Abstract：
OBJECTIVE:The aim of this study was to investigate the expression level and potential molecular mechanism of microRNA-1324 in gastric cancer (GCa) to provide a new perspective for the diagnosis and treatment of GCa. PATIENTS AND METHODS:The expression levels of microRNA-1324 and MECP2 in GCa tissues and cell lines were detected using quantitative Real Time Polymerase Chain Reaction (qRT-PCR). The influence of microRNA-1324 and MECP2 on the proliferation or invasiveness of GCa cells were investigated by cell counting kit-8 (CCK-8) and colony formation assay or transwell assay, respectively. Furthermore, the regulatory interplay between microRNA-1324 and MECP2 was verified via Dual-Luciferase reporting assay, qRT-PCR, and Western Blot. RESULTS:QRT-PCR results revealed that microRNA-1324 expression was remarkably down-regulated in GCa tissues and cell lines, while the expression of MECP2 was remarkably up-regulated. Subsequently, we confirmed that miR-1324 could target and bind to MECP2, as well as inhibit its expression. Inhibition of microRNA-1324 remarkably enhanced the proliferative capacity and invasiveness of GCa cells. However, opposite results were observed after inhibiting MECP2 expression. At the same time, flow cytometry revealed that inhibition of microRNA-1324 accelerated cell cycle but inhibited apoptosis. Conversely, opposite results were observed when MECP2 was down-regulated in vitro. CONCLUSIONS:MicroRNA-1324 was remarkably down-regulated in GCa tissues or cell lines. Meanwhile, it could inhibit MECP2 expression, and promote the proliferation and invasion of GCa cells, eventually participating in the occurrence and development of GCa.
journal_name
Eur Rev Med Pharmacol Scijournal_title
European review for medical and pharmacological sciencesauthors
Zhang XY,Xu YY,Chen WYdoi
10.26355/eurrev_202005_21165subject
Has Abstractpub_date
2020-05-01 00:00:00pages
4766-4774issue
9eissn
1128-3602issn
2284-0729journal_volume
24pub_type
杂志文章abstract：INTRODUCTION:To investigate the relationship between the interleukin-10 (IL-10) gene polymorphism and the pathogenesis of diabetic retinopathy (DR). PATIENTS AND METHODS:According to the fundus examination report, 420 patients with diabetes were divided into the non-DR group (n=200) and the DR group (n=220). The singl...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201906_18169
更新日期：2019-06-01 00:00:00
abstract：OBJECTIVES:Our aim was to investigate whether the maternal serum concentrations of first and second trimester serum analytes are altered in familial Mediterranean fever (FMF) pregnancies. MATERIALS AND METHODS:The screening tests were compared in a series of 16 serum samples from FMF pregnancies and in a cohort of 48 ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2013-07-01 00:00:00
abstract：OBJECTIVE:To explore the influence and the underlying mechanism of micro-ribonucleic acid (miR)-34a on cerebral neuronal apoptosis in rats with cerebral ischemia-reperfusion (CIR). MATERIALS AND METHODS:60 adult male Wistar rats were randomly divided into 3 groups: Sham group, CIR group and miR-34a knockdown group. Th...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201909_19021
更新日期：2019-09-01 00:00:00
abstract：OBJECTIVE:To evaluate the recurrent ischemic events and risk factors in patients with symptomatic intracranial artery stenosis. PATIENTS AND METHODS:Patients with acute cerebral infarction or transient ischemia attach (TIA) and intracranial arterial stenosis confirmed through CTA examination, were enrolled from the De...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2015-01-01 00:00:00
abstract：OBJECTIVE:In the clinic, therapeutic options for pulmonary arterial hypertension are limited; therefore, investigating the therapeutic strategies and novel therapies is critical for pulmonary arterial hypertension (PAH) treatment. This study aimed to evaluate the role of miRNA-126 (miR-126) and its associated signaling...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201904_17588
更新日期：2019-04-01 00:00:00
abstract：OBJECTIVE:The purpose of this study was to elucidate the possible beneficial effects of AcF on acute lung injury (ALI) in a rat model of sepsis. MATERIALS AND METHODS:Male Sprague-Dawley rats were randomly divided into the following four experimental groups (n = 10 per group): animals undergoing a sham cecal ligature ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：:This review illustrates some brief considerations of the medical use of cannabis recently issued in Italy. History and uses of cannabis throughout centuries and different countries are illustrated together with a description of botany and active phytocannabinoids. Then, medical use of cannabis anti-pain treatment for ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,评审
doi：10.26355/eurrev_201802_14405
更新日期：2018-02-01 00:00:00
abstract：OBJECTIVE:To investigate the expression of miR-182 in patients with fracture of tibial plateau (FTP) and its effects on osteoblasts and fracture healing. PATIENTS AND METHODS:The patients with fracture of tibial plateau treated in our hospital and healthy subjects who received physical examination from January 2017 to...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_202101_24343
更新日期：2021-01-01 00:00:00
abstract：OBJECTIVE:The objective of this case report is to describe the management of anesthesia of a patient with an LVAD that underwent an emergency open appendectomy. Literature regarding emergency anesthesia management of such patients is still limited. A search in the PubMed engine with the keywords "LVAD appendectomy anes...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2016-08-01 00:00:00
abstract：BACKGROUND:Osteosarcoma is an aggressive cancerous neoplasm arising from primitive transformed cells of mesenchymal origin that exhibit osteoblastic differentiation and produce malignant osteoid. With the rapid development of tumor molecular biology, gene and viral therapy, a highly promising strategy for the treatment...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2013-03-01 00:00:00
abstract：OBJECTIVES:Aggressive cell growth and chemoresistance are notorious obstacles in neuroblastoma therapy. Accumulating evidence suggests that survivin is preferentially expressed in cancer cells and plays a crucial role in cell division and apoptosis dysfunction. Thus, in the present study, we investigated whether silenc...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2013-11-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to explore the regulatory mechanism of micro ribonucleic acid (miR)-223 in ulcerative colitis (UC) through the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt)-mammalian target of rapamycin (mTOR) signaling pathway. MATERIALS AND METHODS:A total of 36 Sprague-Dawley (SD) ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201906_18074
更新日期：2019-06-01 00:00:00
abstract：OBJECTIVE:To investigate the effectiveness of tranexamic acid (TA) application in two techniques: in the first one wound closure is performed before the tourniquet is released; in the second one, wound closure is performed after the tourniquet is released. PATIENTS AND METHODS:The study is conducted on four groups of ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,随机对照试验
doi：10.26355/eurrev_201809_15952
更新日期：2018-09-01 00:00:00
abstract：OBJECTIVE:Colonoscopy is recognized as the primary screening test for colorectal cancer. However, its inaccuracy in identifying the exact tumor localization is still high. As a consequence, repeated colonoscopies and changes in the surgical management have been reported. This study aims to evaluate the quality of 216 c...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201903_17401
更新日期：2019-03-01 00:00:00
abstract：OBJECTIVE:To elucidate the function of long non-coding RNA (lncRNA) SNHG1 in acute myeloid leukemia (AML) and to explore its biological mechanism. PATIENTS AND METHODS:Expression levels of lncRNA SNHG1 and miR-488-5p in AML cell lines pAML and THP-1 were determined by quantitative Real Time-Polymerase Chain Reaction (...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201907_18334
更新日期：2019-07-01 00:00:00
abstract：OBJECTIVE:CircRNAs serve an essential role in regulating the development and progression of various tumors. The aim of this study was to examine the role and mechanism of circ_0009910 in hepatocellular carcinoma (HCC). MATERIALS AND METHODS:RT-qPCR was used to detect the expression of circ_0009910 and miR-335-5p in ti...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_202002_20349
更新日期：2020-02-01 00:00:00
abstract：:Tamoxifen is a synthetic non steroidal anti-estrogen used to treat patients with breast cancer and healthy subjects with high risk of breast cancer. It was aimed to study the short term effects of tamoxifen on the plasma total antioxidant capacity (TAC), nitric oxide (NO) and the adenosine deaminase activity (ADA) in ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2009-07-01 00:00:00
abstract：:The prevalence of asthma and allergic diseases in childhood has increased in several industrialized countries since the second half of the twentieth century. In some countries, the prevalence is still rising, although in others it seems to have plateaued or even decreased. It has been suggested that environmental fact...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,收录出版,评审
doi：
更新日期：2016-01-01 00:00:00
abstract：OBJECTIVE:To study the application value of artificial skin substitute compositely constructed by adipose-derived stem cells and fibrin gel in skin defect. MATERIALS AND METHODS:Adipose-derived stem cells (ADSCs) were obtained from healthy pure green fluorescent protein (GFP) transgenic mice and were proved to have mu...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2017-07-01 00:00:00
abstract：OBJECTIVE:Sepsis refers to the systemic inflammatory response caused by infection. Acute kidney injury (AKI) in sepsis is very common, and there are many complicated mechanisms for the occurrence of septic AKI. This article aimed to study the role of miR-942-5p in inflammation and apoptosis of septic AKI and its potent...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_202006_21521
更新日期：2020-06-01 00:00:00
abstract：OBJECTIVE:To investigate the therapeutic effect of drug therapy with cyclophosphamide and leflunomide on the joint function damage of patients with systemic lupus erythematosus (SLE) and its regulatory effects on expression levels of programmed death receptor 1, Notch signaling pathway genes and interferon-inducible pr...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章,随机对照试验
doi：
更新日期：2017-10-01 00:00:00
abstract：OBJECTIVE:Our study aims to demonstrate that the use in the preconceptional period until the 24th week of pregnancy of inositol and folic acid, first of all, preserves the product of conception from neural tube defects (NTDs) and then, thanks to inositol supplementation, it possibly counteracts and prevents the onset o...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2017-07-01 00:00:00
abstract：OBJECTIVE:Prevalence of asthma and vitamin D deficiency has been increasing and leading to significant morbidities. This study aimed to compare the vitamin D levels in the pre-school children with asthma and in healthy controls and to assess the relationship between vitamin D levels and asthma clinical parameters and c...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2016-01-01 00:00:00
abstract：OBJECTIVE:The purpose of this study was to elucidate the regulatory effects of microRNA-655-3p (miR-655-3p) on growth and invasiveness of trophoblasts to influence the development of preeclampsia (PE). PATIENTS AND METHODS:Relative levels of miR-655-3p and PBX3 in 24 PE pregnant women and 24 healthy ones were examined...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_202010_23383
更新日期：2020-10-01 00:00:00
abstract：BACKGROUND AND OBJECTIVES:Leishmania parasites are intracellular haemoflagellates that infect macrophages of the skin and viscera to produce diseases in their vertebrates hosts. Antileishmania therapy is based on pentavalent antimony compounds which toxicity of these agents and the persistence of side effects are sever...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2013-12-01 00:00:00
abstract：OBJECTIVE:To determine the mRNA and protein expression, and methylation levels of the Klotho in lens epithelial cells (LECs) of normal transparent lenses and age-related cataracts (ARCs), and to explore the role of epigenetic changes of the Klotho gene in regulating the development of ARCs. PATIENTS AND METHODS:A tota...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2015-01-01 00:00:00
abstract：OBJECTIVE:This study aims to explore whether ZEB2-AS1 can promote the development of osteosarcoma by affecting the proliferation, invasion, and apoptosis of osteosarcoma cells. PATIENTS AND METHODS:Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was performed to detect the ZEB2-AS1 expression in osteosarcom...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_202006_21637
更新日期：2020-06-01 00:00:00
abstract：OBJECTIVE:Menin, encoded by the Men1 gene, is responsible for β-cell tumor formation in patients with multiple endocrine neoplasia type 1. Recently, Menin has been proven to negatively regulate β-cell proliferation in several mouse models, including hyperglycemia. However, it is unclear how glucose regulates Menin expr...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：
更新日期：2015-02-01 00:00:00
abstract：OBJECTIVE:This study aimed to investigate the expression of TRPM7 in the serum of patients with sepsis and its influences on inflammatory factors and prognosis. PATIENTS AND METHODS:A prospective analysis was performed. 78 patients with sepsis were enrolled in the experimental group and treated from May 2015 to April ...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201905_17821
更新日期：2019-05-01 00:00:00
abstract：OBJECTIVE:Primary liver cancer is a sort of the most common solid tumors occurred in the digestive system. The incidence and mortality rate maintain at a high level, thus leading to heavy economic and psychological burdens for patients. Next-generation biological therapy, such as cellular immune treatment, improves the...
journal_title：European review for medical and pharmacological sciences
pub_type： 杂志文章
doi：10.26355/eurrev_201802_14312
更新日期：2018-02-01 00:00:00
