Abstract:
:About 30 years ago circulating endothelial cells (CEC) were first observed in peripheral blood. Since then CEC have been established as a reliable indicator of vascular injury and damage and more sophisticated detection techniques, such as immunomagnetic isolation and fluorescence-activated cell sorting (FACS), have become available. However even today there remains controversy as to the best approach to isolate and enumerate these cells. Here, we review the isolation and enumeration of CEC with an emphasis on CD146-driven immunomagnetic isolation and FACS as the two competing techniques. We describe advantages and pitfalls of both approaches. Moreover, we provide a list of clinical studies in this field and describe the possible clinical utility of CEC as a surrogate marker for vascular damage and dysfunction. In addition, we review the phenotype of CEC and discuss mechanisms of detachment. Recent evidence has also revealed interesting interactions between CEC and healthy endothelium in vitro although the relevance of these findings for human vascular disease in vivo remains unclear. Finally, we highlight differences between circulating endothelial cells and endothelial progenitor cells. In summary, CEC must be regarded as a sensitive and specific marker of endothelial damage as well as a potential mediator in vascular disease.
journal_name
Curr Stem Cell Res Therjournal_title
Current stem cell research & therapyauthors
Erdbruegger U,Dhaygude A,Haubitz M,Woywodt Adoi
10.2174/157488810793351721subject
Has Abstractpub_date
2010-12-01 00:00:00pages
294-302issue
4eissn
1574-888Xissn
2212-3946pii
ABSTRACT # 66journal_volume
5pub_type
杂志文章,评审abstract::Heterogeneity is a well-documented phenomenon in breast cancer; one of the explanations for this phenomenon is the presence of cancer stem cells (CSCs) with the capacity to differentiate along divergent pathways. These CSCs undergo asymmetric and symmetric division resulting in both expansion of the stem cell pool and...
journal_title:Current stem cell research & therapy
pub_type: 杂志文章,评审
doi:10.2174/157488809787169110
更新日期:2009-01-01 00:00:00
abstract::In contrast to their almost unlimited potential for expansion in vivo and despite years of dedicated research and optimization of expansion protocols, the expansion of Hematopoietic Stem Cells (HSCs) in vitro remains remarkably limited. Increased understanding of the mechanisms that are involved in maintenance, expans...
journal_title:Current stem cell research & therapy
pub_type: 杂志文章
doi:10.2174/1574888X15666200225091339
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abstract::Mesenchymal stem cells are multipotent stromal cells residing within the connective tissue of most organs. Their surface phenotype has been well described. Most commonly, mesenchymal stem cells demonstrate the ability to differentiate into mesenchymal tissues (bone, catailge, fat, etc...), however, under the proper co...
journal_title:Current stem cell research & therapy
pub_type: 杂志文章,评审
doi:10.2174/157488812799218983
更新日期:2012-03-01 00:00:00
abstract::Intervertebral disc (IVD) disorders, especially degenerative disc disease, reduce the quality of life, and are short of effective therapy. A new direction for treatment of chronic tissue and organ diseases is to promote regeneration by harnessing endogenous repair mechanisms. In this review, we discuss the potential o...
journal_title:Current stem cell research & therapy
pub_type: 杂志文章,评审
doi:10.2174/1574888x10666150305105114
更新日期:2015-01-01 00:00:00
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journal_title:Current stem cell research & therapy
pub_type: 杂志文章,评审
doi:10.2174/1574888X14666191029104249
更新日期:2020-01-01 00:00:00
abstract::Adipose-derived stem cells are adult stem cells which are easy to obtain and multi-potent. Stem-cell therapy has become a promising new treatment for many diseases, and plays an increasingly important role in the field of tissue repair, regeneration and reconstruction. The physicochemical properties of the extracellul...
journal_title:Current stem cell research & therapy
pub_type: 杂志文章
doi:10.2174/1574888X15666200502000343
更新日期:2021-01-01 00:00:00
abstract::Angiogenesis is a complex process in which capillaries are produced from blood vessels that already exists. Endothelial cells (ECs) and endothelial progenitor cells (EPCs) are pivotal for this process and for the maintenance/restorage of the endothelium. Decreased numbers and dysfunction of these cells have been relat...
journal_title:Current stem cell research & therapy
pub_type: 杂志文章,评审
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更新日期:2016-01-01 00:00:00
abstract::Umbilical cord blood (UCB) is an alternative source of hematopoietic progenitors for transplantation in the treatment of haematological malignancies, marrow failure, immunodeficiencies, hemoglobinopathies and inherited metabolic diseases. It has greatly contributed to increase the feasibility to transplantation for ma...
journal_title:Current stem cell research & therapy
pub_type: 杂志文章,评审
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更新日期:2010-12-01 00:00:00
abstract:BACKGROUND:Acute renal failure (ARF) resulting from ischemic or toxic insults remains a major health care problem because of its grave prognosis and the limited effectiveness of available treatment modalities. Current treatment options for ARF are limited to supportive measures and preventive strategies, none of which ...
journal_title:Current stem cell research & therapy
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doi:10.2174/157488811797904344
更新日期:2011-12-01 00:00:00
abstract:BACKGROUND:Revascularisation therapy is the current gold standard of care for critical limb ischemia (CLI), although a significant proportion of patients with CLI either are not fit for or do not respond well to this procedure. Recently, novel angiogenic therapies such as the use of autologous cellbased therapy (CBT) h...
journal_title:Current stem cell research & therapy
pub_type: 杂志文章,meta分析,评审
doi:10.2174/1574888X13666180313141416
更新日期:2018-01-01 00:00:00
abstract::Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease caused by the selective loss of both spinal and upper motor neurons. One strategy in treating ALS is to use stem cells to replace lost spinal motor neurons. However, transplanted stem cell-derived motor neurons may not survive when exposed to the harsh...
journal_title:Current stem cell research & therapy
pub_type: 杂志文章,评审
doi:10.2174/157488809789057392
更新日期:2009-09-01 00:00:00
abstract::Osteoarthritis (OA) is the most common degenerative joint disorder worldwide. OA represents an increasing threat to the quality of life of affected persons as well as for health resources expenditure. The incapability of cartilage to heal has been long time regarded as the major cause of progressive joint degeneration...
journal_title:Current stem cell research & therapy
pub_type: 杂志文章,评审
doi:10.2174/1574888x09666140630102202
更新日期:2015-01-01 00:00:00
abstract::Exosomes and biomimetic nanoparticles have great potential to develop into a wide-scale therapeutic platform within the regenerative medicine industry. Exosomes, a subgroup of EVs with diameter ranging from 30-100 nm, have recently gained attention as an innovative approach for the treatment of various diseases, inclu...
journal_title:Current stem cell research & therapy
pub_type: 杂志文章
doi:10.2174/1574888X15666200309143924
更新日期:2020-01-01 00:00:00
abstract::Tendon and ligament injuries are common, and repair slowly with reduced biomechanical properties. With increasing financial demands on the health service and patients to recover from tendon and ligament injuries faster, and with less morbidity, health professionals are exploring new treatment options. Tissue engineeri...
journal_title:Current stem cell research & therapy
pub_type: 杂志文章,评审
doi:10.2174/1574888x09666140710102808
更新日期:2015-01-01 00:00:00
abstract::The discovery of neural stem cells (NSC) which ensure continuous neurogenesis in the adult mammalian brain, has led to a conceptual revolution in basic neuroscience and to high hopes for clinical nervous tissue repair. However, several research issues remain to address before neural stem cells can be harnessed for reg...
journal_title:Current stem cell research & therapy
pub_type: 杂志文章,评审
doi:10.2174/157488808785740334
更新日期:2008-09-01 00:00:00
abstract::Imbalance between free radicals and antioxidants causes oxidative stress by the accumulation of reactive oxygen species (ROS) in the tissues and organs. Oxidative stress occurs in many damage conditions, and the increase of ROS and reduction of antioxidants enhances inflammation, apoptosis, fibrosis and may worsen the...
journal_title:Current stem cell research & therapy
pub_type: 杂志文章
doi:10.2174/1574888X16999201124153753
更新日期:2020-11-24 00:00:00
abstract::Combination antiretroviral therapy (cART) has significantly reduced the mortality rate and morbidity, and has increased the life expectancy of the human immunodeficiency virus (HIV) infected patients. However, the current cART is incapable of eradicating viruses from the human body, and HIV remains one of the most not...
journal_title:Current stem cell research & therapy
pub_type: 杂志文章,评审
doi:10.2174/1574888X14666190523094556
更新日期:2019-01-01 00:00:00
abstract:BACKGROUND:Today, coronary artery disease is still one of the most important causes of mortality despite advanced surgical methods, pharmacotherapies and organ transplantation. These treatment modalities are intended to prevent further progression of myocardial infarction and do not involve the repair of the damaged pa...
journal_title:Current stem cell research & therapy
pub_type: 杂志文章,评审
doi:10.2174/1574888X13666180510110055
更新日期:2018-01-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) are stromal origin cells with multilineage differentiation capacity. The immunoregulatory properties of MSCs can be interfered effectively by cytokines. Cytokines, produced by a broad range of cells, act at the systemic level to influence biological phenomena such as inflammation, wound h...
journal_title:Current stem cell research & therapy
pub_type: 杂志文章,评审
doi:10.2174/1574888X11666160614103404
更新日期:2017-01-01 00:00:00
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journal_title:Current stem cell research & therapy
pub_type: 杂志文章,评审
doi:10.2174/1574888X14666190429143734
更新日期:2019-01-01 00:00:00
abstract::Notch signaling mediates the fates of numerous cells in both invertebrates and vertebrates. In the immune system, Notch signalling contributes to the generation of hematopoietic stem cells (HSCs), the promotion of HSC self-renewal, T lineage commitment, intrathymic T cell development, and peripheral lymphocyte differe...
journal_title:Current stem cell research & therapy
pub_type: 杂志文章,评审
doi:10.2174/157488811795495459
更新日期:2011-06-01 00:00:00
abstract::Stem cells are undifferentiated cells with the ability to proliferate and convert to different types of differentiated cells that make up the various tissues and organs in the body. They exist both in embryos as pluripotent stem cells that can differentiate into the three germ layers and as multipotent or unipotent st...
journal_title:Current stem cell research & therapy
pub_type: 杂志文章
doi:10.2174/1574888X15999200831160710
更新日期:2020-08-31 00:00:00
abstract:BACKGROUND:The urinary bladder and urethra comprise the lower urinary tracts. Pathological conditions that affect both structures necessitate reconstructive urological intervention with autologous tissue sources that cause neuromechanical and metabolic complications. Stem-cell therapies may offer an attractive alternat...
journal_title:Current stem cell research & therapy
pub_type: 杂志文章,评审
doi:10.2174/1574888X13666180426113907
更新日期:2018-01-01 00:00:00
abstract::Differentiation of stem cells, a crucial step in the process of tissue development, repair and regeneration, can be regulated by a variety of mechanical factors such as the stiffness of extracellular matrix. In this review article, the effects of stiffness on the differentiation of stem cells, including bone marrow-de...
journal_title:Current stem cell research & therapy
pub_type: 杂志文章
doi:10.2174/1574888X15666200408114632
更新日期:2020-01-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) have been isolated not only from bone marrow, but also from many other tissues such as adipose tissue, skeletal muscle, liver, brain and pancreas. Because MSC were found to have the ability to differentiate into cells of multiple organs and systems such as bone, fat, cartilage, muscle, ne...
journal_title:Current stem cell research & therapy
pub_type: 杂志文章,评审
doi:10.2174/157488806778226821
更新日期:2006-09-01 00:00:00
abstract::The liver has a remarkable capability to restore its functional capacity following liver injury. According to the current paradigm, differentiated and usually quiescent hepatocytes are the primary cell type responsible for liver repair. As reserve compartment, bipotent hepatic progenitor cells are activated, especiall...
journal_title:Current stem cell research & therapy
pub_type: 杂志文章,评审
doi:10.2174/157488807779317062
更新日期:2007-01-01 00:00:00
abstract::Chronic myeloproliferative disorders (cMPDs) are clonal hemopoietic malignancies arising at the multipotent stem cell level. These conditions are characterized by increased blood count, marrow hyperplasia and extramedulary hemopoiesis. Vascular events might complicate their course, and transformation to either acute l...
journal_title:Current stem cell research & therapy
pub_type: 杂志文章,评审
doi:10.2174/157488809788167445
更新日期:2009-05-01 00:00:00
abstract::Mesenchymal stem cells (MSC) can be obtained from human bone marrow aspirates and, thanks to their differentiation potential and excellent in vitro culture properties, represent an attractive cell line for the regeneration of mesenchymal tissue. Both in vitro and in vivo, they can differentiate into cartilage, bone, t...
journal_title:Current stem cell research & therapy
pub_type: 杂志文章,评审
doi:10.2174/157488806775269151
更新日期:2006-01-01 00:00:00
abstract::Recent studies have shown that treatments involving injection of stem cells into animals with damaged cardiac tissue result in improved cardiac functionality. Clinical trials have reported conflicting results concerning the recellularization of post-infarct collagen scars. No clear mechanism has so far emerged to full...
journal_title:Current stem cell research & therapy
pub_type: 杂志文章,评审
doi:10.2174/157488812798483449
更新日期:2012-01-01 00:00:00
abstract::Due to the advent of less-toxic reduced intensity conditioning regimens (RIT), allogeneic hematopoietic cell transplantation (allo-HCT) is currently widely used to treat chemotherapy-resistant hematological diseases, particularly in older patients,which are the fastest growing group of patients receiving allo-HCT. How...
journal_title:Current stem cell research & therapy
pub_type: 杂志文章,评审
doi:10.2174/157488812799859856
更新日期:2012-05-01 00:00:00