Proteogenomic insight into the basis of the insecticide tolerance/resistance of the pollen beetle Brassicogethes (Meligethes) aeneus.


:The pollen beetle is a major pest of oilseed rape. Although various resistance mechanisms have been identified, such as kdr (mutation in the sodium channel) and metabolic resistance (CYP overexpression), other "hidden" factors also exist. Some studies have stressed the importance of epistasis as a genetic background. The combination of kdr and metabolic resistance appears to be unfavorable under field conditions in the absence of pesticide selection. The regulation of detoxification enzymes can play an important role, but we highlight different detoxification markers compared to those emphasized in other studies. We also stress the importance of studying the role of markers identified as pathogenesis-related protein 5-like (PR5; upregulated by insecticides) and highlight the role of RNA (DEAD-box) helicases (downregulated by insecticides). Thus, we suggest the importance of epigenetic drivers of resistance/tolerance to pesticides. The key results are similar to those of our previous study, in which deltamethrin treatment of the pollen beetle was also investigated by a proteogenomic approach. Indeed, the mechanism leading to resistance of the pollen beetle may be an innate mechanism that the pollen beetle can also employ in natural habitats, but under field conditions (pesticide exposure), this mechanism is used to survive in response to insecticides. SIGNIFICANCE: Pesticide resistance is a serious problem that hampers the successful production of crops. Understanding the mechanisms of insecticide resistance is highly important for successful pest control, especially when considering integrated pest management. Here, using a proteogenomic approach, we identified novel markers for understanding pollen beetle resistance to pesticides. In addition, future studies will reveal the role of these markers in the multiresistance of pollen beetle populations. We highlight that the proteins identified as PR5, which are known to occur in beetles and are similar to those in plants, may be responsible for tolerance to multiple stresses. In addition, our results indicate that the RNA helicases that exhibited changes in expression may be the epigenetic drivers of multiresistance. The nature of these changes remains an open question, and their relevance in different situations (responses to different stresses) in natural habitats in the absence of pesticides can be proposed.


J Proteomics


Journal of proteomics


Kocourek F,Stara J,Sopko B,Talacko P,Harant K,Hovorka T,Erban T




Has Abstract


2021-02-20 00:00:00












  • Reproducibility of combinatorial peptide ligand libraries for proteome capture evaluated by selected reaction monitoring.

    abstract:UNLABELLED:Systems biology studies require the capability to quantify with high precision proteins spanning a broad range of abundances across multiple samples. However, the broad range of protein expression in cells often precludes the detection of low-abundance proteins. Different sample processing techniques can be ...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Di Girolamo F,Righetti PG,Soste M,Feng Y,Picotti P

    更新日期:2013-08-26 00:00:00

  • Verification of a biomarker discovery approach for detection of Down syndrome in amniotic fluid via multiplex selected reaction monitoring (SRM) assay.

    abstract::Prenatal screening test for Down syndrome (DS) can be improved by discovery of novel biomarkers. A multiplex selected reaction monitoring (SRM) assay was developed to test previously identified thirteen candidate proteins in amniotic fluid (AF). One unique peptide was selected for each protein based on discovery data,...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Cho CK,Drabovich AP,Batruch I,Diamandis EP

    更新日期:2011-09-06 00:00:00

  • Hepatic proteomic analysis of selenoprotein F knockout mice by iTRAQ: An implication for the roles of selenoprotein F in metabolism and diseases.

    abstract::Selenoprotein F (Selenof) is an endoplasmic reticulum (ER)-resident protein. It may be functionally linked to glycoprotein folding in the ER but the detail function is not fully understood. To study the function of Selenof, we used CRISPR/Cas9 to generate Selenof knockout mice and performed proteomic analysis of hepat...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Zheng X,Ren B,Wang H,Huang R,Zhou J,Liu H,Tian J,Huang K

    更新日期:2020-03-20 00:00:00

  • A comparative study of the Se/S substitution in methionine and cysteine in Se-enriched yeast using an inductively coupled plasma mass spectrometry (ICP MS)-assisted proteomics approach.

    abstract:UNLABELLED:A proteomics approach based on 2D gel electrophoresis followed by HPLC-electrospray Orbitrap MS/MS was developed to investigate the replacement and the degree of the Se/S substitution in methionine and cysteine in Se-rich yeast. Capillary HPLC-inductively coupled plasma mass spectrometry (ICP-MS), employed i...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Bierla K,Bianga J,Ouerdane L,Szpunar J,Yiannikouris A,Lobinski R

    更新日期:2013-07-11 00:00:00

  • Characterizations and proteome analysis of platelet-free plasma-derived microparticles in β-thalassemia/hemoglobin E patients.

    abstract::Aggregatability and oxidative damage of red blood cells (RBCs), platelet activation and increased amount of blood cells-derived microparticles (MPs) are thought to be the etiologies for the thrombotic risk in thalassemia, but with unclear mechanisms. Here we report cellular origins and increases in number, oxidative s...

    journal_title:Journal of proteomics

    pub_type: 临床试验,杂志文章


    authors: Chaichompoo P,Kumya P,Khowawisetsut L,Chiangjong W,Chaiyarit S,Pongsakul N,Sirithanaratanakul N,Fucharoen S,Thongboonkerd V,Pattanapanyasat K

    更新日期:2012-12-05 00:00:00

  • Comparative proteomic analysis of sex-biased proteins in ovary and testis at different stages of Spodoptera litura.

    abstract::Sex-biased protein is thought to be able to drive the phenotypic differences in males and females in insects. In this study, 1385 and 1727 proteins were identified as differentially accumulated proteins (DAPs) by comparing the protein abundances at pupae stage with those at adult stage in ovary and testis of S.litura,...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Sun R,Sun Z,Chen Y,Zhu F,Li Y,Zhong G,Yi X

    更新日期:2019-08-30 00:00:00

  • Defining the transcriptomic and proteomic profiles of rat ageing skeletal muscle by the use of a cDNA array, 2D- and Blue native-PAGE approach.

    abstract::We defined the transcriptomic and proteomic profiles of rat ageing skeletal muscle using a combined cDNA array, 2D- and Blue native-PAGE approach. This was allowed to obtain an overview of the interrelated events leading to the transcriptome/proteome/mitoproteome changes likely to underlie the structural/metabolic fea...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Lombardi A,Silvestri E,Cioffi F,Senese R,Lanni A,Goglia F,de Lange P,Moreno M

    更新日期:2009-05-02 00:00:00

  • Peroxiredoxin-2 and zinc-alpha-2-glycoprotein as potentially combined novel salivary biomarkers for early detection of oral squamous cell carcinoma using proteomic approaches.

    abstract::No effective screening method is available for oral squamous cell carcinoma (OSCC) that is recognized to influence by environmental factors as well as human papillomavirus (HPV) and Epstein-Barr virus (EBV). Therefore, we sought to identify salivary biomarkers for screening of OSCC with or without HPV and/or EBV infec...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Heawchaiyaphum C,Pientong C,Phusingha P,Vatanasapt P,Promthet S,Daduang J,Teeramatwanich W,Kongyingyoes B,Chuerduangphui J,Ekalaksananan T

    更新日期:2018-02-20 00:00:00

  • Structural characterisation of medically relevant protein assemblies by integrating mass spectrometry with computational modelling.

    abstract::Structural mass spectrometry with its various techniques is a powerful tool for the structural elucidation of medically relevant protein assemblies. It delivers information on the composition, stoichiometries, interactions and topologies of these assemblies. Most importantly it can deal with heterogeneous mixtures and...

    journal_title:Journal of proteomics

    pub_type: 杂志文章,评审


    authors: Politis A,Schmidt C

    更新日期:2018-03-20 00:00:00

  • N-Glycosylation pattern of recombinant human CD82 (KAI1), a tumor-associated membrane protein.

    abstract::The membrane glycoprotein CD82 (KAI1) has attracted increasing attention as a suppressor of cell migration, related tumor invasion, as well as metastasis. The glycosylation of CD82 has been shown to be involved in a correlative cell adhesion and motility. However, the N-glycosylation pattern of CD82 has not been descr...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Wang H,Zhang W,Zhao J,Zhang L,Liu M,Yan G,Yao J,Yu H,Yang P

    更新日期:2012-02-02 00:00:00

  • Shotgun proteomic analysis of the unicellular alga Ostreococcus tauri.

    abstract::Ostreococcus tauri is a unicellular green alga and amongst the smallest and simplest free-living eukaryotes. The O. tauri genome sequence was determined in 2006. Molecular, physiological and taxonomic data that has been generated since then highlight its potential as a simple model species for algae and plants. Howeve...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Le Bihan T,Martin SF,Chirnside ES,van Ooijen G,Barrios-Llerena ME,O'Neill JS,Shliaha PV,Kerr LE,Millar AJ

    更新日期:2011-09-06 00:00:00

  • Oral administration of Bifidobacterium longum CECT 7347 modulates jejunal proteome in an in vivo gliadin-induced enteropathy animal model.

    abstract::Celiac disease is an immune-mediated disorder triggered by gluten proteins of wheat (gliadins) and other cereals. Gliadin-mediated effects on weanling animals, sensitized or not with interferon (IFN)-γ, were investigated. Also, the influence of the co-administration of Bifidobacterium longum CECT 7347 was studied toge...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Olivares M,Laparra M,Sanz Y

    更新日期:2012-12-21 00:00:00

  • The Trypanosoma rangeli trypomastigote surfaceome reveals novel proteins and targets for specific diagnosis.

    abstract:UNLABELLED:Sympatric distribution and sharing of hosts and antigens by Trypanosoma rangeli and Trypanosoma cruzi, the etiological agent of Chagas' disease, often incur in misdiagnosis and improper epidemiological inferences. Many secreted and surface proteins (SP) have been described as important antigens shared by the...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Wagner G,Eiko Yamanaka L,Moura H,Denardin Lückemeyer D,Schlindwein AD,Hermes Stoco P,Bunselmeyer Ferreira H,Robert Barr J,Steindel M,Grisard EC

    更新日期:2013-04-26 00:00:00

  • Proteomic analysis of liver in diet-induced Hyperlipidemic mice under Fructus Rosa roxburghii action.

    abstract::Fructus Rosae Roxburghii (FRR) has been considered as edible and medicinal fruit possessing antiatherosclerotic effect, but the mechanism is still unclear. HLP is material basis for AS formation. Under FRR action, TC, TG, LDL, HDL and ASI in serum were regulated to control level. Differentially expressed proteins in l...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Song P,Shen X

    更新日期:2021-01-06 00:00:00

  • Comparative proteomic analysis reveals that caspase-1 and serine protease may be involved in silkworm resistance to Bombyx mori nuclear polyhedrosis virus.

    abstract::The silkworm Bombyx mori is of great economic value. The B. mori nuclear polyhedrosis virus (BmNPV) is one of the most common and severe pathogens for silkworm. Although certain immune mechanisms exist in silkworms, most silkworms are still susceptible to BmNPV infection. Interestingly, BmNPV infection resistance in s...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Qin L,Xia H,Shi H,Zhou Y,Chen L,Yao Q,Liu X,Feng F,Yuan Y,Chen K

    更新日期:2012-06-27 00:00:00

  • Comparative iTRAQ proteomics revealed proteins associated with spermatogenic arrest of cattleyak.

    abstract:UNLABELLED:Male infertility of cattleyak due to spermatogenic arrest greatly restricts their effective utilization in yak breeding. Although much work has been done to investigate the mechanisms of spermatogenic arrest, there is no information regarding the differences of protein composition between cattleyak and yak t...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Yu S,Cai X,Sun L,Zuo Z,Mipam T,Cao S,Shen L,Ren Z,Chen X,Yang F,Deng J,Ma X,Wang Y

    更新日期:2016-06-16 00:00:00

  • Intrinsic disorder and multiple phosphorylations constrain the evolution of the flightin N-terminal region.

    abstract::Flightin is a myosin binding phosphoprotein that originated in the ancestor to Pancrustacea ~500 MYA. In Drosophila melanogaster, flightin is essential for length determination and flexural rigidity of thick filaments. Here, we show that among 12 Drosophila species, the N-terminal region is characterized by low sequen...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Lemas D,Lekkas P,Ballif BA,Vigoreaux JO

    更新日期:2016-03-01 00:00:00

  • Gender proteomics II. Which proteins in sexual organs.

    abstract::In continuity with the review dealing with differences by gender in non-sexual organs [1], this review collects data on the proteomes of the sexual organs as involved in human reproduction, under both physiological and pathological conditions. It also collects data on the tissue structures and biological fluids typica...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Gianazza E,Miller I,Guerrini U,Palazzolo L,Parravicini C,Eberini I

    更新日期:2018-04-30 00:00:00

  • Schistosome infections induce significant changes in the host biliary proteome.

    abstract:UNLABELLED:Schistosomiasis is a disease caused by blood trematodes affecting man and animals that represents an important human health and veterinary problem. Main damages caused by this infection are a consequence of the host inflammatory reaction against the parasite eggs trapped inside the liver. Despite that the he...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: de la Torre-Escudero E,Pérez-Sánchez R,Manzano-Román R,Oleaga A

    更新日期:2015-01-30 00:00:00

  • Proteomics in chromatin biology and epigenetics: Elucidation of post-translational modifications of histone proteins by mass spectrometry.

    abstract::Histone proteins contribute to the maintenance and regulation of the dynamic chromatin structure, to gene activation, DNA repair and many other processes in the cell nucleus. Site-specific reversible and irreversible post-translational modifications of histone proteins mediate biological functions, including recruitme...

    journal_title:Journal of proteomics

    pub_type: 杂志文章,评审


    authors: Sidoli S,Cheng L,Jensen ON

    更新日期:2012-06-27 00:00:00

  • Profiling of local disease-sparing responses to bovine respiratory syncytial virus in intranasally vaccinated and challenged calves.

    abstract::Bovine and human respiratory syncytial viruses (BRSV, HRSV) are primary causes of pneumonia in calves and children respectively, with vaccination offering protection via antibody and cellular immune responses. However, with no vaccines currently licensed for human use, evaluation of local responses to BRSV vaccination...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Gray D,Ellis JA,Gow SP,Lacoste SR,Allan GM,Mooney MH

    更新日期:2019-07-30 00:00:00

  • Proteomic characterization of the mucosal pellicle formed in vitro on a cellular model of oral epithelium.

    abstract::The oral mucosal pellicle is a thin lubricating layer generated by the binding of saliva proteins on epithelial oral cells. The protein composition of this biological structure has been to date studied by targeted analyses of specific salivary proteins. In order to perform a more exhaustive proteome characterization o...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Cabiddu G,Maes P,Hyvrier F,Olianas A,Manconi B,Brignot H,Canon F,Cabras T,Morzel M

    更新日期:2020-06-30 00:00:00

  • Proteomic changes in the heart of diet-induced pre-diabetic mice.

    abstract::The development of type 2 diabetes (T2D) is strongly associated with obesity. In humans, T2D increases the risk for end organ complications. Among these, heart disease has been ranked as the leading cause of death. We used a proteomic methodology to test the hypothesis that a pre-diabetic state generated by high-fat d...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Cruz-Topete D,List EO,Okada S,Kelder B,Kopchick JJ

    更新日期:2011-05-01 00:00:00

  • Proteome and phosphoproteome analysis of commensally induced dendritic cell maturation states.

    abstract::Dendritic cells (DCs) can shape the immune system towards an inflammatory or tolerant state depending on the bacterial antigens and the environment they encounter. In this study we provide a proteomic catalogue of differentially expressed proteins between distinct DC maturation states, brought about by bacteria that d...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Korkmaz AG,Popov T,Peisl L,Codrea MC,Nahnsen S,Steimle A,Velic A,Macek B,von Bergen M,Bernhardt J,Frick JS

    更新日期:2018-05-30 00:00:00

  • Peptidomic comparison and characterization of the major components of the venom of the giant ant Dinoponera quadriceps collected in four different areas of Brazil.

    abstract:UNLABELLED:Despite the noxious effects inflicted by Dinoponera ant's envenomation, the information about the biological properties and composition of their venom is still very limited. Ants from the genus Dinoponera are believed to be the world's largest living ants with a body length of 3cm. Their occurrence is restri...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Cologna CT,Cardoso Jdos S,Jourdan E,Degueldre M,Upert G,Gilles N,Uetanabaro AP,Costa Neto EM,Thonart P,de Pauw E,Quinton L

    更新日期:2013-12-06 00:00:00

  • Quantitative proteomic analysis reveals protein expression changes in the murine neuronal secretome during apoptosis.

    abstract::Neurodegenerative diseases often lack early and specific diagnostic and prognostic biomarkers. Many studies are focusing on the cerebrospinal fluid (CSF) proteome to identify relevant biomarkers and therapeutic targets for these disorders. An alternative approach consists in comparing proteins secreted by healthy neur...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Thouvenot E,Urbach S,Vigy O,Séveno M,Galéotti N,Nguyen G,Bockaert J,Marin P

    更新日期:2012-12-21 00:00:00

  • Molecular mass sorting of proteome using hollow fiber flow field-flow fractionation for proteomics.

    abstract::Hollow fiber flow field-flow fractionation (HF FlFFF) has been demonstrated as a tool for pre-fractionating proteomes by differences in molecular mass (Mr), where the resulting protein fractions are subsequently digested and analyzed by shotgun proteomics using two-dimensional liquid chromatography-electrospray ioniza...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Kim KH,Kang D,Koo HM,Moon MH

    更新日期:2008-04-30 00:00:00

  • Differential proteomics analysis to identify proteins and pathways associated with male sterility of soybean using iTRAQ-based strategy.

    abstract:UNLABELLED:To further elucidate the molecular mechanism of cytoplasmic male sterility (CMS) in soybean, a differential proteomic analysis was completed between the CMS line NJCMS1A and its maintainer NJCMS1B using iTRAQ-based strategy. As a result, 180 differential abundance proteins (DAPs) were identified, of which, 6...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Li J,Ding X,Han S,He T,Zhang H,Yang L,Yang S,Gai J

    更新日期:2016-04-14 00:00:00

  • Proteomic changes in Arthrobotrys oligospora conidia in response to benzaldehyde-induced fungistatic stress.

    abstract::Soil fungistasis limits the effect of fungal agents designed to control plant-parasitic nematodes. Benzaldehyde is a fungistatic factor produced by soil microorganisms that can suppress conidial germination, but the molecular mechanism of this suppression is unknown. In this study, three conidial proteomes of Arthrobo...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Liu T,Zou LJ,Tian DW,Can QY,Zhu ML,Mo MH,Zhang KQ

    更新日期:2019-02-10 00:00:00

  • Proteomic analysis of the effect of plant-derived smoke on soybean during recovery from flooding stress.

    abstract::Flooding negatively affects the growth of soybean, whereas the plant-derived smoke enhances seedling growth of crops. To clarify the mechanism underlying the recovery from flooding stress, proteomic analysis was performed based on morphological results. Growth of soybean seedlings was inhibited under flooding stress, ...

    journal_title:Journal of proteomics

    pub_type: 杂志文章


    authors: Li X,Rehman SU,Yamaguchi H,Hitachi K,Tsuchida K,Yamaguchi T,Sunohara Y,Matsumoto H,Komatsu S

    更新日期:2018-06-15 00:00:00