Abstract：
:A rapid, sensitive and high throughput UHPLC-MS/MS method was established and validated to assay the concentration of 2-(2-hydroxypropanamido) benzoic acid (HPABA), a promising anti-inflammatory drug, in rat plasma. Plasma samples were processed by liquid-liquid extraction with ethyl acetate and separated on a Shim-pack XR-ODS C18 column (75 mm×3.0 mm, 2.2 μm) at an isocratic flow rate of 0.4 mL/min using acetonitrile-0.1% formic acid in water (50:50, v/v) as mobile phase, and total run time was 2 min. MS/MS detection was accomplished in multiple reaction monitoring (MRM) mode with positive electrospray ionization. The calibration curve was linear over the concentration range of 0.01-50 μg/mL with lower limit of quantification of 0.01 μg/mL. The intra- and inter-day precisions were below 8.5% in terms of relative standard deviation (RSD), and the accuracy was within ±4.0% in terms of relative error (RE). Extraction recovery, matrix effect and stability were satisfactory in rat plasma. The developed method was successfully applied to a pharmacokinetic study of HPABA following intragastric administration of 25, 50, 100mg/kg and an intravenous injection at a dose of 12.5 mg/kg to Sprague-Dawley rats. Results indicated that HPABA had linear pharmacokinetic properties within the tested intragastric dosage range and the absolute bioavailability was above 59.1%.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
Guan J,Zhao Y,Zhu H,An Z,Yu Y,Li R,Yu Zdoi
10.1016/j.jpba.2014.02.008subject
Has Abstractpub_date
2014-07-01 00:00:00pages
20-5eissn
0731-7085issn
1873-264Xpii
S0731-7085(14)00099-5journal_volume
95pub_type
杂志文章abstract：:A simple, sensitive and robust method using HILIC-ESI-MS has been developed for the determination of methane sulfonic acid (MSA) at low ppm level in order to verify the effectiveness of controlling the formation of genotoxic sulfonate esters in the downstream synthetic step, by which produces active pharmaceutical ing...
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journal_title：Journal of pharmaceutical and biomedical analysis
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journal_title：Journal of pharmaceutical and biomedical analysis
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journal_title：Journal of pharmaceutical and biomedical analysis
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journal_title：Journal of pharmaceutical and biomedical analysis
pub_type： 杂志文章,评审
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journal_title：Journal of pharmaceutical and biomedical analysis
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