Investigation of the effect of nitrogen on severity of Fusarium head blight in barley.

Abstract:

:The effect of nitrogen on Fusarium Head Blight (FHB) in a susceptible barley cultivar was investigated using gel-based proteomics. Barley grown with either 15 or 100kgha(-1)N fertilizer was inoculated with Fusarium graminearum (Fg). The storage protein fraction did not change significantly in response either to N level or Fg, whereas eighty protein spots in the water-soluble albumin fraction increased and 108 spots decreased more than two-fold in intensity in response to Fg. Spots with greater intensity in infected plants contained fungal proteins (9 spots) and proteolytic fragments of plant proteins (65 spots). Identified fungal proteins included two superoxide dismutases, L-xylulose reductase in two spots, peptidyl prolyl cis-trans isomerase and triosephosphate isomerase, and proteins of unknown function. Spots decreasing in intensity in response to Fg contained plant proteins possibly degraded by fungal proteases. Greater spot volume changes occurred in response to Fg in plants grown with low nitrogen, although proteomes of uninfected plants were similar for both treatments. Correlation of proteome changes with measurement of Fusarium-damaged kernels, fungal biomass and mycotoxin levels indicated that increased Fusarium infection occurred in barley with low N and suggests control of N fertilization as a possible way to minimise FHB in barley.

journal_name

J Proteomics

journal_title

Journal of proteomics

authors

Yang F,Jensen JD,Spliid NH,Svensson B,Jacobsen S,Jørgensen LN,Jørgensen HJ,Collinge DB,Finnie C

doi

10.1016/j.jprot.2009.10.010

subject

Has Abstract

pub_date

2010-02-10 00:00:00

pages

743-52

issue

4

eissn

1874-3919

issn

1876-7737

pii

S1874-3919(09)00308-X

journal_volume

73

pub_type

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