Abstract：
:Inhibition of human phospholipases A2 by cis-unsaturated fatty acids and their oxidative metabolites and/or polymers was studied using partially purified human phospholipases A2 and [1-14C]oleate labelled, autoclaved E. coli as substrate. As previously reported for other phospholipases A2, oleic and arachidonic acids inhibited human synovial fluid phospholipase A2 with IC50s of 15 and 30 microM respectively. Air oxidation of arachidonic acid or hydroxylation of oleic acid (12-hydroxy-oleate) substantially relieved that inhibition. Similarly, the enzymatically oxidatized metabolite of arachidonate, prostaglandin B1 (PGB1), did not inhibit enzymatic activity. However, prostaglandin Bx (PGBx), an oligomer (n = 6) of PGB1, was a potent inhibitor of Ca(++)-dependent, neutral-active phospholipase A2 activities. Enzymatic activity in acid extracts from human neutrophils, platelets, sperm, plasma, synovial fluid, endometrium, degenerative disc, and snake venom was inhibited by PGBx with IC50s ranging from 0.5-7.0 microM. Inhibition was independent of substrate phospholipid concentration over a 24-fold range (5-120 microM) and PGBx quenched the tryptophan fluorescence of snake venom phospholipase A2 in a dose-dependent manner. Agonist-induced (A23187) release of arachidonic acid from prelabelled human neutrophils and cultured human endothelial cells was also inhibited by PGBx with IC50s of 3 and 20 microM, respectively. These results illustrate that oxidative reactions of cis-unsaturated fatty acids relieve their natural inhibitory activity, and polymerization of an inactive fatty acid metabolite yields a potent inhibitor of in vitro and in situ phospholipase A2 activity.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Franson R,Raghupathi R,Fry M,Saal J,Vishwanath B,Ghosh SS,Rosenthal MDdoi
10.1007/978-1-4613-0651-1_15subject
Has Abstractpub_date
1990-01-01 00:00:00pages
219-30eissn
0065-2598issn
2214-8019journal_volume
279pub_type
杂志文章abstract：:The effects of acute administration of either water or 2% NaCl solution via a stomach tube and injections of diurecits, furosemide (5, 10 mg) and acetazoleamide (5, 20 mg per rat), in adult rats upon urinary kallikrein (Kal), Na and K, were studied. Hyperhydration with water (5% b.w.) produced in 121% increase and 2% ...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4684-3267-1_43
更新日期：1976-01-01 00:00:00
abstract：:In contrast to heavily methylated mammalian genomes, invertebrate genomes are only sparsely methylated in a 'mosaic' fashion with the majority of methylated CpG dinucleotides found across gene bodies. Importantly, this gene body methylation is frequently associated with active transcription, and studies in the honeybe...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-319-43624-1_9
更新日期：2016-01-01 00:00:00
abstract：:Studies in late gestation fetal sheep have provided several new insights into the regulation of amniotic fluid (AF) volume (AFV): There are four quantitatively important amniotic inflows and outflows that include fetal urine production, lung liquid secretion, swallowing, and intramembranous absorption. Of these, AFV i...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4939-1031-1_5
更新日期：2014-01-01 00:00:00
abstract：:In the modern era of next-generation genomics and Fourth Industrial Revolution, there is a growing demand for translational research that brings about not only impactful research but also potential commercialisation of R- and D-based products. Advancement of metabolic engineering and synthetic biology has put forward ...
journal_title：Advances in experimental medicine and biology
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doi：10.1007/978-3-319-98758-3_6
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abstract：:The effects of sensory stimulation on the release of amino acids from sensori-motor and visual cortices have been studied using a superfusion technique. Electrical stimulation of the brachial plexus contra-lateral to the superfusion cannula increased significantly the release of glutamate and glutamine from the sensor...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4899-5199-1_15
更新日期：1979-01-01 00:00:00
abstract：:Inositol 1,4,5-trisphosphate receptors (IP3Rs) are ubiquitously expressed intracellular ligand-gated Ca2+ channels present on the endoplasmic reticulum of virtually all eukaryotic cells. These channels mediate the Ca2+ release from intracellular stores in response to activation by the signaling molecule IP3, which fun...
journal_title：Advances in experimental medicine and biology
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abstract：:Thermomechanical Analysis (TMA), using the parallel-plate-rheometer mode of the DuPont 943 TMA, was used to evaluate the softening properties of frozen aqueous carbohydrate model systems. A sample (of fixed dimension) of frozen solution was placed between the parallel plates and cooled to -80 degrees C. The sample was...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4899-0664-9_11
更新日期：1991-01-01 00:00:00
abstract：:Screening of recombinant cosmid and phage libraries with a rabbit C alpha cDNA probe has identified a total of seven C alpha genes organized into two clusters; one cluster contains four genes and the other cluster, three genes. Southern blot analysis of genomic DNA indicates that the rabbit genome contains a minimum o...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4684-5344-7_3
更新日期：1987-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
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更新日期：2018-01-01 00:00:00
abstract：:Curcumin, a major active component of turmeric, is extracted from the powdered dry rhizome of Curcuma longa Linn (Zingiberaceae) and it has been used for centuries in indigenous medicine. We have shown that curcumin has a protective role against myocardial necrosis in rats. The antioxidant activity of curcumin could b...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-0-387-46401-5_16
更新日期：2007-01-01 00:00:00
abstract：:Urinary tract infection (UTI) is common in children, causes them considerable discomfort, as well as distress to parents and has a tendency to recur. Approximately 20% of those children who experience one infection will have a repeat episode. Since 1975, 11 trials of long-term antibiotics compared with placebo or no t...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4614-4726-9_18
更新日期：2013-01-01 00:00:00
abstract：:The heart continuously supplies blood to the entire body throughout the lifetime, requiring a huge amount of the bioenergy molecule adenosine triphosphate (ATP). As the major subcellular organs that produce ATP through oxidative phosphorylation and the citrate-synthesis cycle, mitochondria inevitably produce chemicall...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-319-55330-6_32
更新日期：2017-01-01 00:00:00
abstract：:Immunotherapy entails the treatment of disease by modulation of the immune system. As detailed in the previous chapters, the different modes of achieving immune modulation are many, including the use of small/large molecules, cellular therapy, and radiation. Oncolytic viruses that can specifically attack, replicate wi...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-94-017-7555-7_5
更新日期：2016-01-01 00:00:00
abstract：:A characteristic feature of the spondyloarthropathies is inflammation at tendon or ligament attachment sites. This has traditionally been viewed as a focal abnormality, even though the inflammatory reaction intrinsic to enthesitis may be quite extensive. We argue that the diffuse nature of the pathology is best unders...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4419-0298-6_4
更新日期：2009-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4939-2981-8_73
更新日期：2016-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 历史文章,杂志文章,评审
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更新日期：2017-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-3-319-29073-7_1
更新日期：2016-01-01 00:00:00
abstract：:We describe the events surrounding the identification of the exosome complex and the subsequent early development of the field. Like many scientific discoveries, the initial identification and characterization ofthe exosome was based on a combination of skill, good fortune--and the availability of cutting edge technol...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：
更新日期：2010-01-01 00:00:00
abstract：:This study investigated the relationships between blood pressure, cortical oxygen pressure and hydroxyl radicals in the brain of adult cats during hemorrhagic hypotension and retransfusion. Oxygen pressure in the blood of the cortex was measured optically by the oxygen dependent quenching of phosphorescence and hydrox...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4615-4863-8_21
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abstract：:Prevalence of zoonotic Mycobacterium bovis (bTB) disease in human population is underreported from the North of Pakistan. Here, we report on the proportion of human bTB disease among the overall TB patients, drug resistance pattern of bTB isolates, and knowledge, attitude, and practices (KAP)-based analysis of bTB dis...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
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abstract：:The ultrastructural organization of elastin has been studied by scanning and transmission electron microscopy of negatively stained specimens coupled with optical diffraction analyses. Purified elastin results from a composite assembly of interwoven branched and twisted thread-like entities of decreasing diameter. The...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4684-9093-0_10
更新日期：1977-01-01 00:00:00
abstract：:Five human leukocyte long-term suspension cultures were investigated by means of cytochemical and cytogenetic methods. A striking resemblance in the morphology of these cells originating either from peripheral blood or from spleen of patients with or without hematological disorders was found. Lymphocytic origin of all...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4757-0731-1_15
更新日期：1975-01-01 00:00:00
abstract：:There is a direct correlation between the level of GBS beta-hemolysin expression and the ability of GBS to injury lung epithelial cells. Electron microscopy suggest the hemolysin acts as a pore-forming cytolysin. beta-hemolysin-associated lung epithelial cell injury is inhibited by surfactant phospholipid, a substance...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
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更新日期：1997-01-01 00:00:00
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更新日期：1998-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
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更新日期：2014-01-01 00:00:00
abstract：:This chapter deals with the classification of Drug Delivery nano Systems (DDnSs) with a Modulatory Controlled Release profile (MCR) denoted as Modulatory Controlled Release nano Systems (MCRnSs). Conventional (c) and advanced (a) DDnSs are denoted by the acronyms cDDnSs and aDDnSs, and can be composed of a single or m...
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abstract：:Transurethral resection (TUR) is the primary mode of therapy for both diagnosis and treatment of bladder cancer. Due to the recurrency of tumoral tissues after TUR further treatment is necessary which is usually in the form of intravesical chemotherapy or immunotherapy. But these therapies have some disadvantages such...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:Electroencephalography (EEG) studies in Alzheimer's Disease (AD) patients show an attenuation of average power within the alpha band (7.5-13 Hz) and an increase of power in the theta band (4-7 Hz). Significant body of evidence suggest that thalamocortical circuitry underpin the generation and modulation of alpha and t...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4614-0164-3_6
更新日期：2011-01-01 00:00:00
abstract：:Amphotropic retroviral vectors containing either the bacterial neomycin phosphotransferase gene or a mutant dihydrofolate reductase gene (DHFR*) were used to infect canine hematopoietic progenitor cells. Successful transfer and expression of both genes in canine hematopoietic progenitor cells has been achieved as meas...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4684-5571-7_3
更新日期：1988-01-01 00:00:00
abstract：:Do seizures change the brain? Studies on the kindling model--a widely used animal model of epilepsy--suggest that they do. Dr. Racine, one of the pioneers in the kindling field, describes the basic phenomena of kindling, and discusses the possible roles of cell growth and cell death in this model. ...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-1-4615-1335-3_16
更新日期：2002-01-01 00:00:00
