Influence of miR-34a on cerebral neuronal apoptosis in rats with cerebral ischemia reperfusion through the Notch1 signaling pathway.

Abstract:

OBJECTIVE:To explore the influence and the underlying mechanism of micro-ribonucleic acid (miR)-34a on cerebral neuronal apoptosis in rats with cerebral ischemia-reperfusion (CIR). MATERIALS AND METHODS:60 adult male Wistar rats were randomly divided into 3 groups: Sham group, CIR group and miR-34a knockdown group. The rat model of CIR was established using the suture occlusion method. The expression level of miR-34a in lesion tissues in the three groups was determined via Reverse Transcription-Polymerase Chain Reaction (RT-PCR). The pathological injury of brain tissues was detected via hematoxylin-eosin (HE) staining and the infarction region in each group was evaluated via triphenyl tetrazolium chloride (TTC) staining. The terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining was performed to measure the cerebral neuronal apoptosis level. The level of Nissl's body in each group was detected via Nissl's staining. The expression level of the platelet-derived neurotrophic factor (PDNF) and Notch1/hypoxia-inducible factor-1α (HIF-1α) signaling pathway-related proteins in brain tissues were detected via immunohistochemistry and Western blotting, respectively. RESULTS:The expression level of miR-34a in brain tissues of the CIR group was significantly increased compared to that of the Sham group (p < 0.05). After the intervention with miR-34a, the infarction area of brain tissues was markedly reduced when comparing to the CIR group (p < 0.05). In addition, the results of HE staining and Nissl staining revealed that CIR treatment led to edema in cerebral neurons, disorderly arranged neurons, and remarkably decreased number of Nissl's bodies. However, miR-34a knockdown following CIR significantly alleviated the brain tissue injury and markedly increased the number of Nissl's bodies (p < 0.05). The results of TUNEL staining also indicated that miR-34a siRNA could remarkably reverse the cerebral neuronal apoptosis caused by CIR in rats (p < 0.05). According to the immunohistochemical staining results, the expression of PDNF in brain tissues declined in the CIR group, while enhanced in the miR-34a siRNA group (p < 0.05). Furthermore, the Western blotting results manifested that miR-34a siRNA could up-regulate the Notch1 and HIF-1α protein expressions in brain tissues of CIR rats. CONCLUSIONS:Our data demonstrated that the miR-34a knockdown could alleviate the brain tissue injury and neuronal apoptosis by activating the Notch1/HIF-1α signaling pathway CIR-treated rats.

authors

Wang SP,Wang D,Li HX,Liu L,Duan XH

doi

10.26355/eurrev_201909_19021

subject

Has Abstract

pub_date

2019-09-01 00:00:00

pages

8049-8057

issue

18

eissn

1128-3602

issn

2284-0729

journal_volume

23

pub_type

杂志文章
  • Efficacy of botulinum toxin A for treatment of unilateral spasms of the eyelid and its prognosis.

    abstract:BACKGROUND:To this date, only a small number of studies have described the long-term use of BTX in Hemifacial spasm. AIM:To assess the prognosis and long-term effectiveness of Botulinum toxin A (BTXA) in the treatment of unilateral spasms of the eyelid. PATIENTS AND METHODS:From September 1998 to January 2006, 245 co...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:

    authors: Xu YP,Shen J,Zhu QB,Gu J,Lin SZ,Fan JP

    更新日期:2013-11-01 00:00:00

  • Promotion of proliferation and metastasis of hepatocellular carcinoma by LncRNA00673 based on the targeted-regulation of notch signaling pathway.

    abstract:OBJECTIVE:To investigate the relative expression of long non-coding RNA 00673 (lncRNA 00673) in hepatocellular carcinoma (HCC) and HCC cells and study its regulation on the malignant phenotype of HCC cells PATIENTS AND METHODS: Samples of HCC and adjacent tissues from January 2013 to December 2015 were collected. The e...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:

    authors: Chen H,Liu JZ,Hu GJ,Shi LL,Lan T

    更新日期:2017-08-01 00:00:00

  • MiR-506 alleviates myocardial ischemia-reperfusion injury via targeting PI3K/AKT.

    abstract:OBJECTIVE:The aim of this study was to explore the effects of micro ribonucleic acid (miR)-506 and phosphatidylinositol 3-hydroxy kinase (PI3K)/protein kinase B (AKT) pathway on myocardial ischemia-reperfusion injury (MIRI) in rats. MATERIALS AND METHODS:A total of 90 healthy rats weighing 260-300 g were selected as r...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_202012_24193

    authors: Zhang M,Wang JY,Li L,Li GM

    更新日期:2020-12-01 00:00:00

  • Coronavirus disease 2019: a bibliometric analysis and review.

    abstract:OBJECTIVE:On December 8, 2019, many cases of pneumonia with unknown etiology were first reported in Wuhan, China, subsequently identified as a novel coronavirus infection aroused worldwide concern. As the outbreak is ongoing, more and more researchers focused interest on the COVID-19. Therefore, we retrospectively anal...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_202003_20712

    authors: Lou J,Tian SJ,Niu SM,Kang XQ,Lian HX,Zhang LX,Zhang JJ

    更新日期:2020-03-01 00:00:00

  • Diverse effects of taurine on vascular response and inflammation in GSH depletion model in rabbits.

    abstract:OBJECTIVE:A reduction in GSH and an increase in free radicals are observed in inflammatory diseases, indicating oxidative stress. Taurine protects cells from the cytotoxic effects of inflammation. There have been limited studies to date evaluating the effect of taurine in oxidative stress-induced vascular dysfunction a...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:

    authors: Ozsarlak-Sozer G,Sevin G,Ozgur HH,Yetik-Anacak G,Kerry Z

    更新日期:2016-04-01 00:00:00

  • Long non-coding RNA CRNDE is a novel tumor promoter by modulating PI3K/AKT signal pathways in human gastric cancer.

    abstract:OBJECTIVE:Long non-coding RNA CRNDE (CRNDE) recently emerged as a carcinogenic promoter in various cancers including gastric cancer (GC). However, the functions and molecular mechanisms of CRNDE to GC are still largely unclear. The aim of this study was to investigate the clinical significance and functional mechanisms...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_201712_13925

    authors: Du DX,Lian DB,Amin BH,Yan W

    更新日期:2017-12-01 00:00:00

  • Folic acid attenuates dexamethasone-induced placental growth restriction.

    abstract:OBJECTIVE:Intrauterine glucocorticoid (GC) exposure is associated with disturbances in feto-placental growth. This study aimed to investigate whether folic acid supplementation can prevent dexamethasone (Dex)-induced feto-placental growth restriction. MATERIALS AND METHODS:Female C57BL/6J mice were subject to four dif...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:

    authors: Zhang A,Zhou LF,Xiang XL,Wang K,Zhou Q,Duan T

    更新日期:2015-04-01 00:00:00

  • Human parvovirus B 19 and Epstein-Barr virus co-infection in a child with hereditary spherocytosis.

    abstract:BACKGROUND:In patients with chronic congenital haemolytic disorders, human Parvovirus B19 (HPV B19) is frequently involved in pure red-cell aplastic crises. Furthermore, it may inhibit three-lineage haematopoiesis in the bone marrow, causing severe pancytopenia. In such patients, Epstein Barr virus (EBV) infection also...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:

    authors: Cefalo MG,Arlotta A,Maurizi P,Russo I,Sani I,Battista A,Mastrangelo S,Ruggiero A,Riccardi R

    更新日期:2012-02-01 00:00:00

  • 2-Aminoethoxydiphenyl borate reduces degranulation and release of cytokines in a rat mast cell line.

    abstract:BACKGROUND AND OBJECTIVE:2-aminoethoxydiphenyl borate (2-APB) has been reported to be a useful pharmacological tool in the study of calcium signaling. It is used either as a membrane-permeable inhibitor for inositol 1,4,5-trisphosphate (IP3) receptors or a store-operated calcium (Ca2+) entry (SOCE) inhibitor. The prese...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:

    authors: Ng NM,Jiang SP,Zhang W

    更新日期:2012-08-01 00:00:00

  • Medical use of cannabis: Italian and European legislation.

    abstract::This review illustrates some brief considerations of the medical use of cannabis recently issued in Italy. History and uses of cannabis throughout centuries and different countries are illustrated together with a description of botany and active phytocannabinoids. Then, medical use of cannabis anti-pain treatment for ...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章,评审

    doi:10.26355/eurrev_201802_14405

    authors: Zaami S,Di Luca A,Di Luca NM,Montanari Vergallo G

    更新日期:2018-02-01 00:00:00

  • Effect of single nucleotide polymorphisms on CDK4 and Zn supplementation in children with growth hormone deficiency.

    abstract:OBJECTIVE:Precision medicine is a personalized disease prevention and treatment program combining modern genetic technology, molecular imaging techniques, and biological information with patients' living environment and clinical data, for accurate classification and diagnosis of diseases. CASE REPORT:Our study present...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:

    authors: Liu Y,Zhang ZX

    更新日期:2016-10-01 00:00:00

  • Cow milk induced allergies (CMA) and asthma in new born.

    abstract::The prevalence of asthma and allergic diseases in childhood has increased in several industrialized countries since the second half of the twentieth century. In some countries, the prevalence is still rising, although in others it seems to have plateaued or even decreased. It has been suggested that environmental fact...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章,收录出版,评审

    doi:

    authors: Wang W,Wu HW,Liu JF

    更新日期:2016-01-01 00:00:00

  • The role of cefditoren in the treatment of lower community-acquired respiratory tract infections (LRTIs): from bacterial eradication to reduced lung inflammation and epithelial damage.

    abstract:OBJECTIVES:Lower respiratory tract infections (LRTIs), including pneumonia and acute exacerbations of Chronic Obstructive Pulmonary Disease (COPD), are among the most common diagnoses in both outpatient and inpatient settings. Due to the burden of LRTIs healthcare providers must adopt practices focused on improving out...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章,评审

    doi:

    authors: Di Marco F,Braido F,Santus P,Scichilone N,Blasi F

    更新日期:2014-01-01 00:00:00

  • Comparison of imaging features and diagnostic values of MRI, CT and contrast-enhanced ultrasonography in the diagnosis of cervical carcinoma staging.

    abstract:OBJECTIVE:This study aimed at exploring and contrasting the clinical significances and values of MRI, CT and contrast-enhanced ultrasonography in FIGO staging of cervical carcinoma. PATIENTS AND METHODS:The contrast-enhanced ultrasonography, CT and MRI imaging data of 348 patients with cervical carcinoma confirmed by ...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_201808_15612

    authors: Yu L,Zhang HF,Jiang DW,Zhao DY,Liu H,Shen LM

    更新日期:2018-08-01 00:00:00

  • Nitric oxide mediated effects of nebivolol in myocardial infarction: the source of nitric oxide.

    abstract:OBJECTIVE:After MI pathological LV remodeling is one of the major causes of death. We previously showed the NO mediated beneficial effects of nebivolol in rat MI model, in this study we aimed to evaluate the NOS related mechanisms in this phenomenon. MATERIALS AND METHODS:Rats were divided into four groups: sham opera...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:

    authors: Mercanoglu G,Safran N,Ahishali B B,Uzun H,Yalcin A,Mercanoglu F

    更新日期:2015-12-01 00:00:00

  • MicroRNA-325 alleviates myocardial fibrosis after myocardial infarction via downregulating GLI1.

    abstract:OBJECTIVE:To explore the role of microRNA-326 in myocardial fibrosis after myocardial infarction (MI) and its underlying mechanism. MATERIALS AND METHODS:MI rat model was constructed via left anterior descending artery (LAD) ligation. Infarct tissues, infarct border zone tissues and remote zone tissues were harvested ...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_201808_15735

    authors: Wang CC,Shang BB,Yang CW,Liu YF,Li XD,Wang SY

    更新日期:2018-08-01 00:00:00

  • Predictors of outcome for patients with severe respiratory failure requiring non invasive mechanical ventilation.

    abstract:OBJECTIVE:Failure to identify patients in whom non-invasive ventilation (NIV) would be unsuccessful may cause inappropriate delay in intubation. Aim of this study was to determine predictors of NIV failure. PATIENTS AND METHODS:We retrospectively evaluated COPD patients, undergoing NIV for at least 12h because of hype...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:

    authors: Conti V,Paone G,Mollica C,Sebastiani A,Mannocci A,La Torre G,Cardaci V,Cammarella I,Puglisi G,Brunetti G,Alma MG,Terzano C

    更新日期:2015-10-01 00:00:00

  • Clinical application of remifentanil in local anesthesia for tumor resection in functional brain area.

    abstract:OBJECTIVE:The aim of this study was to investigate clinical application of remifentanil in local anesthesia for resection of tumors in functional brain area. PATIENTS AND METHODS:Twenty-four patients were randomly divided into two groups: control group and remifentanil group. In remifentanil group remifentanil was inf...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章,随机对照试验

    doi:

    authors: Cao SE,Gao BQ,Zhang YQ,Yang SM,Liu GZ,Zhang XR

    更新日期:2014-01-01 00:00:00

  • An update on the status of COVID-19: a comprehensive review.

    abstract::The last two decades have witnessed two large-scale pandemics caused by coronaviruses, including severe acute respiratory syndrome (SARS) and the Middle East respiratory syndrome (MERS). At the end of 2019, another novel coronavirus, designated as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), hit Wuhan...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章,评审

    doi:10.26355/eurrev_202004_21046

    authors: Zhao N,Zhou ZL,Wu L,Zhang XD,Han SB,Bao HJ,Shu Y,Shu XG

    更新日期:2020-04-01 00:00:00

  • Long non-coding RNA (lncRNA) small nucleolar RNA host gene 7 (SNHG7) promotes breast cancer progression by sponging miRNA-381.

    abstract:OBJECTIVE:The abnormal expression of lncRNA small nucleolar RNA host gene 7 (SNHG7) has been found in various kinds of cancers but its expression and role in breast cancer are unknown. We conducted this study to identify the expression, the function, and to underline the mechanism of SNHG7 in breast cancer. PATIENTS A...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_201908_18545

    authors: Gao YT,Zhou YC

    更新日期:2019-08-01 00:00:00

  • Induction of esophageal cancers by nitenpyram (NIT) in rats.

    abstract:OBJECTIVE:This study was made to investigate and evaluate the safety and carcinogenicity of nitenpyram (NIT) in rats. MATERIALS AND METHODS:A totally 50 male and 50 female SD rats were treated with NIT at 0, 800, 2400, and 7200 ppm, respectively, for 104 w. The growth, clinical signs, and survival rates, as well as th...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_201812_16635

    authors: Xing LG,Wu YL,Zheng HN,Jia YY,Wu GF,Yang C

    更新日期:2018-12-01 00:00:00

  • The role of miR-146b-5p in TLR4 pathway of glomerular mesangial cells with lupus nephritis.

    abstract:OBJECTIVE:MicroRNAs (miRNAs) are now recognized as important regulators of gene expression. The aim of the study was to investigate the role of miR-146b-5p in the TLR4 pathway and provide the basis for the treatment of lupus nephritis. MATERIALS AND METHODS:The glomerular mesangial cells were cultured in vitro and div...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_201803_14589

    authors: Sheng ZX,Yao H,Cai ZY

    更新日期:2018-03-01 00:00:00

  • Evaluation of in vitro antileishmanial activity of curcumin and its derivatives "gallium curcumin, indium curcumin and diacethyle curcumin".

    abstract:BACKGROUND AND OBJECTIVES:Leishmania parasites are intracellular haemoflagellates that infect macrophages of the skin and viscera to produce diseases in their vertebrates hosts. Antileishmania therapy is based on pentavalent antimony compounds which toxicity of these agents and the persistence of side effects are sever...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:

    authors: Fouladvand M,Barazesh A,Tahmasebi R

    更新日期:2013-12-01 00:00:00

  • Long noncoding RNA LUCAT1 promotes migration and invasion of prostate cancer cells by inhibiting KISS1 expression.

    abstract:OBJECTIVE:Recent researches have revealed the role of long noncoding RNAs (lncRNAs) in tumor development. In this study, the potential function of lncRNA LUCAT1 in the progression of prostate cancer was identified. PATIENTS AND METHODS:Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) was used to detect LUCAT...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_201904_17689

    authors: Liu C,Wang L,Li YW,Cui YS,Wang YQ,Liu S

    更新日期:2019-04-01 00:00:00

  • MiR-152-3p promotes the development of chronic myeloid leukemia by inhibiting p27.

    abstract:OBJECTIVE:The aim of this study was to investigate the relationship between micro ribonucleic acid (miR)-152-3p and chronic myeloid leukemia (CML), and to explore the underlying mechanism. PATIENTS AND METHODS:The expression level of miR-152-3p in the bone marrow of 40 CML patients and 30 normal controls was detected ...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_201812_16646

    authors: Wang L,Wang Y,Lin J

    更新日期:2018-12-01 00:00:00

  • Butorphanol protects on myocardial ischemia/reperfusion injury in rats through MAPK signaling pathway.

    abstract:OBJECTIVE:To explore the influence of butorphanol on myocardial ischemia/reperfusion (I/R) injury in rats through the mitogen-activated protein kinase (MAPK) signaling pathway. MATERIALS AND METHODS:The I/R model in Sprague-Dawley rats was established. The rats were randomly divided into normal group (n=20), myocardia...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_201912_19695

    authors: Wang H,Wang JL,Ren HW,He WF,Sun M

    更新日期:2019-12-01 00:00:00

  • The relationship between intracranial pressure and neurocognitive function before and after the repair of a skull injury.

    abstract:OBJECTIVE:This study examines the relationship between intracranial pressure (ICP) changes after skull injury repair and neurocognitive function before and after the repair. PATIENTS AND METHODS:Sixty patients undergoing skull injury repair participated in the study. A non-invasive detection analyzer was used to detec...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:

    authors: He J,Chen LL,Sun DK,Wang HT,Wang JJ,Zhai X

    更新日期:2017-03-01 00:00:00

  • A novel homozygous TPM1 mutation in familial pediatric hypertrophic cardiomyopathy and in silico screening of potential targeting drugs.

    abstract:OBJECTIVE:Familial hypertrophic cardiomyopathy (HCM) is the most common genetic cardiac disease. While sarcomeric gene mutations explain many HCM cases, the genetic basis of about half of HCM cases remains elusive. Here we aimed to identify the gene causing HCM in a non-consanguineous Saudi Arabian family with affected...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_202007_22299

    authors: Carlus SJ,Almuzaini IS,Karthikeyan M,Loganathan L,Al-Harbi GS,Carlus FH,Al-Mazroea AH,Morsy MM,Abo-Haded HM,Abdallah AM,Al-Harbi KM

    更新日期:2020-07-01 00:00:00

  • HDAC1 is indirectly involved in the epigenetic regulation of p38 MAPK that drive the lung cancer progression.

    abstract:OBJECTIVE:p38 MAPK are a class of protein kinase that may induce or prevent apoptosis in different circumstance. Emerging researches show that it plays a vital role in tumor progression and therefore understanding its dual role in different stages of lung cancer are important to investigate. Also in this study, we plan...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 杂志文章

    doi:10.26355/eurrev_201809_15932

    authors: Dong ZY,Zhou YR,Wang LX

    更新日期:2018-09-01 00:00:00

  • Inositol: history of an effective therapy for Polycystic Ovary Syndrome.

    abstract::Inositol is a physiological compound belonging to the sugar family. The two inositol stereoisomers, myo-inositol and D-chiroinositol are the two main stereisomers present in our body. Myo-inositol is the precursor of inositol triphosphate, a second messenger regulating many hormones such as TSH, FSH and insulin. D-chi...

    journal_title:European review for medical and pharmacological sciences

    pub_type: 历史文章,杂志文章

    doi:

    authors: Bizzarri M,Carlomagno G

    更新日期:2014-07-01 00:00:00