A rapid and sensitive UHPLC-MS/MS method for quantification of 2-(2-hydroxypropanamido) benzoic acid in rat plasma: application to a pharmacokinetic study.


:A rapid, sensitive and high throughput UHPLC-MS/MS method was established and validated to assay the concentration of 2-(2-hydroxypropanamido) benzoic acid (HPABA), a promising anti-inflammatory drug, in rat plasma. Plasma samples were processed by liquid-liquid extraction with ethyl acetate and separated on a Shim-pack XR-ODS C18 column (75 mm×3.0 mm, 2.2 μm) at an isocratic flow rate of 0.4 mL/min using acetonitrile-0.1% formic acid in water (50:50, v/v) as mobile phase, and total run time was 2 min. MS/MS detection was accomplished in multiple reaction monitoring (MRM) mode with positive electrospray ionization. The calibration curve was linear over the concentration range of 0.01-50 μg/mL with lower limit of quantification of 0.01 μg/mL. The intra- and inter-day precisions were below 8.5% in terms of relative standard deviation (RSD), and the accuracy was within ±4.0% in terms of relative error (RE). Extraction recovery, matrix effect and stability were satisfactory in rat plasma. The developed method was successfully applied to a pharmacokinetic study of HPABA following intragastric administration of 25, 50, 100mg/kg and an intravenous injection at a dose of 12.5 mg/kg to Sprague-Dawley rats. Results indicated that HPABA had linear pharmacokinetic properties within the tested intragastric dosage range and the absolute bioavailability was above 59.1%.


J Pharm Biomed Anal


Guan J,Zhao Y,Zhu H,An Z,Yu Y,Li R,Yu Z




Has Abstract


2014-07-01 00:00:00












  • GC-MS fingerprint of Pogostemon cablin in China.

    abstract::Pogostemon cablin, originating in Malaysia and India, is cultivated in southern China including Guangdong and Hainan Province, which was called GuangHuoXiang to differentiate it from the HuoXiang of the north, the species Agastache rugosa, that it resembles. Essential oil of P. cablin mainly contributes to the pharmac...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章


    authors: Hu LF,Li SP,Cao H,Liu JJ,Gao JL,Yang FQ,Wang YT

    更新日期:2006-09-18 00:00:00

  • LC, LC-MS/TOF and MS(n) studies for the identification and characterization of degradation products of nelfinavir mesylate.

    abstract::The objective of the present investigation was to separate, identify and characterize the major degradation products (DPs) of nelfinavir mesylate generated under hydrolytic, oxidative, photolytic and thermal stress conditions as advised in International Conference on Harmonization (ICH) guideline Q1A(R2). The drug was...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章


    authors: Tiwari RN,Bonde CG

    更新日期:2011-06-01 00:00:00

  • Recent advancements, challenges, and practical considerations in the mass spectrometry-based analytics of protein biotherapeutics: A viewpoint from the biosimilar industry.

    abstract::The extensive analytical characterization of protein biotherapeutics, especially of biosimilars, is a critical part of the product development and registration. High-resolution mass spectrometry became the primary analytical tool used for the structural characterization of biotherapeutics. Its high instrumental sensit...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章,评审


    authors: Háda V,Bagdi A,Bihari Z,Timári SB,Fizil Á,Szántay C Jr

    更新日期:2018-11-30 00:00:00

  • In situ monitoring of powder blending by non-invasive Raman spectrometry with wide area illumination.

    abstract::A 785nm diode laser and probe with a 6mm spot size were used to obtain spectra of stationary powders and powders mixing at 50rpm in a high shear convective blender. Two methods of assessing the effect of particle characteristics on the Raman sampling depth for microcrystalline cellulose (Avicel), aspirin or sodium nit...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章


    authors: Allan P,Bellamy LJ,Nordon A,Littlejohn D,Andrews J,Dallin P

    更新日期:2013-03-25 00:00:00

  • Characterization of the acidic species of a monoclonal antibody using free flow electrophoresis fractionation and mass spectrometry.

    abstract::Antibody charge heterogeneity is one of the major product-related variants in recombinant biopharmaceuticals, which has been commonly monitored by imaged capillary isoelectric focusing (icIEF). Due to the challenges with sample recovery and fractionation, other charge-based analytical approaches have been explored as ...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章


    authors: Liu S,Madren S,Feng P,Sosic Z

    更新日期:2020-06-05 00:00:00

  • Photolysis of cyanocobalamin in aqueous solution.

    abstract::Cyanocobalamin is photolysed in aqueous solution to produce hydroxocobalamin. The kinetics of photolysis has been studied at pH 1-12 using a newly developed spectrophotometric method for the simultaneous determination of the two compounds at 550 and 525 nm or 361 and 351 nm. Cyanocobalamin follows zero-order kinetics ...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章


    authors: Ahmad I,Hussain W,Fareedi AA

    更新日期:1992-01-01 00:00:00

  • Rapid analysis of benzodiazepines in whole blood by high-performance liquid chromatography: use of a monolithic column.

    abstract::In a previous work [J. Pharm. Biomed. Anal. 23 (2000) 447] a rapid high-performance liquid chromatography (HPLC) method, using a monolithic column in HPLC coupled with a diode-array detector, was developed for the quantitative determination of benzodiazepines in whole blood. The present method has been applied to the ...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章


    authors: Bugey A,Staub C

    更新日期:2004-05-28 00:00:00

  • SPE-HPLC method for the determination and pharmacokinetic studies on paeoniflorin in rat serum after oral administration of traditional Chinese medicinal preparation Guan-Xin-Er-Hao decoction.

    abstract::A new HPLC method for the determination of paeoniflorin in rat serum with solid-phase extraction (SPE) for preconcentration is introduced. Paeoniflorin and an internal standard (pentoxifylline) were extracted from serum by means of SPE using cartridges with octadecyl chemically bound phase. The HPLC separation was the...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章


    authors: Ye G,Li YZ,Li YY,Guo HZ,Guo DA

    更新日期:2003-10-15 00:00:00

  • Capillary zone electrophoresis of minocycline.

    abstract::A method is described for analysis of the tetracycline antibiotic minocycline using capillary zone electrophoresis. Potential impurities are 4-epiminocycline, 6-deoxy-6-demethyltetracycline, 7-didemethylminocycline, 7-monodemethylminocycline and 9-minocycline. Method development was undertaken with a mixture consistin...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章


    authors: Li YM,Van Schepdael A,Roets E,Hoogmartens J

    更新日期:1996-06-01 00:00:00

  • Development of a capillary electrophoresis assay for the determination of carvedilol enantiomers in serum using cyclodextrins.

    abstract::A capillary electrophoresis method using cyclodextrins as the chiral selectors was developed for the determination of carvedilol enantiomers in serum. Several types of cyclodextrins were evaluated. The effect of cyclodextrin concentration on enantiomer resolution was investigated. Best results were obtained using 10 m...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章


    authors: Clohs L,McErlane KM

    更新日期:2001-02-01 00:00:00

  • Microspectroscopic investigation of the membrane clogging during the sterile filtration of the growth media for mammalian cell culture.

    abstract::Growth media for mammalian cell culture are very complex mixtures of several dozens of ingredients, and thus the preparation of qualified media is critical to viable cell density and final product titers. For liquid media prepared from powdered ingredients, sterile filtration is required prior to use to safeguard the ...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章


    authors: Cao X,Loussaert JA,Wen ZQ

    更新日期:2016-02-05 00:00:00

  • Instrumentation of a handy microscopic probe for concurrent observation and measurement of active sweat secretion, and its applications.

    abstract::Instrumentation for the concurrent, dynamic monitoring of active sweat glands and perspiration volume is described. A device for the measurement of the rate of sweat secretion was installed on the head part of a microscope. The combined apparatus (microscopic probe) is handy for use and its weight is very light (ca. 3...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 临床试验,杂志文章


    authors: Kamei T,Naitoh K,Nakashima K,Ohhashi T,Kitagawa S,Tsuda T

    更新日期:1997-06-01 00:00:00

  • Determination of nucleoside analog mono-, di-, and tri-phosphates in cellular matrix by solid phase extraction and ultra-sensitive LC-MS/MS detection.

    abstract::An ultra-sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) assay was developed and validated to facilitate the assessment of clinical pharmacokinetics of nucleotide analogs from lysed intracellular matrix. The method utilized a strong anion exchange isolation of mono-(MP), di-(DP), and tri-phosphates...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章


    authors: Bushman LR,Kiser JJ,Rower JE,Klein B,Zheng JH,Ray ML,Anderson PL

    更新日期:2011-09-10 00:00:00

  • Chemically modified carbon paste electrode for the potentiometric flow injection analysis of piribedil in pharmaceutical preparation and urine.

    abstract::A new carbon paste electrode selective for piribedil (PD) was prepared and fully characterized in terms of composition, usable pH range, response time and thermal stability. The electrode active recognition is by liquid ion-exchange mechanism via the use of piribedil phosphomolybdate as ion-exchanger dissolved in tric...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章


    authors: Ibrahim H

    更新日期:2005-07-15 00:00:00

  • Isotope-coded derivatization based LC/ESI-MS/MS methods using a pair of novel reagents for quantification of hydroxycinnamic acids and hydroxybenzoic acids in fermented brown rice product.

    abstract::Hydroxycinnamic acids (HCAs) and hydroxybenzoic acids (HBAs) are antioxidant phytochemicals found in rice and effective for the prevention of human diseases including cancer. FBRA, which is a functional food manufactured by fermenting brown rice and rice bran with Aspergillus oryzae, has been demonstrated to have chem...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章


    authors: Ogawa S,Takafuji K,Tsubuku S,Horie Y,Ikegawa S,Higashi T

    更新日期:2017-08-05 00:00:00

  • Stereochemistry of pipecolic acid found in the urine and plasma of subjects with peroxisomal deficiencies.

    abstract::Recently it was found that normal adults excrete pipecolic acid primarily as the D-enantiomer even though it is present in the blood stream mainly as the L-enantiomer (i.e. > 98% L). This study of pipecolic acid stereochemistry was extended to subjects with peroxisomal deficiencies since they are known to have high le...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章


    authors: Armstrong DW,Zukowski J,Ercal N,Gasper M

    更新日期:1993-10-01 00:00:00

  • Flow-through cross-polarized imaging as a new tool to overcome the analytical sensitivity challenges of a low-dose crystalline compound in a lipid matrix.

    abstract::Assessing the physical state of a low-dose active compound in a solid lipid or polymer matrix is analytically challenging, especially if the matrix exhibits some crystallinity. The aim of this study was first to compare the ability of current methods to detect the presence of a crystalline model compound in lipid matr...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章


    authors: Adler C,Schönenberger M,Teleki A,Leuenberger B,Kuentz M

    更新日期:2015-11-10 00:00:00

  • Development of a solid-phase binding assay and identification of nonpeptide ligands for the FynB Src homology 2 domain.

    abstract::The nonreceptor tyrosine kinase FynB is known to be required in the induction of long-term potentiation (LTP), a cellular mechanism for learning and memory. Ligands of the FynB SH2 domain as a possible FynB activator are, thus, of great interest. In this study, a solid-phase ligand binding assay was established to mee...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章


    authors: Kim HJ,Lee HH,Yoo HD,Hwa Lee J,Hong ST

    更新日期:2002-01-01 00:00:00

  • Development of a chiral assay for a novel, nonfluorinated quinolone, PGE-9509924, in dog plasma using high performance liquid chromatography with electrospray tandem mass spectrometry or fluorescence detection.

    abstract::PGE-9509924 is a nonfluorinated quinolone and is active against a variety of susceptible and drug resistant bacteria in vitro and in animal infection models. A method for determining both enantiomers of PGE-9509924 in dog plasma has been developed. The enantiomers are derivatized with a chiral derivatizing agent, (-)-...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章


    authors: Zoutendam PH,Canty JF,Martin MJ,Dirr MK

    更新日期:2002-08-22 00:00:00

  • Distribution and metabolism of gastrodin in rat brain.

    abstract::Gastrodin is the major and bioactive component in Tianma (Gastrodia elata Bl.) and has sedative, anticonvulsive and neuroprotective effects. Since little is known about its neuropharmacokinetics and brain metabolism, this study was undertaken to investigate the kinetic inter-relationship of gastrodin in rat plasma, ce...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章


    authors: Wang Q,Chen G,Zeng S

    更新日期:2008-01-22 00:00:00

  • Liquid chromatographic-tandem mass spectrometric method for the simultaneous quantitation of telmisartan and hydrochlorothiazide in human plasma.

    abstract::A rapid and sensitive method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed for the simultaneous determination of telmisartan and hydrochlorothiazide in human plasma. Sample preparation involved liquid-liquid extraction with diethyl ether-dichloromethane (60:40, v/v). The analytes a...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章


    authors: Yan T,Li H,Deng L,Guo Y,Yu W,Fawcett JP,Zhang D,Cui Y,Gu J

    更新日期:2008-12-01 00:00:00

  • Statistical approaches to determine analytical variability and specifications: application of experimental design and variance component analysis.

    abstract::Assessment of analytical variability is recognized as an important factor for the establishment of specifications. Estimation of the variance for an analytical procedure can be accomplished using a variety of approaches. The approach of variance component analysis was applied retrospectively, as well as prospectively,...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章


    authors: Tsang PK,Larew JS,Larew LA,Miyakawa TW,Hofer JD

    更新日期:1998-03-01 00:00:00

  • Potentiometric sensor for methylene blue based on methylene blue-silicotungstate ion association and its pharmaceutical applications.

    abstract::A methylene blue (MB) poly(vinyl chloride) membrane sensor based on MB-silicotungstate (SLT) ion association as electroactive material was described. The linear response covered the range 1 x 10(-3)-1 x 10(-6) mol x dm(-3) MB solution, with a slope 52.0+/-0.8 mV decade(-1) (pH range 3.0-10.0). The detection limit was ...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章


    authors: Wen ML,Zhao YB,Chen X,Wang CY

    更新日期:1999-01-01 00:00:00

  • Authentication of Eleutherococcus and Rhodiola herbal supplement products in the United Kingdom.

    abstract::Siberian ginseng (Eleutherococcus senticosus, Araliaceae) and roseroot (Rhodiola rosea, Rosaceae) are popular herbal supplements which have been shown to improve resilience to conditions such as stress and exhaustion. Using DNA barcoding methods we tested 25 Siberian ginseng and 14 roseroot products which are widely a...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章


    authors: Ruhsam M,Hollingsworth PM

    更新日期:2018-02-05 00:00:00

  • Chiral analysis of drug substance in clinical plasma extracts using achiral HPLC with circular dichroism detection.

    abstract::A circular dichroism (CD) detector in series with a UV detector was used with reversed-phase gradient HPLC to do the chiral analysis of unresolved enantiomers of a single stereoisomer drug substance in extracts of clinical plasma samples. The CD/UV peak area ratio of the unresolved enantiomers in the plasma extracts w...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章


    authors: Williams RC,Edwards JF,Joshi AS,Aubry AF

    更新日期:2001-06-01 00:00:00

  • Application of stripping voltammetry to trace lead analysis in intermediates and final products of syntheses of pharmaceuticals.

    abstract::Applications of differential pulse anodic stripping voltammetry using a new pen-type renewed hanging mercury electrode have been investigated for trace analysis of lead in pharmaceutical substances and intermediates of their syntheses, such as procaine hydrochloride, 4-aminobenzoic acid, methyl 4-aminobenzoate, 2-(4-c...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章


    authors: Pravda M,Vytras K

    更新日期:1996-05-01 00:00:00

  • An improved LC-S/MS method for the quantitation of adenosine concentration in mice brain microdialysates.

    abstract::A sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the determination of adenosine concentrations in mouse brain microdialysis samples was developed. High method sensitivity (LLOQ of 1.25 fmol) was achieved by on-line switching column. A C18 was employed as enrichment column and a cyano ba...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章


    authors: Carrozzo MM,Troisi L,Cannazza G,Cazzato AS,Braghiroli D,Parenti C,Guiducci S,Zoli M

    更新日期:2012-11-01 00:00:00

  • Metabolic profiles of neotuberostemonine and tuberostemonine in rats by high performance liquid chromatography/quadrupole time-of-flight mass spectrometry.

    abstract::Neotuberostemonine (NS) and tuberostemonine (TS), a pair of stereoisomers, are the active components contained in Stemona tuberosa, an antitussive herbal medicine in China. Two isomers have different pharmacological efficacies, which will be related with their in vivo disposition. However, the metabolic fates of NS an...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章


    authors: Tong Y,Xu W,Wu Y,Ou L,Zhang M,Xu X,Zhang C

    更新日期:2017-07-15 00:00:00

  • Inflammatory markers in dependence on the plasma concentration of 37 fatty acids after the coronary stent implantation.

    abstract::Using the regression model building the relationships between the concentration of 37 fatty acids of blood plasma phospholipids of 41 patients with coronary artery disease after coronary stent implantation, the inflammatory response and oxidative stress markers were estimated. The dynamics of the inflammatory response...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章


    authors: Handl J,Meloun M,Mužáková V

    更新日期:2018-02-05 00:00:00

  • Strategy for design NIR calibration sets based on process spectrum and model space: An innovative approach for process analytical technology.

    abstract::The pharmaceutical industry is under stringent regulations on quality control of their products because is critical for both, productive process and consumer safety. According to the framework of "process analytical technology" (PAT), a complete understanding of the process and a stepwise monitoring of manufacturing a...

    journal_title:Journal of pharmaceutical and biomedical analysis

    pub_type: 杂志文章


    authors: Cárdenas V,Cordobés M,Blanco M,Alcalà M

    更新日期:2015-10-10 00:00:00