Abstract:
UNLABELLED:We attempted to identify biomarkers that would predict responsiveness of osteosarcoma (OS) to induction chemotherapy. Tumor tissues obtained by open biopsy before induction chemotherapy were investigated. On the basis of histological observations at the time of surgery and the Huvos grading system, 7 patients were classified as good responders and the other 6 as poor responders. Protein expression profiling was performed by two-dimensional difference gel electrophoresis. Among 3494 protein spots observed, the intensity of 33 spots was found to differ significantly between the two patient groups. The proteins for these 33 protein spots were identified by mass spectrometry. The higher expression of peroxiredoxin 2 (PRDX2) in poor responders was confirmed by Western blotting. Gene silencing assay demonstrated that reduced expression of PRDX2 was associated with increased sensitivity of OS cells to chemotherapeutic drugs such as methotrexate, doxorubicin and cisplatin. Moreover, siRNA-induced silencing of PRDX2 resulted in a decrease of cell proliferation, invasion and migration. These findings indicated that PRDX2 would be a candidate biomarker of response to induction chemotherapy. Measurement of PRDX2 in open biopsy samples before treatment may contribute to risk stratification therapy for OS. BIOLOGICAL SIGNIFICANCE:The response of osteosarcoma patients to induction chemotherapy is critical because the prognosis of responders is quite favorable, whereas that of non-responders is poor. Although there are many therapeutic options for osteosarcoma, no parameter for predicting the response to induction chemotherapy has been available. We conducted a proteomics study aimed at developing a biomarker that would predict the response of osteosarcoma to induction chemotherapy. Using open biopsy samples obtained before chemotherapy, we conducted 2D-DIGE with our originally devised large-format electrophoresis apparatus and identified peroxiredoxin 2 (PRDX2) as a novel predictive biomarker. The diagnostic performance of PRDX2 was confirmed by ROC analysis, and its functional properties were investigated in a series of in vitro functional assays. Our findings indicate the possible application of PRDX2 as a predictive biomarker in patients with osteosarcoma.
journal_name
J Proteomicsjournal_title
Journal of proteomicsauthors
Kubota D,Mukaihara K,Yoshida A,Tsuda H,Kawai A,Kondo Tdoi
10.1016/j.jprot.2013.07.022subject
Has Abstractpub_date
2013-10-08 00:00:00pages
393-404eissn
1874-3919issn
1876-7737pii
S1874-3919(13)00414-4journal_volume
91pub_type
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journal_title:Journal of proteomics
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abstract:UNLABELLED:Male infertility of cattleyak due to spermatogenic arrest greatly restricts their effective utilization in yak breeding. Although much work has been done to investigate the mechanisms of spermatogenic arrest, there is no information regarding the differences of protein composition between cattleyak and yak t...
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abstract:UNLABELLED:Milk and dairy products are central elements in the human diet. It is estimated that 108kg of milk per year are consumed per person worldwide. Therefore, dairy production represents a relevant fraction of the economies of many countries, being cattle, sheep, goat, water buffalo, and other ruminants the main ...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2013.09.012
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pub_type: 杂志文章
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journal_title:Journal of proteomics
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journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2010.08.011
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journal_title:Journal of proteomics
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doi:10.1016/j.jprot.2014.06.020
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journal_title:Journal of proteomics
pub_type: 杂志文章
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pub_type: 历史文章,杂志文章
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doi:10.1016/j.jprot.2015.04.010
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journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2011.04.001
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journal_title:Journal of proteomics
pub_type: 杂志文章,评审
doi:10.1016/j.jprot.2009.07.004
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journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2014.07.029
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doi:10.1016/j.jprot.2012.03.013
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pub_type: 临床试验,杂志文章
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pub_type: 杂志文章
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journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2011.06.021
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journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2018.04.031
更新日期:2018-06-15 00:00:00
abstract:UNLABELLED:Escherichia coli is a commensal microorganism of the gastrointestinal tract of animals and humans and it is an excellent model organism for the study of antibiotic resistance mechanisms. The resistance transmission and other characteristics of bacteria are based on different types of gene transfer occurring ...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2016.03.042
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journal_title:Journal of proteomics
pub_type: 杂志文章
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journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2018.06.010
更新日期:2018-07-30 00:00:00
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journal_title:Journal of proteomics
pub_type: 临床试验,杂志文章
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