Abstract:
:Treatment of purified simian virus 40 large T antigen (LT) with protein phosphatase 2A stimulates LT-dependent DNA unwinding and replication (D. M. Virshup, M. G. Kauffman, and T. J. Kelly, EMBO J. 8: 3891-3898, 1989). The specificity of the catalytic subunit of protein phosphatase 2A toward LT was investigated by two-dimensional peptide mapping. Increasing amounts of phosphatase sequentially removed the phosphates from serine residues 120, 123, 677, and perhaps 679, residues which have been implicated in regulating the DNA-binding activity of LT.
journal_name
J Viroljournal_title
Journal of virologyauthors
Scheidtmann KH,Virshup DM,Kelly TJdoi
10.1128/JVI.65.4.2098-2101.1991subject
Has Abstractpub_date
1991-04-01 00:00:00pages
2098-101issue
4eissn
0022-538Xissn
1098-5514journal_volume
65pub_type
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