Characterizations and proteome analysis of platelet-free plasma-derived microparticles in β-thalassemia/hemoglobin E patients.

Abstract:

:Aggregatability and oxidative damage of red blood cells (RBCs), platelet activation and increased amount of blood cells-derived microparticles (MPs) are thought to be the etiologies for the thrombotic risk in thalassemia, but with unclear mechanisms. Here we report cellular origins and increases in number, oxidative stress status, and procoagulant activity, as well as altered proteome of MPs isolated from β-thal/HbE patients. Flow cytometric analysis revealed that β-thal/HbE patients had significantly higher levels of phosphatidylserine (PS)-bearing MPs in platelet-free plasma (PFP) as compared to normal subjects. The high levels of MPs correlated with not only the increased procoagulant activity but also the increased platelet counts. Additionally, these PS-bearing MPs were originated mostly from platelets and RBCs, both of which had increased levels of reactive oxygen species. Proteome analysis of MPs by 2-DE followed by Q-TOF MS and MS/MS analyses identified 29 proteins with significantly altered levels in MPs derived from β-thal/HbE patients (e.g. the increased levels of peroxiredoxin 6, apolipoprotein E, cyclophilin A and heat shock protein 90). These findings suggest that the oxidative damage in platelets and RBCs potentially induces production of MPs with altered proteome that may, in turn, facilitate thromboembolic complications, which are commonly found in thalassemic patients. This article is part of a Special Issue entitled: Integrated omics.

journal_name

J Proteomics

journal_title

Journal of proteomics

authors

Chaichompoo P,Kumya P,Khowawisetsut L,Chiangjong W,Chaiyarit S,Pongsakul N,Sirithanaratanakul N,Fucharoen S,Thongboonkerd V,Pattanapanyasat K

doi

10.1016/j.jprot.2012.06.004

subject

Has Abstract

pub_date

2012-12-05 00:00:00

pages

239-50

eissn

1874-3919

issn

1876-7737

pii

S1874-3919(12)00428-9

journal_volume

76 Spec No.

pub_type

临床试验,杂志文章
  • Label-free quantitative proteomics trends for protein-protein interactions.

    abstract::Understanding protein interactions within the complexity of a living cell is challenging, but techniques coupling affinity purification and mass spectrometry have enabled important progress to be made in the past 15 years. As identification of protein-protein interactions is becoming easier, the quantification of the ...

    journal_title:Journal of proteomics

    pub_type: 杂志文章,评审

    doi:10.1016/j.jprot.2012.10.027

    authors: Tate S,Larsen B,Bonner R,Gingras AC

    更新日期:2013-04-09 00:00:00

  • Comparative leaf proteomics of drought-tolerant and -susceptible peanut in response to water stress.

    abstract:UNLABELLED:Water stress (WS) predisposes peanut plants to fungal infection resulting in pre-harvest aflatoxin contamination. Major changes during water stress including oxidative stress, lead to destruction of photosynthetic apparatus and other macromolecules within cells. Two peanut cultivars with diverse drought tole...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2016.05.031

    authors: Katam R,Sakata K,Suravajhala P,Pechan T,Kambiranda DM,Naik KS,Guo B,Basha SM

    更新日期:2016-06-30 00:00:00

  • iTRAQ-based quantitative proteomic analysis reveals multiple effects of Emodin to Haemophilus parasuis.

    abstract::Haemophilus parasuis, a symbiotic bacteria of upper respiratory tract of swine, is the etiological agent of Glässer's disease, which is characterized by fibrinous polyserositis. Emodin, exhibits antibacterial activity against H. parasuis, yet the action mode has not been fully understood. In present study, isobaric ta...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2017.06.020

    authors: Li L,Tian Y,Yu J,Song X,Jia R,Cui Q,Tong W,Zou Y,Li L,Yin L,Liang X,He C,Yue G,Ye G,Zhao L,Shi F,Lv C,Cao S,Yin Z

    更新日期:2017-08-23 00:00:00

  • Identification of differentially activated pathways in Phytophthora sojae at the mycelial, cyst, and oospore stages by TMT-based quantitative proteomics analysis.

    abstract::Phytophthora sojae is a widely distributed, destructive oomycete plant pathogen that has been developed as a model for oomycete biology. Given the important but limited reports on the comparison of the sexual and asexual stages in Phytophthora, we performed a large-scale quantitative proteomics study on two key asexua...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2020.103776

    authors: Zhang C,Cui T,Zhang F,Xue Z,Miao J,Wang W,Liu X

    更新日期:2020-06-15 00:00:00

  • Secernin-1 as a novel prognostic biomarker candidate of synovial sarcoma revealed by proteomics.

    abstract::We aimed to develop prognostic biomarkers for synovial sarcoma employing a proteomic approach. We examined the proteomic profile of synovial sarcoma using two-dimensional difference gel electrophoresis (2D-DIGE). We identified 20 protein spots whose intensity was statistically different (p<0.01) between a group of eig...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2011.02.033

    authors: Suehara Y,Tochigi N,Kubota D,Kikuta K,Nakayama R,Seki K,Yoshida A,Ichikawa H,Hasegawa T,Kaneko K,Chuman H,Beppu Y,Kawai A,Kondo T

    更新日期:2011-05-16 00:00:00

  • Quantitative proteomics of the yeast Hsp70/Hsp90 interactomes during DNA damage reveal chaperone-dependent regulation of ribonucleotide reductase.

    abstract:UNLABELLED:The highly conserved molecular chaperones Hsp90 and Hsp70 are indispensible for folding and maturation of a significant fraction of the proteome, including many proteins involved in signal transduction and stress response. To examine the dynamics of chaperone-client interactions after DNA damage, we applied ...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2014.09.028

    authors: Truman AW,Kristjansdottir K,Wolfgeher D,Ricco N,Mayampurath A,Volchenboum SL,Clotet J,Kron SJ

    更新日期:2015-01-01 00:00:00

  • Comparative proteomic analysis of BTH and BABA-induced resistance in pea (Pisum sativum) toward infection with pea rust (Uromyces pisi).

    abstract::Systemic acquired resistance (SAR) to Uromyces pisi in pea was studied by using a proteomic approach. Two-dimensional electrophoresis (2-DE) was used in order to compare the leaf proteome of two pea genotypes displaying different phenotypes (susceptible and partial resistance to the fungus), and in response to parasit...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2012.06.033

    authors: Barilli E,Rubiales D,Castillejo MÁ

    更新日期:2012-09-18 00:00:00

  • Structural characterisation of medically relevant protein assemblies by integrating mass spectrometry with computational modelling.

    abstract::Structural mass spectrometry with its various techniques is a powerful tool for the structural elucidation of medically relevant protein assemblies. It delivers information on the composition, stoichiometries, interactions and topologies of these assemblies. Most importantly it can deal with heterogeneous mixtures and...

    journal_title:Journal of proteomics

    pub_type: 杂志文章,评审

    doi:10.1016/j.jprot.2017.04.019

    authors: Politis A,Schmidt C

    更新日期:2018-03-20 00:00:00

  • Construction of nanometer cisplatin core-ferritin (NCC-F) and proteomic analysis of gastric cancer cell apoptosis induced with cisplatin released from the NCC-F.

    abstract::Both transmission electron microscopy (TEM) and fluorescence spectrometry were used to reveal the characteristics of both subunit disassociation and recombination in apo-pig pancreas ferritin (apoPPF) in an alkaline medium ranging reversibly from pH 7.0 to 13.0. The experimental results indicated that apoPPF could be ...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2012.03.013

    authors: Ji XT,Huang L,Huang HQ

    更新日期:2012-06-18 00:00:00

  • Urine metabolomic analysis in clear cell and papillary renal cell carcinoma: A pilot study.

    abstract::Renal cell carcinoma (RCC) is one of the most lethal type of tumors and is twice more frequent in men than in women. Initial symptoms are unspecific and belated thus increasing mortality. Moreover, current diagnostic and monitoring tools are harmful for the patient and unspecific in low-grade tumors. Therefore, novel ...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2020.103723

    authors: Oto J,Fernández-Pardo Á,Roca M,Plana E,Solmoirago MJ,Sánchez-González JV,Vera-Donoso CD,Martínez-Sarmiento M,España F,Navarro S,Medina P

    更新日期:2020-04-30 00:00:00

  • Validation of serum protein profiles by a dual antibody array approach.

    abstract::In recent years, affinity-based technologies have become important tools for serum profiling to uncover protein expression patterns linked to disease state or therapeutic effects. In this study, we describe a path towards the production of an antibody microarray to allow protein profiling of biotinylated human serum s...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2009.09.009

    authors: Rimini R,Schwenk JM,Sundberg M,Sjöberg R,Klevebring D,Gry M,Uhlén M,Nilsson P

    更新日期:2009-12-01 00:00:00

  • Quantitative secretome analysis unravels new secreted proteins in Amphotericin B resistant Leishmania donovani.

    abstract::Leishmaniasis is second most neglected disease after malaria and seems to be a worldwide concern because of increased drug resistance and non-availability of approved vaccine. The underlying molecular mechanism of drug resistance (Amp B) in Leishmania parasites still remains elusive. Herein, the present study investig...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2019.103464

    authors: Garg G,Ali V,Singh K,Gupta P,Ganguly A,Sahasrabuddhe AA,Das P

    更新日期:2019-09-15 00:00:00

  • Technical report: Targeted proteomic analysis reveals enrichment of atypical ubiquitin chains in contractile murine tissues.

    abstract::Ubiquitylation is an elaborate post-translational modification involved in all biological processes. Its pleotropic effect is driven by the ability to form complex polyubiquitin chain architectures that can influence biological functions. In this study, we optimised sample preparation and chromatographic separation of...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2020.103963

    authors: Heunis T,Lamoliatte F,Marín-Rubio JL,Dannoura A,Trost M

    更新日期:2020-10-30 00:00:00

  • A quantitation module for isotope-labeled peptides integrated into PatternLab for proteomics.

    abstract::We present a new module integrated into the widely adopted PatternLab for proteomics to enable analysis of isotope-labeled peptides produced using dimethyl or SILAC. The accurate quantitation of proteins lies within the heart of proteomics; dimethylation has shown to be reliable, inexpensive, and applicable to any sam...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2019.04.021

    authors: Santos MDM,Lima DB,Silva ARF,Kurt LU,Clasen MA,Pinto AFM,Moresco JJ,Yates JR 3rd,Aquino P,Barbosa VC,Fischer JSG,Carvalho PC

    更新日期:2019-06-30 00:00:00

  • Exosomes derived from calcium oxalate-exposed macrophages enhance IL-8 production from renal cells, neutrophil migration and crystal invasion through extracellular matrix.

    abstract::Deposition of calcium oxalate (CaOx) crystals in renal interstitium is one of the key factors that cause progressive inflammation in kidney stone disease. Macrophages are responsible for elimination of these crystals but their roles to worsen inflammatory process remain under-investigated. This study thus aimed to def...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2018.06.015

    authors: Singhto N,Thongboonkerd V

    更新日期:2018-08-15 00:00:00

  • Neuronal porosome proteome: Molecular dynamics and architecture.

    abstract::Porosomes are the universal secretory portals at the cell plasma membrane, where membrane-bound secretory vesicles transiently dock and fuse to expel intravesicular contents to the outside during cell secretion. In the past decade, the neuronal porosome complex, a 10-15nm cup-shaped lipoprotein structure has been isol...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2012.05.017

    authors: Lee JS,Jeremic A,Shin L,Cho WJ,Chen X,Jena BP

    更新日期:2012-07-16 00:00:00

  • Proteomics of wine additives: mining for the invisible via combinatorial peptide ligand libraries.

    abstract::Combinatorial peptide ligand libraries (CPLLs) have been adopted for harvesting and identifying traces of casein (used as a fining agent) present in white wines. Although minute amounts (200 microL) of CPLL beads are added to the entire content of a wine bottle (750 mL), they are able to sequester with high efficiency...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2010.05.010

    authors: Cereda A,Kravchuk AV,D'Amato A,Bachi A,Righetti PG

    更新日期:2010-08-05 00:00:00

  • A comparative proteomic approach to better define Deinococcus nucleoid specificities.

    abstract::Compared to radiation-sensitive bacteria, the nucleoids of radiation-resistant Deinococcus species show a higher degree of compaction. Such a condensed nucleoid may contribute to the extreme radiation resistance of Deinococcus by limiting dispersion of radiation-induced DNA fragments. Architectural proteins may play a...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2012.03.002

    authors: Toueille M,Mirabella B,Guérin P,Bouthier de la Tour C,Boisnard S,Nguyen HH,Blanchard L,Servant P,de Groot A,Sommer S,Armengaud J

    更新日期:2012-05-17 00:00:00

  • Comparative proteome analyses of host protein expression in response to Enterovirus 71 and Coxsackievirus A16 infections.

    abstract::Enterovirus 71 (EV71) and Coxsackievirus A16 (CA16) are the main etiological agents of Hand, Foot and Mouth Disease (HFMD), a common disease among children and had caused several outbreaks in the Asia-Pacific region. Although being genetically close to each other, EV71 infection can cause serious and fatal neurologica...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2011.05.022

    authors: Lee JJ,Seah JB,Chow VT,Poh CL,Tan EL

    更新日期:2011-09-06 00:00:00

  • Non-additive effects of ocean acidification in combination with warming on the larval proteome of the Pacific oyster, Crassostrea gigas.

    abstract:UNLABELLED:Increasing atmospheric carbon dioxide results in ocean acidification and warming, significantly impacting marine invertebrate larvae development. We investigated how ocean acidification in combination with warming affected D-veliger larvae of the Pacific oyster Crassostrea gigas. Larvae were reared for 40h u...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2015.12.001

    authors: Harney E,Artigaud S,Le Souchu P,Miner P,Corporeau C,Essid H,Pichereau V,Nunes FLD

    更新日期:2016-03-01 00:00:00

  • Transcriptome-facilitated proteomic characterization of rear-fanged snake venoms reveal abundant metalloproteinases with enhanced activity.

    abstract::High-throughput technologies were used to identify venom gland toxin expression and to characterize the venom proteomes of two rear-fanged snakes, Ahaetulla prasina (Asian Green Vine Snake) and Borikenophis portoricensis (Puerto Rican Racer). Sixty-nine complete toxin-coding transcripts from 12 venom protein superfami...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2018.08.004

    authors: Modahl CM,Frietze S,Mackessy SP

    更新日期:2018-09-15 00:00:00

  • Proteomics on porcine haptoglobin and IgG/IgA show protein species distribution and glycosylation pattern to remain similar in PCV2-SD infection.

    abstract:UNLABELLED:Haptoglobin (Hp) and immunoglobulins are plasma glycoproteins involved in the immune reaction of the organism after infection and/or inflammation. Porcine circovirus type 2-systemic disease (PCV2-SD), formerly known as postweaning multisystemic wasting syndrome (PMWS), is a globally spread pig disease of gre...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2014.02.018

    authors: Marco-Ramell A,Miller I,Nöbauer K,Möginger U,Segalés J,Razzazi-Fazeli E,Kolarich D,Bassols A

    更新日期:2014-04-14 00:00:00

  • A reflection on the role of ICP-MS in proteomics: Update and future perspective.

    abstract::Mass spectrometry is the technique of reference for the identification and quantification of proteins. Whereas ESI and MALDI ionization sources are inherently not quantitative being highly influenced by the chemical nature of the analyte and the matrix, ICP-MS uses a hard ionization source that destroys proteins into ...

    journal_title:Journal of proteomics

    pub_type: 杂志文章,评审

    doi:10.1016/j.jprot.2018.11.010

    authors: Calderón-Celis F,Encinar JR

    更新日期:2019-04-30 00:00:00

  • Identification of novel interacting partners of the NEDD4 ubiquitin ligase in mouse testis.

    abstract::Posttranslational modification by ubiquitination targets proteins for degradation, recycling, stabilization or altered trafficking, and as such can alter cellular signaling pathways. The substrate specificity of this multistep process is controlled by ubiquitin ligases, including those of the HECT domain-containing NE...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2020.103830

    authors: Manning J,Windley SP,Sandow JJ,Shah SS,Western P,Wilhelm D,Kumar S

    更新日期:2020-07-15 00:00:00

  • Comparative evaluation of electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) and high-pH reversed phase (Hp-RP) chromatography in profiling of rat kidney proteome.

    abstract:UNLABELLED:ERLIC and high-pH RP (Hp-RP) have been reported to be promising alternatives to strong cation exchange (SCX) in proteome fractionation. Here we compared the performance of ERLIC, concatenated ERLIC and concatenated Hp-RP in proteome profiling. The protein identification is comparable in these three strategie...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2013.02.008

    authors: Hao P,Ren Y,Dutta B,Sze SK

    更新日期:2013-04-26 00:00:00

  • A Fusarium graminearum strain-comparative proteomic approach identifies regulatory changes triggered by agmatine.

    abstract:UNLABELLED:Plant pathogens face different environmental clues depending on the stage of the infection cycle they are in. Fusarium graminearum infects small grain cereals producing trichothecenes type B (TB) that act as virulence factor in the interaction with the plant and have important food safety implications. This ...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2015.11.010

    authors: Pasquali M,Serchi T,Cocco E,Leclercq CC,Planchon S,Guignard C,Renaut J,Hoffmann L

    更新日期:2016-03-30 00:00:00

  • Profiling the short, linear, non-disulfide bond-containing peptidome from the venom of the scorpion Tityus obscurus.

    abstract::Many scorpion accidents occur in the Brazilian Amazonian region and are frequently caused by Tityus obscurus. Approximately 5% of the crude venom of this species is composed of short linear, non-disulfide-bridged peptides, which have not been intensively investigated. As a consequence, only a few of these peptides hav...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2017.09.006

    authors: Dias NB,de Souza BM,Cocchi FK,Chalkidis HM,Dorce VAC,Palma MS

    更新日期:2018-01-06 00:00:00

  • Integrated physiological and proteomic analysis reveals underlying response and defense mechanisms of Brachypodium distachyon seedling leaves under osmotic stress, cadmium and their combined stresses.

    abstract::Drought stress, a major abiotic stress, commonly occurs in metal-contaminated environments and affects crop growth and yield. In this study, we performed the first integrated phenotypic, physiological, and proteomic analysis of Brachypodium distachyon L. seedling leaves under polyethylene glycol (PEG) mock osmotic str...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2017.09.015

    authors: Cheng ZW,Chen ZY,Yan X,Bian YW,Deng X,Yan YM

    更新日期:2018-01-06 00:00:00

  • Identification of plasma-responsive outer membrane proteins and their vaccine potential in Edwardsiella tarda using proteomic approach.

    abstract::We have used differential sub-proteomic methodologies to detect Edwardsiella tarda outer membrane (OM) protein expression regulation during interaction with fish and human plasma, which is the critical step of the bacterial invasion internal organs via blood circulation. Seven and nine OM proteins were differentially ...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2011.11.001

    authors: Wang C,Liu Y,Li H,Xu WJ,Zhang H,Peng XX

    更新日期:2012-02-02 00:00:00

  • Internal calibrants allow high accuracy peptide matching between MALDI imaging MS and LC-MS/MS.

    abstract::One of the important challenges for MALDI imaging mass spectrometry (MALDI-IMS) is the unambiguous identification of measured analytes. One way to do this is to match tryptic peptide MALDI-IMS m/z values with LC-MS/MS identified m/z values. Matching using current MALDI-TOF/TOF MS instruments is difficult due to the va...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2012.04.054

    authors: Gustafsson JOR,Eddes JS,Meding S,Koudelka T,Oehler MK,McColl SR,Hoffmann P

    更新日期:2012-08-30 00:00:00