Abstract:
:The present study sought to investigate the correlation between adipose cytokines (visfatin, leptin and adiponectin) and markers of multiple myeloma bone disease, and to determine the effects and mechanism of action of adiponectin on the differentiation and maturation of osteoclasts in multiple myeloma (MM). The levels of visfatin, leptin and adiponectin were measured. Their association with the indices of myeloma tumor load and bone disease were analyzed. Reverse transcription‑quantitative PCR was used to detect the expression of receptor activator of nuclear factor‑κB ligand (RANKL), osteoclast associated Ig‑like receptor (OSCAR), tartrate‑resistant acid phosphatase (TRAP) and Cathepsin K genes. Flow cytometry was used to detect the expression of adiponectin receptor 1 (AdipoR1) and the phosphorylation of the mechanistic target of rapamycin kinase (mTOR) pathway‑associated proteins mTOR and eukaryotic translation initiation factor 4E‑binding protein (4EBP1). There were no significant correlations among leptin, visfatin and the indexes of myeloma tumor load and bone disease. Serum adiponectin levels were significantly lower in patients with newly diagnosed multiple myeloma compared with healthy volunteers (12.37±3.13 vs. 13.80±0.95; P<0.05). The number of mature osteoclasts in the adiponectin group was lower compared with in the control group. Adiponectin also inhibited the mRNA expression of the osteoclast‑associated factors RANKL, OSCAR, TRAP and Cathepsin K. Comparison between the non‑adiponectin group and the adiponectin group revealed that adiponectin increased the expression of AdipoR1 on the surface of osteoclast precursor cells (26.21±4.27% vs. 29.86±6.23%; P<0.05) and reduced the expression of phosphorylated (p‑)mTOR (7.89±1.00% vs. 5.91±1.26%; P<0.05) and p‑4EBP1 (26.78±5.00% vs. 22.49±4.24%; P<0.05). The p‑mTOR and p‑4EBP1 levels in the adiponectin + MHY1485 (an mTOR signaling pathway‑specific agonist) group were significantly higher compared with those in the adiponectin group. It was revealed that adiponectin may inhibit osteoclast differentiation and maturation via the mTOR pathway. In conclusion, adiponectin inhibits the differentiation and maturation of osteoclasts by increasing the expression of AdipoR1 and reducing the phosphorylation levels of mTOR and 4EBP1 in patients with MM.
journal_name
Int J Mol Medjournal_title
International journal of molecular medicineauthors
Liu Z,Liu H,Li Y,Wang Y,Xing R,Mi F,Xiang C,Fu Rdoi
10.3892/ijmm.2020.4475subject
Has Abstractpub_date
2020-04-01 00:00:00pages
1112-1120issue
4eissn
1107-3756issn
1791-244Xjournal_volume
45pub_type
杂志文章abstract::The proliferation and migration of vascular smooth muscle cells (SMC) are important aspects of atherogenesis. Activated growth factor signaling in injured vessels subsequently promotes a number of intracellular events resulting in the phenotypic modulation of SMC. Here, we investigated the role of beta2-chimaerin, a n...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:
更新日期:2006-04-01 00:00:00
abstract::In the absence of antibodies specific for lymphatic vessels, analysis of lymphatic vessels within different tissues has been widely performed with light microscopic and, most importantly, electron microscopic techniques. In regard to lymphatic vessels in the ocular globe and the periocular structures, controversy rema...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:10.3892/ijmm.13.5.681
更新日期:2004-05-01 00:00:00
abstract::Thioredoxin domain‑containing 5 (TXNDC5) is reportedly overexpressed in colorectal cancer (CRC) and is therefore considered an oncogene. However, the role of TXNDC5 in CRC tumorigenesis remains unclear. The present study aimed to explore the role of TXNDC5 in CRC tumorigenesis in vitro and in vivo under hypoxic and no...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:10.3892/ijmm.2018.3664
更新日期:2018-08-01 00:00:00
abstract::Metastasis is a hallmark of malignant neuroblastoma and is the main reason for therapeutic failure and recurrence of the tumor. The CXC chemokine receptor-4 (CXCR4), a Gi protein-coupled receptor for the ligand CXCL12/stromal cell-derived factor-1α (SDF-1α), is expressed in various types of tumor. This receptor mediat...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:10.3892/ijmm.2014.2032
更新日期:2015-02-01 00:00:00
abstract::Fas antigen, also termed APO-1 or CD95, is a transmembrane protein and a member of the tumor necrosis factor receptor/nerve growth factor receptor superfamily which mediates apoptosis upon oligomerization. The Fas/Fas ligand system is considered to be a key regulator of apoptosis. Recently, we have demonstrated that F...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:10.3892/ijmm.2.4.435
更新日期:1998-10-01 00:00:00
abstract::Wiskott-Aldrich syndrome (WAS) is an X-linked recessive disorder characterized by microthrombocytopenia, eczema, immunodeficiency, and susceptibility to lymphoid malignancy. Loss-of-function mutations in WAS gene have been identified to cause disorders with platelet defects including WAS and X-linked thrombocytopenia....
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:
更新日期:2003-12-01 00:00:00
abstract::MicroRNAs (miRNAs) have important effects on cancer occurrence and development by adjusting gene expression. The aim of the present study was to examine the role of miR‑214 in papillary thyroid carcinoma cell proliferation and metastasis, and its molecular mechanisms. miR‑214 was demonstrated to be markedly downregula...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:10.3892/ijmm.2018.3902
更新日期:2018-12-01 00:00:00
abstract::Synovial membrane consists of fibroblasts and macrophages forming the synovial lining supported by vascularized subsynovium. Each of these components may specifically react to a particular stimulus. Thus, reactions of isolated synovial cells may not correspond to that of intact tissue. We characterized the production ...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:10.3892/ijmm_00000268
更新日期:2009-10-01 00:00:00
abstract::The colonic crypt contains highly proliferative cells in its base and differentiated cells on its luminal surface. Carcinogenesis significantly affects this orderly cellular distribution. The aims of this study were: i) to examine the expression of apoptosis-related proteins along the crypt-lumen axis during 1, 2-dime...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:10.3892/ijmm.6.6.689
更新日期:2000-12-01 00:00:00
abstract::This study examined the mechanism for the anti-cancer effects of histone deacetylase (HDAC) inhibitor trichostatin A (TsA) in H-ras-transformed human breast epithelial (MCF10A-ras) cells. The effects of TsA on anti-cancer effects of MCF10A-ras cells were determined by measuring the level of cell cycle regulator expres...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:
更新日期:2008-11-01 00:00:00
abstract::Serum response factor (SRF) is a transcription factor that has important roles in tumor progression. However, its role in cervical cancer cell proliferation and invasion remains unclear. The present study revealed that SRF silencing constrained cervical cancer cell proliferation and invasion via controlling early grow...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:10.3892/ijmm.2018.3954
更新日期:2019-01-01 00:00:00
abstract::ABCA3 is a surfactant lipid transporter in the limiting membrane of lamellar bodies in alveolar type II cells. Mutations in the ATP-binding cassette, sub-family A (ABC1), member 3 (ABCA3) gene cause respiratory distress syndrome in newborns, and chronic interstitial lung disease in children and adults. ABCA3 belongs t...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:10.3892/ijmm.2016.2650
更新日期:2016-08-01 00:00:00
abstract::Astragaloside IV is a monomer isolated from Astragalus membranaceus (Fisch.) Bunge, which is one of the most widely used plant-derived drugs in traditional Chinese medicine for diabetes therapy. In the present study, we aimed to examine the effects of astragaloside IV on glucose in C2C12 myotubes and the underlying mo...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:10.3892/ijmm.2016.2555
更新日期:2016-06-01 00:00:00
abstract::This study investigated the effects of mycelial extract and culture filtrate of Cordyceps sphecocephala J201 on airway hyper-responsiveness, pulmonary immune cell infiltration, and Th2 cytokine expression in animal models of asthma. After Concanavalin A (Con A) activation of mouse primary spleen cells, the IL-4 and IL...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:
更新日期:2010-09-01 00:00:00
abstract::Poligoni Multiflori Radix (PMR) is a traditional Korean medicinal herb that is known to have various pharmacological effects, including antihyperlipidemic, anticancer, and anti‑inflammatory effects. However, the effects of PMR on bone metabolism have not been elucidated to date. The present study aimed to investigate ...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:10.3892/ijmm.2018.3603
更新日期:2018-07-01 00:00:00
abstract::Acute lung injury (ALI) is among the most common causes of mortality in intensive care units. Recent in vivo and in vitro studies have suggested that mesenchymal stem cells (MSCs) attenuate pulmonary edema and inflammatory factors, but the mechanisms of the effects of MSCs on pulmonary vascular function remain unknown...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:10.3892/ijmm.2013.1328
更新日期:2013-06-01 00:00:00
abstract::Our earlier reports indicated that the changes of age-adjusted incidence rates (AAIRs) of any 2 tumors in time and space, as investigated by the sequential regression analysis, showed a good fitness to the equilibrium model under the control of the law of mass action. The purpose of this study is to investigate the pr...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:10.3892/ijmm.3.4.435
更新日期:1999-04-01 00:00:00
abstract::In the present study, the chemoprotective effects of recombinant Pyropia yezoensis (P. yezoensis) protein 1 (PYP1) were examined in acetaminophen (APAP)-treated Chang liver cells. The analysis of P. yezoensis revealed the presence of both mature and immature variants of PYP1. PYP1s, designated as PYP1 (15 kDa), PYP1-A...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:10.3892/ijmm.2015.2253
更新日期:2015-08-01 00:00:00
abstract::Hyposalivation is an intractable side‑effect of radiotherapy for head and neck cancer. It is caused by the irreversible loss of acinar cells and decreased saliva secretion. However, this situation severely compromises the quality of life of affected patients. Currently, there is no effective treatment for this conditi...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:10.3892/ijmm.2014.1837
更新日期:2014-09-01 00:00:00
abstract::Free radicals derived from the reaction of iron and oxygen are thought to be one of the causes of tissue injury. In order to identify whether oxygen concentrations are an important factor in iron-mediated damage to cells, cytotoxic effects of Fe(3+)-NTA on human fibroblasts (KMST-6 line) were studied under the conditi...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:
更新日期:2001-03-01 00:00:00
abstract::Currently, several therapeutic approaches including surgery, chemotherapy, and radiation therapy are available for the treatment of endometrial cancer. However, endometrial cancer cells may survive, resulting in relapse of the disease, and ultimately causing demise of the patient. Hepsin is a cell surface-expressed ch...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:
更新日期:2008-09-01 00:00:00
abstract::The basic helix loop helix (bHLH) transcription factor DEC2 is associated with the regulation of apoptosis, circadian rhythm and the response to hypoxia. However, the significance of DEC2 in pancreatic cancer remains unknown. Here, we showed for the first time that DEC2 inhibits the...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:10.3892/ijmm.2012.1037
更新日期:2012-09-01 00:00:00
abstract::Annexin A9 (ANXA9), a member of annexin family, has been reported be associated with colorectal cancer (CRC) carcinogenesis. However, the clinical significance of ANXA9 in CRC, particularly its correlation to invasion and metastasis remains ambiguous. The aim of the present study was to investigate the significance of...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:10.3892/ijmm.2018.3432
更新日期:2018-04-01 00:00:00
abstract::Determination of CD34+ cells was performed in bone marrow and G-CSF mobilised peripheral blood samples. We adopted three different protocols of analysis: the Milan/Mulhouse protocol, the ISHAGE guidelines for CD34+ cell determination and our own protocol based upon the use of PAINT-A-GATEPRO software analysis program....
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:10.3892/ijmm.1.1.67
更新日期:1998-01-01 00:00:00
abstract::Recently a candidate gene for hereditary hemo-chromatosis, HFE, was identified. The finding raises the possibility for genetic testing to provide earlier detection and more complete genotypic evaluation of hemochromatosis affected individuals. We determined the frequency of the HFE polymorphisms, C282Y and H63D, in a ...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:10.3892/ijmm.4.4.389
更新日期:1999-10-01 00:00:00
abstract::Cell numbers are regulated by a balance between proliferation and apoptosis (programmed cell death). Recent evidence suggests that proteins regulating cell proliferation also mediate apoptosis. Therefore, cellular fate might be determined by cross talk between regulators of cell cycle progression and apoptosis. Previo...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:10.3892/ijmm.1.1.131
更新日期:1998-01-01 00:00:00
abstract::Tubular atrophy and dysfunction is a critical process underlying diabetic nephropathy (DN). Understanding the mechanisms underlying renal tubular epithelial cell survival is important for the prevention of kidney failure associated with glucotoxicity. Autophagy is a cellular pathway involved in protein and organelle d...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:10.3892/ijmm.2014.2052
更新日期:2015-03-01 00:00:00
abstract::The emergence of colorectal cancer in developed nations can be attributed to dietary habits, smoking, a sedentary lifestyle and obesity. Several treatment regimens are available for primary and metastatic colorectal cancer; however, these treatment options have had limited impact on cure and disease‑free survival, and...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:10.3892/ijmm.2019.4284
更新日期:2019-10-01 00:00:00
abstract::The cellular events leading to cerebral vasospasm after subarachnoid hemorrhage (SAH) are poorly understood, although the family of protein kinase C (PKC) is already known to play crucial roles in this pathology. Hemoglobin (Hb) is one of the major causes of the cerebral vasospasm that follows SAH. In the present stud...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:
更新日期:2007-10-01 00:00:00
abstract::Gene therapy is becoming an important treatment modality against various diseases including cancer, genetic disorders, infectious diseases and inflammatory diseases. For achieving successful gene therapy, it is extremely important to control the expression of a transduced therapeutic gene. However, there have been few...
journal_title:International journal of molecular medicine
pub_type: 杂志文章
doi:
更新日期:2002-07-01 00:00:00