Abstract：
:A sensitive and simple liquid chromatography-mass spectrometry (LC-MS) method has been developed and validated for the quantification of solamargine, a steroidal glycoalkaloid, in rat plasma. Vincristine was selected as the internal standard. Sample preparation involved simple liquid-liquid extraction by ethyl acetate with high efficiency. The chromatographical separation was performed on a Shimadzu C(18) column (150mm×2.0mm, 5μm) with a gradient elution of acetonitrile and 0.02% (v/v) formic acid. The elutes were detected under positive electrospray ionization (ESI) and the target analytes quantified by selected ion monitoring (SIM) mode. The method was sensitive with the lowest limit of quantitation (LLOQ) at 0.5ng/mL in 50μL of rat plasma. Good linearity (r(2)=0.9996) was obtained covering the concentration of 0.5-2000.0ng/mL. The intra- and inter-day assay precision ranged from 2.87 to 3.60% and 0.52 to 6.81%, respectively. In addition, the stability, extraction recovery and matrix effect involved in the method were also validated. The practical utility of the aforementioned method was successfully confirmed in the pharmacokinetic evaluation of solamargine in Sprague-Dawley rats after intravenous administration.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Zheng X,Xu L,Liang Y,Xiao W,Xie L,Zhang Y,Zhao L,Cao L,Chen J,Wang Gdoi
10.1016/j.jpba.2011.04.007subject
Has Abstractpub_date
2011-07-15 00:00:00pages
1157-62issue
5eissn
0731-7085issn
1873-264Xpii
S0731-7085(11)00205-6journal_volume
55pub_type
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