Abstract:
UNLABELLED:Mitral regurgitation (MR) is a common valvular lesion frequently caused by mitral valve prolapse (MVP). Surgical intervention in MVP patients with significant MR is predicated on symptoms and measures of left ventricular dysfunction. Because these indicators may be subjective or imprecise, serological biomarkers of disease could be a valuable adjunct to standard evaluation. This study aimed to identify such biomarkers by a proteomics approach. Two pooled plasma samples from 24 MVP subjects with MR (MVP/MR) and 24 non-MVP individuals were treated with the combinatorial peptide ligand library (CPLL) beads prior to iTRAQ labeling and ESI-MS/MS. Lower levels of haptoglobin, platelet basic protein (PBP), and complement component C4b were observed in the MVP/MR as compared to the control sample. These findings were verified by ELISA testing of each of the 24 paired samples, and another 42 matched cases and controls. The AUC values, sensitivities and specificities for (i) haptoglobin, (ii) PBP, (iii) C4b, and (iv) all 3 proteins in combination were (i) 0.813, 76%, 74%; (ii) 0.721, 56%, 77%; (iii) 0.689, 83%, 49%; and (iv) 0.840, 89%, 67%, respectively. In conclusion, haptoglobin, PBP, and C4b are down-regulated in MVP/MR. Their value as serological biomarkers of valvular pathology should be further explored. BIOLOGICAL SIGNIFICANCE:We report the first study that performed comparative proteomics of clinical human plasma samples to identify novel diagnostic biomarkers for mitral valve prolapse (MVP) patients with moderate to severe mitral regurgitation (MR). MR is a common valvular lesion that can be complicated by heart failure, sudden death and atrial fibrillation, yet many patients with severe MR are asymptomatic. Our results revealed reduced levels of haptoglobin, platelet basic protein (PBP), and complement component C4b in the MVP/MR patients as compared to the matched control cases. The plasma proteomics findings were subsequently confirmed by ELISA. Each of these candidate biomarkers has a putative role in the pathophysiology of MVP/MR, further supporting their roles in detection and possibly surveillance and prognostication of this disease.
journal_name
J Proteomicsjournal_title
Journal of proteomicsauthors
Tan HT,Ling LH,Dolor-Torres MC,Yip JW,Richards AM,Chung MCdoi
10.1016/j.jprot.2013.10.009subject
Has Abstractpub_date
2013-12-06 00:00:00pages
337-45eissn
1874-3919issn
1876-7737pii
S1874-3919(13)00518-6journal_volume
94pub_type
临床试验,杂志文章abstract:INTRODUCTION:Juvenile idiopathic arthritis (JIA) comprises a poorly understood group of chronic autoimmune diseases with variable clinical outcomes. We investigated whether the synovial fluid (SF) proteome could distinguish a subset of patients in whom disease extends to affect a large number of joints. METHODS:SF sam...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2012.06.024
更新日期:2012-09-18 00:00:00
abstract::Human populations are constantly plagued by hematophagous insects' bites, in particular the triatomine insects that are vectors of the Trypanosoma cruzi agent in Chagas disease. The pharmacologically-active molecules present in the salivary glands of hematophagous insects are injected into the human skin to initiate a...
journal_title:Journal of proteomics
pub_type: 杂志文章,评审
doi:10.1016/j.jprot.2012.05.001
更新日期:2012-07-16 00:00:00
abstract::Wilson's disease is a rare inherited disorder of copper metabolism, manifesting hepatic, neurological and psychiatric symptoms. Early diagnosis is often unfeasible and a unique diagnostic test is currently inapplicable. We performed the qualitative/quantitative characterization of the salivary proteome/peptidome of 32...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2015.07.033
更新日期:2015-10-14 00:00:00
abstract:UNLABELLED:The proteome of the venom of Naja haje legionis, the only medically important elapid species in Morocco, has been elucidated by using a combination of proteomic techniques that includes size exclusion chromatography, reverse-phase HPLC, Tricine/SDS-Page, tryptic digestion, Q-TOF tandem mass spectrometry and ...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2013.11.012
更新日期:2014-01-16 00:00:00
abstract::High content omic techniques in combination with stable human in vitro cell culture systems have the potential to improve on current pre-clinical safety regimes by providing detailed mechanistic information of altered cellular processes. Here we investigated the added benefit of integrating transcriptomics, proteomics...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2012.11.022
更新日期:2013-02-21 00:00:00
abstract::An automated image analysis system was used for protein quantification of 1862 human proteins in 47 cancer cell lines and 12 clinical cell samples using cell microarrays and immunohistochemistry. The analysis suggests that most proteins are expressed in a cell size dependent manner, and that normalization is required ...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2008.06.014
更新日期:2008-10-07 00:00:00
abstract::Fatty acid desaturate 2 (Fads2) is associated with many chronic diseases. Nevertheless, comprehensive researches on its role have not been performed. We here conducted an integrated analysis of long-chain polyunsaturated fatty acid (LC-PUFA) metabolism of fads2-deletion zebrafish (fads2-/-) by transcriptomics, proteom...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2020.103967
更新日期:2020-10-30 00:00:00
abstract::Sheep have a variable ability to resist gastrointestinal nematode infection, but the key factors mediating this response are poorly defined. Here we report the first large-scale application of quantitative proteomic technologies to define proteins that are differentially abundant between sheep selectively bred to have...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2012.01.016
更新日期:2012-04-03 00:00:00
abstract::Populations of small eukaryotic RNAs, in addition to relatively well recognized molecules such as miRNAs or siRNAs, also contain fragments derived from all classes of constitutively expressed non-coding RNAs. It has been recently demonstrated that the formation and accumulation of RNA fragments (RFs) is cell-/tissue-s...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2018.10.007
更新日期:2019-02-20 00:00:00
abstract::Histone proteins contribute to the maintenance and regulation of the dynamic chromatin structure, to gene activation, DNA repair and many other processes in the cell nucleus. Site-specific reversible and irreversible post-translational modifications of histone proteins mediate biological functions, including recruitme...
journal_title:Journal of proteomics
pub_type: 杂志文章,评审
doi:10.1016/j.jprot.2011.12.029
更新日期:2012-06-27 00:00:00
abstract::Flooding negatively affects the growth of soybean, whereas the plant-derived smoke enhances seedling growth of crops. To clarify the mechanism underlying the recovery from flooding stress, proteomic analysis was performed based on morphological results. Growth of soybean seedlings was inhibited under flooding stress, ...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2018.04.031
更新日期:2018-06-15 00:00:00
abstract::The process of protein phosphorylation in cells is well studied in the context of a wide range of biologic functions such as signalling, cell cycle, cell growth and differentiation, and others. In contrast, little progress has been made in the investigation of protein phosphorylation specifically in blood. Here, we fo...
journal_title:Journal of proteomics
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1016/j.jprot.2012.02.015
更新日期:2012-12-05 00:00:00
abstract::A phaC knockout mutant from Bacillus thuringiensis (Bt) strain BMB171, named BMB171/PHB(-1), was constructed. A physiological and metabolic investigation and a proteomic analysis were conducted for both ΔphaC mutant and its parent strain. Grown in peptone medium with 5 gram glucose per liter as sole carbon source, BMB...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2012.06.002
更新日期:2012-09-18 00:00:00
abstract::Molecular initiating events and downstream transcriptional/proteomic responses provide valuable information for adverse outcome pathways, which can be used predict the effects of chemicals on physiological systems. There has been a paucity of research that addresses sex-specific expression profiling in toxicology and ...
journal_title:Journal of proteomics
pub_type: 杂志文章,评审
doi:10.1016/j.jprot.2017.09.010
更新日期:2018-02-10 00:00:00
abstract::Plant-derived smoke plays a key role in seed germination and plant growth. To investigate the effect of plant-derived smoke on chickpea, a gel-free/label-free proteomic technique was used. Germination percentage, root/shoot length, and fresh biomass were increased in chickpea treated with 2000 ppm plant-derived smoke ...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2018.01.011
更新日期:2018-03-30 00:00:00
abstract::Physical activity improves overall health and counteracts metabolic pathologies. Adipose tissue and bone are important key targets of exercise; the prevalence of diseases associated with suboptimal physical activity levels has increased in recent times as a result of lifestyle changes. Mesenchymal stem cells (MSCs) di...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2017.09.004
更新日期:2018-01-06 00:00:00
abstract:UNLABELLED:Increasing atmospheric carbon dioxide results in ocean acidification and warming, significantly impacting marine invertebrate larvae development. We investigated how ocean acidification in combination with warming affected D-veliger larvae of the Pacific oyster Crassostrea gigas. Larvae were reared for 40h u...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2015.12.001
更新日期:2016-03-01 00:00:00
abstract::Ostreococcus tauri is a unicellular green alga and amongst the smallest and simplest free-living eukaryotes. The O. tauri genome sequence was determined in 2006. Molecular, physiological and taxonomic data that has been generated since then highlight its potential as a simple model species for algae and plants. Howeve...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2011.05.028
更新日期:2011-09-06 00:00:00
abstract::A large series of protein pathway components have been shown to be dysregulated in Down syndrome (DS) brain. No information about pathomechanisms linked to the trisomic state can be obtained from adult DS brain, however, as neurodegeneration occurs from the fourth decade. The aim of the study was to search for protein...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2011.01.009
更新日期:2011-04-01 00:00:00
abstract:UNLABELLED:A proteomics approach based on 2D gel electrophoresis followed by HPLC-electrospray Orbitrap MS/MS was developed to investigate the replacement and the degree of the Se/S substitution in methionine and cysteine in Se-rich yeast. Capillary HPLC-inductively coupled plasma mass spectrometry (ICP-MS), employed i...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2013.05.010
更新日期:2013-07-11 00:00:00
abstract:UNLABELLED:The genus Mentha has been widely used in food, flavor, culinary, cosmetic and pharmaceutical industries. Substantial damage to this crop happened regularly due to environmental stresses like metal toxicity and pathogen attack. Here, an approach has been taken to raise transgenic mint over-expressing γ-glutam...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2013.01.020
更新日期:2013-11-20 00:00:00
abstract::Despite research progresses, the chance to accurately predict the risk for diabetic nephropathy (DN) is still poor. So far, the first evidence of DN is micro-albuminuria, which is detected only 10-20 years after the onset of diabetes. Our goal is to develop new predictive tools of nephropathy starting from urine, whic...
journal_title:Journal of proteomics
pub_type: 临床试验,杂志文章
doi:10.1016/j.jprot.2018.10.010
更新日期:2019-02-20 00:00:00
abstract::Hollow fiber flow field-flow fractionation (HF FlFFF) has been demonstrated as a tool for pre-fractionating proteomes by differences in molecular mass (Mr), where the resulting protein fractions are subsequently digested and analyzed by shotgun proteomics using two-dimensional liquid chromatography-electrospray ioniza...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2008.02.001
更新日期:2008-04-30 00:00:00
abstract::Gel electrophoresis-based and shotgun approaches are the most employed proteomic platforms in plant biology research, with the latter replacing the former in the last years. We have compared 2-DE-MALDI-TOF/TOF and GeLC-Orbitrap/MS analyses using the same protein extracts from Quercus ilex cotyledons at different devel...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2020.104087
更新日期:2021-02-20 00:00:00
abstract::Hyperaccumulators are distinguished from non-hyperaccumulators on the basis of their capacity to extract heavy metal ions from the soil, their more efficient root-to-shoot translocation of these ions and their greater ability to detoxify and sequester heavy metals in the shoot. The understanding of the mechanisms unde...
journal_title:Journal of proteomics
pub_type: 杂志文章,评审
doi:10.1016/j.jprot.2012.12.006
更新日期:2013-02-21 00:00:00
abstract:UNLABELLED:Despite the noxious effects inflicted by Dinoponera ant's envenomation, the information about the biological properties and composition of their venom is still very limited. Ants from the genus Dinoponera are believed to be the world's largest living ants with a body length of 3cm. Their occurrence is restri...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2013.10.017
更新日期:2013-12-06 00:00:00
abstract::In recent years, affinity-based technologies have become important tools for serum profiling to uncover protein expression patterns linked to disease state or therapeutic effects. In this study, we describe a path towards the production of an antibody microarray to allow protein profiling of biotinylated human serum s...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2009.09.009
更新日期:2009-12-01 00:00:00
abstract::Lignocellulosic plant biomass is the most abundant carbon source in the planet, which makes it a potential substrate for biorefinery. It consists of polysaccharides and other molecules with applications in pharmaceutical, food and feed, cosmetics, paper and textile industries. The exploitation of these resources requi...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2018.02.012
更新日期:2018-04-15 00:00:00
abstract::Donkey's milk is today categorized among the best mother's milk substitute for allergic newborns, due to its much reduced or absent allergenicity, coupled to excellent palatability and nutritional value. However, up to the present, only a handful of proteins had been characterized, just about the standard eight to ten...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2011.05.036
更新日期:2011-09-06 00:00:00
abstract:UNLABELLED:This study constitutes the first attempt to understand at the proteomic level the fungal response to ionic liquid stress. Ascomycota are able to grow in media supplemented with high concentrations of an ionic liquid, which, in turn, lead to major alterations in the fungal metabolic footprint. Herein, we anal...
journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2013.09.015
更新日期:2013-12-06 00:00:00