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Production of a biopesticide on host and Non-Host serine protease inhibitors for red palm weevil in palm trees.

Abstract：

:Serine proteases are essential metabolic enzymes in the midgut of many pests, including the red palm weevil (RPW), Rhynchophorus ferrugineus Olivier, which has a significant impact economically, environmentally and socially worldwide especially in the middle east. Some methods have been used to manage this pest such as trapping of RPW with pheromones, chemicals, and X-rays. However, these methods are costly, not effective and negatively impact the human. The main objective of this study is to contribute to the discovery of an eco-friendly pesticide to eradicate this infection by using serine protease inhibitors (SPIs) extracted from different parts of plant resources. In this research, both in vitro and in vivo effects of SPIs activity against RPW were examined. The protease inhibitors (PIs) activity was recorded in the crude extract that was isolated from the date's kernel (DKE), host and Calotropis latex (CLE), non-host. These PIs were partially purified by ammonium sulfate precipitation. The midgut tissue of RPW was extracted and analyzed for protases activity assay. PIs assays were consistent with the increased in the inhibitory activity against the midgut proteases after treatment with a DKE and CLE. The reduction of gut proteases by DKE solution and CLE was 39%, 18%, respectively. Partially purified DKE showed the most prominent inhibition pattern of protease activity of the gut extract. While, latex exhibited acute toxicity, imparting the least LC50 (5.132 mg/mL) against RPW larvae. Taken together, these findings provide evidence for the hypothesis that SPIs activity may play an important role in enhancing the mortality of RPW and relieving the toxicity of insecticide in palm trees.

journal_name

Saudi J Biol Sci

journal_title

Saudi journal of biological sciences

authors

Orfali R,Binsuwaileh A,Abu Al-Ala'a H,Bane-Gamea S,Zaidan N,Abdelazim M,Alhasan Ismael M,Perveen S,Majrashi N,Alluhayb K,Orfali RS

doi

10.1016/j.sjbs.2020.06.048

subject

Has Abstract

pub_date

2020-10-01 00:00:00

pages

2803-2808

issue

eissn

1319-562X

issn

2213-7106

pii

S1319-562X(20)30288-6

journal_volume

pub_type

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Production of a biopesticide on host and Non-Host serine protease inhibitors for red palm weevil in palm trees.