[mRNA expression analysis and classification of colonic biopsy samples using oligonucleotide and cDNA microarray techniques].

Abstract:

:Despite tremendous progress in the past few decades, certain important aspects regarding the diagnosis, therapy, and follow-up of colorectal cancer still remain unsolved. In our work we searched for biomarkers of the development of colorectal carcinoma, and performed gene expression analysis for colorectal disease classification. We have established that the oligonucleotide microarray analyses of biopsy samples wholly fulfil the Affymetrix quality requirements, are highly standard and reproducible and the Taqman microfluidic card system is suitable for high-throughput, quick and cost efficient real-time-PCR validation of gene expression changes. We have shown that the sequential overexpression of osteopontin and osteonectin mRNAs and proteins significantly correlates with the progression of the colorectal adenoma-dysplasia-carcinoma sequence. We have identified and validated ten novel markers with continuously increasing mRNA expression in line with the adenoma-dysplasia-carcinoma transition. We have identified the top 27, 13 and 10 genes associated with adenoma, colorectal cancer, and inflammatory bowel diseases.

journal_name

Orv Hetil

journal_title

Orvosi hetilap

authors

Galamb O

doi

10.1556/OH.2008.28397

subject

Has Abstract

pub_date

2008-07-20 00:00:00

pages

1373-7

issue

29

eissn

0030-6002

issn

1788-6120

pii

RX0804736H772022

journal_volume

149

pub_type

杂志文章
  • [The role of transesophageal echocardiography in the detection of coronary anomalies and anatomical variations].

    abstract:OBJECTIVES:The aim of the study is to examine the role of transesophageal echocardiography (TEE) in the diagnosis of anomalies and anatomic variations of the coronary arteries. BACKGROUND:In the past, coronary angiography was the only method for diagnostic confirmation in all cases with coronary anomalies, but even du...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章

    doi:

    authors: Apró D,Lupkovics G,Mezey B

    更新日期:1998-09-13 00:00:00

  • [Management of penile cancer, 2009].

    abstract:UNLABELLED:Penile cancer is a rare anomaly. Primary tumor and lymphnodes metastasis managements are essential. Nowadays, there is a growing wish for less invasive but curative treatment. AIMS AND METHODS:Newest opportunities of the management of penile cancer are reviewed according to the recent literature by the auth...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章,评审

    doi:10.1556/OH.2010.28787

    authors: Nyirády P,Riesz P

    更新日期:2010-04-11 00:00:00

  • [First detection of human astrovirus in gastroenteritis outbreak in Hungary].

    abstract:UNLABELLED:Human astroviruses are one of the known pathogens of gastroenteritis in infants, children and rarely in elderly. It causes 4.2-7.3% of the sporadic gastroenteritis cases with diarrhea and vomiting in children. The etiological role of astrovirus has not been confirmed yet in outbreaks of gastroenteritis in Hu...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章

    doi:10.1556/OH.2011.29013

    authors: Pankovics P,Boros Á,Rovács M,Nagy E,Krisztián E,Vollain M,Reuter G

    更新日期:2011-01-09 00:00:00

  • [Dissolving anxiety as an essential ingredient for any therapy].

    abstract::Anxiety frequently develops in human life and it is usually useful for the organism. Anxiety helps accomodation to the social environment, however, it may be harmful when it is intensive and long-lasting. It can also cause psychosomatic diseases. Anxiety may present itself as mild or moderate stress, psychosomatic dis...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章,评审

    doi:10.1556/OH.2014.29925

    authors: Radics J

    更新日期:2014-06-01 00:00:00

  • [Esophageal dilatation using the Savary-Gilliard sound set].

    abstract::Authors present a method of esophageal dilatation and report their experiences. The place of esophageal dilatation among other therapeutic approaches of esophageal stricture is discussed. The advantage of Savary-Gilliard bougies and guide wire and its applications are presented. During one and a half year 218 esophage...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章,评审

    doi:

    authors: Szendrényi V,Lázár G,Oláh T,Horváth Ors P

    更新日期:1992-11-15 00:00:00

  • [Effect of paracetamol on the acid hemolysis of erythrocytes, with special reference to the intracellular glutathione level].

    abstract::The authors give account of their study concerning the effect of paracetamol on the acid haemolysis, and glutathione content of human erythrocytes. In vitro, paracetamol decreased intracellular glutathione content in a dose dependent manner. This may lead to an increased sensitivity of erythrocytes to the haemolytic e...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章

    doi:

    authors: Altorjay I,Sári B,Imre S,Balla G,Dalmi L

    更新日期:1993-10-10 00:00:00

  • [Role of ultrasonography in the urgent differential diagnosis of acute scrotum].

    abstract:UNLABELLED:Acute scrotum, when testicular torsion is suspected, needs emergency exploration. However, acute scrotum caused by torsion of the Morgagni hydatid and epididymitis can be managed conservatively. Real time colour Doppler ultrasound is becoming a more and more popular tool in the differential diagnosis, howeve...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章

    doi:10.1556/OH.2011.29129

    authors: Sándor L,Gajda T,Aranyi V,Csízy I,Cserni T

    更新日期:2011-06-05 00:00:00

  • [The role of HDL in the prevention of cardiovascular events].

    abstract::The Framingham and other epidemiological studies have shown HDL and triglyceride to be inversely correlated. An independent relationship between HDL cholesterol and CAD was observed in other studies and also suggested by interventional trials. Decreased HDL-C and mildly elevated LDL-C are common in patients with coron...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章,评审

    doi:

    authors: Paragh G,Harangi M

    更新日期:2001-01-21 00:00:00

  • [Epidemiology of early infections after autologous hematopoietic stem cell transplantation. Analysis of data from 699 patients treated in a Hungarian centre].

    abstract::Introduction: Autologous hemopoietic stem cell transplantation remains a promising therapy in certain malignant and non-malignant conditions. The procedure, however, will increase the risk of complications, most notably early and late infections. Aim: To analyze the frequency and spectrum of pathogens in early (<+100 ...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章

    doi:10.1556/650.2020.31638

    authors: Lakatos B,Szabó H,Csordás K,Tatai G,Nikolova R,Csomor J,Reményi P,Masszi T,Vályi-Nagy I,Sinkó J

    更新日期:2020-01-01 00:00:00

  • [Clinical proarrhythmias induced by anti-arrhythmic drugs, non-cardiovascular agents and implantable cardioverter-defibrillators].

    abstract::The authors survey the electrophysiological mechanisms and clinical spectrum of the aggravation of cardiac arrhythmias provoked by antiarrhythmic drugs, non-cardiovascular agents and implantable cardioverter-defibrillators. The main aspects of the recognition, the differential diagnostics and the therapy are discussed...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章,评审

    doi:

    authors: Fazekas T,Liszkai G

    更新日期:2002-01-13 00:00:00

  • [Successful twin delivery following reduction of quadruplet pregnancy].

    abstract::The authors report first time in the Hungarian literature on multifetal pregnancy reduction: a quadruplet pregnancy was reduced to twins on transabdominal way in the 16th week of gestation on request of the parents. The quadruplets resulted from a forcefully induced ovulation. First weeks of gestation were complicated...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章,评审

    doi:

    authors: Patkós P,Csécsei K,Tóth-Pál E,Szabó I,Hruby E,Papp Z

    更新日期:1996-11-03 00:00:00

  • [Study of the canalis mandibulae morphology in different vertical facial types].

    abstract:INTRODUCTION:The precise knowledge of the position of canalis mandibulae and foramen mandibulae is important for surgical and endodontic interventions on the mandible. AIM:The aim of the study is to examine the position of these anatomical landmarks in different vertical facial types. METHOD:In the panorama radiograp...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章

    doi:10.1556/650.2017.30864

    authors: Mártha I K,Bica C,Kész B,Kerekes Máthé B

    更新日期:2017-11-01 00:00:00

  • [The level of use of statistical data in articles in the Orvosi Hetilap].

    abstract::The objective was to assess the level of use of statistics in the articles of the Orvosi Hetilap (OH). Eight clinical and 25 randomly selected general medical papers (published in 1992) were evaluated with the help of checklists used in the British Medical Journal (BMJ). The result was compared with the survey conduct...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章,评审

    doi:

    authors: Vitrai J,Czobor P

    更新日期:1996-03-03 00:00:00

  • [Prediction of the disease course in inflammatory bowel diseases].

    abstract::Clinical presentation at diagnosis and disease course of both Crohn's disease (CD) and ulcerative colitis (UC) are heterogeneous and variable over time. During follow up, intestinal strictures or perforation may eventually develop at most patients with CD, and significant number of patients will undergo surgery. Much ...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章,评审

    doi:10.1556/OH.2010.28813

    authors: Kiss LS,Lakatos PL

    更新日期:2010-02-21 00:00:00

  • [Novel treatment modalities in cutaneous T cell lymphoma. Biologic response modifiers].

    abstract::Biologic response modifiers. Biologic response modifiers such as IFN-alpha, retinoids and extracorporeal photopheresis have been widely used in the treatment of cutaneous lymphoma. The novel treatment modalities like cytokine therapies, monoclonal antibodies and fusion proteins have been used only in limited number of...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章,评审

    doi:

    authors: Marschalkó M

    更新日期:2005-06-05 00:00:00

  • [The effect of smoking on the quality of life enhancement following prosthetic rehabilitation in head and neck cancer patients who underwent surgical treatment].

    abstract:INTRODUCTION:It is known that the quality of life (QoL) of patients surgically treated for head and neck cancer (HNC) is significantly enhanced by rehabilitation. It is also known that some of these patients will not quit smoking. AIM:To assess if smoking hampers rehabilitation-related QoL enhancement after surgery. ...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章

    doi:10.1556/650.2017.30639

    authors: Nagy J,Novák P,Buzás K,Nagy K,Antal M

    更新日期:2017-02-01 00:00:00

  • [Postural tachycardia syndrome].

    abstract::Erect posture could be a source of diverse chronic symptoms usually referred as orthostasis intolerance. Increasing number of patients suffering from orthostatic syndromes is recognized. Characterization and distinction of these orthostatic syndromes are based on the clinical presentation; including the typical histor...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章,评审

    doi:

    authors: Makai A,Tahin T,Simor T,Csanádi Z,Rudas L

    更新日期:2005-03-13 00:00:00

  • [Undifferentiated connective tissue disease].

    abstract::Evolution of immunopathological diseases is usually slow and progressive. Non-differentiated collagen disease (NDC) or the term "undifferentiated connective tissue disease" (UCTD) represents a stage of disease where clinical symptoms and serological abnormalities suggest autoimmune disease, but they are not sufficient...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章,评审

    doi:10.1556/OH.2009.28610

    authors: Bodolay E,Szegedi G

    更新日期:2009-05-10 00:00:00

  • [Papillary renal cell carcinoma].

    abstract::The authors present a case of a papillary type renal cell carcinoma. The tumor is mostly discovered accidentally; a histopathological evaluation is indispensable for an exact diagnosis. Because of a better prognosis, differentiation of this tumor type from the classic variant of renal cell carcinoma is necessary, howe...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章

    doi:

    authors: Glasz T,Rusz A,Doros A,Schaff Z

    更新日期:2001-08-12 00:00:00

  • [Antioxidant effect of vitamin E in experimental hyperlipidemia].

    abstract::The effect of Vitamin E treatment was studied in experimental hyperlipidaemia. The male Wistar rats got control diet and parallel fat rich diet contained 2% cholesterol, 0.5% cholic acid and 20% sunflower oil added into LATI food during 9 days. The treated hyperlipidemic animals got vitamin E for 9 days in daily 8.56 ...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章

    doi:

    authors: Horváth EM,Blázovics A,Kemény T,Vásárhelyi B,Weinbrenner Z,Fehér J

    更新日期:1993-08-08 00:00:00

  • [Assessment of patient-reported outcomes by questionnaires in patients with Moderate and severe chronic obstructive pulmonary disease treated with glycopyrroniUm in the reaL lifE setTing in Hungary (AMULET)].

    abstract::Introduction: Chronic obstructive pulmonary disease (COPD) is a multifactorial disorder, therefore, airflow limitation alone does not reflect the full burden of COPD. In Hungary, no national data are available yet about the results obtained with the different COPD questionnaires in daily patient care, and about diffic...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章

    doi:10.1556/650.2020.31661

    authors: Tamási L,Speer G,AMULET vizsgálóorvosai: .

    更新日期:2020-02-01 00:00:00

  • [Insertion of a gliding prosthesis of Hungarian manufacture, without the use of adhesives].

    abstract::The author report on the first implantations of gliding prosthesis performed without sticking in Hungary. Gliding prostheses of Hungarian product were used. Thirty operations have been carried out so far. The technique and results will be discussed in a later publication on the basis of higher number of cases. ...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章

    doi:

    authors: Hulln I

    更新日期:1989-07-16 00:00:00

  • [The effect of the blood pressure lowering effect of bopindolol (Sandonorm) in patients with mild and moderate hypertension].

    abstract::The effect of a once a day dose one or one and a half mg bopindolol on mild to moderate hypertensive patients was studied. During the treatment the changes in blood pressure, heart rate, serum triglyceride, cholesterin, HDL cholesterin were examined. The drug was given as monotherapy to 9 previously untreated patients...

    journal_title:Orvosi hetilap

    pub_type: 临床试验,杂志文章

    doi:

    authors: Zsáry A,Káldi N,Kállay K

    更新日期:1991-09-29 00:00:00

  • [Do we use digitalis properly in the management of elderly patients suffering from the signs of chronic heart failure?].

    abstract:INTRODUCTION:The indications of digoxin therapy has been significantly narrowed and also the effective target therapeutic blood level has been decreased (0.9 micromol/L) compared to the previously desired one. OBJECTIVE:In this retrospective trial the data of 60 consecutive patients over 65 years (25 male, 35 female, ...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章

    doi:

    authors: Erdei F,Vendrey R,Dani G,Kovács E,Márk L,Katona A

    更新日期:2006-10-15 00:00:00

  • [Characteristics, diagnosis and treatment of the most common bacterial diseases of the oral cavity].

    abstract::Billions of microorganisms can be found in the oral cavity, from which bacteria are the most frequent. More than 600 bacterial species can be isolated. Most of them are harmless, moreover, some species prove themselves to be specifically useful. However, in the case of a weakened immune status or inappropriate oral hy...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章,评审

    doi:10.1556/650.2019.31377

    authors: Mensch K,Nagy G,Nagy Á,Bródy A

    更新日期:2019-05-01 00:00:00

  • [Modern diagnostics in breast cancer: nuclear medicine techniques].

    abstract::The authors discuss the role of nuclear medicine techniques in the modern diagnostics of breast cancer, including the methods currently used in Hungary and the future possibilities. ...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章,评审

    doi:10.1556/OH.2012.29256

    authors: Borbély K,Sinkovics I,Madaras B,Horváth Z,Láng I,Kásler M

    更新日期:2012-01-08 00:00:00

  • [Impact of microRNAs on molecular epidemiology].

    abstract::Cancer research concerning short non-coding RNA sequences and functionally linked to RNA interference (RNAi) have reached explosive breakthrough in the past decade. Molecular technology applies microRNA in extremely wide spectrum from molecular tumor prediction, diagnostics, progression monitoring and prevention. Func...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章,评审

    doi:10.1556/OH.2011.29066

    authors: Gocze K,Gombos K,Pajkos G,Magda I,Ember A,Juhász K,Patczai B,Ember I

    更新日期:2011-04-17 00:00:00

  • [Skin as an immune system].

    abstract::Skin consists of three structurally and functionally distinct compartments containing resident and nonresident cells. These cells cooperative with humoral pathway of immune system in defence of healthy skin. Resident and nonresident cells are able to initiate inflammatory or immune processes of skin, although intersti...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章

    doi:

    authors: Hunyadi J,Szabó I,Simon M Jr

    更新日期:1994-12-11 00:00:00

  • [Maximizing nodal yield while minimizing surgical morbidity: the appropriate neck dissection].

    abstract::Cervical regional lymphadenectomy, also known as neck dissection, is a fundamental procedure in head and neck surgery. Its evolution over 110 years resulted in a great deal of confusion in the literature and in clinical practice, due to the heterogenicity in training, classification and surgical techniques, which make...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章,评审

    doi:10.1556/650.2016.30570

    authors: Háromi I,Gerlinger I,Lujber L,Lőrincz BB

    更新日期:2016-11-01 00:00:00

  • [Three-dimensional speckle-tracking echocardiography -- a further step in non-invasive three-dimensional cardiac imaging].

    abstract::Three-dimensional speckle-tracking echocardiography is a new cardiac imaging methodology, which allows three-dimensional non-invasive evaluation of the myocardial mechanics. The aim of this review is to present this new tool emphasizing its diagnostic potentials and demonstrating its limitations, as well. ...

    journal_title:Orvosi hetilap

    pub_type: 杂志文章,评审

    doi:10.1556/OH.2012.29466

    authors: Nemes A,Kalapos A,Domsik P,Forster T

    更新日期:2012-10-07 00:00:00