Abstract:
UNLABELLED:The pathogenicity of Yersinia enterocolitica biovar 1A strains is controversial as these lack most of the known virulence factors. Acquisition of iron and presence of well-regulated iron homeostasis in bacteria represents an important virulence trait. Differential abundance of proteins was examined under iron-rich and iron-poor conditions in a clinical Y. enterocolitica biovar 1A strain IP27407. Whole cell protein profiles were analysed by 2D gel electrophoresis (2D-GE). Following statistical and MALDI-TOF MS analyses, 28 differentially abundant proteins were identified. Significant iron-responsive changes were observed in the proteins involved in iron acquisition or storage namely, hemin receptor (HemR), periplasmic Fe(2+) transport protein (Tpd), periplasmic chelated iron-binding protein (YfeA) and bacterioferritin (Bfr). Quantitative real-time PCR (qRT-PCR) of eight mRNA transcripts revalidated the differential protein abundance. In silico analysis of iron homeostasis mediated by the bacterioferritin and bacterioferritin-associated ferredoxin (Bfr-Bfd) complex suggested two pathways for the release of reserve iron which might be operating under conditions of different iron availability. The study, for the first time, showed the existence of highly competent iron homeostasis mechanisms in Y. enterocolitica biovar 1A and identified the key proteins involved thereof. Such mechanisms might have implications for the pathogenicity of Y. enterocolitica biovar 1A strains. BIOLOGICAL SIGNIFICANCE:Although, a few studies have identified the differentially abundant bacterial proteins in response to iron starvation, little information is available in this regard for Y. enterocolitica (especially, the biovar 1A strains). In the present study, differential abundance of several proteins was identified under iron-rich and iron-poor conditions by 2D-GE and MALDI-TOF/MS analysis. These included proteins which may not only be directly implicated in iron acquisition or storage but also play crucial role in cellular metabolism. Given the absence of most known virulence factors in Y. enterocolitica biovar 1A strains, demonstration of well-regulated mechanisms for efficient iron homeostasis constitutes an important observation. The proteins, as identified in the present study, provide useful insights to further unravel the potential pathogenicity of the biovar 1A strains.
journal_name
J Proteomicsjournal_title
Journal of proteomicsauthors
Kanaujia PK,Bajaj P,Kumar S,Singhal N,Virdi JSdoi
10.1016/j.jprot.2015.04.015subject
Has Abstractpub_date
2015-06-21 00:00:00pages
39-49eissn
1874-3919issn
1876-7737pii
S1874-3919(15)00196-7journal_volume
124pub_type
杂志文章abstract::Salinity is one of the major environmental constraints limiting yield of crop plants in many semi-arid and arid regions around the world. To understand responses in soybean seedling to salt stress at proteomic level, the extracted proteins from seedling leaves of salt-sensitive genotype Jackson and salt-tolerant genot...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2011.11.026
更新日期:2012-02-16 00:00:00
abstract::Chlamydia trachomatis is the most common bacterial sexually transmitted pathogen in the world. To identify new vaccine candidates a protein microarray was constructed by expressing the open reading frames (ORFs) from Chlamydia mouse pneumonitis (MoPn). C57BL/6, C3H/HeN and BALB/c mice were immunized either intranasall...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2012.08.017
更新日期:2012-12-21 00:00:00
abstract::We aimed to develop prognostic biomarkers for synovial sarcoma employing a proteomic approach. We examined the proteomic profile of synovial sarcoma using two-dimensional difference gel electrophoresis (2D-DIGE). We identified 20 protein spots whose intensity was statistically different (p<0.01) between a group of eig...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2011.02.033
更新日期:2011-05-16 00:00:00
abstract:UNLABELLED:Alterations in the sera proteins between patients with Primary Open-Angle Glaucoma (POAG), Pseudoexfoliation Glaucoma (PEXG), and healthy controls were identified through a proven approach utilizing equalization of high-abundance serum proteins with ProteoMiner™, two-dimensional fluorescent difference gel el...
journal_title:Journal of proteomics
pub_type: 临床试验,杂志文章
doi:10.1016/j.jprot.2013.12.006
更新日期:2014-02-26 00:00:00
abstract::The yeast Saccharomyces cerevisiae is able to grow under aerobic as well as anaerobic conditions. We and others previously found that transcription levels of approximately 500 genes differed more than two-fold when cells from anaerobic and aerobic conditions were compared. Here, we addressed the effect of anaerobic gr...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2008.11.012
更新日期:2009-01-30 00:00:00
abstract::Glycosylation, one of the most common post translational modifications (PTMs) of proteins, is often associated with carcinogenesis and tumor malignancy. Ovarian cancer is the sixth cause of cancer-related death in Western countries. Currently, it is treated by debulking surgery followed by chemotherapy based on paclit...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2009.11.012
更新日期:2010-03-10 00:00:00
abstract::In this study, we performed an integrated physiological and chloroplast proteome analysis of wheat seedling leaves under salt and osmotic stresses by label-free based quantitative proteomic approach. Both salt and osmotic stresses significantly increased the levels of abscisic acid and methyl jasmonate and led to dama...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2020.104097
更新日期:2021-03-15 00:00:00
abstract:UNLABELLED:Induced pluripotent stem cells (iPSCs), derived from somatic cells and functionally very similar to embryonic stem cells (ESCs), are at the center stage of intense research in regenerative medicine. We carried out the first membrane proteomic profiling of mouse iPSCs, in comparison with ESCs and adult mouse ...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2013.04.018
更新日期:2013-06-28 00:00:00
abstract::Tissues hypoxia caused by hemorrhage is a common complication in many clinical diseases. However, its pathological mechanism remains largely unknown. To partly address this issue, an ischemic-hypoxic rat model was established and the plasma proteomic and metabolic profiles were quantified and analyzed using TMT-based ...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2020.103924
更新日期:2020-09-30 00:00:00
abstract::The recognition by specific T helper cells of viral antigenic peptides complexed with HLA class II molecules exposed on the surface of antigen presenting cells is the first step of the complex cascade of immunological events that generates the protective cellular and humoral immune responses. The HLA class II-restrict...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2020.103759
更新日期:2020-06-15 00:00:00
abstract::A large series of protein pathway components have been shown to be dysregulated in Down syndrome (DS) brain. No information about pathomechanisms linked to the trisomic state can be obtained from adult DS brain, however, as neurodegeneration occurs from the fourth decade. The aim of the study was to search for protein...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2011.01.009
更新日期:2011-04-01 00:00:00
abstract::Flightin is a myosin binding phosphoprotein that originated in the ancestor to Pancrustacea ~500 MYA. In Drosophila melanogaster, flightin is essential for length determination and flexural rigidity of thick filaments. Here, we show that among 12 Drosophila species, the N-terminal region is characterized by low sequen...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2015.12.006
更新日期:2016-03-01 00:00:00
abstract::The somatic embryogenesis in conifers represents a suitable model of plant regeneration system facilitating studies of fundamental aspects of an early development as well as in vitro micropropagation. The aim of our study was to deeper understand the somatic embryogenesis in the conifer tree Pinus nigra Arn. Comparati...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2017.05.013
更新日期:2017-10-03 00:00:00
abstract::Neurodegenerative diseases often lack early and specific diagnostic and prognostic biomarkers. Many studies are focusing on the cerebrospinal fluid (CSF) proteome to identify relevant biomarkers and therapeutic targets for these disorders. An alternative approach consists in comparing proteins secreted by healthy neur...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2012.09.013
更新日期:2012-12-21 00:00:00
abstract::Fructus Rosae Roxburghii (FRR) has been considered as edible and medicinal fruit possessing antiatherosclerotic effect, but the mechanism is still unclear. HLP is material basis for AS formation. Under FRR action, TC, TG, LDL, HDL and ASI in serum were regulated to control level. Differentially expressed proteins in l...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2020.103982
更新日期:2021-01-06 00:00:00
abstract::The process of protein phosphorylation in cells is well studied in the context of a wide range of biologic functions such as signalling, cell cycle, cell growth and differentiation, and others. In contrast, little progress has been made in the investigation of protein phosphorylation specifically in blood. Here, we fo...
journal_title:Journal of proteomics
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1016/j.jprot.2012.02.015
更新日期:2012-12-05 00:00:00
abstract::Both transmission electron microscopy (TEM) and fluorescence spectrometry were used to reveal the characteristics of both subunit disassociation and recombination in apo-pig pancreas ferritin (apoPPF) in an alkaline medium ranging reversibly from pH 7.0 to 13.0. The experimental results indicated that apoPPF could be ...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2012.03.013
更新日期:2012-06-18 00:00:00
abstract::Considering that proteins are usually engaged in complex and dynamic networks of interactions to exert their activity, a way to understand proteins' functions and the molecular mechanisms in which those proteins are involved, is by studying their interactome. In this sense, this tutorial presents a simple pipeline for...
journal_title:Journal of proteomics
pub_type: 杂志文章,评审
doi:10.1016/j.jprot.2017.04.011
更新日期:2018-01-16 00:00:00
abstract::Nitric oxide is a pain signaling molecule and exerts its influence through two primary pathways: by stimulation of soluble guanylylcyclase and by direct S-nitrosylation (SNO) of target proteins. We assessed in the spinal cord the SNO-proteome with two methods, two-dimensional S-nitrosothiol difference gel electrophore...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2012.05.006
更新日期:2012-07-16 00:00:00
abstract:UNLABELLED:Fatal events derived from coronary atherosclerosis are the major cause of mortality in the developed countries. Proteomic analysis of the atherosclerotic coronary artery has been mainly carried out with whole tissue extracts, making it difficult to distinguish the alterations present in every region of the p...
journal_title:Journal of proteomics
pub_type: 临床试验,杂志文章
doi:10.1016/j.jprot.2013.01.032
更新日期:2013-04-26 00:00:00
abstract:UNLABELLED:Trichoderma reesei is the main industrial producer of lignocellulolytic enzymes, and the secretory behavior of this fungus strongly depends on the carbon sources. To gain insights into how the T. reesei adapts to various carbons and regulates enzyme production, the extra- and intracellular proteomes of T. re...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2013.06.014
更新日期:2013-08-26 00:00:00
abstract::The pollen beetle is a major pest of oilseed rape. Although various resistance mechanisms have been identified, such as kdr (mutation in the sodium channel) and metabolic resistance (CYP overexpression), other "hidden" factors also exist. Some studies have stressed the importance of epistasis as a genetic background. ...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2020.104086
更新日期:2021-02-20 00:00:00
abstract:UNLABELLED:Extracellular vesicles are produced by many pathogenic microorganisms and have varied functions that include secretion and release of microbial factors, which contribute to virulence. Very little is known about vesicle production by Gram-positive bacteria, as well as their biogenesis and release mechanisms. ...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2014.04.023
更新日期:2014-06-25 00:00:00
abstract::Non-healing fractures constitute a serious clinical problem. HIF-1α is a crucial regulator in response to hypoxia and is proven to be pivotal in bone growth; however, the mechanism still needs further research. In this study, iTRAQ was used to study the effects of two HIF-1α inducers on the expression of proteins in M...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2019.103558
更新日期:2020-01-16 00:00:00
abstract:UNLABELLED:Mass spectrometry is already a well-established protein identification tool and recent methodological and technological developments have also made possible the extraction of quantitative data of protein abundance in large-scale studies. Several strategies for absolute and relative quantitative proteomics an...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2013.02.026
更新日期:2013-12-16 00:00:00
abstract::The human papillomavirus type 16 (HPV-16) E6/E7 spliced transcripts are heterogeneously expressed in cervical carcinoma. The heterogeneity of the E6/E7 splicing profile might be in part due to the intrinsic variation of splicing factors in tumor cells. However, the splicing factors that bind the E6/E7 intron 1 (In-1) ...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2014.07.029
更新日期:2014-12-05 00:00:00
abstract:UNLABELLED:Cystic hydatid disease is an important zoonosis caused by Echinococcus granulosus infection. The expression profiles of its parasitic life stages and host-Echinococcus interactions remain to be elucidated. Here, we identified 157 adult and 1588 protoscolex proteins (1610 in all), including 1290 novel identif...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2013.04.013
更新日期:2013-06-12 00:00:00
abstract::Expanding the sequencing depth of the peptides with a statistically significant quantitative change derived from a biological stimulation is critical. Here we demonstrate that optimization of LC gradient and analytical column construction can reveal over 30,000 unique peptides and 23,000 phosphopeptides at high confid...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2017.06.013
更新日期:2017-08-08 00:00:00
abstract::High-throughput technologies were used to identify venom gland toxin expression and to characterize the venom proteomes of two rear-fanged snakes, Ahaetulla prasina (Asian Green Vine Snake) and Borikenophis portoricensis (Puerto Rican Racer). Sixty-nine complete toxin-coding transcripts from 12 venom protein superfami...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2018.08.004
更新日期:2018-09-15 00:00:00
abstract:UNLABELLED:The membrane-associated LonB protease is essential for viability in Haloferax volcanii, however, the cellular processes affected by this protease in archaea are unknown. In this study, the impact of a lon conditional mutation (down-regulation) on H. volcanii physiology was examined by comparing proteomes of ...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2015.03.016
更新日期:2015-05-21 00:00:00