Abstract:
OBJECTIVE:To explore the influence and the underlying mechanism of micro-ribonucleic acid (miR)-34a on cerebral neuronal apoptosis in rats with cerebral ischemia-reperfusion (CIR). MATERIALS AND METHODS:60 adult male Wistar rats were randomly divided into 3 groups: Sham group, CIR group and miR-34a knockdown group. The rat model of CIR was established using the suture occlusion method. The expression level of miR-34a in lesion tissues in the three groups was determined via Reverse Transcription-Polymerase Chain Reaction (RT-PCR). The pathological injury of brain tissues was detected via hematoxylin-eosin (HE) staining and the infarction region in each group was evaluated via triphenyl tetrazolium chloride (TTC) staining. The terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining was performed to measure the cerebral neuronal apoptosis level. The level of Nissl's body in each group was detected via Nissl's staining. The expression level of the platelet-derived neurotrophic factor (PDNF) and Notch1/hypoxia-inducible factor-1α (HIF-1α) signaling pathway-related proteins in brain tissues were detected via immunohistochemistry and Western blotting, respectively. RESULTS:The expression level of miR-34a in brain tissues of the CIR group was significantly increased compared to that of the Sham group (p < 0.05). After the intervention with miR-34a, the infarction area of brain tissues was markedly reduced when comparing to the CIR group (p < 0.05). In addition, the results of HE staining and Nissl staining revealed that CIR treatment led to edema in cerebral neurons, disorderly arranged neurons, and remarkably decreased number of Nissl's bodies. However, miR-34a knockdown following CIR significantly alleviated the brain tissue injury and markedly increased the number of Nissl's bodies (p < 0.05). The results of TUNEL staining also indicated that miR-34a siRNA could remarkably reverse the cerebral neuronal apoptosis caused by CIR in rats (p < 0.05). According to the immunohistochemical staining results, the expression of PDNF in brain tissues declined in the CIR group, while enhanced in the miR-34a siRNA group (p < 0.05). Furthermore, the Western blotting results manifested that miR-34a siRNA could up-regulate the Notch1 and HIF-1α protein expressions in brain tissues of CIR rats. CONCLUSIONS:Our data demonstrated that the miR-34a knockdown could alleviate the brain tissue injury and neuronal apoptosis by activating the Notch1/HIF-1α signaling pathway CIR-treated rats.
journal_name
Eur Rev Med Pharmacol Scijournal_title
European review for medical and pharmacological sciencesauthors
Wang SP,Wang D,Li HX,Liu L,Duan XHdoi
10.26355/eurrev_201909_19021subject
Has Abstractpub_date
2019-09-01 00:00:00pages
8049-8057issue
18eissn
1128-3602issn
2284-0729journal_volume
23pub_type
杂志文章abstract:BACKGROUND:To this date, only a small number of studies have described the long-term use of BTX in Hemifacial spasm. AIM:To assess the prognosis and long-term effectiveness of Botulinum toxin A (BTXA) in the treatment of unilateral spasms of the eyelid. PATIENTS AND METHODS:From September 1998 to January 2006, 245 co...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2013-11-01 00:00:00
abstract:OBJECTIVE:To investigate the relative expression of long non-coding RNA 00673 (lncRNA 00673) in hepatocellular carcinoma (HCC) and HCC cells and study its regulation on the malignant phenotype of HCC cells PATIENTS AND METHODS: Samples of HCC and adjacent tissues from January 2013 to December 2015 were collected. The e...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2017-08-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to explore the effects of micro ribonucleic acid (miR)-506 and phosphatidylinositol 3-hydroxy kinase (PI3K)/protein kinase B (AKT) pathway on myocardial ischemia-reperfusion injury (MIRI) in rats. MATERIALS AND METHODS:A total of 90 healthy rats weighing 260-300 g were selected as r...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202012_24193
更新日期:2020-12-01 00:00:00
abstract:OBJECTIVE:On December 8, 2019, many cases of pneumonia with unknown etiology were first reported in Wuhan, China, subsequently identified as a novel coronavirus infection aroused worldwide concern. As the outbreak is ongoing, more and more researchers focused interest on the COVID-19. Therefore, we retrospectively anal...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202003_20712
更新日期:2020-03-01 00:00:00
abstract:OBJECTIVE:A reduction in GSH and an increase in free radicals are observed in inflammatory diseases, indicating oxidative stress. Taurine protects cells from the cytotoxic effects of inflammation. There have been limited studies to date evaluating the effect of taurine in oxidative stress-induced vascular dysfunction a...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2016-04-01 00:00:00
abstract:OBJECTIVE:Long non-coding RNA CRNDE (CRNDE) recently emerged as a carcinogenic promoter in various cancers including gastric cancer (GC). However, the functions and molecular mechanisms of CRNDE to GC are still largely unclear. The aim of this study was to investigate the clinical significance and functional mechanisms...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201712_13925
更新日期:2017-12-01 00:00:00
abstract:OBJECTIVE:Intrauterine glucocorticoid (GC) exposure is associated with disturbances in feto-placental growth. This study aimed to investigate whether folic acid supplementation can prevent dexamethasone (Dex)-induced feto-placental growth restriction. MATERIALS AND METHODS:Female C57BL/6J mice were subject to four dif...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2015-04-01 00:00:00
abstract:BACKGROUND:In patients with chronic congenital haemolytic disorders, human Parvovirus B19 (HPV B19) is frequently involved in pure red-cell aplastic crises. Furthermore, it may inhibit three-lineage haematopoiesis in the bone marrow, causing severe pancytopenia. In such patients, Epstein Barr virus (EBV) infection also...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2012-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:2-aminoethoxydiphenyl borate (2-APB) has been reported to be a useful pharmacological tool in the study of calcium signaling. It is used either as a membrane-permeable inhibitor for inositol 1,4,5-trisphosphate (IP3) receptors or a store-operated calcium (Ca2+) entry (SOCE) inhibitor. The prese...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2012-08-01 00:00:00
abstract::This review illustrates some brief considerations of the medical use of cannabis recently issued in Italy. History and uses of cannabis throughout centuries and different countries are illustrated together with a description of botany and active phytocannabinoids. Then, medical use of cannabis anti-pain treatment for ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:10.26355/eurrev_201802_14405
更新日期:2018-02-01 00:00:00
abstract:OBJECTIVE:Precision medicine is a personalized disease prevention and treatment program combining modern genetic technology, molecular imaging techniques, and biological information with patients' living environment and clinical data, for accurate classification and diagnosis of diseases. CASE REPORT:Our study present...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2016-10-01 00:00:00
abstract::The prevalence of asthma and allergic diseases in childhood has increased in several industrialized countries since the second half of the twentieth century. In some countries, the prevalence is still rising, although in others it seems to have plateaued or even decreased. It has been suggested that environmental fact...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,收录出版,评审
doi:
更新日期:2016-01-01 00:00:00
abstract:OBJECTIVES:Lower respiratory tract infections (LRTIs), including pneumonia and acute exacerbations of Chronic Obstructive Pulmonary Disease (COPD), are among the most common diagnoses in both outpatient and inpatient settings. Due to the burden of LRTIs healthcare providers must adopt practices focused on improving out...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:This study aimed at exploring and contrasting the clinical significances and values of MRI, CT and contrast-enhanced ultrasonography in FIGO staging of cervical carcinoma. PATIENTS AND METHODS:The contrast-enhanced ultrasonography, CT and MRI imaging data of 348 patients with cervical carcinoma confirmed by ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201808_15612
更新日期:2018-08-01 00:00:00
abstract:OBJECTIVE:After MI pathological LV remodeling is one of the major causes of death. We previously showed the NO mediated beneficial effects of nebivolol in rat MI model, in this study we aimed to evaluate the NOS related mechanisms in this phenomenon. MATERIALS AND METHODS:Rats were divided into four groups: sham opera...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2015-12-01 00:00:00
abstract:OBJECTIVE:To explore the role of microRNA-326 in myocardial fibrosis after myocardial infarction (MI) and its underlying mechanism. MATERIALS AND METHODS:MI rat model was constructed via left anterior descending artery (LAD) ligation. Infarct tissues, infarct border zone tissues and remote zone tissues were harvested ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201808_15735
更新日期:2018-08-01 00:00:00
abstract:OBJECTIVE:Failure to identify patients in whom non-invasive ventilation (NIV) would be unsuccessful may cause inappropriate delay in intubation. Aim of this study was to determine predictors of NIV failure. PATIENTS AND METHODS:We retrospectively evaluated COPD patients, undergoing NIV for at least 12h because of hype...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2015-10-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to investigate clinical application of remifentanil in local anesthesia for resection of tumors in functional brain area. PATIENTS AND METHODS:Twenty-four patients were randomly divided into two groups: control group and remifentanil group. In remifentanil group remifentanil was inf...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,随机对照试验
doi:
更新日期:2014-01-01 00:00:00
abstract::The last two decades have witnessed two large-scale pandemics caused by coronaviruses, including severe acute respiratory syndrome (SARS) and the Middle East respiratory syndrome (MERS). At the end of 2019, another novel coronavirus, designated as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), hit Wuhan...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章,评审
doi:10.26355/eurrev_202004_21046
更新日期:2020-04-01 00:00:00
abstract:OBJECTIVE:The abnormal expression of lncRNA small nucleolar RNA host gene 7 (SNHG7) has been found in various kinds of cancers but its expression and role in breast cancer are unknown. We conducted this study to identify the expression, the function, and to underline the mechanism of SNHG7 in breast cancer. PATIENTS A...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201908_18545
更新日期:2019-08-01 00:00:00
abstract:OBJECTIVE:This study was made to investigate and evaluate the safety and carcinogenicity of nitenpyram (NIT) in rats. MATERIALS AND METHODS:A totally 50 male and 50 female SD rats were treated with NIT at 0, 800, 2400, and 7200 ppm, respectively, for 104 w. The growth, clinical signs, and survival rates, as well as th...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201812_16635
更新日期:2018-12-01 00:00:00
abstract:OBJECTIVE:MicroRNAs (miRNAs) are now recognized as important regulators of gene expression. The aim of the study was to investigate the role of miR-146b-5p in the TLR4 pathway and provide the basis for the treatment of lupus nephritis. MATERIALS AND METHODS:The glomerular mesangial cells were cultured in vitro and div...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201803_14589
更新日期:2018-03-01 00:00:00
abstract:BACKGROUND AND OBJECTIVES:Leishmania parasites are intracellular haemoflagellates that infect macrophages of the skin and viscera to produce diseases in their vertebrates hosts. Antileishmania therapy is based on pentavalent antimony compounds which toxicity of these agents and the persistence of side effects are sever...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2013-12-01 00:00:00
abstract:OBJECTIVE:Recent researches have revealed the role of long noncoding RNAs (lncRNAs) in tumor development. In this study, the potential function of lncRNA LUCAT1 in the progression of prostate cancer was identified. PATIENTS AND METHODS:Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) was used to detect LUCAT...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201904_17689
更新日期:2019-04-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to investigate the relationship between micro ribonucleic acid (miR)-152-3p and chronic myeloid leukemia (CML), and to explore the underlying mechanism. PATIENTS AND METHODS:The expression level of miR-152-3p in the bone marrow of 40 CML patients and 30 normal controls was detected ...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201812_16646
更新日期:2018-12-01 00:00:00
abstract:OBJECTIVE:To explore the influence of butorphanol on myocardial ischemia/reperfusion (I/R) injury in rats through the mitogen-activated protein kinase (MAPK) signaling pathway. MATERIALS AND METHODS:The I/R model in Sprague-Dawley rats was established. The rats were randomly divided into normal group (n=20), myocardia...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201912_19695
更新日期:2019-12-01 00:00:00
abstract:OBJECTIVE:This study examines the relationship between intracranial pressure (ICP) changes after skull injury repair and neurocognitive function before and after the repair. PATIENTS AND METHODS:Sixty patients undergoing skull injury repair participated in the study. A non-invasive detection analyzer was used to detec...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:
更新日期:2017-03-01 00:00:00
abstract:OBJECTIVE:Familial hypertrophic cardiomyopathy (HCM) is the most common genetic cardiac disease. While sarcomeric gene mutations explain many HCM cases, the genetic basis of about half of HCM cases remains elusive. Here we aimed to identify the gene causing HCM in a non-consanguineous Saudi Arabian family with affected...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_202007_22299
更新日期:2020-07-01 00:00:00
abstract:OBJECTIVE:p38 MAPK are a class of protein kinase that may induce or prevent apoptosis in different circumstance. Emerging researches show that it plays a vital role in tumor progression and therefore understanding its dual role in different stages of lung cancer are important to investigate. Also in this study, we plan...
journal_title:European review for medical and pharmacological sciences
pub_type: 杂志文章
doi:10.26355/eurrev_201809_15932
更新日期:2018-09-01 00:00:00
abstract::Inositol is a physiological compound belonging to the sugar family. The two inositol stereoisomers, myo-inositol and D-chiroinositol are the two main stereisomers present in our body. Myo-inositol is the precursor of inositol triphosphate, a second messenger regulating many hormones such as TSH, FSH and insulin. D-chi...
journal_title:European review for medical and pharmacological sciences
pub_type: 历史文章,杂志文章
doi:
更新日期:2014-07-01 00:00:00