当前位置： SCI文献检索 > Journal of Proteomics期刊下所有文献 > Proteomic analysis of Bacillus thuringiensis ΔphaC mutant BMB171/PHB(-1) reveals that the PHB synthetic pathway warrants normal carbon metabolism.

Proteomic analysis of Bacillus thuringiensis ΔphaC mutant BMB171/PHB(-1) reveals that the PHB synthetic pathway warrants normal carbon metabolism.

Abstract：

:A phaC knockout mutant from Bacillus thuringiensis (Bt) strain BMB171, named BMB171/PHB(-1), was constructed. A physiological and metabolic investigation and a proteomic analysis were conducted for both ΔphaC mutant and its parent strain. Grown in peptone medium with 5 gram glucose per liter as sole carbon source, BMB171/PHB(-1) produced various organic acids. Here the excreted pyruvate, citrate, lactate, acetate and glutamate were quantitatively analyzed. Deletion of phaC gene from the BMB171 strain resulted in 1) growth delay; 2) higher consumption of dioxigen but lower cell yield; 3) stagnation of pH movement; 4) overproduction of organic acids; 5) rapid descent of cell density in the stationary phase; and 6) a sporulation-deficient phenotype. Our proteomic study with qPCR reconfirmation reveals that the absence of PhaC led to a metabolic turmoil which showed repressed glycolysis, and over-expressed TCA cycle, various futile pathways and amino acid synthesis during vegetative growth. It is thus thought that B. thuringiensis BMB171 effectively regulated its carbon metabolism upon the presence of the functional PHB synthetic pathway. The presence of this pathway warrants a PHB-producing bacterium better surviving under different environmental conditions.

journal_name

J Proteomics

journal_title

Journal of proteomics

authors

Chen D,Xu D,Li M,He J,Gong Y,Wu D,Sun M,Yu Z

doi

10.1016/j.jprot.2012.06.002

subject

Has Abstract

pub_date

2012-09-18 00:00:00

pages

5176-88

issue

17

eissn

1874-3919

issn

1876-7737

pii

S1874-3919(12)00426-5

journal_volume

75

pub_type

杂志文章

相关文献

Journal of Proteomics文献大全
  • Construction of nanometer cisplatin core-ferritin (NCC-F) and proteomic analysis of gastric cancer cell apoptosis induced with cisplatin released from the NCC-F.

    abstract：:Both transmission electron microscopy (TEM) and fluorescence spectrometry were used to reveal the characteristics of both subunit disassociation and recombination in apo-pig pancreas ferritin (apoPPF) in an alkaline medium ranging reversibly from pH 7.0 to 13.0. The experimental results indicated that apoPPF could be ...

    journal_title：Journal of proteomics

    pub_type： 杂志文章

    doi：10.1016/j.jprot.2012.03.013

    authors： Ji XT,Huang L,Huang HQ

    更新日期：2012-06-18 00:00:00

  • An integrated immunoproteomics and bioinformatics approach for the analysis of Schistosoma japonicum tegument proteins.

    abstract：UNLABELLED:Schistosomiasis remains one of the major neglected tropical diseases (NTDs) causing morbidity of humans residing in the tropical countries. Much effort has been devoted to the development of vaccines, since it is recognized that vaccines can be served as an important supplementary component alongside chemoth...

    journal_title：Journal of proteomics

    pub_type： 杂志文章

    doi：10.1016/j.jprot.2014.01.010

    authors： Chen JH,Zhang T,Ju C,Xu B,Lu Y,Mo XJ,Chen SB,Fan YT,Hu W,Zhou XN

    更新日期：2014-02-26 00:00:00

  • Proteomics study of open biopsy samples identifies peroxiredoxin 2 as a predictive biomarker of response to induction chemotherapy in osteosarcoma.

    abstract：UNLABELLED:We attempted to identify biomarkers that would predict responsiveness of osteosarcoma (OS) to induction chemotherapy. Tumor tissues obtained by open biopsy before induction chemotherapy were investigated. On the basis of histological observations at the time of surgery and the Huvos grading system, 7 patient...

    journal_title：Journal of proteomics

    pub_type： 杂志文章

    doi：10.1016/j.jprot.2013.07.022

    authors： Kubota D,Mukaihara K,Yoshida A,Tsuda H,Kawai A,Kondo T

    更新日期：2013-10-08 00:00:00

  • Revealing the functional structure of a new PLA2 K49 from Bothriopsis taeniata snake venom employing automatic "de novo" sequencing using CID/HCD/ETD MS/MS analyses.

    abstract：:Snake venoms are composed of approximately 90% of proteins with several pharmacological activities having high potential in research as biological tools. One of the most abundant compounds is phospholipases A2 (PLA2), which are the most studied venom protein due to their wide pharmacological activity. Using a combinat...

    journal_title：Journal of proteomics

    pub_type： 杂志文章

    doi：10.1016/j.jprot.2015.10.020

    authors： Carregari VC,Dai J,Verano-Braga T,Rocha T,Ponce-Soto LA,Marangoni S,Roepstorff P

    更新日期：2016-01-10 00:00:00

  • Surface N-glycoproteome patterns reveal key proteins of neuronal differentiation.

    abstract：UNLABELLED:Pluripotent stem cell-derived committed neural precursors are an important source of cells to treat neurodegenerative diseases including spinal cord injury. There remains an urgency to identify markers for monitoring of neural progenitor specificity, estimation of neural fate and follow-up correlation with t...

    journal_title：Journal of proteomics

    pub_type： 杂志文章

    doi：10.1016/j.jprot.2015.11.008

    authors： Tyleckova J,Valekova I,Zizkova M,Rakocyova M,Marsala S,Marsala M,Gadher SJ,Kovarova H

    更新日期：2016-01-30 00:00:00

  • Comparative proteomic analysis reveals that caspase-1 and serine protease may be involved in silkworm resistance to Bombyx mori nuclear polyhedrosis virus.

    abstract：:The silkworm Bombyx mori is of great economic value. The B. mori nuclear polyhedrosis virus (BmNPV) is one of the most common and severe pathogens for silkworm. Although certain immune mechanisms exist in silkworms, most silkworms are still susceptible to BmNPV infection. Interestingly, BmNPV infection resistance in s...

    journal_title：Journal of proteomics

    pub_type： 杂志文章

    doi：10.1016/j.jprot.2012.04.015

    authors： Qin L,Xia H,Shi H,Zhou Y,Chen L,Yao Q,Liu X,Feng F,Yuan Y,Chen K

    更新日期：2012-06-27 00:00:00

  • Reproducibility of combinatorial peptide ligand libraries for proteome capture evaluated by selected reaction monitoring.

    abstract：UNLABELLED:Systems biology studies require the capability to quantify with high precision proteins spanning a broad range of abundances across multiple samples. However, the broad range of protein expression in cells often precludes the detection of low-abundance proteins. Different sample processing techniques can be ...

    journal_title：Journal of proteomics

    pub_type： 杂志文章

    doi：10.1016/j.jprot.2013.05.037

    authors： Di Girolamo F,Righetti PG,Soste M,Feng Y,Picotti P

    更新日期：2013-08-26 00:00:00

  • Identification of novel interacting partners of the NEDD4 ubiquitin ligase in mouse testis.

    abstract：:Posttranslational modification by ubiquitination targets proteins for degradation, recycling, stabilization or altered trafficking, and as such can alter cellular signaling pathways. The substrate specificity of this multistep process is controlled by ubiquitin ligases, including those of the HECT domain-containing NE...

    journal_title：Journal of proteomics

    pub_type： 杂志文章

    doi：10.1016/j.jprot.2020.103830

    authors： Manning J,Windley SP,Sandow JJ,Shah SS,Western P,Wilhelm D,Kumar S

    更新日期：2020-07-15 00:00:00

  • S-Nitrosylation in plants: pattern and function.

    abstract：:During the last two decades nitric oxide (NO) has emerged as a new chemical messenger in plant biology, which is involved in many different physiological processes, such as plant defense, transpiration and gas exchange, seed germination, and root development. Protein S-nitrosylation, the post-translational modificatio...

    journal_title：Journal of proteomics

    pub_type： 杂志文章,评审

    doi：10.1016/j.jprot.2009.07.002

    authors： Lindermayr C,Durner J

    更新日期：2009-11-02 00:00:00

  • Proteome response of dental pulp cells to exogenous FGF8.

    abstract：:FGF8 specifies early tooth development by directing the migration of the early tooth founder cells to the site of tooth emergence. To date the effect of the FGF8 in adult dental pulp has not been studied. We have assessed the regenerative potential of FGF8 by evaluating changes in the proteome landscape of dental pulp...

    journal_title：Journal of proteomics

    pub_type： 杂志文章

    doi：10.1016/j.jprot.2018.05.004

    authors： Tsikandelova R,Mladenov P,Planchon S,Kalenderova S,Praskova M,Mihaylova Z,Stanimirov P,Mitev V,Renaut J,Ishkitiev N

    更新日期：2018-07-15 00:00:00

  • A proteomic analysis of p53-independent induction of apoptosis by bortezomib in 4T1 breast cancer cell line.

    abstract：UNLABELLED:The 26S proteasome is a proteolytic enzyme found in both cytoplasm and nucleus. In this study, we examined the differential expression of proteasome inhibitor bortezomib-induced proteins in p53-deficient 4T1 cells. It was found that GRP78 and TCEB2 were over-expressed in response to treatment with bortezomib...

    journal_title：Journal of proteomics

    pub_type： 杂志文章

    doi：10.1016/j.jprot.2014.09.010

    authors： Yerlikaya A,Okur E,Baykal AT,Acılan C,Boyacı I,Ulukaya E

    更新日期：2015-01-15 00:00:00

  • In situ cell surface proteomics reveals differentially expressed membrane proteins in retinal pigment epithelial cells during autoimmune uveitis.

    abstract：UNLABELLED:Retinal pigment epithelium (RPE) builds the outer blood-retinal barrier of the eye and plays an important role in pathogenesis of the sight threatening disease equine recurrent uveitis (ERU). ERU is a spontaneous autoimmune mediated inflammatory disease characterised by the breakdown of the outer blood-retin...

    journal_title：Journal of proteomics

    pub_type： 杂志文章

    doi：10.1016/j.jprot.2014.06.020

    authors： Uhl PB,Szober CM,Amann B,Alge-Priglinger C,Ueffing M,Hauck SM,Deeg CA

    更新日期：2014-09-23 00:00:00

  • Earwax: A neglected body secretion or a step ahead in clinical diagnosis? A pilot study.

    abstract：:This work combines the advantages of volatile metabolites profiling as a young growing research field with a non-invasive sampling technique using earwax "a neglected body secretion" for detection and monitoring of biomarkers for diabetes mellitus (types 1 and 2). Earwax samples were collected from 26 diabetic patient...

    journal_title：Journal of proteomics

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.jprot.2017.03.005

    authors： Shokry E,de Oliveira AE,Avelino MAG,de Deus MM,Filho NRA

    更新日期：2017-04-21 00:00:00

  • Impact of HDL oxidation by the myeloperoxidase system on sterol efflux by the ABCA1 pathway.

    abstract：:Protein oxidation by phagocytic white blood cells is implicated in tissue injury during inflammation. One important target might be high-density lipoprotein (HDL), which protects against atherosclerosis by removing excess cholesterol from artery wall macrophages. In the human artery wall, cholesterol-laden macrophages...

    journal_title：Journal of proteomics

    pub_type： 杂志文章

    doi：10.1016/j.jprot.2011.04.001

    authors： Shao B,Heinecke JW

    更新日期：2011-10-19 00:00:00

  • Development of liquid microjunction extraction strategy for improving protein identification from tissue sections.

    abstract：:MALDI Mass Spectrometry Imaging has shown important potential for molecular classification and pathology marker discovery. Protein markers identification is therefore of prime importance. Direct structural analysis from tissue sections has shown limitations for protein identification because of the high degree of comp...

    journal_title：Journal of proteomics

    pub_type： 杂志文章

    doi：10.1016/j.jprot.2012.11.025

    authors： Quanico J,Franck J,Dauly C,Strupat K,Dupuy J,Day R,Salzet M,Fournier I,Wisztorski M

    更新日期：2013-02-21 00:00:00

  • Usage of electrostatic eliminator reduces human keratin contamination significantly in gel-based proteomics analysis.

    abstract：:In the field of bottom-up proteomics, heavy contamination of human keratins could hinder the comprehensive protein identification, especially for the detection of low abundance proteins. In this study, we examined the keratin contamination in the four major experimental procedures in gel-based proteomic analysis inclu...

    journal_title：Journal of proteomics

    pub_type： 杂志文章

    doi：10.1016/j.jprot.2011.03.001

    authors： Xu B,Zhang Y,Zhao Z,Yoshida Y,Magdeldin S,Fujinaka H,Ismail TA,Yaoita E,Yamamoto T

    更新日期：2011-06-10 00:00:00

  • Urine metabolomic analysis in clear cell and papillary renal cell carcinoma: A pilot study.

    abstract：:Renal cell carcinoma (RCC) is one of the most lethal type of tumors and is twice more frequent in men than in women. Initial symptoms are unspecific and belated thus increasing mortality. Moreover, current diagnostic and monitoring tools are harmful for the patient and unspecific in low-grade tumors. Therefore, novel ...

    journal_title：Journal of proteomics

    pub_type： 杂志文章

    doi：10.1016/j.jprot.2020.103723

    authors： Oto J,Fernández-Pardo Á,Roca M,Plana E,Solmoirago MJ,Sánchez-González JV,Vera-Donoso CD,Martínez-Sarmiento M,España F,Navarro S,Medina P

    更新日期：2020-04-30 00:00:00

  • Comparative proteomic analysis of sex-biased proteins in ovary and testis at different stages of Spodoptera litura.

    abstract：:Sex-biased protein is thought to be able to drive the phenotypic differences in males and females in insects. In this study, 1385 and 1727 proteins were identified as differentially accumulated proteins (DAPs) by comparing the protein abundances at pupae stage with those at adult stage in ovary and testis of S.litura,...

    journal_title：Journal of proteomics

    pub_type： 杂志文章

    doi：10.1016/j.jprot.2019.103439

    authors： Sun R,Sun Z,Chen Y,Zhu F,Li Y,Zhong G,Yi X

    更新日期：2019-08-30 00:00:00

  • Global analysis of ubiquitome in PRRSV-infected pulmonary alveolar macrophages.

    abstract：:Protein lysine ubiquitination is a dynamic reversible post-translational modification that plays key roles in modulating different cellular processes. Porcine reproductive and respiratory syndrome virus (PRRSV) is a notorious pathogen, causing tremendous economic losses for the global swine industry. The possible invo...

    journal_title：Journal of proteomics

    pub_type： 杂志文章

    doi：10.1016/j.jprot.2018.06.010

    authors： Zhang H,Fang L,Zhu X,Wang D,Xiao S

    更新日期：2018-07-30 00:00:00

  • Usefulness of non-enzymatic post-translational modification derived products (PTMDPs) as biomarkers of chronic diseases.

    abstract：:Molecular aging of proteins results from the complex association of different reactions that lead to the progressive alteration of their structural and functional properties. These reactions, which include oxidation, glycoxidation, carbonylation and carbamylation, occur during aging and are amplified in various chroni...

    journal_title：Journal of proteomics

    pub_type： 杂志文章,评审

    doi：10.1016/j.jprot.2013.02.015

    authors： Gillery P,Jaisson S

    更新日期：2013-10-30 00:00:00

  • Protein profiling identified dissociations between growth hormone-mediated longitudinal growth and bone mineralization in short prepubertal children.

    abstract：:Growth hormone (GH) promotes longitudinal growth and bone mineralization. In this study, a proteomic approach was used to analyze the association between serum protein expression pattern and height-adjusted bone mineralization in short prepubertal children receiving GH treatment. Patterns of protein expression were co...

    journal_title：Journal of proteomics

    pub_type： 杂志文章

    doi：10.1016/j.jprot.2010.08.007

    authors： Andersson B,Decker R,Nierop AF,Bosaeus I,Albertsson-Wikland K,Hellgren G

    更新日期：2011-01-01 00:00:00

  • Assessment of BAK1 activity in different plant receptor-like kinase complexes by quantitative profiling of phosphorylation patterns.

    abstract：UNLABELLED:Plant receptor-like kinases (RLKs) constitute a large family of receptors coordinating developmental programs with adaptation to environmental stresses including immune defenses. BRI1-ASSOCIATED KINASE 1 (BAK1), a member of the plant RLK family, forms receptor complexes with multiple RLK proteins including B...

    journal_title：Journal of proteomics

    pub_type： 杂志文章

    doi：10.1016/j.jprot.2014.06.009

    authors： Wang Y,Li Z,Liu D,Xu J,Wei X,Yan L,Yang C,Lou Z,Shui W

    更新日期：2014-08-28 00:00:00

  • Proteomic biomarkers for psoriasis and psoriasis arthritis.

    abstract：UNLABELLED:Although several new biomarkers have been recently proposed for psoriasis (Ps) and psoriasis arthritis (PsA), nothing is known about their diagnostic sensitivity and specificity, and their routine use. We therefore searched in-depth for new biomarker candidates using a biobank with EDTA-plasma from 158 indiv...

    journal_title：Journal of proteomics

    pub_type： 杂志文章

    doi：10.1016/j.jprot.2016.03.040

    authors： Reindl J,Pesek J,Krüger T,Wendler S,Nemitz S,Muckova P,Büchler R,Opitz S,Krieg N,Norgauer J,Rhode H

    更新日期：2016-05-17 00:00:00

  • Plant proteomics update (2007-2008): Second-generation proteomic techniques, an appropriate experimental design, and data analysis to fulfill MIAPE standards, increase plant proteome coverage and expand biological knowledge.

    abstract：:This review is the continuation of three previously published articles [Jorrin JV, Maldonado AM, Castillejo MA. Plant proteome analysis: a 2006 update. Proteomics 2007; 7: 2947-2962; Rossignol M, Peltier JB, Mock HP, Matros A, Maldonado AM, Jorrin JV. Plant proteome analysis: a 2004-2006 update. Proteomics 2006; 6: 55...

    journal_title：Journal of proteomics

    pub_type： 杂志文章,评审

    doi：10.1016/j.jprot.2009.01.026

    authors： Jorrín-Novo JV,Maldonado AM,Echevarría-Zomeño S,Valledor L,Castillejo MA,Curto M,Valero J,Sghaier B,Donoso G,Redondo I

    更新日期：2009-04-13 00:00:00

  • Accurate quantitation of MHC-bound peptides by application of isotopically labeled peptide MHC complexes.

    abstract：:Knowledge of the accurate copy number of HLA class I presented ligands is important in fundamental and clinical immunology. Currently, the best copy number determinations are based on mass spectrometry, employing single reaction monitoring (SRM) in combination with a known amount of isotopically labeled peptide. The m...

    journal_title：Journal of proteomics

    pub_type： 杂志文章

    doi：10.1016/j.jprot.2014.07.009

    authors： Hassan C,Kester MG,Oudgenoeg G,de Ru AH,Janssen GM,Drijfhout JW,Spaapen RM,Jiménez CR,Heemskerk MH,Falkenburg JH,van Veelen PA

    更新日期：2014-09-23 00:00:00

  • Embryonic atrazine exposure elicits proteomic, behavioral, and brain abnormalities with developmental time specific gene expression signatures.

    abstract：:Atrazine (ATZ), the second most commonly used herbicide in the United States, is an endocrine disrupting chemical linked to cancer and a common drinking water contaminant. This study further investigates ATZ-related developmental toxicity by testing the following hypotheses in zebrafish: the effects of embryonic ATZ e...

    journal_title：Journal of proteomics

    pub_type： 杂志文章

    doi：10.1016/j.jprot.2018.07.006

    authors： Horzmann KA,Reidenbach LS,Thanki DH,Winchester AE,Qualizza BA,Ryan GA,Egan KE,Hedrick VE,Sobreira TJP,Peterson SM,Weber GJ,Wirbisky-Hershberger SE,Sepúlveda MS,Freeman JL

    更新日期：2018-08-30 00:00:00

  • Identification of thioredoxin target disulfides in proteins released from barley aleurone layers.

    abstract：:Thioredoxins are ubiquitous disulfide reductases involved in a wide range of cellular processes including DNA synthesis, oxidative stress response and apoptosis. In cereal seeds thioredoxins are proposed to facilitate the germination process by reducing disulfide bonds in storage proteins and other targets in the star...

    journal_title：Journal of proteomics

    pub_type： 杂志文章

    doi：10.1016/j.jprot.2010.01.007

    authors： Hägglund P,Bunkenborg J,Yang F,Harder LM,Finnie C,Svensson B

    更新日期：2010-04-18 00:00:00

  • Identification of differentially activated pathways in Phytophthora sojae at the mycelial, cyst, and oospore stages by TMT-based quantitative proteomics analysis.

    abstract：:Phytophthora sojae is a widely distributed, destructive oomycete plant pathogen that has been developed as a model for oomycete biology. Given the important but limited reports on the comparison of the sexual and asexual stages in Phytophthora, we performed a large-scale quantitative proteomics study on two key asexua...

    journal_title：Journal of proteomics

    pub_type： 杂志文章

    doi：10.1016/j.jprot.2020.103776

    authors： Zhang C,Cui T,Zhang F,Xue Z,Miao J,Wang W,Liu X

    更新日期：2020-06-15 00:00:00

  • Design and expression of a QconCAT protein to validate Hi3 protein quantification of influenza vaccine antigens.

    abstract：UNLABELLED:Quantification of the antigens hemagglutinin and neuraminidase in influenza vaccines has been reported using an antibody-free liquid chromatography-mass spectrometry (LC-MS) based method known as MS(E) "Hi3". This approach is based on the average signal intensity of the three most intense tryptic peptides re...

    journal_title：Journal of proteomics

    pub_type： 杂志文章

    doi：10.1016/j.jprot.2016.06.024

    authors： Smith DG,Gingras G,Aubin Y,Cyr TD

    更新日期：2016-09-02 00:00:00

  • Serological autoantibody profiling of type 1 diabetes by protein arrays.

    abstract：UNLABELLED:The need for biomarkers that illuminate the pathophysiology of type 1 diabetes (T1D), enhance early diagnosis and provide additional avenues for therapeutic intervention is well recognized in the scientific community. We conducted a proteome-scale, two-stage serological AAb screening followed by an independe...

    journal_title：Journal of proteomics

    pub_type： 杂志文章,多中心研究,随机对照试验

    doi：10.1016/j.jprot.2013.10.018

    authors： Miersch S,Bian X,Wallstrom G,Sibani S,Logvinenko T,Wasserfall CH,Schatz D,Atkinson M,Qiu J,LaBaer J

    更新日期：2013-12-06 00:00:00