the main part of the total brain activity was K(+)-dependent. The activity of K(+)pNPPase was significantly higher in cerebral cortex homogenates (0.86+/-0.073 nkat/mg protein) in comparison to those of the whole brain (0.57+/-0.075 nkat/mg protein). The specific K(+)pNPPase activity was two times higher in the isolated pellet fraction (0.911+/-0.07 nkat/mg protein), rich in synaptosomes, compared to the whole brain homogenate (0.57+/-0.075 nkat/mg protein). Our results demonstrate the high activity of K(+)pNPPase in the brain tissue and its distribution mainly into the pellet fraction, what might indicate a possible role of K(+)pNPPase in specific structures of the brain, e.g. in synaptosomes.
journal_name
Physiol Resjournal_title
Physiological researchauthors
Durfinová M,Brechtlová M,Líska B,Barosková Zsubject
Has Abstractpub_date
2009-01-01 00:00:00pages
121-6issue
1eissn
0862-8408issn
1802-9973pii
1191journal_volume
58pub_type
杂志文章abstract：:Oculometric behaviour assessed by pupil response provides important information about central autonomic activity and emotional regulation. However, studies regarding pupil response to emotional stimuli in adolescent depression are rare. We aimed to study emotional-linked pupil response in adolescent depression. Twenty...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.934359
更新日期：2019-12-20 00:00:00
abstract：:The main role of research in medicine is to provide relevant knowledge which, after successful translation to clinical practice, improves the quality of healthcare. The sex bias which is still present in the majority of research disciplines prefers male subjects despite legislation changes in the US grant agencies and...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.934593
更新日期：2020-12-31 00:00:00
abstract：:Effects of early neonatal interventions on metabolic parameters later in life (s.c. late effects) were studied in rats using two models; namely, (a) the effects of premature weaning and (b) the effects of "dietary" manipulations during the suckling period (s.c. small vs. large litters). (a) Premature weaning of rats c...
journal_title：Physiological research
pub_type： 杂志文章,评审
doi：
更新日期：1995-01-01 00:00:00
abstract：:We present the current state of complex circulatory dynamics model development based on Guyton's famous diagram. The aim is to provide an open-source model that will allow the simulation of a number of pathological conditions on a virtual patient including cardiac, respiratory, and kidney failure. The model will also ...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2010-01-01 00:00:00
abstract：:To estimate the significance of insulin in the regulation of preimplantation embryo growth, female mice received a single subdiabetogenic dose of streptozocin (65 mg/kg intraperitoneally) 8-11 days or 14-17 days before fertilization. Mean glycaemia levels and the number of embryos per mouse did not differ significantl...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:We measured plasma concentrations, adipose tissue and placental mRNA expression of hepatokines fetuin A, fetuin B and fibroblast growth factor 21 (FGF21) in 12 healthy pregnant women (P group), 12 pregnant women with gestational diabetes (GDM) and 10 healthy non-pregnant women (N group) to explore their potential role...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.934017
更新日期：2018-11-28 00:00:00
abstract：:It has been reported that the major function of the sterol regulatory element binding protein 2 (SREBP-2) is to activate preferentially cholesterol biosynthesis in liver and adipose tissue rather than fatty acid synthesis. In the current study, we analyzed the effects of overexpression of human dominant-positive SREBP...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.932751
更新日期：2014-01-01 00:00:00
abstract：:We studied the circadian oscillation of lipid peroxides (TBARS) in the pineal gland of rats adapted to light:dark 12:12 h regimen. The concentration of TBARS was determined at 3-h intervals during 24 hours. TBARS of pineal gland oscillated rhythmically during the 24 h period. The maximal concentration of lipoperoxidat...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：:It was recognized that recombinant inbred strains are a very powerful system for the study of the genetics of hypertension, linkage analysis and gene mapping. Such set of recombinant inbred strains has been developed in the cooperation of Prof V. Kren and Dr. M. Pravenec in Prague. These recombinant inbred strains wer...
journal_title：Physiological research
pub_type： 社论
doi：
更新日期：1993-01-01 00:00:00
abstract：:The goal of this study is to evaluate if promotion of angiogenesis by systemic treatment with an antagomir against miR-92a, a well established inhibitor of angiogenesis, will maximize the benefits of exercise on bone. Ten week old female C57BL6/J mice were subjected to two weeks of external load by four point bending....
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.932410
更新日期：2013-01-01 00:00:00
abstract：:The aim of the study was to evaluate short-term heart rate variability (HRV) as an index of cardiac autonomic control in rats with lipopolysaccharide (LPS)-induced endotoxemia. Animals were injected intraperitoneally with LPS (100 microg/kg b.w.) and control group with an equivalent volume of saline. ECG recordings we...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.933226
更新日期：2015-01-01 00:00:00
abstract：:Fibroblast growth factor-21 (FGF-21) has been recently characterized as a new adipokine. The aim of this study was to assess FGF-21 levels in patients with rheumatoid arthritis (RA) and osteoarthritis (OA) and to study the relationship between FGF-21, disease activity and metabolic status. The levels of FGF-21 in seru...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.932324
更新日期：2012-01-01 00:00:00
abstract：:To predict more precisely the effect of stobadine, a pyridoindole antioxidant agent, in the whole organism, we studied its effect on opsonized zymosan-stimulated free radical generation in whole blood, on superoxide generation in the mixture of PMNL : platelets (1:50), as well as on superoxide generation and myelopero...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:The expression of aquaporins (AQPs) in the fetal porcine urinary tract and its relation to gestational age has not been established. Tissue samples from the renal pelvis, ureter, bladder and urethra were obtained from porcine fetuses. Samples were examined by RT-PCR (AQPs 1-11), QPCR (AQPs positive on RT-PCR), and imm...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.933545
更新日期：2018-05-04 00:00:00
abstract：:Anthracyclines, e.g. doxorubicin, pirarubicin, are widely used as cytostatic agents in the polymer nanotherapeutics designed for the highly effective antitumor therapy with reduced side effects. However, their precise dosage scheme needs to be optimized, which requires an accurate method for their quantification on th...
journal_title：Physiological research
pub_type： 杂志文章,评审
doi：10.33549/physiolres.933140
更新日期：2015-01-01 00:00:00
abstract：:Spontaneous activity of cortical neurons exhibits alternative fluctuations of membrane potential consisting of phased depolarization called "up-state" and persistent hyperpolarization called "down-state" during slow wave sleep and anesthesia. Here, we examined the effects of sound stimuli (noise bursts) on neuronal ac...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.932086
更新日期：2011-01-01 00:00:00
abstract：:Dihydrotestosterone (DHT) originates via irreversible reduction of testosterone by catalytic activity of 5alpha-reductase enzyme and it is demonstratively the most effective androgen. Androgens influence adipose tissue in men either directly by stimulation of the androgen receptor or indirectly, after aromatization, b...
journal_title：Physiological research
pub_type： 杂志文章,评审
doi：10.33549/physiolres.932080
更新日期：2011-01-01 00:00:00
abstract：:We sought to examine the association between maternal erythrocyte omega-3, omega-6 and trans fatty acids and risk of preeclampsia. We conducted a case-control study of 170 women with proteinuric, pregnancy-induced hypertension and 185 normotensive pregnant women who delivered at Harare Maternity Hospital, Harare, Zimb...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：:Pulse dye densitometry (PDD) enables the evaluation of hemodynamic state as well as liver function. A repeated examination, even after a short pause (or under stress condition), enables to follow safely the dynamics of liver pathology. From presented parameters we have evaluated as reliable the C5-clearance, an expres...
journal_title：Physiological research
pub_type： 临床试验,杂志文章
doi：
更新日期：2009-01-01 00:00:00
abstract：:Senile dementia of Alzheimer's type (AD) is commonly characterized as a neurodegenerative disorder, which exhibits gradual changes of consciousness, loss of memory, perception and orientation as well as loss of personality and intellect. AD prevalence increases dramatically with age and is the fourth cause of death in...
journal_title：Physiological research
pub_type： 杂志文章,评审
doi：
更新日期：2001-01-01 00:00:00
abstract：:The aim of this study was to investigate the effects of rooibos tea as a natural source of a wide scale of antioxidants on the prevention and treatment of oxidative stress in streptozotocin-induced diabetic rats. Expected significant changes of biochemical parameters characteristic for experimental diabetic state were...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：:The objective of the present experiment was to assess the involvement of small intestine in expression of susceptibility or resistance to the high-fat/high-energy diet. The investigation was carried out in adult male Sprague-Dawley rats fed either standard laboratory diet (3.2 kcal/g, 9.5 % fat) or high-fat (HF) diet ...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:The influence of cilostazol on learning and memory, and cyclin D1 expression in the cerebral cortex of rats with chronic cerebral ischemia were investigated. A chronic cerebral ischemia model was established using the permanent bilateral common carotid artery occlusion method (2VO), learning and memory capacity was de...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.934282
更新日期：2020-08-31 00:00:00
abstract：:Hemodynamics in the distal end-to-side anastomosis is related to early development of intimal hyperplasia and bypass failure. In this study we investigated the effect of diameter ratios between the target artery and the bypass at three different angles of the connection. The pulsatile flow field was visualized using p...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.933297
更新日期：2016-12-13 00:00:00
abstract：:Remote ischemic preconditioning (RIPC) is a novel strategy of protection against ischemia-reperfusion (IR) injury in the heart (and/or other organs) by brief episodes of non-lethal IR in a distant organ/tissue. Importantly, RIPC can be induced noninvasively by limitation of blood flow in the extremity implying the app...
journal_title：Physiological research
pub_type： 杂志文章,评审
doi：10.33549/physiolres.933392
更新日期：2016-09-19 00:00:00
abstract：:Steroids are important markers in pregnancy. Although estimating their levels separately in umbilical arterial (UA) and venous blood (UV) enable more precise insights into the functioning fetoplacental unit compared to using mixed umbilical blood (UM), selective aspiration of UA and UV is technically more demanding th...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.932624
更新日期：2014-01-01 00:00:00
abstract：:The effects of selenium (Se) on antioxidant defense system in liver and kidneys of rats with cadmium (Cd)-induced toxicity were examined. Cd exposure (15 mg Cd/kg b.m./day as CdCl(2) for 4 weeks) resulted in increased lipid peroxidation (LP) in both organs (p<0.005 and p<0.01). Vitamin C (Vit C) was decreased in the l...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:Precise and efficient endocytosis is critical for sustained neurotransmission during continuous neuronal activity. Endocytosis is a prerequisite for maintaining the auditory function. However, the differences between the patterns of endocytosis in cochlear inner hair cells (IHCs) and outer hair cells (OHCs) remain unc...
journal_title：Physiological research
pub_type： 杂志文章
doi：10.33549/physiolres.934009
更新日期：2019-08-29 00:00:00
abstract：:Exposure to hyperbaric oxygen leads to increased amount of reactive oxygen species (ROS) that are derived from various sources. After the discovery that ROS can function as signaling molecules, the idea of ROS being hazardous to biological tissues has been challenged. The aim of this study was to examine the changes i...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:Neonatal exposure to hyperoxia alters lung development in mice. We tested if retinoic acid (RA) treatment is capable to affect lung development after hyperoxic injury and to maintain structural integrity of lung. The gene of vascular endothelial growth factor A (VEGF-A) is one of the RA-responsive genes. Newborn BALB/...
journal_title：Physiological research
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
