Abstract：
PURPOSE:Transcriptional silencing of tumor suppressor genes associated with DNA hypermethylation has been known as a hallmark of human cancer. In this study, we revealed that a local anesthetic, procaine (PCA), possessed growth-inhibitory and demethylating effect on human hepatoma cells in vitro and in vivo. METHODS:The viability of PCA-treated cells with or without trichostatin A (TSA) was investigated. To clarify the mechanism of the antiproliferating effect of PCA, TUNEL assay, FACS analysis, and morphological observation of PCA-treated cells were performed. The expression levels and epigenetic alterations of 4 genes inactivated by DNA hypermethylation in hepatocellular carcinoma (HCC) were examined in hepatoma cells with or without PCA treatment. The growth-inhibitory and demethylating effect of PCA in vivo was tested in nude mice bearing xenograft. RESULTS:The viability of HLE, HuH7, and HuH6 cells was significantly decreased by PCA treatment. In these cells, the combination treatment with TSA and PCA exhibited stronger reduction of the viability. Inhibition of S/G2/M transition, morphological changes such as vacuolation and no increase in apoptosis rate were observed in the PCA-treated HLE cells. All the genes transcriptionally suppressed by DNA hypermethylation were demethylated and reactivated with PCA treatment. PCA treatment led to partial demethylation and significant reduction in tumor volume in vivo. CONCLUSIONS:These data indicated that PCA had growth-inhibitory and demethylating effects on human hepatoma cells in vitro and in vivo. PCA may be a candidate agent for future therapies for HCC.
journal_name
Hepatol Intjournal_title
Hepatology internationalauthors
Tada M,Imazeki F,Fukai K,Sakamoto A,Arai M,Mikata R,Tokuhisa T,Yokosuka Odoi
10.1007/s12072-007-9014-5subject
Has Abstractpub_date
2007-09-01 00:00:00pages
355-64issue
3eissn
1936-0533issn
1936-0541journal_volume
1pub_type
杂志文章abstract：BACKGROUND/PURPOSE:In Japan, there is a growing population of patients with chronic hepatitis C virus (HCV) infection who failed a direct-acting antiviral (DAA)-based regimen. In this Phase 3 study, we evaluated sofosbuvir-velpatasvir plus ribavirin in Japanese patients with genotype 1 or 2 HCV infection who previously...
journal_title：Hepatology international
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1007/s12072-018-9878-6
更新日期：2018-07-01 00:00:00
abstract：PURPOSE:Interferon-induced graft dysfunction (IGD) is a poorly defined, unrecognized, but potentially serious condition for patients receiving antiviral drugs after liver transplantation for hepatitis C. METHODS:We evaluated the characteristics of 80 patients who received pegylated interferon-based antiviral treatment...
journal_title：Hepatology international
pub_type： 杂志文章
doi：10.1007/s12072-013-9496-2
更新日期：2014-04-01 00:00:00
abstract：:The presence of hepatic encephalopathy (HE) within 4 weeks is part of the criteria for defining acute-on-chronic liver failure (ACLF). The pathophysiology of HE is complex, and hyperammonemia and cerebral hemodynamic dysfunction appear to be central in the pathogenesis of encephalopathy. Recent data also suggest that ...
journal_title：Hepatology international
pub_type： 杂志文章,评审
doi：10.1007/s12072-015-9626-0
更新日期：2015-10-01 00:00:00
abstract：:An ultrasound (US) examination is a common noninvasive technique widely applied for diagnosis of a variety of diseases. Based on the rapid development of US equipment, many US images have been accumulated and are now available and ready for the preparation of a database for the development of computer-aided US diagnos...
journal_title：Hepatology international
pub_type： 杂志文章,评审
doi：10.1007/s12072-019-09937-4
更新日期：2019-07-01 00:00:00
abstract：:The cancer stem cell (CSC) hypothesis has been disproved in many cancers. CSCs may exist in blood cancer, while many epithelial cancers may not have CSCs but tumor-initiating cells (TICs). Several independent studies have provided strong evidence for existence of CSCs in brain, skin, and colon cancers (Mani et al. in ...
journal_title：Hepatology international
pub_type： 杂志文章
doi：10.1007/s12072-016-9777-7
更新日期：2017-03-01 00:00:00
abstract：GOALS:The aim of our study is to assess the prevalence of nonalcoholic fatty liver disease (NAFLD) and advanced hepatic fibrosis in patients with type 1 diabetes (T1D) using simple noninvasive scores. BACKGROUND:There is paucity of data on the prevalence of NAFLD in T1D. Moreover, T1D could be a risk factor for advanc...
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pub_type： 杂志文章
doi：10.1007/s12072-017-9840-z
更新日期：2018-01-01 00:00:00
abstract：PURPOSE:To evaluate the recent topics of Ballloon-occluded retrograde trasnvenous obliteration(B-RTO). METHOD:We overviewed the recent scientific papers regarding B-RTO. RESULT:B-RTO is a treatment method for occluding varices retrogradely using a sclerosing agent under balloon occlusion of a major draining vein. It ...
journal_title：Hepatology international
pub_type： 杂志文章,评审
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更新日期：2018-02-01 00:00:00
abstract：BACKGROUND:Early prediction of lamivudine (LAM) response by individualized monitoring of serum HBV DNA like roadmap concept, and investigation of the outcome after LAM discontinuation in renal transplant recipients (RTRs) with chronic hepatitis B (CHB). METHODS:We conducted a study on 19 RTRs with HBV infection receiv...
journal_title：Hepatology international
pub_type： 杂志文章
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更新日期：2012-04-01 00:00:00
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journal_title：Hepatology international
pub_type： 杂志文章
doi：10.1007/s12072-008-9070-5
更新日期：2008-12-01 00:00:00
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journal_title：Hepatology international
pub_type： 杂志文章,meta分析
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更新日期：2020-03-01 00:00:00
abstract：BACKGROUND:The aim of our study was to explore how C1QTNF1-AS1 regulated miR-221-3p/SOCS3 axis in human hepatocellular carcinoma (HCC). METHODS:Differentially expressed lncRNAs and genes were examined via RNA-seq. GO analysis and KEGG pathway enrichment analysis were carried out based on the function of dys-regulated ...
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pub_type： 杂志文章
doi：10.1007/s12072-019-09944-5
更新日期：2019-05-01 00:00:00
abstract：BACKGROUND/PURPOSE:Gut microbiota has been associated with liver cirrhosis and, possibly, hepatic encephalopathy. However, only a few studies have examined the link between mucosa-associated microbiota (MAM) and minimal hepatic encephalopathy (MHE). Our aim was to investigate this relationship. METHODS:Twenty-four pat...
journal_title：Hepatology international
pub_type： 杂志文章
doi：10.1007/s12072-019-09963-2
更新日期：2019-07-01 00:00:00
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journal_title：Hepatology international
pub_type： 杂志文章
doi：10.1007/s12072-012-9370-7
更新日期：2013-06-01 00:00:00
abstract：:More than 170 million people worldwide are infected with hepatitis C virus (HCV). A characteristic of this virus is a high tendency toward chronic infection. Several factors affect the viral outcome after infection. Among them, HCV-specific CD4 T cells are thought to play a crucial role in controlling viremia. Cumulat...
journal_title：Hepatology international
pub_type： 杂志文章
doi：10.1007/s12072-011-9321-8
更新日期：2012-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s12072-013-9462-z
更新日期：2013-10-01 00:00:00
abstract：BACKGROUND:Although arterial stiffness has been reported to be associated with nonalcoholic fatty liver disease (NAFLD), previous studies have relied only on noninvasive assessments for the diagnosis of NAFLD. This study attempted to investigate the association of arterial stiffness with the histological severity of NA...
journal_title：Hepatology international
pub_type： 杂志文章
doi：10.1007/s12072-020-10108-z
更新日期：2020-12-01 00:00:00
abstract：PURPOSE:The diagnostic potential of hepatic stellate cells (HSCs) and γδT cells for patients with hepatocellular carcinoma (HCC) and their synergistic contributions to the prognosis of these patients have not yet been investigated. The aim of this study was to elucidate the prognostic value of these cells in HCC. METH...
journal_title：Hepatology international
pub_type： 杂志文章
doi：10.1007/s12072-019-09969-w
更新日期：2019-09-01 00:00:00
abstract：PURPOSE:We investigated whether the combined presence and evolution of hepatitis B virus (HBV) mutant strains in the hepatitis B e antigen (HBeAg)-positive status can predict clinical outcomes after HBeAg seroconversion. METHODS:One hundred and eighty-six patients with spontaneous HBeAg seroconversion were enrolled in...
journal_title：Hepatology international
pub_type： 杂志文章
doi：10.1007/s12072-012-9381-4
更新日期：2013-06-01 00:00:00
abstract：BACKGROUND:Sorafenib significantly improves survival in patients with advanced hepatocellular carcinoma (HCC). This phase IV study assessed sorafenib efficacy/safety in Taiwanese patients with advanced HCC and Child-Pugh A status. METHODS:All patients received 400 mg sorafenib BID. Safety, efficacy, sorafenib pharmaco...
journal_title：Hepatology international
pub_type： 杂志文章,多中心研究,随机对照试验
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更新日期：2017-03-01 00:00:00
abstract：:In patients with chronic hepatitis B (CHB), liver fibrosis assessment is essential not only for determining prognosis but also for identifying patients who should receive treatment. Liver biopsy is limited by its invasiveness and sampling error. To explore effective non-invasive methods for liver fibrosis assessment, ...
journal_title：Hepatology international
pub_type： 杂志文章
doi：10.1007/s12072-013-9439-y
更新日期：2013-06-01 00:00:00
abstract：:Primary sclerosing cholangitis (PSC) is a rare cholestatic liver disease, characterized by multiple strictures and dilatations of the intra- and extrahepatic bile ducts, leading to progressive liver fibrosis, in 10-15% cholangiocarcinoma, and ultimately end-stage liver disease. The pathogenesis is poorly understood, b...
journal_title：Hepatology international
pub_type： 杂志文章,评审
doi：10.1007/s12072-020-10118-x
更新日期：2020-12-30 00:00:00
abstract：BACKGROUND AND OBJECT:The risk of occult HBV infection (OBI) in children whose mothers are HBV carriers has received more widespread attention, but there were few reports to focus on the children with HBsAg-positive parents. In this study, we aimed to investigate the prevalence of OBI in immunized children with HBsAg-p...
journal_title：Hepatology international
pub_type： 杂志文章
doi：10.1007/s12072-020-10055-9
更新日期：2020-07-01 00:00:00
abstract：BACKGROUND:The life cycle of hepatitis C virus (HCV) is tightly associated with host lipoprotein metabolic pathways. Apolipoprotein is present on the outer surface of lipoprotein particles and plays an important role in lipoprotein metabolism. We aimed to elucidate the influence of chronic HCV infection on serum apolip...
journal_title：Hepatology international
pub_type： 杂志文章
doi：10.1007/s12072-014-9572-2
更新日期：2014-10-01 00:00:00
abstract：PURPOSE:Ethanol binge augments liver injury after chronic ethanol consumption in humans, but the mechanism behind the enhanced liver injury by ethanol binge is not known. In this study we used a clinically relevant rat model in which liver injury is amplified by binge after chronic ethanol treatment and investigated th...
journal_title：Hepatology international
pub_type： 杂志文章
doi：10.1007/s12072-014-9546-4
更新日期：2014-09-01 00:00:00
abstract：PURPOSE:Case reports and small case series have reported a high rate of complications associated with chest tube placement for hepatic hydrothorax. We describe the in-hospital and 3-month outcomes of patients who have had this procedure. METHODS:A retrospective medical record review was performed of all patients admit...
journal_title：Hepatology international
pub_type： 杂志文章
doi：10.1007/s12072-009-9136-z
更新日期：2009-12-01 00:00:00
abstract：OBJECTIVE:Cholecystectomy is routinely performed at most transplant centers during living donor liver transplantation (LDLT). This study was performed to evaluate the feasibility of liver graft procurement with donor gallbladder preservation in LDLT. METHODS:Eighty-nine LDLTs (from June 2006 to Dec 2012) were retrospe...
journal_title：Hepatology international
pub_type： 杂志文章
doi：10.1007/s12072-015-9628-y
更新日期：2015-10-01 00:00:00
abstract：PURPOSE:To evaluate the sequence variations in the enhancer II (EnhII)/basal core promotor (BCP)/precore (PC) and X genes of hepatitis B virus (HBV) in Thai patients with hepatocellular carcinoma (HCC) by conducting a cross-sectional case-control study. METHODS:As much as 60 patients with HCC and 60 patients without H...
journal_title：Hepatology international
pub_type： 杂志文章
doi：10.1007/s12072-010-9197-z
更新日期：2010-07-31 00:00:00
abstract：PURPOSE:Either combination treatment or monotherapy using agents with a high genetic barrier are recommended for hepatitis B e antigen (HBeAg)-negative chronic hepatitis B (CHB). The aim of this study was to compare effect of naïve HBeAg-negative CHB patients with either de novo combination of lamivudine (LAM) and adef...
journal_title：Hepatology international
pub_type： 杂志文章
doi：10.1007/s12072-010-9243-x
更新日期：2011-06-01 00:00:00
abstract：:Actinomycotic hepatic abscess was diagnosed in a 46-year-old male driver from Ukraine presenting with the symptoms of malaise, loss of appetite, upper right quadrant pain, weight loss, and night sweats which had been present for last 2 months. Computed tomography (CT) of the abdomen revealed a hypodense mass in the le...
journal_title：Hepatology international
pub_type： 杂志文章
doi：10.1007/s12072-007-9032-3
更新日期：2008-03-01 00:00:00
abstract：:Patients with chronic hepatitis C virus (HCV) infection experience a range of symptoms including depression, fatigue and neurocognitive deficits, impairing quality of life. Depression, in particular, may be reactive to increased psychosocial stress, and the physical symptoms of advanced HCV or associated comorbidities...
journal_title：Hepatology international
pub_type： 杂志文章,评审
doi：10.1007/s12072-018-9879-5
更新日期：2018-07-01 00:00:00
