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Usage of electrostatic eliminator reduces human keratin contamination significantly in gel-based proteomics analysis.

Abstract：

:In the field of bottom-up proteomics, heavy contamination of human keratins could hinder the comprehensive protein identification, especially for the detection of low abundance proteins. In this study, we examined the keratin contamination in the four major experimental procedures in gel-based proteomic analysis including gel preparation, gel electrophoresis, gel staining, and in-gel digestion. We found that in-gel digestion procedure might be of importance corresponding to keratin contaminants compared to the other three ones. The human keratin contamination was reduced significantly by using an electrostatic eliminator during in-gel digestion, suggesting that static electricity built up on insulated experimental materials might be one of the essential causes of keratin contamination. We herein proposed a series of methods for improving experimental conditions and sample treatment in order to minimize the keratin contamination in an economical and practical way.

journal_name

J Proteomics

journal_title

Journal of proteomics

authors

Xu B,Zhang Y,Zhao Z,Yoshida Y,Magdeldin S,Fujinaka H,Ismail TA,Yaoita E,Yamamoto T

doi

10.1016/j.jprot.2011.03.001

subject

Has Abstract

pub_date

2011-06-10 00:00:00

pages

1022-9

issue

7

eissn

1874-3919

issn

1876-7737

pii

S1874-3919(11)00091-1

journal_volume

74

pub_type

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