Abstract:
:Different myosin isoenzymes of pig and human atrium and ventricle and rat ventricle were characterized by two approaches: pyrophosphate polyacrylamide gel electrophoresis (PP-PAGE) and analysis of the myosin P light chains by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). We further investigated the relation between atrial and ventricular myosin isoenzymes of human, pig, and rat, and the maximum (unloaded) shortening velocity (Vmax) and the Ca2+ sensitivity of chemically skinned fibers of the same species. The myosin isoenzymes of both human and pig atrium comigrated in the PP-PAGE with rat V2 isomyosin, whereas the ventricle of human and pig comigrated with rat V3. In both human and pig ventricle, a myosin P light chain polymorphism exists (two phosphorylatable P light chains with the same molecular weight but different isoelectric points). In contrast, we found no P light chain polymorphism in the atrium of human and pig and in the ventricle of rat (one phosphorylatable P light chain only). A correlation exists between Vmax, Ca2+ sensitivity, and atrium- and ventricle-specific myosin isoenzymes of human and pig. Vmax was determined by the slack-test method. Plots of delta l versus delta t of atrial and ventricular skinned fibers were well fitted by a single straight line up to delta l = 15% and delta l = 13%, respectively. Vmax of skinned ventricular fibers was lower than Vmax of skinned atrial fibers in both human and pig. Ca2+ sensitivity of skinned fibers of ventricle, however, was higher than Ca2+ sensitivity of atrial skinned fibers in both human and pig.
journal_name
Circ Resjournal_title
Circulation researchauthors
Morano I,Arndt H,Gärtner C,Rüegg JCdoi
10.1161/01.res.62.3.632subject
Has Abstractpub_date
1988-03-01 00:00:00pages
632-9issue
3eissn
0009-7330issn
1524-4571journal_volume
62pub_type
杂志文章abstract:RATIONALE:Elevations in C-reactive protein (CRP) are associated with increased cardiovascular disease risk and endothelial dysfunction. CRP antagonizes endothelial nitric oxide synthase (eNOS) through processes mediated by the IgG receptor Fcγ receptor IIB (FcγRIIB), its immunoreceptor tyrosine-based inhibitory motif, ...
journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.111.254573
更新日期:2011-10-28 00:00:00
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pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.116.308750
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pub_type: 杂志文章
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更新日期:2008-08-15 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.71.6.1482
更新日期:1992-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-05-23 00:00:00
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pub_type: 杂志文章
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更新日期:1985-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2020-07-03 00:00:00
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pub_type: 杂志文章,评审
doi:10.1161/CIRCRESAHA.116.307898
更新日期:2017-03-31 00:00:00
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pub_type: 杂志文章
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更新日期:2011-12-09 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:1991-02-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.54.1.65
更新日期:1984-01-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:2004-04-16 00:00:00
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pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.116.310272
更新日期:2017-03-31 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.75.5.932
更新日期:1994-11-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:2008-07-18 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2003-08-22 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.119.315037
更新日期:2019-08-16 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:2015-02-13 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章,多中心研究
doi:10.1161/CIRCRESAHA.114.305951
更新日期:2015-09-11 00:00:00
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更新日期:2003-02-07 00:00:00
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pub_type: 杂志文章
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更新日期:2015-07-03 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.66.4.1133
更新日期:1990-04-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.115.304457
更新日期:2015-01-02 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.RES.0000229267.77982.0d
更新日期:2006-06-23 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/CIRCRESAHA.116.309322
更新日期:2017-01-20 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.41.4.4
更新日期:1977-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1987-09-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:1991-10-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
doi:10.1161/01.res.80.1.37
更新日期:1997-01-01 00:00:00
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journal_title:Circulation research
pub_type: 杂志文章
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更新日期:1989-07-01 00:00:00