Abstract：
:Amphotropic retroviral vectors containing either the bacterial neomycin phosphotransferase gene or a mutant dihydrofolate reductase gene (DHFR*) were used to infect canine hematopoietic progenitor cells. Successful transfer and expression of both genes in canine hematopoietic progenitor cells has been achieved as measured by the ability of the viruses to confer resistance to either methotrexate (MTX) or the aminoglycoside G418, respectively. Gene transfer was achieved using helper-free retroviral vectors. The rate of gene expression in canine granulocyte/macrophage colony-forming units (CFU-GM) after cocultivation for 24 hours with virus-producing packaging cells ranged from 6-25%. Autologous marrow cocultivated for 24 hours with virus-producing packaging cells was transplanted into six dogs after lethal total body irradiation. All dogs showed engraftment within two weeks and four dogs survived for 5-7 months without adverse effects. One dog that had been given marrow infected with a DHFR* virus and that received MTX as in vivo selection after marrow transplantation and survived, showed 0.1 and 0.03% MTX-resistant CFU-GM at weeks 3 and 5. The efficiency of gene transfer into canine CFU-GM has been increased threefold by culturing marrow cells for six days in long-term marrow culture after 24 hour cocultivation with virus producing packaging cells.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Schuening F,Storb R,Nash R,Stead RB,Kwok WW,Miller ADdoi
10.1007/978-1-4684-5571-7_3subject
Has Abstractpub_date
1988-01-01 00:00:00pages
9-18eissn
0065-2598issn
2214-8019journal_volume
241pub_type
杂志文章abstract：:Key autonomic functions are in continuous development during adolescence which can be assessed using the heart rate variability (HRV). However, the influence of different demographic and physiological factors on HRV indices has not been fully explored in adolescents. In this study we aimed to assess the effect of age,...
journal_title：Advances in experimental medicine and biology
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journal_title：Advances in experimental medicine and biology
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doi：
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abstract：:Accumulating evidence suggests that sigma-1 receptors play a role in the pathophysiology of neuropsychiatric diseases, as well as in the mechanisms of some selective serotonin reuptake inhibitors (SSRIs). Among the SSRIs, the order of affinity for sigma-1 receptors is as follows: fluvoxamine > sertraline > fluoxetine ...
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abstract：:There is a growing awareness of the role of tropomyosin in the regulation of the actin filament. Work in the field is increasingly directed at understanding the mechanisms of function at both a molecular and atomic level and developing therapeutic strategies to treat tropomyosin-based pathology. This chapter highlight...
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journal_title：Advances in experimental medicine and biology
pub_type： 临床试验,杂志文章
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更新日期：1988-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
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更新日期：1998-01-01 00:00:00
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journal_title：Advances in experimental medicine and biology
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doi：10.1007/978-94-017-7215-0_15
更新日期：2015-01-01 00:00:00
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abstract：:Gestational diabetes mellitus is a hyperglycaemic state first recognised in pregnancy. GDM affects both mother and child. Women with GDM and their new-borns are at risk of developing type 2 diabetes in the future. The screening and diagnostic criteria for GDM are inconsistent and thus novel biomarkers of GDM are requi...
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更新日期：2017-01-01 00:00:00
abstract：:Immunotherapy entails the treatment of disease by modulation of the immune system. As detailed in the previous chapters, the different modes of achieving immune modulation are many, including the use of small/large molecules, cellular therapy, and radiation. Oncolytic viruses that can specifically attack, replicate wi...
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abstract：:In order to better understand the mechanisms of cerebrovascular development and differentiation, the leptomeningeal microcirculation was analyzed using in vivo fluorescence video-microscopy in neonatal (1, 9, and 12 day old) Sprague-Dawley rats. The pattern of flow distribution was reconstructed, the location of radia...
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更新日期：1998-01-01 00:00:00
abstract：:Mesenchymal stem cells (MSCs) are multipotent stem cells that reside in various organs. They have the capacity to differentiate into various cell types, including cardiomyocytes, vascular endothelial cells, and vascular smooth muscle cells. Among the various MSCs, bone marrow-derived MSCs (BMMSCs) have been widely use...
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abstract：:Bacteriophage play many varied roles in microbial ecology and evolution. This chapter collates a vast body of knowledge and expertise on Yersinia pestis phages, including the history of their isolation and classical methods for their isolation and identification. The genomic diversity of Y. pestis phage and bacterioph...
journal_title：Advances in experimental medicine and biology
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更新日期：2016-01-01 00:00:00
abstract：:During development in vivo and in vitro, estrogens: a) increase brain excitability, particularly in limbic structures; b) are responsible for the maturation and cyclicity of limbic-hypothalamic interrelations; c) enhance myelinogenesis; and d) may act with NGF to stimulate neurite formation. In senescence, estrogen ad...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章
doi：10.1007/978-1-4757-9551-6_19
更新日期：1997-01-01 00:00:00
abstract：:The dry antibiotic development pipeline coupled with the emergence of multi-drug resistant Gram-negative 'superbugs' has driven the revival of the polymyxin lipopeptide antibiotics. Understanding the mode of action of antibiotics is an important precursor for optimizing their use and development. This chapter provides...
journal_title：Advances in experimental medicine and biology
pub_type： 杂志文章,评审
doi：10.1007/978-3-030-16373-0_4
更新日期：2019-01-01 00:00:00
