Abstract：
:Fas antigen, also termed APO-1 or CD95, is a transmembrane protein and a member of the tumor necrosis factor receptor/nerve growth factor receptor superfamily which mediates apoptosis upon oligomerization. The Fas/Fas ligand system is considered to be a key regulator of apoptosis. Recently, we have demonstrated that Fas antigen expression is induced by low-dose irradiation of some types of lymphomas, and we also demonstrated that irradiation-induced Fas antigen expression increased with the passage of time until peaking at 48 h after irradiation in CML-C1, CML-C2, DL-40, and DL-95 cell lines. In this study, we also examined the potential cytotoxicity of Fas ligand peptide against several types of lymphoma/leukemia cell lines that showed induction of Fas antigen expression under irradiation. Flow cytometry analysis was performed at 6, 24 and 48 h after irradiation. Samples (1 x10(6) cells/ml) from irradiated and non-irradiated cells of each cell line were incubated with or without 5 microg/ml of Fas ligand peptide for 2 h at 37 degrees C in a humidified atmosphere of 5% carbon dioxide (CO2) in air. The killing effect of Fas ligand against cell lines of CML-C1, DL-40, and DL-95 were clearly identified as the percentage of cells with Fas antigen expression induced by irradiation. Concerning HD-70 cell line, for which soluble Fas antigen has been identified, the killing effects were clearly observed in samples pre-treated with PBS washings. To our knowledge, this is the first report describing a possible application of the Fas/Fas ligand system in treatment of certain types of malignancies in which Fas antigen is inducible by irradiation.
journal_name
Int J Mol Medjournal_title
International journal of molecular medicineauthors
Ogawa Y,Nishioka A,Kubonishi I,Inomata T,Yoshida S,Kataoka Sdoi
10.3892/ijmm.2.4.435subject
Has Abstractpub_date
1998-10-01 00:00:00pages
435-6issue
4eissn
1107-3756issn
1791-244Xjournal_volume
2pub_type
杂志文章abstract：:Aberrant glycosylation is a universal feature of cancer cells. There are quantitative and qualitative changes in expression of gangliosides observed in tumors of a neuroectodermal origin such as neuroblastoma, melanoma and astrocytoma. The presence of large amounts of GD2 ganglioside on neuroblastoma cells, as compare...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2007-05-01 00:00:00
abstract：:The membrane cytoskeletal component dystrophin and its associated glycoproteins play a central role in the molecular pathogenesis of several muscular dystrophies, i.e. Duchenne/Becker muscular dystrophy, congenital muscular dystrophy and various forms of limb-girdle muscular dystrophy. Although the most frequent of th...
journal_title：International journal of molecular medicine
pub_type： 杂志文章,评审
doi：10.3892/ijmm.2.6.639
更新日期：1998-12-01 00:00:00
abstract：:Nonalcoholic fatty liver disease (NAFLD) is one of the most frequent causes of abnormal liver dysfunction, and its prevalence has markedly increased. We previously evaluated the expression of fatty acid metabolism-related genes in NAFLD and reported changes in expression that could contribute to increased fatty acid s...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2007-09-01 00:00:00
abstract：:We had shown previously that the novel, marine, anticancer compound dehydrothyrsiferol (DHT) does not modulate P-glycoprotein (P-gp) dependent drug efflux. Many chemotherapeutics with clinical impact are substrates for the structurally distant related membrane transport protein MRP1 (multidrug resistance-associated pr...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2002-11-01 00:00:00
abstract：:Endoplasmic reticulum (ER) stress plays a vital role in mediating ischemic reperfusion damage in brain. In this study, we evaluated whether melatonin inhibits ER stress in cultured neurons exposed to oxygen and glucose deprivation (OGD) and in rats subjected to transient focal cerebral ischemia. Sprague-Dawley rats we...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2018.3607
更新日期：2018-07-01 00:00:00
abstract：:Poligoni Multiflori Radix (PMR) is a traditional Korean medicinal herb that is known to have various pharmacological effects, including antihyperlipidemic, anticancer, and anti‑inflammatory effects. However, the effects of PMR on bone metabolism have not been elucidated to date. The present study aimed to investigate ...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2018.3603
更新日期：2018-07-01 00:00:00
abstract：:High-fidelity maintenance of genomic integrity in eukaryotes is ensured by cell cycle checkpoints and DNA repair. The checkpoint kinase, Chk2, has been implicated in both of these responses. In response to DNA damage, Chk2 is initially phosphorylated at Thr-68, which leads to its full activation. The fully activated C...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2006-05-01 00:00:00
abstract：:Porphyra yezoensis (P. yezoensis) is the most noteworthy red alga and is mainly consumed in China, Japan and Korea. In the present study, the effects of a P. yezoensis peptide (PY‑PE) on cell proliferation and the associated signaling pathways were examined in IEC‑6 rat intestinal epithelial cells. First, the MTS assa...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2014.2037
更新日期：2015-02-01 00:00:00
abstract：:Intermittent low‑dose injections of parathyroid hormone (PTH) have been reported to exert bone anabolic effects and to promote fracture healing. As an important proangiogenic cytokine, vascular endothelial growth factor (VEGF) is secreted by bone marrow mesenchymal stem cells (BMSCs) and osteoblasts, and serves a cruc...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2018.3614
更新日期：2018-07-01 00:00:00
abstract：:Inflammatory genes serve a crucial role in the pathogenesis of inflammation‑associated tumors. However, as recent studies have mainly focused on the effects of single inflammatory genes on colorectal cancer (CRC), but not on the global interactions between genes, the underlying mechanisms between inflammatory genes and...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2018.3631
更新日期：2018-07-01 00:00:00
abstract：:The aim of this study was to investigate the therapeutic effects of three different cellulose membranes (CMs) manufactured from Styela clava tunics (SCTs) on the healing of cutaneous wounds. We examined the physical properties and therapeutic effects of three CMs regenerated from SCTs (referred to as SCT‑ CMs), includ...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2017.2923
更新日期：2017-05-01 00:00:00
abstract：:Arachidin-1 [trans-4-(3-methyl-1-butenyl)-3,5,3',4'-tetrahydroxystilbene] is a polyphenol produced by peanut kernels during germination. The aim of the present study was to investigate the mechanism underlying the anti-inflammatory effect of arachidin-1 in endothelial cells (ECs). The results of cell adhesion and west...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2017.3238
更新日期：2018-01-01 00:00:00
abstract：:Curcumin is an orange-yellow colored, lipophilic polyphenol substance derived from the rhizome of Curcuma longa that is widely used in many countries. Curcumin has many reported functions, including antioxidant and anti‑inflammatory effects. Autophagy removes damaged organelles and protein aggregates in the cell. Howe...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2018.3847
更新日期：2018-11-01 00:00:00
abstract：:Neural stem cell transplantation is a promising new treatment of ischemic or traumatic brain injury. We have now investigated the involvement of the peroxynitrite - poly(ADP-ribose) polymerase (ONOO- - PARP) activation cascade in brain trauma and neural stem cell transplantation. The forelimb motor cortex of adult mal...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2003-08-01 00:00:00
abstract：:Cardiac fibroblast (CF) proliferation and transformation into myofibroblasts play important roles in cardiac fibrosis during pathological myocardial remodeling. In this study, we demonstrate that hepatocyte growth factor (HGF), an antifibrotic factor in the process of pulmonary, renal and liver fibrosis, is a negative...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2014.1782
更新日期：2014-08-01 00:00:00
abstract：:Xuebijing injection (XBJ) has long been used to treat infectious diseases in China. The therapeutic effect of XBJ is probably associated with anti-inflammatory effects. However, the precise mechanisms responsible for the effects of XBJ remain unknown. The present study was conducted in order to evaluate the protective...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2016.2749
更新日期：2016-11-01 00:00:00
abstract：:Gefitinib, an epidermal growth factor receptor tyrosine kinase inhibitor, has been frequently used in targeted therapy for lung cancer. However, the widespread use of gefitinib in targeted therapy for patients with lung cancer is hampered by its common skin toxicities. The present study aimed to investigate the mechan...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2018.4046
更新日期：2019-03-01 00:00:00
abstract：:Reactive oxygen species (ROS), generated either extracellularly or intracellularly through ligand-receptor interactions, can function as signal transduction molecules to activate the chemotactic cytokine interleukin-8 (IL-8) and the cell surface adhesion protein, intercellular adhesion molecule-1 (ICAM-1; CD54). Toget...
journal_title：International journal of molecular medicine
pub_type： 杂志文章,评审
doi：10.3892/ijmm.4.3.223
更新日期：1999-09-01 00:00:00
abstract：:The deletion mutation of exon 4 in surfactant protein C (SP-C), a lung surfactant protein, has been identified in parent-child cases of familial interstitial pneumonia. It has been shown that this mutation induces endoplasmic reticulum (ER) stress. Synoviolin is an E3 ubiquitin ligase that is localized to the ER and i...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2014.1984
更新日期：2015-01-01 00:00:00
abstract：:The aim of the present study was to determine the correlation between angiogenesis and the differential expression of growth factors and their receptors when myocardial microvascular endothelial cells (MMVECs) were co-cultured with mast cell granules (MCGs) under hyperglycemic conditions. MMVECs and mast cells (MCs) w...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2013.1293
更新日期：2013-05-01 00:00:00
abstract：:The tumor microenvironment is thought to affect malignant transformation and tumor progression. The histone methyltransferase, enhancer of zeste homologue 2 (EZH2), has recently been suggested to play a critical role in the tumorigenesis of several types of human cancer. The aim of this study was to investigate the ef...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2013.1549
更新日期：2014-01-01 00:00:00
abstract：:Mycobacterium tuberculosis (MTB), one of the major bacterial pathogens for lethal infectious diseases, is capable of surviving within the phagosomes of host alveolar macrophages; therefore, host genetic variations may alter the susceptibility to MTB. In this study, to identify host genes exploited by MTB during infect...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2016.2675
更新日期：2016-09-01 00:00:00
abstract：:Exposure of the skin to ultraviolet (UV) radiation causes extracellular matrix (ECM) collapse in the dermis, owing to an increase in matrix metalloproteinase (MMP) production in both the epidermis and dermis, and a decrease in type I collagen expression in the dermis. Recently, black rice (Oryza sativa L.) was reporte...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2018.3508
更新日期：2018-05-01 00:00:00
abstract：:Microglia of the central nervous system serve a variety of functions that may ultimately lead to the development or detriment of neighboring neuronal and vascular cells. These scavengers of the nervous system have been associated with a variety of neurodegenerative disorders, but the toxic potential of microglia is eq...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2007-02-01 00:00:00
abstract：:When treating glaucoma, excessive scar tissue reactions reduce the postoperative survival rate of the filtering bleb. Accumulating evidence has demonstrated that the proliferation, migration and extracellular matrix (ECM) synthesis of fibroblasts are important molecular mechanisms underlying scar formation. Recent evi...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2017.3323
更新日期：2018-03-01 00:00:00
abstract：:Tissue engineering represents a promising method for generating chondrogenic grafts for reconstructive surgery. In cultured chondrocytes, the dedifferentiation of cells seems unavoidable for multiplication. Stem cells, however, displaying unlimited self-renewal and the capacity to differentiate towards chondrocytes, m...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2006-02-01 00:00:00
abstract：:An effective therapeutic strategy for suppressing liver fibrosis development should improve the overall prognosis of patients with chronic liver diseases. Despite efforts to develop anti-fibrotic agents, no drugs have yet been approved as anti-fibrotic treatments for humans. An alternative strategy may be to employ a ...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2011.658
更新日期：2011-07-01 00:00:00
abstract：:Apoptosis plays a critical role in the pathogenesis of chronic myocardial ischemia (CMI) and heart failure (HF). Endoplasmic reticulum stress (ERS) is one of the newly defined signaling pathways which initiate apoptosis. Previous studies have shown that ERS-associated apoptosis is involved in the pathogenesis of HF in...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：10.3892/ijmm.2011.612
更新日期：2011-04-01 00:00:00
abstract：:Atrial natriuretic peptide (ANP) is a regulatory hormone widely expressed, along with its receptors, in organs and body tissues. ANP is well known to inhibit aldosterone secretion from mammalian adrenals, but its effect on glucocorticoid-hormone production is controversial. In vivo experiments showed that prolonged AN...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2006-04-01 00:00:00
abstract：:Smoking has deleterious effects on osteoporosis and periodontitis both characterized by bone loss. Smoking also interferes with the protective effect that hormone replacement therapy (HRT) has on bone loss. Our study investigated two mechanisms by which smoking may affect bone metabolism: nicotine-induced proliferatio...
journal_title：International journal of molecular medicine
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
