Abstract:
:Aggregatability and oxidative damage of red blood cells (RBCs), platelet activation and increased amount of blood cells-derived microparticles (MPs) are thought to be the etiologies for the thrombotic risk in thalassemia, but with unclear mechanisms. Here we report cellular origins and increases in number, oxidative stress status, and procoagulant activity, as well as altered proteome of MPs isolated from β-thal/HbE patients. Flow cytometric analysis revealed that β-thal/HbE patients had significantly higher levels of phosphatidylserine (PS)-bearing MPs in platelet-free plasma (PFP) as compared to normal subjects. The high levels of MPs correlated with not only the increased procoagulant activity but also the increased platelet counts. Additionally, these PS-bearing MPs were originated mostly from platelets and RBCs, both of which had increased levels of reactive oxygen species. Proteome analysis of MPs by 2-DE followed by Q-TOF MS and MS/MS analyses identified 29 proteins with significantly altered levels in MPs derived from β-thal/HbE patients (e.g. the increased levels of peroxiredoxin 6, apolipoprotein E, cyclophilin A and heat shock protein 90). These findings suggest that the oxidative damage in platelets and RBCs potentially induces production of MPs with altered proteome that may, in turn, facilitate thromboembolic complications, which are commonly found in thalassemic patients. This article is part of a Special Issue entitled: Integrated omics.
journal_name
J Proteomicsjournal_title
Journal of proteomicsauthors
Chaichompoo P,Kumya P,Khowawisetsut L,Chiangjong W,Chaiyarit S,Pongsakul N,Sirithanaratanakul N,Fucharoen S,Thongboonkerd V,Pattanapanyasat Kdoi
10.1016/j.jprot.2012.06.004subject
Has Abstractpub_date
2012-12-05 00:00:00pages
239-50eissn
1874-3919issn
1876-7737pii
S1874-3919(12)00428-9journal_volume
76 Spec No.pub_type
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journal_title:Journal of proteomics
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journal_title:Journal of proteomics
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journal_title:Journal of proteomics
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journal_title:Journal of proteomics
pub_type: 杂志文章
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journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2010.05.010
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journal_title:Journal of proteomics
pub_type: 杂志文章
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journal_title:Journal of proteomics
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journal_title:Journal of proteomics
pub_type: 杂志文章,评审
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journal_title:Journal of proteomics
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journal_title:Journal of proteomics
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更新日期:2016-03-30 00:00:00
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journal_title:Journal of proteomics
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journal_title:Journal of proteomics
pub_type: 杂志文章
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更新日期:2012-02-02 00:00:00
abstract::One of the important challenges for MALDI imaging mass spectrometry (MALDI-IMS) is the unambiguous identification of measured analytes. One way to do this is to match tryptic peptide MALDI-IMS m/z values with LC-MS/MS identified m/z values. Matching using current MALDI-TOF/TOF MS instruments is difficult due to the va...
journal_title:Journal of proteomics
pub_type: 杂志文章
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更新日期:2012-08-30 00:00:00