Abstract:
:Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS) is a rapid methodology for identification of bacteria that is increasingly used in diagnostic laboratories. This work aimed at evaluating the potential of MALDI-TOF-MS for identification of the main serotypes of Flavobacterium psychrophilum isolated from salmonids, and its discrimination from closely related Flavobacterium spp. A mass spectra library was constructed by analysing 70 F. psychrophilum strains representing the serotypes O1, O2a, O2b and O3, including reference and clinical isolates. Peak mass lists were examined using the Mass-Up software for the detection of potential biomarkers, similarity and cluster analysis. Fourteen species-identifying biomarkers were detected in all the F. psychrophilum isolates tested, moreover, sets of serotype-identifying biomarkers ions were selected. F. psychrophilum-specific biomarkers were identified as ribosomal proteins by matching with protein databases. Furthermore, sequence variation corresponding to amino acid exchanges in several biomarker proteins were tentatively assigned. Closely related Flavobacterium species (F. flevense, F. succinicans, F. columnare, F. branchiophilum and F. johnsoniae) could be differentiated from F. psychrophilum by defining species identifying biomarkers and hierarchical cluster analysis. These results demonstrated that MALDI-TOF spectrometry represents a powerful tool for an accurate identification of the fish pathogen F. psychrophilum as well as for epidemiological studies. BIOLOGICAL SIGNIFICANCE:The results obtained in this study demonstrated that MALDI-TOF mass spectrometry represents a powerful tool that can be used by diagnostic laboratories for rapid identification of the fish pathogen Flavobacterium psychrophilum and its differentiation from other Flavobacterium-related species. Analysis of mass peak lists revealed the potential of the MALDI-TOF technique to identify epidemiologically important serotypes affecting salmonid fish. Furthermore, this study revealed the importance of the technique in the early and fast detection of bacterial pathogens, with the subsequent improvement in the health status and animal welfare and food safety in farmed fish.
journal_name
J Proteomicsjournal_title
Journal of proteomicsauthors
Fernández-Álvarez C,Torres-Corral Y,Santos Ydoi
10.1016/j.jprot.2017.09.007subject
Has Abstractpub_date
2018-01-06 00:00:00pages
59-69eissn
1874-3919issn
1876-7737pii
S1874-3919(17)30323-8journal_volume
170pub_type
杂志文章abstract:BACKGROUND:Oral leukoplakia is the most common potentially malignant disorder in the oral cavity and can precede carcinoma. This study aimed to identify possible oral leukoplakia salivary biomarkers. METHODS:Unstimulated saliva was collected from participants and protein concentration was determined. Proteins were the...
journal_title:Journal of proteomics
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journal_title:Journal of proteomics
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journal_title:Journal of proteomics
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doi:10.1016/j.jprot.2012.09.005
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abstract::With the rapid advance of MS-based proteomics one might think that 2D gel-based proteomics is dead. This is far from the truth. Current research has shown that there are still a number of places in the field of protein and molecular biology where 2D gels still play a leading role. The aim of this review is to highligh...
journal_title:Journal of proteomics
pub_type: 杂志文章,评审
doi:10.1016/j.jprot.2013.01.010
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doi:10.1016/j.jprot.2020.103797
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journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2012.09.020
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pub_type: 临床试验,杂志文章
doi:10.1016/j.jprot.2017.03.005
更新日期:2017-04-21 00:00:00
abstract::Mass spectrometry imaging (MSI) enables the direct analysis of molecules from the surface of a wide variety of samples, allowing the multiplex measurement of both abundance and distribution of small molecules, lipids, peptides and proteins. As the technology has been refined an increasing number of ionization methods ...
journal_title:Journal of proteomics
pub_type: 杂志文章,评审
doi:10.1016/j.jprot.2012.04.012
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journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2013.02.008
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journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2013.04.018
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abstract::Dystrobrevin is a dystrophin-related component of the dystrophin-associated protein complex (DAPC). Using alpha-dystrobrevin as indicator, we aimed to elucidate the interaction network of the DAPC with other proteins during apoptosis of promyelocytic HL-60 cells. The precise role(s) of DBs are not known, but we and ot...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2012.03.041
更新日期:2012-06-18 00:00:00
abstract::Gel electrophoresis-based and shotgun approaches are the most employed proteomic platforms in plant biology research, with the latter replacing the former in the last years. We have compared 2-DE-MALDI-TOF/TOF and GeLC-Orbitrap/MS analyses using the same protein extracts from Quercus ilex cotyledons at different devel...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2020.104087
更新日期:2021-02-20 00:00:00
abstract:UNLABELLED:Trichoderma reesei is the main industrial producer of lignocellulolytic enzymes, and the secretory behavior of this fungus strongly depends on the carbon sources. To gain insights into how the T. reesei adapts to various carbons and regulates enzyme production, the extra- and intracellular proteomes of T. re...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2013.06.014
更新日期:2013-08-26 00:00:00
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journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2012.10.001
更新日期:2013-04-09 00:00:00
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journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2015.07.021
更新日期:2016-02-16 00:00:00
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journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2014.05.023
更新日期:2014-08-28 00:00:00
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journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2015.08.011
更新日期:2016-02-16 00:00:00
abstract::Atrazine (ATZ), the second most commonly used herbicide in the United States, is an endocrine disrupting chemical linked to cancer and a common drinking water contaminant. This study further investigates ATZ-related developmental toxicity by testing the following hypotheses in zebrafish: the effects of embryonic ATZ e...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2018.07.006
更新日期:2018-08-30 00:00:00
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journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2012.11.023
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journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2017.07.011
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journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2017.06.020
更新日期:2017-08-23 00:00:00
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journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2013.04.010
更新日期:2014-01-31 00:00:00
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journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2020.103830
更新日期:2020-07-15 00:00:00
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journal_title:Journal of proteomics
pub_type: 杂志文章,评审
doi:10.1016/j.jprot.2011.10.008
更新日期:2011-12-10 00:00:00
abstract:UNLABELLED:The exoproteome of Leishmania infantum is composed of parasite derived proteins present in the extracellular environment. Although the exoproteome might have a significant role in the precocious steps of infection little is known concerning its composition. We developed an approach enabling the in vitro reco...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2013.03.012
更新日期:2013-06-12 00:00:00
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journal_title:Journal of proteomics
pub_type: 杂志文章,评审
doi:10.1016/j.jprot.2011.12.029
更新日期:2012-06-27 00:00:00
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journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2009.04.001
更新日期:2009-07-21 00:00:00
abstract:UNLABELLED:This study aimed at investigating the proteomic adaptation of Lactobacillus plantarum strains. Cultivation of L. plantarum strains under food-like conditions (wheat flour hydrolyzed, whey milk, tomato juice) affected some metabolic traits (e.g., consumption of carbohydrates and synthesis of organic acids) co...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2013.11.003
更新日期:2014-01-16 00:00:00
abstract::No effective screening method is available for oral squamous cell carcinoma (OSCC) that is recognized to influence by environmental factors as well as human papillomavirus (HPV) and Epstein-Barr virus (EBV). Therefore, we sought to identify salivary biomarkers for screening of OSCC with or without HPV and/or EBV infec...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2017.11.022
更新日期:2018-02-20 00:00:00