Abstract：
:A comparative proteomic approach, using two dimensional gel electrophoresis and mass spectrometry, has been developed to compare and elucidate the differences among the cellular proteomes of four closely related isogenic O/C, SIN, N/R and T, B. clausii strains during both exponential and stationary phases of growth. Image analysis of the electropherograms reveals a high degree of concordance among the four proteomes, some proteins result, however, differently expressed. The proteins spots exhibiting high different expression level were identified, by mass-spectrometry analysis, as alcohol dehydrogenase (ADHA, EC1.2.1.3; ABC0046 isoform) aldehyde dehydrogenase (DHAS, EC 1.2.1.3; ABC0047 isoform) and flagellin-protein of B. clausii KSM-k16. The different expression levels of the two dehydrogenases were confirmed by quantitative RT-PCR and dehydrogenases enzymatic activity. The different patterns of protein expression can be considered as cell proteome signatures of the different strains.
journal_name
J Proteomicsjournal_title
Journal of proteomicsauthors
Lippolis R,Gnoni A,Abbrescia A,Panelli D,Maiorano S,Paternoster MS,Sardanelli AM,Papa S,Gaballo Adoi
10.1016/j.jprot.2011.06.032subject
Has Abstractpub_date
2011-11-18 00:00:00pages
2846-55issue
12eissn
1874-3919issn
1876-7737pii
S1874-3919(11)00295-8journal_volume
74pub_type
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