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Experimental model of isolated lung perfusion in rats: first Brazilian experience using the IL-2 isolated perfused rat or guinea pig lung system.

Abstract：

INTRODUCTION:Lung transplantation has become the mainstay therapy for patients with end-stage lung disease refractory to medical management. However, the number of patients listed for lung transplantation largely exceeds available donors. The study of lung preservation requires accurate, cost-effective small animal models. We have described a model of ex vivo rat lung perfusion using a commercially available system. METHODS:Male Wistar rats weighing 250 g-300 g were anesthetized with intraperitoneal sodium thiopental (50 mg/kg body weight). The surgical technique included heart-lung block extraction, assembly, and preparation for perfusion and data collection. We used an IL-2 Isolated Perfused Rat or Guinea Pig Lung System (Harvard Apparatus, Holliston, Mass, United States; Hugo Sachs Elektronik, Alemanha). RESULTS:Preliminary results included hemodynamic and pulmonary mechanics data gathered in the experiments. CONCLUSION:The isolated rat lung perfusion system is a reliable method to assess lung preservation.

journal_name

Transplant Proc

journal_title

Transplantation proceedings

authors

Pêgo-Fernandes PM,Werebe E,Cardoso PF,Pazetti R,de Oliveira KA,Soares PR,Jatene FB

doi

10.1016/j.transproceed.2010.01.016

subject

Has Abstract

pub_date

2010-03-01 00:00:00

pages

444-7

issue

2

eissn

0041-1345

issn

1873-2623

pii

S0041-1345(10)00106-5

journal_volume

42

pub_type

杂志文章

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