Abstract：
UNLABELLED:This study was conducted to define differences in Fusarium oxysporum f. sp. fragariae (Fof) isolates with different virulence efficiency to strawberry at the proteome level, in combination with their differences in mycelial growth, conidial production and germination. Comparative proteome analyses revealed substantial differences in mycelial proteomes between Fof isolates, where the 54 differentially accumulated protein spots were consistently over-accumulated or exclusively in the highly virulent isolate. These protein spots were identified through MALDI-TOF/TOF mass spectrometry analyses, and the identified proteins were mainly related to primary and protein metabolism, antioxidation, electron transport, cell cycle and transcription based on their putative functions. Proteins of great potential as Fof virulence factors were those involved in ubiquitin/proteasome-mediated protein degradation and reactive oxygen species detoxification; the hydrolysis-related protein haloacid dehalogenase superfamily hydrolase; 3,4-dihydroxy-2-butanone 4-phosphate synthase associated with riboflavin biosynthesis; and those exclusive to the highly virulent isolate. In addition, post-translational modifications may also make an important contribution to Fof virulence. BIOLOGICAL SIGNIFICANCE:F. oxysporum f. sp. fragariae (Fof), the causal agent of Fusarium wilt in strawberry, is a serious threat to commercial strawberry production worldwide. However, factors and mechanisms contributing to Fof virulence remained unknown. This study provides knowledge of the molecular basis for the differential expression of virulence in Fof, allowing new possibilities towards developing alternative and more effective strategies to manage Fusarium wilt.
journal_name
J Proteomicsjournal_title
Journal of proteomicsauthors
Fang X,Barbetti MJdoi
10.1016/j.jprot.2014.05.023subject
Has Abstractpub_date
2014-08-28 00:00:00pages
223-37eissn
1874-3919issn
1876-7737pii
S1874-3919(14)00293-0journal_volume
108pub_type
杂志文章abstract：:The somatic embryogenesis in conifers represents a suitable model of plant regeneration system facilitating studies of fundamental aspects of an early development as well as in vitro micropropagation. The aim of our study was to deeper understand the somatic embryogenesis in the conifer tree Pinus nigra Arn. Comparati...
journal_title：Journal of proteomics
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journal_title：Journal of proteomics
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journal_title：Journal of proteomics
pub_type： 杂志文章
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journal_title：Journal of proteomics
pub_type： 杂志文章
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journal_title：Journal of proteomics
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Journal of proteomics
pub_type： 杂志文章
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journal_title：Journal of proteomics
pub_type： 杂志文章
doi：10.1016/j.jprot.2011.02.033
更新日期：2011-05-16 00:00:00
abstract：:A combination between gel electrophoresis and smartphone technology is applied for quantifying proteins in a serum sample. Electrophoresis not only allows one to separate different proteins but also to build-up a calibration curve for those proteins. To expand its applicability, a smartphone allows one to capture a ge...
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pub_type： 杂志文章
doi：10.1016/j.jprot.2018.12.002
更新日期：2019-04-30 00:00:00
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journal_title：Journal of proteomics
pub_type： 杂志文章
doi：10.1016/j.jprot.2012.03.013
更新日期：2012-06-18 00:00:00
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journal_title：Journal of proteomics
pub_type： 杂志文章
doi：10.1016/j.jprot.2015.07.025
更新日期：2015-11-03 00:00:00
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journal_title：Journal of proteomics
pub_type： 杂志文章
doi：10.1016/j.jprot.2018.05.010
更新日期：2019-01-16 00:00:00
abstract：:Advances in mass spectrometry technology, high-throughput proteomics and genome annotations have resulted in significant increases in our molecular understanding of sperm composition. Using improved separation and detection methods and an updated genome annotation, a re-analysis of the Drosophila melanogaster sperm pr...
journal_title：Journal of proteomics
pub_type： 杂志文章
doi：10.1016/j.jprot.2010.09.002
更新日期：2010-10-10 00:00:00
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journal_title：Journal of proteomics
pub_type： 杂志文章
doi：10.1016/j.jprot.2013.06.031
更新日期：2013-10-08 00:00:00
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journal_title：Journal of proteomics
pub_type： 杂志文章,评审
doi：10.1016/j.jprot.2012.10.027
更新日期：2013-04-09 00:00:00
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journal_title：Journal of proteomics
pub_type： 杂志文章
doi：10.1016/j.jprot.2016.04.027
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journal_title：Journal of proteomics
pub_type： 杂志文章
doi：10.1016/j.jprot.2014.09.030
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journal_title：Journal of proteomics
pub_type： 杂志文章
doi：10.1016/j.jprot.2014.12.012
更新日期：2015-02-06 00:00:00
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journal_title：Journal of proteomics
pub_type： 杂志文章
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journal_title：Journal of proteomics
pub_type： 杂志文章
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pub_type： 杂志文章
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abstract：UNLABELLED:Mapping protein-protein interactions for chromatin-associated proteins remains challenging. Here we explore the use of BioID, a proximity biotinylation approach in which a mutated biotin ligase (BirA*) is fused to a bait of interest, allowing for the local activation of biotin and subsequent biotinylation of...
journal_title：Journal of proteomics
pub_type： 杂志文章
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更新日期：2015-04-06 00:00:00
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journal_title：Journal of proteomics
pub_type： 杂志文章
doi：10.1016/j.jprot.2017.06.013
更新日期：2017-08-08 00:00:00
abstract：:Trypanosoma cruzi proteins with molecular weight between 30 and 34 kDa have shown high reactivity in western blot assays with serum samples from chagasic individuals. However, in-depth analysis of the constituents of these protein fractions has not been performed. This is the first report of an immunoaffinity proteomi...
journal_title：Journal of proteomics
pub_type： 杂志文章
doi：10.1016/j.jprot.2012.11.001
更新日期：2013-03-27 00:00:00
abstract：:To seek a potential target for periodontal tissue regeneration, this study aimed to explore the role of Integrin alpha 5 (ITGA5) in human periodontal ligament stem cells (PDLSCs). Transwell assay, Cell Counting Kit 8 (CCK8) assay, cell cycle assay, alkaline phosphatase (ALP) activity, alizarin red staining, and wester...
journal_title：Journal of proteomics
pub_type： 杂志文章
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更新日期：2018-05-15 00:00:00
abstract：:Despite research progresses, the chance to accurately predict the risk for diabetic nephropathy (DN) is still poor. So far, the first evidence of DN is micro-albuminuria, which is detected only 10-20 years after the onset of diabetes. Our goal is to develop new predictive tools of nephropathy starting from urine, whic...
journal_title：Journal of proteomics
pub_type： 临床试验,杂志文章
doi：10.1016/j.jprot.2018.10.010
更新日期：2019-02-20 00:00:00
