Abstract:
:Nuclear binding of cytoplasmic estrogen receptors was measured in an in vitro cell-free system, using various mixtures of cytosols and nuclei from uteri of mature (6-9 month old) and senescent (24-25 month old) Wistar rats. Both nuclei and cytoplasmic receptors from senescent uteri were 25-35% less efficient in supporting nuclear binding than those obtained from mature tissues as evidenced by the concentrations of occupiable nuclear acceptor sites. No age differences in association or dissociation constants were observed for the nuclear binding reaction. However, the apparent inability of some aged receptors to bind to the full complement of mature nuclear acceptor sites may indicate a qualitative deficiency in the cytosols of senescent uteri.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Chuknyiska RS,Haji M,Foote RH,Roth GSdoi
10.1210/endo-116-2-547subject
Has Abstractpub_date
1985-02-01 00:00:00pages
547-51issue
2eissn
0013-7227issn
1945-7170journal_volume
116pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::The adipose conversion of 3T3-L1 fibroblasts depends on serum factors, one of which has been identified as GH. Human serum extracts obtained by heat treatment do not contain intact GH, as shown by RIA, but still support adipose conversion. After organic solvent extraction followed by reversed phase HPLC, two adipogeni...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-2-469
更新日期:1987-02-01 00:00:00
abstract::The existence of a vasoactive molecule released in response to hCG is believed to be the main feature in the development of ovarian hyperstimulation syndrome (OHSS) in women, and vascular endothelial growth factor (VEGF) is the main candidate as the human chorionic gonadotropin (hCG) mediator. This study was conducted...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220204
更新日期:2002-11-01 00:00:00
abstract::This study was aimed at testing the hypothesis that different forms of fibronectin (FN), produced as a consequence of the alternative splicing of the precursor messenger RNA, play specific roles during development of the ovarian follicle. In particular, we were interested in determining the effect of the ED-I (also te...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.6.6708
更新日期:1999-06-01 00:00:00
abstract::Women with type 1 diabetes are subfertile. Diabetes negatively affects pregnancy by causing early miscarriage and poor prenatal outcomes. In this study we examine consequences of maternal type 1 diabetes on early embryo development, metabolic gene expression, and the pattern of insulin receptor (IR) and IGF-I receptor...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0187
更新日期:2010-09-01 00:00:00
abstract::While uterine stromal cells (USC) appear to modify the function of uterine epithelial cells (UEC) under certain conditions in vivo, relatively little is known about the effect of epithelial cells on stromal cell differentiation and function. To determine if UEC modulate USC function in vitro, highly enriched (> 95%) c...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.6.1446600
更新日期:1992-12-01 00:00:00
abstract::The 3'5'-cyclic GMP (cGMP) pathway is known to influence ovarian functions, including steroidogenesis, ovulation, and granulosa cell proliferation. We show here that cGMP-phosphodiesterase (PDE) activity increased in a gonadotropin-dependent manner more than 3-fold in the cumulus-oocyte complex (COC) after 24 h in vit...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0547
更新日期:2008-11-01 00:00:00
abstract::To elucidate the mechanism of humoral hypercalcemia elicited by human esophageal carcinoma cells (EC-GI), which constitutively produced interleukin-1 alpha (IL-1 alpha) and PTH-like factor, the effects of IL-1 alpha and PTH-related protein (PTH-rP) on bone resorption in vitro and on serum calcium concentrations in viv...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-5-2172
更新日期:1989-05-01 00:00:00
abstract::Recently, we have reported that retinoic acid (RA), similarly to retinol acetate, is able to reinitiate spermatogenesis in vitamin A-deficient rats. Here, we investigated the expression of RA receptors RAR alpha, RAR beta, RAR gamma, and retinoid X receptor RXR alpha by Northern blot analysis of poly(A)+ RNA of testes...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.1.1319320
更新日期:1992-07-01 00:00:00
abstract::The primary culture of rat luteal cells and their long-term maintenance have been difficult. Low cellular yields have limited the possibility for the study of gene regulation in luteal cells. The goal of this study was to develop a cell line to serve as a model by which to study the expression and regulation of variou...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.4.5929
更新日期:1998-04-01 00:00:00
abstract::Bone morphogenetic proteins (BMPs) comprise one of the largest subgroups in the TGF-β ligand superfamily. We have identified a functional BMP system equipped with the ligand (BMP4), receptors (BMP type II receptor, BMP type IA receptor, also called ALK3) and the signaling proteins, namely the mothers against decapenta...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2014-1942
更新日期:2015-07-01 00:00:00
abstract::The role of calcium in the angiotensin II- or potassium-mediated increase in aldosterone production was analyzed in isolated glomerulosa cells prepared from bovine adrenal glands. The response to potassium was highly dependent on the extracellular calcium concentration, and a maximal response was observed at 0.5 mM ca...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-109-6-2196
更新日期:1981-12-01 00:00:00
abstract::Exposure of the ileum to nutrients markedly inhibits several upper gastrointestinal functions. Hormonal peptides of the ileal wall, i.e. peptide YY (PYY), glucagon-like peptide-1 (GLP-1), and neurotensin (NT), are thought to play a role in this negative feedback mechanism. The present study was conducted to comparativ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.9.6202
更新日期:1998-09-01 00:00:00
abstract::GnRH peptides in the hypothalami of marsupials (tammar wallaby, short-nosed bandicoot, and eastern quoll) and a monotreme (echidna) were investigated by reverse phase HPLC and RIA with region-specific antisera. In the wallaby hypothalamic extract, a single form of GnRH was present, which eluted in the same position as...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-5-2244
更新日期:1989-11-01 00:00:00
abstract::Calcitonin gene-related peptide (CGRP)-I has been reported to inhibit gastric acid secretion through stimulation of gastric somatostatin-14 (S-14) release. To establish whether some of the effects of CGRP-I on intestinal function might also be mediated through somatostatin, fetal rat intestinal cultures were treated w...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.6.7902270
更新日期:1993-12-01 00:00:00
abstract::Hereditary vitamin D-resistant rickets (HVDRR) is an autosomal recessive disease caused by mutations in the vitamin D receptor (VDR). We studied a young Saudi Arabian girl who exhibited the typical clinical features of HVDRR, but without alopecia. Analysis of her VDR gene revealed a homozygous T to C mutation in exon ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0080
更新日期:2004-11-01 00:00:00
abstract::The present study examined the effects of FSH and insulin (IN) on 17 beta-estradiol (E2) secretion and granulosa cell proliferation. For these studies, immature rat ovaries were maintained in perifusion culture and continuously exposed to FSH (0-800 ng RP-1 eq/ml) and /or IN (0.2 U/ml). At specific times, the ovaries ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-1-191
更新日期:1991-01-01 00:00:00
abstract::The effects of K+ depletion and ouabain (0.1, 0.5, and 1 mM) on glucagon and insulin release from the in vitro perfused rat pancreas were studied in the absence or presence of arginine (10 mM). In the absence of arginine, K+ depletion resulted in an inhibition of glucagon release, both at a low (2.8 mM) and a higher (...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-113-1-178
更新日期:1983-07-01 00:00:00
abstract::We investigated the effect of central serotonin (5-hydroxytryptamine, 5-HT) administration on hypothalamic tuberoinfundibular dopamine neurons and related changes in neuronal activity to circulating PRL levels in two physiological models: 1) pregnant rats expressing (day 8) or not expressing (days 11 and 16) PRL surge...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.6.1359960
更新日期:1992-12-01 00:00:00
abstract::Transfer of corticosterone from serum to milk was studied in lactating rats after ether and pentobarbital anesthesia. Two minutes after the onset of anesthesia stress, total serum and milk corticosterone concentrations were not significantly different. By 30 min, corticosterone concentrations in serum increased to a p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-4-1364
更新日期:1984-10-01 00:00:00
abstract::GRK2 is a member of the G protein-coupled receptor kinase family that phosphorylates the activated form of beta-adrenergic and other G protein-coupled receptors and plays an important role in their desensitization and modulation. Alterations in thyroid hormone levels have been reported to lead to important changes in ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.3.8039
更新日期:2001-03-01 00:00:00
abstract::p-Tyramine, an endogenous amine with sympathomimetic action, is found in the mammalian hypothalamus. When injected ip, p-tyramine reduced serum PRL without altering LH and TSH serum titers in adult orchidectomized rats and rats subjected to ether or immobilization stress. The hyperprolactinemia achieved by this last p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-5-2044
更新日期:1985-05-01 00:00:00
abstract::Canine renal cortical PTH receptors were solubilized after occupancy of membrane-associated receptors with the agonist ligand [125I]bovine (b) PTH-(1-34). Stabilization of binding during solubilization required the use of high concentrations of BSA (optimally 5%) and appropriate detergents (0.5% 3-[(3-cholamidopropyl)...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-118-3-932
更新日期:1986-03-01 00:00:00
abstract::The receptor for the orexigenic peptide, ghrelin, is one of the most constitutively active 7TM receptors known, as demonstrated under in vitro conditions. Change in expression of a constitutively active receptor is associated with change in signaling independent of the endogenous ligand. In the following study, we fou...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-1638
更新日期:2009-11-01 00:00:00
abstract::Hypothalamic proopiomelanocortin (POMC)-derived MSH peptides and the melanocortin receptor antagonist, agouti-related protein (AgRP), interact to regulate energy balance. Both POMC and AgRP neurons express estrogen receptors, but little is known about estrogen regulation of the melanocortin system in the primate. We h...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0853
更新日期:2010-03-01 00:00:00
abstract::The ability of the adult pancreas to generate new insulin (beta) cells has been controversial because of difficulties in unequivocally identifying the precursor population. We recently determined that beta cells were generated during development from precursors that expressed the homeodomain-containing transcription f...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.4.5049
更新日期:1997-04-01 00:00:00
abstract::Osteocalcin (OC), a bone specific protein expressed during differentiation and mineralization of the bone extracellular matrix, is down-regulated upon treatment with transforming growth factor (TGF)-beta 1. To address the potential role of OC gene expression in relation to TGF-beta 1 regulation of bone formation and r...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.137.5.8612540
更新日期:1996-05-01 00:00:00
abstract::Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine that plays a pivotal role in several immunoinflammatory and autoimmune diseases. In this study we examined the role of MIF in the development of immunoinflammatory diabetes induced in susceptible strains of mice by multiple low doses of strepto...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-1393
更新日期:2005-07-01 00:00:00
abstract::We used the library subtraction technique to identify genes specifically expressed in the rat uterus during early pregnancy. One such gene was that for stathmin, a factor that is associated with tubulin binding and the destabilization of microtubules. Stathmin was expressed at higher levels in implantation sites than ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220834
更新日期:2003-04-01 00:00:00
abstract::The pharmacokinetics of TRH and its metabolite deamido-TRH (TRH-OH) were evaluated and compared. After a 60- to 90-min infusion of the appropriate peptide into rats, the MCR, half-life of disappearance (t 1/2), and volume of distribution were calculated. The MCR of TRH averaged 4 ml/min, whereas that of TRH-OH was 6.5...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-3-1031
更新日期:1984-09-01 00:00:00
abstract::We investigated the effects of acute (24-h) peroxisome proliferator-activated receptor (PPAR)alpha activation by WY14,643 (pirinixic acid) treatment on glucose-stimulated insulin secretion (GSIS) during pregnancy, in the rat, in relation to insulin sensitivity. GSIS after iv glucose challenge (500 mg/kg) was increased...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220811
更新日期:2003-01-01 00:00:00