Abstract：
:Urinary tract infections are second most important diseases worldwide due to the increased amount of antibiotic resistant microbes. Among the Gram negative bacteria, P. mirabilis is the dominant biofilm producer in urinary tract infections next to E. coli. Biofilm is a process that produced self-matrix of more virulence pathogens on colloidal surfaces. Based on the above fact, this study was concentrated to inhibit the P. mirabilis biofilm formation by various in-vitro experiments. In the current study, the anti-biofilm effect of essential oils was recovered from the medicinal plant of Solanum nigrum, and confirmed the available essential oils by liquid chromatography-mass spectroscopy analysis. The excellent anti-microbial activity and minimum biofilm inhibition concentration of the essential oils against P. mirabilis was indicated at 200 µg/mL. The absence of viability and altered exopolysaccharide structure of treated cells were showed by biofilm metabolic assay and phenol-sulphuric acid method. The fluorescence differentiation of P. mirabilis treated cells was showed with more damages by confocal laser scanning electron microscope. Further, more morphological changes of essential oils treated cells were differentiated from normal cells by scanning electron microscope. Altogether, the results were reported that the S. nigrum essential oils have anti-biofilm ability.
journal_name
Saudi J Biol Scijournal_title
Saudi journal of biological sciencesauthors
Khaled JM,Alyahya SA,Chenthis Kanisha C,Alharbi NS,Kadaikunnan S,Ramachandran G,Alanzi KF,Rajivgandhi G,Vimala R,Manoharan Ndoi
10.1016/j.sjbs.2020.10.003subject
Has Abstractpub_date
2021-01-01 00:00:00pages
302-309issue
1eissn
1319-562Xissn
2213-7106pii
S1319-562X(20)30483-6journal_volume
28pub_type
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