Integrated physiological and chloroplast proteome analysis of wheat seedling leaves under salt and osmotic stresses.


:In this study, we performed an integrated physiological and chloroplast proteome analysis of wheat seedling leaves under salt and osmotic stresses by label-free based quantitative proteomic approach. Both salt and osmotic stresses significantly increased the levels of abscisic acid and methyl jasmonate and led to damages of chloroplast ultrastructure. Main parameters of chlorophyll fluorescence and gas exchange showed a significant decline under both stresses. Quantitative proteomic analysis identified 194 and 169 chloroplast-localized differentially accumulated proteins (DAPs) responsive to salt and osmotic stresses, respectively. The abundance of main DAPs involved in light-dependent reaction were increased under salt stress, but decreased in response to osmotic stress. On the contrary, salt stress induced a significant upregulation of the DAPs associated with Calvin cycle, transcription and translation, amino acid metabolism, carbon and nitrogen metabolism, and some of them exhibited a downregulation under osmotic stress. In particular, both treatments significantly upregulated the DAPs involved in plastoglobule development, protein folding and proteolysis, hormone and vitamin synthesis. Finally, we proposed a putative synergistic responsive network of wheat chloroplast proteome under salt and osmotic stresses, aiming to provide new insights into the underlying response and defense mechanisms of wheat chloroplast proteome in response to abiotic stresses. SIGNIFICANCE: Salt and osmotic stresses are the two most common abiotic stresses that severely affect crop growth and productivity. As the main site of photosynthesis of plant cells, the chloroplast also plays important role in plant tolerance to abiotic stress. However, the response of chloroplast proteome to salt and osmotic is still poorly understood by using the traditional two-dimensional electrophoresis (2-DE) method due to a poor resolution of chloroplast protein separation and low throughput identification of differentially accumulated proteins (DAPs). In this study, we employed label-free based quantitative proteomic approach to perform an integrated physiological and large-scale chloroplast proteome analysis of wheat seedling leaves under salt and osmotic stresses, which laid a solid foundation for future studies into the response and defense mechanisms of wheat chloroplast in response to abiotic stresses.


J Proteomics


Journal of proteomics


Zhu D,Luo F,Zou R,Liu J,Yan Y




Has Abstract


2021-03-15 00:00:00












  • Proteomics identifies molecular networks affected by tetradecylthioacetic acid and fish oil supplemented diets.

    abstract:UNLABELLED:Fish oil (FO) and tetradecylthioacetic acid (TTA) - a synthetic modified fatty acid have beneficial effects in regulating lipid metabolism. In order to dissect the mechanisms underlying the molecular action of those two fatty acids we have investigated the changes in mitochondrial protein expression in a lon...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Wrzesinski K,R León I,Kulej K,Sprenger RR,Bjørndal B,Christensen BJ,Berge RK,Jensen ON,Rogowska-Wrzesinska A

    更新日期:2013-06-12 00:00:00

  • Technical report: Targeted proteomic analysis reveals enrichment of atypical ubiquitin chains in contractile murine tissues.

    abstract::Ubiquitylation is an elaborate post-translational modification involved in all biological processes. Its pleotropic effect is driven by the ability to form complex polyubiquitin chain architectures that can influence biological functions. In this study, we optimised sample preparation and chromatographic separation of...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Heunis T,Lamoliatte F,Marín-Rubio JL,Dannoura A,Trost M

    更新日期:2020-10-30 00:00:00

  • Proteomic identification of immunodominant chlamydial antigens in a mouse model.

    abstract::Chlamydia trachomatis is the most common bacterial sexually transmitted pathogen in the world. To identify new vaccine candidates a protein microarray was constructed by expressing the open reading frames (ORFs) from Chlamydia mouse pneumonitis (MoPn). C57BL/6, C3H/HeN and BALB/c mice were immunized either intranasall...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Teng A,Cruz-Fisher MI,Cheng C,Pal S,Sun G,Ralli-Jain P,Molina DM,Felgner PL,Liang X,de la Maza LM

    更新日期:2012-12-21 00:00:00

  • Protein nitrotryptophan: formation, significance and identification.

    abstract::Reactive nitrogen species are formed during a variety of disease states and have been shown to modify several amino acids on proteins. To date, the majority of research in this area has focused on the nitration of tyrosine residues to form 3-nitrotyrosine. However, emerging evidence suggests that another modification,...

    journal_title:Journal of proteomics

    pub_type: 杂志文章,评审


    authors: Nuriel T,Hansler A,Gross SS

    更新日期:2011-10-19 00:00:00

  • Differential impacts of individual and combined exposures of deoxynivalenol and zearalenone on the HepaRG human hepatic cell proteome.

    abstract::Numerous surveys have highlighted the natural co-occurrence of deoxynivalenol (DON) and zearalenone (ZEA) mycotoxins in food and feed. Nevertheless, data regarding cellular mechanisms involved in response to their individual and simultaneous exposures are lacking. In this study, in order to analyze how low mycotoxin d...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Smith MC,Timmins-Schiffman E,Coton M,Coton E,Hymery N,Nunn BL,Madec S

    更新日期:2018-02-20 00:00:00

  • The olfactory bulb proteotype differs across frontotemporal dementia spectrum.

    abstract::Mild olfactory dysfunction has been observed in frontotemporal dementias (FTD). However, the underlying molecular mechanisms associated to this deficit are poorly understood. We applied quantitative proteomics to analyze pathological effects on the olfactory bulb (OB) from progressive supranuclear palsy (PSP) and fron...

    journal_title:Journal of proteomics

    pub_type: 临床试验,杂志文章


    authors: Lachén-Montes M,González-Morales A,Schvartz D,Zelaya MV,Ausin K,Fernández-Irigoyen J,Sánchez JC,Santamaría E

    更新日期:2019-06-15 00:00:00

  • Probing the conformational impact of detergents on the integral membrane protein LeuT by global HDX-MS.

    abstract::Neurotransmitter:sodium symporters (NSS) are integral membrane proteins (IMP), responsible for reuptake of neurotransmitters from the synaptic cleft. Due to challenges in production of mammalian NSS in their active form, the prokaryotic hydrophobic amino acid transporter, LeuT, served here as a steadfast model for elu...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Möller IR,Merkle PS,Calugareanu D,Comamala G,Schmidt SG,Loland CJ,Rand KD

    更新日期:2020-08-15 00:00:00

  • Dynamic remodeling of the interactomes of Nematostella vectensis Hsp70 isoforms under heat shock.

    abstract::Heat shock protein 70s (Hsp70s) are a highly conserved class of molecular chaperones that fold a large proportion of the proteome. Nematostella vectensis (Nv) is an estuarine sea anemone that has emerged as a model species to characterize molecular responses to physiological stressors due to its exposure to diverse, e...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Knighton LE,Nitika,Waller SJ,Strom O,Wolfgeher D,Reitzel AM,Truman AW

    更新日期:2019-08-30 00:00:00

  • The correlation between cellular size and protein expression levels--normalization for global protein profiling.

    abstract::An automated image analysis system was used for protein quantification of 1862 human proteins in 47 cancer cell lines and 12 clinical cell samples using cell microarrays and immunohistochemistry. The analysis suggests that most proteins are expressed in a cell size dependent manner, and that normalization is required ...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Lundberg E,Gry M,Oksvold P,Kononen J,Andersson-Svahn H,Pontén F,Uhlén M,Asplund A

    更新日期:2008-10-07 00:00:00

  • The Aotus nancymaae erythrocyte proteome and its importance for biomedical research.

    abstract::The Aotus nancymaae species has been of great importance in researching the biology and pathogenesis of malaria, particularly for studying Plasmodium molecules for including them in effective vaccines against such microorganism. In spite of the forgoing, there has been no report to date describing the biology of paras...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Moreno-Pérez DA,García-Valiente R,Ibarrola N,Muro A,Patarroyo MA

    更新日期:2017-01-30 00:00:00

  • Microbial symbionts affect Pisum sativum proteome and metabolome under Didymella pinodes infection.

    abstract:UNLABELLED:The long cultivation of field pea led to an enormous diversity which, however, seems to hold just little resistance against the ascochyta blight disease complex. The potential of below ground microbial symbiosis to prime the immune system of Pisum for an upcoming pathogen attack has hitherto received little ...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Desalegn G,Turetschek R,Kaul HP,Wienkoop S

    更新日期:2016-06-30 00:00:00

  • Global role of the membrane protease LonB in Archaea: Potential protease targets revealed by quantitative proteome analysis of a lonB mutant in Haloferax volcanii.

    abstract:UNLABELLED:The membrane-associated LonB protease is essential for viability in Haloferax volcanii, however, the cellular processes affected by this protease in archaea are unknown. In this study, the impact of a lon conditional mutation (down-regulation) on H. volcanii physiology was examined by comparing proteomes of ...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Cerletti M,Paggi RA,Guevara CR,Poetsch A,De Castro RE

    更新日期:2015-05-21 00:00:00

  • Identification of plasma-responsive outer membrane proteins and their vaccine potential in Edwardsiella tarda using proteomic approach.

    abstract::We have used differential sub-proteomic methodologies to detect Edwardsiella tarda outer membrane (OM) protein expression regulation during interaction with fish and human plasma, which is the critical step of the bacterial invasion internal organs via blood circulation. Seven and nine OM proteins were differentially ...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Wang C,Liu Y,Li H,Xu WJ,Zhang H,Peng XX

    更新日期:2012-02-02 00:00:00

  • Towards posttranslational modification proteome of royal jelly.

    abstract::Royal jelly (RJ) is a secretory protein from the hypopharyngeal glands of nurse honeybee workers, which contains a variety of proteins of which major royal jelly proteins (MRJPs) are some of the most important. It plays important roles both for honeybee and human. Each family of MRJP 1-5 displays a string of modified ...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Zhang L,Fang Y,Li R,Feng M,Han B,Zhou T,Li J

    更新日期:2012-09-18 00:00:00

  • Proteomics study of open biopsy samples identifies peroxiredoxin 2 as a predictive biomarker of response to induction chemotherapy in osteosarcoma.

    abstract:UNLABELLED:We attempted to identify biomarkers that would predict responsiveness of osteosarcoma (OS) to induction chemotherapy. Tumor tissues obtained by open biopsy before induction chemotherapy were investigated. On the basis of histological observations at the time of surgery and the Huvos grading system, 7 patient...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Kubota D,Mukaihara K,Yoshida A,Tsuda H,Kawai A,Kondo T

    更新日期:2013-10-08 00:00:00

  • Immunoproteomic profiling of Saccharomyces cerevisiae systemic infection in a murine model.

    abstract:UNLABELLED:Saccharomyces cerevisiae is considered a safe microorganism widely used as a dietary supplement. However, in the latest decades several cases of S. cerevisiae infections have been reported. Recent studies in a murine model of systemic infection have also revealed the virulence of some S. cerevisiae dietary s...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Hernández-Haro C,Llopis S,Molina M,Monteoliva L,Gil C

    更新日期:2015-01-01 00:00:00

  • Twenty years of protein interaction studies for biological function deciphering.

    abstract::Intensive methodological developments and technology innovation have been devoted to protein-protein interaction studies over 20years. Genetic indirect assays and sophisticated large scale biochemical analyses have jointly contributed to the elucidation of protein-protein interactions, still with a lot of drawbacks de...

    journal_title:Journal of proteomics

    pub_type: 历史文章,杂志文章,评审


    authors: Legrain P,Rain JC

    更新日期:2014-07-31 00:00:00

  • Protein profiling identified dissociations between growth hormone-mediated longitudinal growth and bone mineralization in short prepubertal children.

    abstract::Growth hormone (GH) promotes longitudinal growth and bone mineralization. In this study, a proteomic approach was used to analyze the association between serum protein expression pattern and height-adjusted bone mineralization in short prepubertal children receiving GH treatment. Patterns of protein expression were co...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Andersson B,Decker R,Nierop AF,Bosaeus I,Albertsson-Wikland K,Hellgren G

    更新日期:2011-01-01 00:00:00

  • Serological autoantibody profiling of type 1 diabetes by protein arrays.

    abstract:UNLABELLED:The need for biomarkers that illuminate the pathophysiology of type 1 diabetes (T1D), enhance early diagnosis and provide additional avenues for therapeutic intervention is well recognized in the scientific community. We conducted a proteome-scale, two-stage serological AAb screening followed by an independe...

    journal_title:Journal of proteomics

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Miersch S,Bian X,Wallstrom G,Sibani S,Logvinenko T,Wasserfall CH,Schatz D,Atkinson M,Qiu J,LaBaer J

    更新日期:2013-12-06 00:00:00

  • Secreted protein extract analyses present the plant pathogen Alternaria alternata as a suitable industrial enzyme toolbox.

    abstract::Lignocellulosic plant biomass is the most abundant carbon source in the planet, which makes it a potential substrate for biorefinery. It consists of polysaccharides and other molecules with applications in pharmaceutical, food and feed, cosmetics, paper and textile industries. The exploitation of these resources requi...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: García-Calvo L,Ullán RV,Fernández-Aguado M,García-Lino AM,Balaña-Fouce R,Barreiro C

    更新日期:2018-04-15 00:00:00

  • Identification and validation of platelet low biological variation proteins, superior to GAPDH, actin and tubulin, as tools in clinical proteomics.

    abstract:UNLABELLED:Accurate biomarker quantification requires carefully chosen normalisation procedures. When single proteins are used as loading controls (LCs), it is crucial that their expressional stability must be known. Platelets are an important biomarker source, especially for neurological diseases. We performed a syste...

    journal_title:Journal of proteomics

    pub_type: 临床试验,杂志文章


    authors: Baumgartner R,Umlauf E,Veitinger M,Guterres S,Rappold E,Babeluk R,Mitulović G,Oehler R,Zellner M

    更新日期:2013-12-06 00:00:00

  • Characterization of maize allergens - MON810 vs. its non-transgenic counterpart.

    abstract::One of the main concerns about genetically modified foods and their potential impacts on human health is that the introduction of a new/ altered gene may putatively alter the expression of others, namely endogenous allergens. We intended to evaluate, and to compare, using quantitative real time RT-PCR technique, the e...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Fonseca C,Planchon S,Renaut J,Oliveira MM,Batista R

    更新日期:2012-04-03 00:00:00

  • Peptidomic approach identifies cruzioseptins, a new family of potent antimicrobial peptides in the splendid leaf frog, Cruziohyla calcarifer.

    abstract:UNLABELLED:Phyllomedusine frogs are an extraordinary source of biologically active peptides. At least 8 families of antimicrobial peptides have been reported in this frog clade, the dermaseptins being the most diverse. By a peptidomic approach, integrating molecular cloning, Edman degradation sequencing and tandem mass...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Proaño-Bolaños C,Zhou M,Wang L,Coloma LA,Chen T,Shaw C

    更新日期:2016-09-02 00:00:00

  • Global identification of transfer RNAs by liquid chromatography-mass spectrometry (LC-MS).

    abstract::Transfer ribonucleic acid (tRNA) is the non-coding RNA that links the processes of gene transcription with protein translation. While tRNAs have, individually, been studied for many years, few approaches exist for the global identification of tRNAs at the RNA and posttranscriptional RNA levels. Previously our lab intr...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Wetzel C,Limbach PA

    更新日期:2012-06-27 00:00:00

  • N-Glycosylation pattern of recombinant human CD82 (KAI1), a tumor-associated membrane protein.

    abstract::The membrane glycoprotein CD82 (KAI1) has attracted increasing attention as a suppressor of cell migration, related tumor invasion, as well as metastasis. The glycosylation of CD82 has been shown to be involved in a correlative cell adhesion and motility. However, the N-glycosylation pattern of CD82 has not been descr...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Wang H,Zhang W,Zhao J,Zhang L,Liu M,Yan G,Yao J,Yu H,Yang P

    更新日期:2012-02-02 00:00:00

  • Quantitative proteomics analysis by iTRAQ revealed underlying changes in thermotolerance of Arthrospira platensis.

    abstract::Growth temperature is a critical factor that affects cultivation of Arthrospira platensis which is a type of cyanobacterium widely known as Spirulina that has significant commercial value. To investigate the molecular mechanism underlying the thermotolerance of Spirulina, differential protein expression profiling was ...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Chang R,Lv B,Li B

    更新日期:2017-08-08 00:00:00

  • Comparative bioinformatic and proteomic approaches to evaluate the outer membrane proteome of the fish pathogen Yersinia ruckeri.

    abstract::Yersinia ruckeri is the aetiological agent of enteric redmouth (ERM) disease and is responsible for significant economic losses in farmed salmonids. Enteric redmouth disease is associated primarily with rainbow trout (Oncorhynchus mykiss, Walbaum) but its incidence in Atlantic salmon (Salmo salar) is increasing. Outer...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Ormsby MJ,Grahame E,Burchmore R,Davies RL

    更新日期:2019-05-15 00:00:00

  • The benefits (and misfortunes) of SDS in top-down proteomics.

    abstract::Top-down proteomics (TDP) has great potential for high throughput proteoform characterization. With significant advances in mass spectrometry (MS) instrumentation permitting tandem MS of large intact proteins, a limitation to the widespread adoption of TDP still resides on front-end sample preparation protocols (e.g. ...

    journal_title:Journal of proteomics

    pub_type: 杂志文章,评审


    authors: Kachuk C,Doucette AA

    更新日期:2018-03-20 00:00:00

  • Urine metabolomic analysis in clear cell and papillary renal cell carcinoma: A pilot study.

    abstract::Renal cell carcinoma (RCC) is one of the most lethal type of tumors and is twice more frequent in men than in women. Initial symptoms are unspecific and belated thus increasing mortality. Moreover, current diagnostic and monitoring tools are harmful for the patient and unspecific in low-grade tumors. Therefore, novel ...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Oto J,Fernández-Pardo Á,Roca M,Plana E,Solmoirago MJ,Sánchez-González JV,Vera-Donoso CD,Martínez-Sarmiento M,España F,Navarro S,Medina P

    更新日期:2020-04-30 00:00:00

  • Proteome analysis of the fathead minnow (Pimephales promelas) reproductive testes.

    abstract::Proteomics is becoming more widely used as a tool in fish physiology and toxicology and can offer mechanistic insight into organism responses to environmental signals and stressors. Using a LTQ Orbitrap Velos MS/MS, we detected 1075 proteins in the reproductive testis of fathead minnow. Proteins localized to the testi...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Martyniuk CJ,Alvarez S

    更新日期:2013-02-21 00:00:00