Abstract:
:Toxoplasma gondii is a ubiquitous, obligate intracellular parasite capable of crossing the placental barrier and causing spontaneous abortion, preterm labor, or significant disease in the surviving neonate. To better understand molecular mechanisms underlying abnormal pregnancy outcomes caused by T. gondii, placental proteins extracted from T. gondii-infected and -uninfected mice were comparatively analyzed using label-free liquid chromatography-tandem mass spectrometry. Significant difference was observed in the expression of 58 out of 792 proteins in infected placentas (p<0.05) compared with that in uninfected placentas. Quantitative real-time polymerase chain reaction, western blotting, and immunohistochemical staining were used to validate the results of the proteomic analysis. Some placental proteins differentially expressed in infected and uninfected mice were found to be associated with several different biological processes of pregnancy, particularly with trophoblast invasion and placental development. The results provide possible novel insights into the molecular mechanisms for abnormal pregnancy outcomes associated with T. gondii infection. SIGNIFICANCE:In order to further explore the mechanisms of abnormal pregnant outcomes caused by T. gondii infection, we first applied label-free proteomic technology to analyze the differentially expressed host placental proteins with T. gondii infection. The results showed that some differential proteins are associated with trophoblast invasion and placenta development. The findings provide a systemic view of the altered placental proteins and help to declare the molecular mechanisms of abnormal pregnancy outcomes caused by T. gondii infection.
journal_name
J Proteomicsjournal_title
Journal of proteomicsauthors
Jiao F,Zhang D,Jiang M,Mi J,Liu X,Zhang H,Hu Z,Xu X,Hu Xdoi
10.1016/j.jprot.2016.08.013subject
Has Abstractpub_date
2017-01-06 00:00:00pages
31-39eissn
1874-3919issn
1876-7737pii
S1874-3919(16)30388-8journal_volume
150pub_type
杂志文章abstract:UNLABELLED:Dental cementum (DC) is a bone-like tissue covering the tooth root and responsible for attaching the tooth to the alveolar bone (AB) via the periodontal ligament (PDL). Studies have unsuccessfully tried to identify factors specific to DC versus AB, in an effort to better understand DC development and regener...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2013.08.016
更新日期:2013-10-08 00:00:00
abstract:UNLABELLED:Increasing atmospheric carbon dioxide results in ocean acidification and warming, significantly impacting marine invertebrate larvae development. We investigated how ocean acidification in combination with warming affected D-veliger larvae of the Pacific oyster Crassostrea gigas. Larvae were reared for 40h u...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2015.12.001
更新日期:2016-03-01 00:00:00
abstract::Membrane proteomics offers unprecedented possibilities to compare protein expression in health and disease leading potentially to the identification of markers, of targets for therapeutics and to a better understanding of disease mechanisms. From transfusion medicine to infectious diseases, from cardiovascular affecti...
journal_title:Journal of proteomics
pub_type: 杂志文章,评审
doi:10.1016/j.jprot.2009.07.004
更新日期:2010-01-03 00:00:00
abstract::Protein phosphorylation plays a pivotal role in the regulation of many cellular events; increasing evidences indicate that this post-translational modification is involved in plant response to various abiotic and biotic stresses. Since phosphorylated proteins may be present at low abundance, enrichment methods are gen...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2011.05.016
更新日期:2011-09-06 00:00:00
abstract::Several proteomics studies have been conducted to identify new cerebrospinal fluid (CSF) biomarkers in Multiple Sclerosis (MuS). However, the complexity of CSF and its invasive collection, limits its use. Therefore, the goal of biomarker research in MuS is to identify novel distinctive targets in CSF or in easily acce...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2019.103403
更新日期:2019-07-30 00:00:00
abstract::Chronic Schistosoma mansoni infection can present as a moderate or severe disease, termed intestinal or hepatosplenic schistosomiasis, respectively. Similarly, either moderate splenomegaly or hypersplenomegaly syndrome develops in CBA/J mice by 20weeks of infection and is similar to intestinal or hepatosplenic schisto...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2011.12.025
更新日期:2012-03-16 00:00:00
abstract::MALDI Mass Spectrometry Imaging has shown important potential for molecular classification and pathology marker discovery. Protein markers identification is therefore of prime importance. Direct structural analysis from tissue sections has shown limitations for protein identification because of the high degree of comp...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2012.11.025
更新日期:2013-02-21 00:00:00
abstract::A synopsis of in-cell NMR spectroscopic approaches to study interaction proteomics in prokaryotic and eukaryotic cells is presented. We describe the use of in-cell NMR spectroscopy to resolve high resolution protein structures, discuss methodologies for determining and analyzing high and low affinity protein-target st...
journal_title:Journal of proteomics
pub_type: 杂志文章,评审
doi:10.1016/j.jprot.2018.02.006
更新日期:2019-01-16 00:00:00
abstract::Intensive methodological developments and technology innovation have been devoted to protein-protein interaction studies over 20years. Genetic indirect assays and sophisticated large scale biochemical analyses have jointly contributed to the elucidation of protein-protein interactions, still with a lot of drawbacks de...
journal_title:Journal of proteomics
pub_type: 历史文章,杂志文章,评审
doi:10.1016/j.jprot.2014.03.038
更新日期:2014-07-31 00:00:00
abstract::Sheep have a variable ability to resist gastrointestinal nematode infection, but the key factors mediating this response are poorly defined. Here we report the first large-scale application of quantitative proteomic technologies to define proteins that are differentially abundant between sheep selectively bred to have...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2012.01.016
更新日期:2012-04-03 00:00:00
abstract::Ionising radiation (IR) affects cellular and tissue function. However, the biological effects and interactions induced by IR are unclear. The aim of this study was to decipher the proteomic patterns that influence these pathways. The proteomes of Arabidopsis thaliana roots and rosettes were analysed in response to sub...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2011.03.025
更新日期:2011-08-12 00:00:00
abstract::Growth hormone (GH) promotes longitudinal growth and bone mineralization. In this study, a proteomic approach was used to analyze the association between serum protein expression pattern and height-adjusted bone mineralization in short prepubertal children receiving GH treatment. Patterns of protein expression were co...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2010.08.007
更新日期:2011-01-01 00:00:00
abstract::Radiation-induced colorectal fibrosis (RICF) after pelvic radiotherapy can lead to stenosis and affect patients' quality of life. However, to date, few effective prevention and treatment methods for RICF have been implemented in clinical practice. Therefore, there is a strong need to investigate the molecular events u...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2020.103801
更新日期:2020-07-15 00:00:00
abstract::Quantitative proteomic comparisons require a sufficient number of samples to reach an acceptable level of significance. But 2D gel electrophoresis commonly results in incomplete data sets due to spots with missing values reducing thereby the number of parallel measurements for individual proteins. Here we investigated...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2011.12.019
更新日期:2012-03-16 00:00:00
abstract::Dystrobrevin is a dystrophin-related component of the dystrophin-associated protein complex (DAPC). Using alpha-dystrobrevin as indicator, we aimed to elucidate the interaction network of the DAPC with other proteins during apoptosis of promyelocytic HL-60 cells. The precise role(s) of DBs are not known, but we and ot...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2012.03.041
更新日期:2012-06-18 00:00:00
abstract::The oral mucosal pellicle is a thin lubricating layer generated by the binding of saliva proteins on epithelial oral cells. The protein composition of this biological structure has been to date studied by targeted analyses of specific salivary proteins. In order to perform a more exhaustive proteome characterization o...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2020.103797
更新日期:2020-06-30 00:00:00
abstract::We aimed to develop prognostic biomarkers for synovial sarcoma employing a proteomic approach. We examined the proteomic profile of synovial sarcoma using two-dimensional difference gel electrophoresis (2D-DIGE). We identified 20 protein spots whose intensity was statistically different (p<0.01) between a group of eig...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2011.02.033
更新日期:2011-05-16 00:00:00
abstract::Ever since antibiotics were used to help humanity battle infectious diseases, microorganisms straight away fought back. Antibiotic resistance mechanisms indeed provide microbes with possibilities to by-pass and survive the action of antibiotic drugs. Several methods have been employed to identify these microbial resis...
journal_title:Journal of proteomics
pub_type: 杂志文章,评审
doi:10.1016/j.jprot.2013.10.027
更新日期:2014-01-31 00:00:00
abstract:UNLABELLED:The 26S proteasome is a proteolytic enzyme found in both cytoplasm and nucleus. In this study, we examined the differential expression of proteasome inhibitor bortezomib-induced proteins in p53-deficient 4T1 cells. It was found that GRP78 and TCEB2 were over-expressed in response to treatment with bortezomib...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2014.09.010
更新日期:2015-01-15 00:00:00
abstract::Since its introduction mass spectrometry imaging (MSI) has proven to be a powerful tool for the localization of molecules in biological tissues. In drug discovery and development, understanding the distribution of both drug and its metabolites is of critical importance. Traditional methods suffer from a lack of spatia...
journal_title:Journal of proteomics
pub_type: 杂志文章,评审
doi:10.1016/j.jprot.2012.07.028
更新日期:2012-08-30 00:00:00
abstract::We defined the transcriptomic and proteomic profiles of rat ageing skeletal muscle using a combined cDNA array, 2D- and Blue native-PAGE approach. This was allowed to obtain an overview of the interrelated events leading to the transcriptome/proteome/mitoproteome changes likely to underlie the structural/metabolic fea...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2009.02.007
更新日期:2009-05-02 00:00:00
abstract::The process of protein phosphorylation in cells is well studied in the context of a wide range of biologic functions such as signalling, cell cycle, cell growth and differentiation, and others. In contrast, little progress has been made in the investigation of protein phosphorylation specifically in blood. Here, we fo...
journal_title:Journal of proteomics
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1016/j.jprot.2012.02.015
更新日期:2012-12-05 00:00:00
abstract::In order to identify new protein markers modified in placental diseases, high-throughput analysis of proteins in the plasma of pregnant women was carried out for normal and pathological pregnancies (Preeclampsia and/or Intra-Uterine Growth Restriction) using iTRAQ technology. We could identify 166 proteins that were m...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2009.12.014
更新日期:2010-03-10 00:00:00
abstract::Jasmonates (JAs) are important phytohormones that regulate a wide range of plant processes, including growth, development and stress responses. Jasmonate ZIM-domain (JAZ) proteins are transcriptional repressors in JA signaling. Overexpression of JAZ7 was found to confer drought tolerance in Arabidopsis thaliana (A. th...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2019.02.001
更新日期:2019-03-30 00:00:00
abstract::High-throughput technologies were used to identify venom gland toxin expression and to characterize the venom proteomes of two rear-fanged snakes, Ahaetulla prasina (Asian Green Vine Snake) and Borikenophis portoricensis (Puerto Rican Racer). Sixty-nine complete toxin-coding transcripts from 12 venom protein superfami...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2018.08.004
更新日期:2018-09-15 00:00:00
abstract:UNLABELLED:Next generation sequencing and proteomics have helped to comprehensively characterize gene expression in tick salivary glands at both the transcriptome and the proteome level. Functional data are, however, lacking. Given that tick salivary secretions are critical to the success of the tick transmission lifec...
journal_title:Journal of proteomics
pub_type: 杂志文章,评审
doi:10.1016/j.jprot.2015.07.005
更新日期:2015-10-14 00:00:00
abstract:UNLABELLED:The pathogenicity of Yersinia enterocolitica biovar 1A strains is controversial as these lack most of the known virulence factors. Acquisition of iron and presence of well-regulated iron homeostasis in bacteria represents an important virulence trait. Differential abundance of proteins was examined under iro...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2015.04.015
更新日期:2015-06-21 00:00:00
abstract::Due to innovative advancements in quantitative MS technologies, proteomics has evolved from taking mere "snapshots" of distinct proteomes in a defined state to monitoring, for instance, changes in abundance, location and/or posttranslational modification(s) of proteins under various conditions, thereby facilitating th...
journal_title:Journal of proteomics
pub_type: 杂志文章,评审
doi:10.1016/j.jprot.2008.11.008
更新日期:2009-04-13 00:00:00
abstract:UNLABELLED:The vacuole is by far the largest multifunctional organelle in fruits and plays a functional role in fruit development and fruit quality. Despite its significance, little information exists pertaining to the role of the vacuolar membrane (tonoplast) in the process of fruit senescence. In the present study, a...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2016.06.031
更新日期:2016-09-02 00:00:00
abstract::Chlamydia trachomatis is the most common bacterial sexually transmitted pathogen in the world. To identify new vaccine candidates a protein microarray was constructed by expressing the open reading frames (ORFs) from Chlamydia mouse pneumonitis (MoPn). C57BL/6, C3H/HeN and BALB/c mice were immunized either intranasall...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2012.08.017
更新日期:2012-12-21 00:00:00