Abstract:
:Listeria monocytogenes is able to survive and proliferate within macrophages. In the current study, the ability of three L. monocytogenes strains (serovar 1/2a strain EGDe, serovar 4b strain F2365, and serovar 4a strain HCC23) to proliferate in the murine macrophage cell line J774.1 was analyzed. We found that the avirulent strain HCC23 was able to initiate an infection but could not establish prolonged infection within the macrophages. By contrast, strains EGDe and F2365 proliferated within macrophages for at least 7 h. We further analyzed these strains by comparing their protein expression profiles at 0 h, 3 h, and 5 h post-infection using multidimensional protein identification technology coupled with electrospray ionization tandem mass spectrometry. Our results indicated that similar metabolic and cell wall associated proteins were expressed by all three strains at 3 h post-infection. However, increased expression of stress response and DNA repair proteins was associated with the ability to proliferate in macrophages at 5 h post-infection. By comparing the protein expression patterns of these three L. monocytogenes strains during intracellular growth in macrophages, we were able to detect biological differences that may determine the ability of L. monocytogenes to survive in macrophages.
journal_name
J Proteomicsjournal_title
Journal of proteomicsauthors
Donaldson JR,Nanduri B,Pittman JR,Givaruangsawat S,Burgess SC,Lawrence MLdoi
10.1016/j.jprot.2011.05.008subject
Has Abstractpub_date
2011-09-06 00:00:00pages
1906-17issue
10eissn
1874-3919issn
1876-7737pii
S1874-3919(11)00199-0journal_volume
74pub_type
杂志文章abstract::Sex-biased protein is thought to be able to drive the phenotypic differences in males and females in insects. In this study, 1385 and 1727 proteins were identified as differentially accumulated proteins (DAPs) by comparing the protein abundances at pupae stage with those at adult stage in ovary and testis of S.litura,...
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abstract::Organic farming aims to be environmentally sound, but the question as to whether organic cropping brings more nutritional benefits to farmers and consumers than the conventional cropping needs still to be answered. To gain insights into the molecular effects of organic farming we used proteome analysis to analyze cabb...
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journal_title:Journal of proteomics
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doi:10.1016/j.jprot.2016.02.026
更新日期:2016-04-14 00:00:00
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journal_title:Journal of proteomics
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doi:10.1016/j.jprot.2011.06.013
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journal_title:Journal of proteomics
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journal_title:Journal of proteomics
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journal_title:Journal of proteomics
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doi:10.1016/j.jprot.2011.05.032
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abstract::Several proteomics studies have been conducted to identify new cerebrospinal fluid (CSF) biomarkers in Multiple Sclerosis (MuS). However, the complexity of CSF and its invasive collection, limits its use. Therefore, the goal of biomarker research in MuS is to identify novel distinctive targets in CSF or in easily acce...
journal_title:Journal of proteomics
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journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2014.05.023
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pub_type: 杂志文章
doi:10.1016/j.jprot.2017.09.004
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journal_title:Journal of proteomics
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journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2013.10.017
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journal_title:Journal of proteomics
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doi:10.1016/j.jprot.2008.11.012
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journal_title:Journal of proteomics
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journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2013.05.037
更新日期:2013-08-26 00:00:00
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journal_title:Journal of proteomics
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journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2011.05.036
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journal_title:Journal of proteomics
pub_type: 历史文章,杂志文章,评审
doi:10.1016/j.jprot.2014.03.038
更新日期:2014-07-31 00:00:00
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journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2019.103600
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journal_title:Journal of proteomics
pub_type: 杂志文章
doi:10.1016/j.jprot.2010.09.002
更新日期:2010-10-10 00:00:00
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journal_title:Journal of proteomics
pub_type: 杂志文章
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