Abstract:
:A direct and sensitive liquid chromatographic method for the determination of propofol in 50 microliters of plasma is described. The separation of the drug and internal standard (methyldopa) was achieved using a 4 microns particle size C1R cartridge (10 cm x 8 mm i.d.) in conjunction with a radial compression system and a C18 precolumn module. The mobile phase consisted of 0.01 M sodium acetate solution (adjusted to pH 3 with acetic acid)-acetonitrile-methanol (37:47.25:15.75, v/v/v) at a flow rate of 2 ml min-1. The compounds were detected in the effluent spectrofluorimetrically with excitation and emission wavelengths of 276 and 310 nm, respectively. After the internal standard had been added, the sample was diluted with 50 microliters of hydrochloric acid and centrifuged prior to injection into the chromatograph. The peaks of both propofol and internal standard under these conditions were sharp and symmetrical, and the retention times were 8.2 and 5.15 min, respectively. The peak-height ratio (drug/internal standard) varied linearly (r > 0.9959) with concentration in the ranges 0.002-0.1 and 0.1-10 micrograms ml-1 and the relative standard deviation was consistently < 5.6%. There was no interference in the assay from the endogenous substance or other concomitantly used drug. This method is currently being used for monitoring propofol in intensive care patients and investigating its pharmacokinetics.
journal_name
J Pharm Biomed Analjournal_title
Journal of pharmaceutical and biomedical analysisauthors
el-Yazigi A,Hussein RFdoi
10.1016/0731-7085(96)01818-3subject
Has Abstractpub_date
1996-10-01 00:00:00pages
99-104issue
1eissn
0731-7085issn
1873-264Xpii
0731-7085(96)01818-3journal_volume
15pub_type
杂志文章abstract::Adulterations with synthetic drugs are common problems with herbal medicine and this can potentially cause serious adverse effects. It is therefore important to determine the presence of synthetic drugs in herbal medicine to ensure patients' safety. The objective of this study was to develop sensitive and specific met...
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